LOCUS pYS1 11799 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Generation of unmarked gene deletions in Mycobacterium smegmatis.
ACCESSION .
VERSION .
KEYWORDS pYS1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11799)
AUTHORS Shenkerman Y, Elharar Y, Vishkautzan M, Gur E
TITLE Efficient and simple generation of unmarked gene deletions in
Mycobacterium smegmatis.
JOURNAL Gene. 2014 Jan 1;533(1):374-8. doi: 10.1016/j.gene.2013.09.082. Epub
2013 Oct 4.
PUBMED 24100088
REFERENCE 2 (bases 1 to 11799)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11799
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 214..646
/gene="sacR"
/label=sacB promoter
/note="sacB promoter and control region"
CDS 647..2068
/codon_start=1
/gene="Bacillus subtilis sacB"
/product="secreted levansucrase that renders bacterial
growth sensitive to sucrose"
/label=SacB
/note="negative selection marker"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
rep_origin complement(2235..2823)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2315..2334)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5967..6782)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 6690..6709
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
terminator 6943..6989
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ggtaccgata tctgatttag gatacatttc tagccacctg acgtcggggg gggggggaaa
61 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
121 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
181 aacgggaaac gtcttgctcg accatatacc tgccgttcac tattatttag tgaaatgaga
241 tattatgata ttttctgaat tgtgattaaa aaggcaactt tatgcccatg caacagaaac
301 tataaaaaat acagagaatg aaaagaaaca gatagatttt ttagttcttt aggcccgtag
361 tctgcaaatc cttttatgat tttctatcaa acaaaagagg aaaatagacc agttgcaatc
421 caaacgagag tctaatagaa tgaggtcgaa aagtaaatcg cgcgggtttg ttactgataa
481 agcaggcaag acctaaaatg tgtaaagggc aaagtgtata ctttggcgtc accccttaca
541 tattttaggt ctttttttat tgtgcgtaac taacttgcca tcttcaaaca ggagggctgg
601 aagaagcaga ccgctaacac agtacataaa aaaggagaca tgaacgatga acatcaaaaa
661 gtttgcaaaa caagcaacag tattaacctt tactaccgca ctgctggcag gaggcgcaac
721 tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag gaaacatacg gcatttccca
781 tattacacgc catgatatgc tgcaaatccc tgaacagcaa aaaaatgaaa aatatcaagt
841 tcctgaattc gattcgtcca caattaaaaa tatctcttct gcaaaaggcc tggacgtttg
901 ggacagctgg ccattacaaa acgctgacgg cactgtcgca aactatcacg gctaccacat
961 cgtctttgca ttagccggag atcctaaaaa tgcggatgac acatcgattt acatgttcta
1021 tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac gctggccgcg tctttaaaga
1081 cagcgacaaa ttcgatgcaa atgattctat cctaaaagac caaacacaag aatggtcagg
1141 ttcagccaca tttacatctg acggaaaaat ccgtttattc tacactgatt tctccggtaa
1201 acattacggc aaacaaacac tgacaactgc acaagttaac gtatcagcat cagacagctc
1261 tttgaacatc aacggtgtag aggattataa atcaatcttt gacggtgacg gaaaaacgta
1321 tcaaaatgta cagcagttca tcgatgaagg caactacagc tcaggcgaca accatacgct
1381 gagagatcct cactacgtag aagataaagg ccacaaatac ttagtatttg aagcaaacac
1441 tggaactgaa gatggctacc aaggcgaaga atctttattt aacaaagcat actatggcaa
1501 aagcacatca ttcttccgtc aagaaagtca aaaacttctg caaagcgata aaaaacgcac
1561 ggctgagtta gcaaacggcg ctctcggtat gattgagcta aacgatgatt acacactgaa
1621 aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca gatgaaattg aacgcgcgaa
1681 cgtctttaaa atgaacggca aatggtacct gttcactgac tcccgcggat caaaaatgac
1741 gattgacggc attacgtcta acgatattta catgcttggt tatgtttcta attctttaac
1801 tggcccatac aagccgctga acaaaactgg ccttgtgtta aaaatggatc ttgatcctaa
1861 cgatgtaacc tttacttact cacacttcgc tgtacctcaa gcgaaaggaa acaatgtcgt
1921 gattacaagc tatatgacaa acagaggatt ctacgcagac aaacaatcaa cgtttgcgcc
1981 aagcttcctg ctgaacatca aaggcaagaa aacatctgtt gtcaaagaca gcatccttga
2041 acaaggacaa ttaacagtta acaaataagc ggccgctcgc tgcgctcggt cgttcggctg
2101 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
2161 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
2221 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
2281 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
2341 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
2401 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
2461 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
2521 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
2581 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
2641 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
2701 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
2761 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
2821 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga gtatcgagcc
2881 gagaacgtta tcgaagttag tcatgtgtaa tcccctcgtt tgaactttgg attaagcgta
2941 gatacaccct tggacaagcc agttggattc ggagacaagc aaattcagcc ttaaaaaggg
3001 cgaggccctg cggtggtgga acaccgcagg gcctctaacc gctcgacgcg ctgcaccaac
3061 cagcccgcga acggctggca gccagcgtaa ggcgcggctc atcgggcggc gttcgccacg
3121 atgtcctgca cttcgagcca agcctcgaac acctgctggt gtgcacgact cacccggttg
3181 ttgacaccgc gcgcggccgt gcgggctcgg tggggcggct ctgtcgccct tgccagcgtg
3241 agtagcgcgt acctcacctc gcccaacagg tcgcacacag ccgattcgta cgccataaag
3301 ccaggtgagc ccaccagctc cgtaagttcg ggcgctgtgt ggctcgtacc cgcgcattca
3361 ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg ccgccacagc ggctctcagc
3421 ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct ggttggtaca ggtggttggg
3481 ggtgctcggc tgtcgctggt gttccaccac cagggctcga cgggagagcg ggggagtgtg
3541 cagttgtggg gtggcccctc agcgaaatat ctgacttgga gctcgtgtcg gaccatacac
3601 cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt cgccgacgaa tttgagcagc
3661 tctggctgcc gtactggccg ctggcaagcg acgatctgct cgaggggatc taccgccaaa
3721 gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa cccaacagcg ctggcaaacc
3781 tgctggtcgt ggacgtagac catccagacg cagcgctccg agcgctcagc gcccgggggt
3841 cccatccgct gcccaacgcg atcgtgggca atcgcgccaa cggccacgca cacgcagtgt
3901 gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg gcgtaagccg ctcgcataca
3961 tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgacgg cgaccgcagt tactcaggcc
4021 tcatgaccaa aaaccccggc cacatcgcct gggaaacgga atggctccac tcagatctct
4081 acacactcag ccacatcgag gccgagctcg gcgcgaacat gccaccgccg cgctggcgtc
4141 agcagaccac gtacaaagcg gctccgacgc cgctagggcg gaattgcgca ctgttcgatt
4201 ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat ctacctaccg acccggaacg
4261 tggacggact cggccgcgcg atctatgccg agtgccacac gcgaaacgcc gaattctcgt
4321 gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt ccgcgccatc gccaacagca
4381 tttggcgttg gatcacaacc aagtcgcgca tttgggcgga cgggatcgtg gtctacgagg
4441 ccacactcag tgcgcgccag tcggccatct cgcggaaggg cgcagcagcg cgcacggcgg
4501 cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat ggaggcattg ctatgagcga
4561 cggctacagc gacggctaca accggcagcc gactgtccgc aaaaagcggc gcgtgaccgc
4621 caccgaaggc gctcgaatca ccggactatc cgaacgccac gtcgtccggc tcgtggcgca
4681 ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc gaacgcatcc gcgcctatca
4741 cgacgacgag ggccactctt ggctgcaaac ggccaaacat ttcgggctgc atctggacac
4801 cgttaagcga ctcggctatc gggcaaggaa agagcgtgcg gcagaacagg aagcggctca
4861 aaaggcccac aacgaagccg acaatccacc gctgttctaa cgcaattggg gagcgggtgt
4921 cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga caggtaaaag tcctggtaga
4981 cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt gtttcgttgc gcccgttttg
5041 aataccagcc agacgagacg gggttctacg aatcttggtc gataccaagc catttccgct
5101 gaatatcggg gagctcaccg ccagaatcgg tggttgtggt gatgtacgtg gcgaactccg
5161 ttgtagtgcc tgtggtggca tccgtggcca ctctcgttgc acggttcgtt gtgccgttac
5221 aggccccgtt gacagctcac cgaacgtagt taaaacatgc tggtcaaact aggtttacca
5281 acgatacgag tcagctcatc tagggccagt tctaggcgtt gttcgttgcg cggttcgttg
5341 cgcatgtttc gtgtggttgc tagatggctc cgcaaccaca cgcttcgagg ttgagtgctt
5401 ccagcacggg cgcgatccag aagaacttcg tcgtgcgact gtcctcgtta acgctcgaag
5461 cgatgccgat caaatcggct gtgccgtcgc ggttcttgcg gtacagcggc gcgcctgagt
5521 cgccatgtac ggtgcgcagc tgagcggtga tcgtgtactt ggcagccaca acgaccggcc
5581 cacaggtctc gccggatttc atcccgtact tgcagacctc ggtgccgtac ggaacatctt
5641 tgcggtcctt cagcaccgca cgcacggggt atcttccgcc gattttgaac gaagcgatcg
5701 gtgttcccgg agcaaacgca atcaccccag cgtcggtgta gaccggcaat agatcatcgt
5761 cgccggtaat cgtggtgcct tcgtctctcg acgcaacgta ggtacccaac gtccgctgtg
5821 tatccggttt ctctgcaacc gaatacagca ccgggccccc cctcgagttt aaacattttt
5881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccatgcatgt gctagcccag
5941 tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaattgc
6001 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
6061 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
6121 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
6181 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag attatgcatt
6241 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
6301 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta
6361 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
6421 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
6481 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
6541 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
6601 acgctacctt tgccatgttt cagaaacaac tctggcgcat ggggcttccc atacaagcga
6661 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
6721 gcatccatgt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg aatatggctc
6781 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
6841 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttcccccccc
6901 cccgacgtca ggtggctagc tgatcaccgc ggccatgatg gcataaaacg aaaggcccag
6961 tctttcgact gagcctttcg ttttatttga tgcctggcag tcgatcgtac gctagttgca
7021 gatcaaccac cgcgtcggaa ttcgccgggg cgctcatgcg ttgggcccgt cgaacatgtc
7081 gatgacagcc ttcgcctcgt cgaacgtgag gtcggcggcg cgcgtggctt tgacgccggc
7141 cgaatcggcg aggaacttga accaatcggc gtcgttgtac ttttcggcct tctggatctc
7201 gccgaggcgc ttcagctgat cgggattggc catctgcacg tcgggcgccg gcgcggtggc
7261 gcgctgcgcg cgttgtgcgc gcgacgactt cggtggcgta tcgacctcgt gcggctcggc
7321 gatctcgccg tcgatgtagt cggccgtcgg cttgacggtc gacggcagcg cgagcgcctg
7381 cgactgtgac aggtcggtgc ccgggcgatc gtcggcgcgg atcgccgcgt cgagccgcgt
7441 cgtcttcggt gccagcttca gcacctgttt gagcgcggtc ttccgctcca tccagcgctg
7501 cgggtccttg atgtcgcccg aggatccgac cttgccgcga cgcaattccc tgatctcgtc
7561 ggcggtgaac acgtcccaca gcggctcagc gccggtcacc tcgacgatcg cgtagaagta
7621 gaccggatta ccccgatcac ccttggcctt cacgtgtttc agcgtcggat tgaggcccat
7681 ggtgtaatgg aattcgtcgt tcgcgccgac ccactgcgcg tcgatccggg aggcgcgcgg
7741 gtgctgccag aacagtttga cgatgccctg gtagccgatg atcagctggc actcgaccac
7801 gccgcgccgg gtgtcgcggt agggcacgag gtagcactcg ccgttgacac cgggctcgag
7861 accgagcgcg gccgaggtca gcagcgtacc ggcaaatgac tcttgcgtgc agtcgtcgag
7921 ggactgctta gcgatgccag cagctttcgc gttgcgcgac ttgcggatct cggtttgcac
7981 gatccgcgcg atgcggtcgg ggtccatacc cttgggcact gcacgcgcca gctgcggcac
8041 gagcacctgc agcacttgcg agatcgtctc gggtgccttg ggcgaatcct gcttggtgac
8101 agcattttca gccatagatg taatcccctt caatcgggtc ggcctcttcg agcgggtcgg
8161 tgtcgtagat atagctgtcg gtgacgacgt cgccgacggt ctgcggtcgc ggcagccccc
8221 acgagggcag gctgatcgtg accacgtcgt cgctgtagtc aggccacttg cccgattcca
8281 tgcagtcggc gtagagccgg atcgcctggc ggttgcggcg tcgcccctct tcgatcgcct
8341 cgtcgtcgta ctggatcgga gtcacgacgt agggcggttc tttctcctgc gtgatgaacc
8401 ggaatcgcgg gttctcggcg agcccgaggg cgaccagtag atcgatgtac caggccgcct
8461 gcatgaaata gccgagcttg tagaacttgg ttttcagctc ggccgggttc gcggtcatcg
8521 acgtcttgta atcatcgata tcgtcagtga gccagtcgat ccggccacgc agccgcacgc
8581 cggtctcggg gtcggtgtag tacagcgcga cctcggcctc gccgtcagga ttggcgaaga
8641 tcgggccggc tgtcgggtgc tgacgcacct tttcggccat gtcgtacgcc ttcgtgaaca
8701 ggtcgacgtg aatcggcacc ttgccctgtt tgcgagcctc agcctcggcc ttgcgccaca
8761 tgcccgtcgc ggtcggcttc tccgacggcg taccgtccgc cttcagcccg tgcacctcgg
8821 ggtcgaggat ctcgaactcg gcacccttgc cgagcaccag tttgtgcgcg acatgtccga
8881 agtcccagac ctttttcggc ttccgggtgt tgtccatctc ccagcggaat ttcgcgggac
8941 acgacggcgg caacagcagc ttcgcgccgg ataccgacag cgagccccgg tcagcgtggt
9001 agacgtcctc gtcgacgaac cggtgcattc cgtcctgtgt gggcacactc atatgcggcc
9061 gccgatctcg taactacgtc gacatcgata agcttcgaat tctgcagctg gatccgttac
9121 atatgtggac tccctttctc ttatcgggtg gtggccgcga gcgcggccgg tggagggttg
9181 gtgactgacg gcgctgcgag acggcgcccg atggggtatg cgagaacaac tgcggcaaaa
9241 ccgatcacgg cgatgaccgc ggcggggagc gggccggttt cccagttgcc cgacacgatc
9301 aggaaggccg gcacggcggc ggtgatcacg ccttcggcca ccgcgacgaa cccgacggcg
9361 ggccccagtg cgtcgtgccc caggccgagc agcaggaaca gcatgaacca cagcactgcc
9421 cacagcagcc agatcacccc gaacgccggg tcggcctcgg cggtgaacgc gtgccacgag
9481 tagccgagtg cggcgatcgc gacgaacagc gagaaccatc cgagaccttc tccgtcccag
9541 tcggtcacgt tgttgatggc cacccacagg taggtgaagc cgaacaggta gagcccggag
9601 gccgcgaaga tcacggccgc gtctccgccg gactgcagga tcagcaccgt aggcgtcacc
9661 acctgcagtc cgccgacgaa gaagttcagc ggtgttgcgc ctcgtgggct gatgcggccc
9721 agcagcatca gcccgtcgat gatgagcacc gcacccacgt agaagagccc gacgccaccc
9781 atgtcgctag tacctcggca gactcggtag accgggattg cggcgcgggg cccgcgggcg
9841 gtccgcgcct gcgggaatcg acttcaccac cgtgggactt tcggcgctcg cggacgccgc
9901 gtcgaatgcg cggtgatgtc ccgcggtgaa tgtcgtcagc cctaccgaac cgaagacccg
9961 gcgcgccggc gtccggcact ccggacaggt cgccgccgca tcgcgctcgg agatcgcgca
10021 ggtgagatcg aagggaccgc agtgggaaca acggaatgtg taggtcggca tggtcacccc
10081 tttccattca cccgaaatat atccagatga aaataaaagt caagggtgaa cgcccgcgcc
10141 gggcgcgggt ggttacgaag tcaaattcga ggtggtgtcg acgagttcgc gcaactggtc
10201 gacgtccagc ccgcacgatt cgaaagccgc acactcggcc tcggctaccg cgtccaccag
10261 gcggttgcgc agcgtccgcc cccgcggggt caacgccacg agcacccgcc ggcggtcatg
10321 cagatcggca gtccggtaga tgatcccttg cgacacaagg cgatcgacgg ttctggtgag
10381 agtcggaccc gtgatcagcg tggcctgcgc gagatcgctc atgggcacgg tgcgccgtcc
10441 ggccaactcg tcgagcacgc gccagtcctc gatcttgagc gacgagtccg cgagcacgcg
10501 ttcgatcgcc gactccgcga ggtgggtcag acgcaccaac ggcagcagga ggttggggcg
10561 gcgctgctgt ccggcggcca tggtgggtgt ctcctcgtgt cgacggcggg gtctgttcag
10621 aatgaattat ttctgctaga aagctttgaa gcgcagacta cacgggatcg acggtgatct
10681 ggagtgcagg acggcgaggt tgagttccgc gtcgggctgg tgattccgct tcaagggcca
10741 gcgggaatct tcgcgccttc gtgcgaggcc gtggccgagc tcgcggccaa ggaggtcaac
10801 gaccgaggcg gcctgcaggg ccgcaaggtc accatcgagg tgctcgacgg cggagcgccc
10861 ggcgacgacg tcgcccggac cgtcgccgac cggttgcgag gtcacggtct cgacgccgtg
10921 accggctggc acatctcggc cgtgcgcaac cgcatctccc cggtggtccg cgaccgcatc
10981 ccgtacgtct acacctcgtt gtacgagggc ggtgaacgca caccgggcgt gttctgcaca
11041 ggcgagacac cgcagatcca gatcgcgccc gcgctcgcct ggctgcgcga ccacttcggc
11101 atccggtcct ggtgcctggt cggcgatgac tacatctggc cgcgccgttc cgccgcggcc
11161 gcccgcgcgt actgccgaga tctggacctc gagctcagac gggagatcta cgtcccgtac
11221 ggcaccgacg atttccgcgc acctgtccgc aaggccatcg cgtcgggggc gcaggccgtg
11281 ttgatgctgc tcgtcggcca ggacgccgtg ttgttcaacc gcgagttcgc gcgcgccggc
11341 gggcacgacc gcatggcgcg gttcagcccg ctgatggagg agaacatgct gctggccagc
11401 ggcgccggct ccaccgaaaa cctctatgtc gccgcggcgt acttcagttc actggccacc
11461 gcgggtgcca tggacctgat gggcagctac gtcgcccgct acggcgccga cgcgccaccg
11521 ctcaacgcga tggccgaatc ctgttacgag ggtctgcttg cgctcgaggc catcttccag
11581 cgggcccact cccccgagat accggacctg atggcatccg cgcacgatgt cgggttcgac
11641 gggccccggg gcccgatgtg tatgcgcgac agtcaattcg accagcaggt ctacatcgcc
11701 agcgccgacg gctacgactt cgacatcctc gacacgctgg cgacgctcga cgcttgagac
11761 cgcgtcactt ctttatctag atttaaagat ctggtaccg
//
