质粒名称: | pLenti-sgRNA |
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出品公司: | 爱迪基因 |
目录编号: | 71409 |
存储实验室: | Eric Lander, David Sabatini |
载体骨架基本信息 |
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载体名称: | pLenti-CRISPR |
载体出品公司: | Feng Zhang |
载体抗性: | 氨苄青霉素 |
空载体大小: | 9100 bp |
完整质粒大小: | -- |
载体修饰: | removed Cas9 from Feng Zhang's pLentiCRISPR plasmid |
载体类型: | 慢病毒载体 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | -- |
生长条件: | 30 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pLenti-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 71409 ; http://n2t.net/addgene:71409 ; RRID:Addgene_71409) |
买家导航
pLentisgRNA载体基本信息
pLentisgRNA载体图谱质粒图谱和多克隆位点信息
pLentisgRNA载体简介
pLentisgRNA载体序列
LOCUS pLenti-sgRNA 9151 bp ds-DNA circular SYN 21-JAN-2021 DEFINITION There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI. ACCESSION . VERSION . KEYWORDS pLenti-sgRNA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9151) TITLE pLenti-sgRNA JOURNAL Unpublished REFERENCE 2 (bases 1 to 9151) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9151 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 138..157 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 391..408 /label=L4440 /note="L4440 vector, forward primer" protein_bind 525..546 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 561..591 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 599..615 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 604..626 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 623..639 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 623..639 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 658..678 /label=T3 /note="T3 promoter, forward primer" promoter 660..678 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 706..932 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 933..1113 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1160..1285 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1778..2011 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." promoter 2418..2658 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 2418..2438 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 2589..2608 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 4548..4623 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 4671..4788 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 4857..5068 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" CDS 5094..5693 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5094..5113) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 5590..5610 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5709..6297 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5762..5782) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(6180..6191) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 6369..6602 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6680..6801 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6717..6736) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6771..6790 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6841..6976 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6903..6922 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6996..7015) /label=T7 /note="T7 promoter, forward primer" promoter complement(6997..7015) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(7025..7042) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(7025..7041) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7034..7056) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 7183..7638 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7270..7289) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7480..7501 /label=F1ori-F /note="F1 origin, forward primer" promoter 7664..7768 /gene="bla" /label=AmpR promoter CDS 7769..8629 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7987..8006) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8800..237 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 61 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac 481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc 541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 601 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca 661 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa 721 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga 781 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa 841 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata 901 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag 961 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 1021 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 1081 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc 1141 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc 1201 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa 1261 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga 1321 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc 1381 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg 1441 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc 1501 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac 1561 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca 1621 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat 1681 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga 1741 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct 1801 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac 1861 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac 1921 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg 1981 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca 2041 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt 2101 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac 2161 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat 2221 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa 2281 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt 2341 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa 2401 ccccgagggg acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat 2461 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac 2521 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt 2581 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat 2641 atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac aaaatacaca 2701 tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct ttttaataac 2761 tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat aaaaatgagc 2821 aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt aataatagct 2881 actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg agcatcttca 2941 aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac tgacttcatt 3001 agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt tttaacaaat 3061 tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt tgttaataaa 3121 caattttcca taatacattt aacaacatgt gatccagctg ctttttttac agctttcatg 3181 tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa taccatatca 3241 atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt agctgttgtg 3301 catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc ttttaataat 3361 tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc aaattcaatt 3421 gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa taatgtgtga 3481 tctatatatt tgtttagttt cattttttct cctatatatt catttttaat tttaattctt 3541 taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca cctataaaat 3601 aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac ttatcttcta 3661 aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg tagtcttcaa 3721 cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat gatttcatta 3781 atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa gtgaaagtgt 3841 aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt ttattttgtt 3901 ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca tctttatctc 3961 tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca aaatttaatc 4021 cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt aaagttttta 4081 caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt gttacaaaac 4141 ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt gataattttc 4201 aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga ccatcatgtt 4261 ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata tctgcatcat 4321 gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga tttggattta 4381 caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata acgttatatc 4441 ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact gcgctaaaaa 4501 taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt ttagagctag 4561 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg 4621 tgcttttttg aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga 4681 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac 4741 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac 4801 agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc gtcagtgggc 4861 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg 4921 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 4981 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 5041 tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gggatccgcc accatgaccg 5101 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc 5161 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca 5221 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca 5281 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg 5341 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc 5401 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt 5461 ggttcctggc caccgtcgga gtctcgcccg accaccaggg caagggtctg ggcagcgccg 5521 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct 5581 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg 5641 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgaacgcgtt 5701 aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 5761 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 5821 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 5881 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 5941 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 6001 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 6061 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt 6121 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 6181 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 6241 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc 6301 gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa 6361 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac 6421 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6481 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6541 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6601 cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 6661 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 6721 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 6781 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 6841 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 6901 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6961 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc 7021 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 7081 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg 7141 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg 7201 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc 7261 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc 7321 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg 7381 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg 7441 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg 7501 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt 7561 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa 7621 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 7681 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 7741 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 7801 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 7861 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 7921 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 7981 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 8041 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 8101 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 8161 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 8221 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 8281 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 8341 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 8401 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 8461 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 8521 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 8581 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 8641 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 8701 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 8761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 8821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 8881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 8941 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 9001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 9061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 9121 ggggggttcg tgcacacagc ccagcttgga g //
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pLentisgRNA载体质粒应用举例
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