| 质粒名称: | TERT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 156078 |
| 存储实验室: | Eugene Yeo |
|
载体骨架基本信息 |
|
| 载体名称: | E2.pEF5-DEST-FL-V5-MCP |
| 载体出品公司: | Clontech |
| 空载体大小: | 7357 bp |
| 完整质粒大小: | 9728 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | TERT、BC172541 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3363 bp |
| 基因突变: | 无 |
| 启动子: | EF-1a |
| 标签/融合蛋白: | V5-MCP (C terminal on backbone) |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | TAATACGACTCACTATAGGG |
| 3’测序引物 | gctcgagtctagagggc |
|
文献引用方法 |
|
| 材料和方法部分: | TERT was a gift from Eugene Yeo (Addgene plasmid # 156078 ; http://n2t.net/addgene:156078 ; RRID:Addgene_156078) |
| 参考文献部分: | Large-scale tethered function assays identify factors that regulate mRNA stability and translation. Luo EC, Nathanson JL, Tan FE, Schwartz JL, Schmok JC, Shankar A, Markmiller S, Yee BA, Sathe S, Pratt GA, Scaletta DB, Ha Y, Hill DE, Aigner S, Yeo GW. Nat Struct Mol Biol. 2020 Oct;27(10):989-1000. doi: 10.1038/s41594-020-0477-6. Epub 2020 Aug 17. 10.1038/s41594-020-0477-6 PubMed 32807991 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS TERT 9728 bp ds-DNA circular SYN
10-NOV-2022
DEFINITION For use in RBP tethering screen.
ACCESSION .
VERSION .
KEYWORDS TERT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9728)
TITLE Yeo Lab plasmids
REFERENCE 2 (bases 1 to 9728)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 10, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9728
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1179
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human
elongation
factor EF-1-alpha"
intron 232..1170
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of
human
EF-1-alpha"
primer_bind 1127..1147
/label=EF1a-F
/note="Human elongation factor-1a promoter,
forward primer"
primer_bind 1196..1215
/label=T7
/note="T7 promoter, forward primer"
promoter 1196..1214
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA
polymerase"
protein_bind 1293..1317
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R)
BP reaction"
CDS complement(4336..4347)
/codon_start=1
/product="WELQut protease recognition and
cleavage site"
/label=WELQut site
/translation="WELQ"
protein_bind complement(4685..4709)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R)
BP reaction"
CDS 4834..4875
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(5330..5347)
/label=BGH-rev
/note="Bovine growth hormone terminator,
reverse primer.
Also called BGH reverse"
polyA_signal 5336..5560
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation
signal"
primer_bind complement(5693..5712)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 5844..5891
/label=FRT
/bound_moiety="FLP recombinase from the
Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in
the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 5899..6921
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase
from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 7051..7172
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7088..7107)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7142..7161
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7221..7237)
/label=M13 rev
/note="common sequencing primer, one of
multiple similar
variants"
primer_bind complement(7221..7237)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7234..7256)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7245..7261
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac
operator to
inhibit transcription in E. coli. This
inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside
(IPTG)."
promoter complement(7269..7299)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7314..7335
/label=CAP binding site
/bound_moiety="E. coli catabolite activator
protein"
/note="CAP binding activates transcription in
the presence
of cAMP."
primer_bind complement(7452..7469)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7623..8211)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC
origin of
replication"
primer_bind complement(7703..7722)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8382..9242)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin,
carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9005..9024
/label=Amp-R
/note="Ampicillin resistance gene, reverse
primer"
promoter complement(9243..9347)
/gene="bla"
/label=AmpR promoter
primer_bind complement(9422..9441)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast
selectable
marker"
ORIGIN
1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
gaagttgggg
61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
ctgggaaagt
121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
tataagtgca
181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
ggtaagtgcc
241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
gccttgaatt
301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
aagtgggtgg
361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
tgaggcctgg
421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
tctcgctgct
481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
ttttttctgg
541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
ttttggggcc
601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
gggcctgcga
661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
tctggtgcct
721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
gtcggcacca
781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
aaaatggagg
841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
ggcctttccg
901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
gcacctcgat
961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
ttatgcgatg
1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
cttgatgtaa
1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
gcctcagaca
1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt
ggtactaata
1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc
tcggatccac
1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa
gcaggctgcc
1321 accatgccgc gcgctccccg ctgccgagcc gtgcgctccc tgctgcgcag
ccactaccgc
1381 gaggtgctgc cgctggccac gttcgtgcgg cgcctggggc cccagggctg
gcggctggtg
1441 cagcgcgggg acccggcggc tttccgcgcg ctggtggccc agtgcctggt
gtgcgtgccc
1501 tgggacgcac ggccgccccc cgccgccccc tccttccgcc aggtgtcctg
cctgaaggag
1561 ctggtggccc gagtgctgca gaggctgtgc gagcgcggcg cgaagaacgt
gctggccttc
1621 ggcttcgcgc tgctggacgg ggcccgcggg ggcccccccg aggccttcac
caccagcgtg
1681 cgcagctacc tgcccaacac ggtgaccgac gcactgcggg ggagcggggc
gtgggggctg
1741 ctgctgcgcc gcgtgggcga cgacgtgctg gttcacctgc tggcacgctg
cgcgctcttt
1801 gtgctggtgg ctcccagctg cgcctaccag gtgtgcgggc cgccgctgta
ccagctcggc
1861 gctgccactc aggcccggcc cccgccacac gctagtggac cccgaaggcg
tctgggatgc
1921 gaacgggcct ggaaccatag cgtcagggag gccggggtcc ccctgggcct
gccagccccg
1981 ggtgcgagga ggcgcggggg cagtgccagc cgaagtctgc cgttgcccaa
gaggcccagg
2041 cgtggcgctg cccctgagcc ggagcggacg cccgttgggc aggggtcctg
ggcccacccg
2101 ggcaggacgc gtggaccgag tgaccgtggt ttctgtgtgg tgtcacctgc
cagacccgcc
2161 gaagaagcca cctctttgga gggtgcgctc tctggcacgc gccactccca
cccatccgtg
2221 ggccgccagc accacgcggg ccccccatcc acatcgcggc caccacgtcc
ctgggacacg
2281 ccttgtcccc cggtgtacgc cgagaccaag cacttcctct actcctcagg
cgacaaggag
2341 cagctgcggc cctccttcct actcagctct ctgaggccca gcctgactgg
cgctcggagg
2401 ctcgtggaga ccatctttct gggttccagg ccctggatgc cagggactcc
ccgcaggttg
2461 ccccgcctgc cccagcgcta ctggcaaatg cggcccctgt ttctggagct
gcttgggaac
2521 cacgcgcagt gcccctacgg ggtgctcctc aagacgcact gcccgctgcg
agctgcggtc
2581 accccagcag ccggtgtctg tgcccgggag aagccccagg gctctgtggc
ggcccccgag
2641 gaggaggaca cagacccccg tcgcctggtg cagctgctcc gccagcacag
cagcccctgg
2701 caggtgtacg gcttcgtgcg ggcctgcctg cgccggctgg tgcccccagg
cctctggggc
2761 tccaggcaca acgaacgccg cttcctcagg aacaccaaga agttcatctc
cctggggaag
2821 catgccaagc tctcgctgca ggagctgacg tggaagatga gcgtgcggga
ctgcgcttgg
2881 ctgcgcagga gcccaggggt tggctgtgtt ccggccgcag agcaccgtct
gcgtgaggag
2941 atcctggcca agttcctgca ctggctgatg agtgtgtacg tcgtcgagct
gctcaggtct
3001 ttcttttatg tcacggagac cacgtttcaa aagaacaggc tctttttcta
ccggaagagt
3061 gtctggagca agttgcaaag cattggaatc agacagcact tgaagagggt
gcagctgcgg
3121 gagctgtcgg aagcagaggt caggcagcat cgggaagcca ggcccgccct
gctgacgtcc
3181 agactccgct tcatccccaa gcctgacggg ctgcggccga ttgtgaacat
ggactacgtc
3241 gtgggagcca gaacgttccg cagagaaaag agggccgagc gtctcacctc
gagggtgaag
3301 gcactgttca gcgtgctcaa ctacgagcgg gcgcggcgcc ccggcctcct
gggcgcctct
3361 gtgctgggcc tggacgatat ccacagggcc tggcgcacct tcgtgctgcg
tgtgcgggcc
3421 caggacccgc cgcctgagct gtactttgtc aaggacaggc tcacggaggt
catcgccagc
3481 atcatcaaac cccagaacac gtactgcgtg cgtcggtatg ccgtggtcca
gaaggccgcc
3541 catgggcacg tccgcaaggc cttcaagagc cacgtctcta ccttgacaga
cctccagccg
3601 tacatgcgac agttcgtggc tcacctgcag gagaccagcc cgctgaggga
tgccgtcgtc
3661 atcgagcaga gctcctccct gaatgaggcc agcagtggcc tcttcgacgt
cttcctacgc
3721 ttcatgtgcc accacgccgt gcgcatcagg ggcaagtcct acgtccagtg
ccaggggatc
3781 ccgcagggct ccatcctctc cacgctgctc tgcagcctgt gctacggcga
catggagaac
3841 aagctgtttg cggggattcg gcgggacggg ctgctcctgc gtttggtgga
tgatttcttg
3901 ttggtgacac ctcacctcac ccacgcgaaa accttcctca ggaccctggt
ccgaggtgtc
3961 cctgagtatg gctgcgtggt gaacttgcgg aagacagtgg tgaacttccc
tgtagaagac
4021 gaggccctgg gtggcacggc ttttgttcag atgccggccc acggcctatt
cccctggtgc
4081 ggcctgctgc tggatacccg gaccctggag gtgcagagcg actactccag
ctatgcccgg
4141 acctccatca gagccagtct caccttcaac cgcggcttca aggctgggag
gaacatgcgt
4201 cgcaaactct ttggggtctt gcggctgaag tgtcacagcc tgtttctgga
tttgcaggtg
4261 aacagcctcc agacggtgtg caccaacatc tacaagatcc tcctgctgca
ggcgtacagg
4321 tttcacgcat gtgtgctgca gctcccattt catcagcaag tttggaagaa
ccccacattt
4381 ttcctgcgcg tcatctctga cacggcctcc ctctgctact ccatcctgaa
agccaagaac
4441 gcagggatgt cgctgggggc caagggcgcc gccggccctc tgccctccga
ggccgtgcag
4501 tggctgtgcc accaagcatt cctgctcaag ctgactcgac accgtgtcac
ctacgtgcca
4561 ctcctggggt cactcaggac agcccagacg cagctgagtc ggaagctccc
ggggacgacg
4621 ctgactgccc tggaggccgc agccaacccg gcactgccct cagacttcaa
gaccatcctg
4681 gacgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
ccgctcgagt
4741 ctagagggcc cgcggttcga aaccagcgga ggcggcggaa gcgggggagg
aggcagcggc
4801 ggaggcggca gcgggggcgg cggaagcgct accggtaagc ctatccctaa
ccctctcctc
4861 ggtctcgatt ctacgcgtac cggtatggct tctaacttta ctcagttcgt
tctcgtcgac
4921 aatggcggaa ctggcgacgt gactgtcgcc ccaagcaact tcgctaacgg
ggtcgctgaa
4981 tggatcagct ctaactcgcg atcacaggct tacaaagtaa cctgtagcgt
tcgtcagagc
5041 tctgcgcaga atcgcaaata caccatcaaa gtcgaggtgc ctaaagtggc
aacccagact
5101 gttggtggtg aagagcttcc tgtagccgga tggagatctt acttaaatat
ggaactaacc
5161 attccaattt tcgccacgaa ttccgactgc gagcttattg ttaaggcaat
gcaaggtctc
5221 ctaaaagatg gaaacccgat tccctcggcc atcgcggcca actccggcat
ctacggaggt
5281 ggaggtggat ctcgtacggg ttagtaatga gtttaaaccc gctgatcagc
ctcgactgtg
5341 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
gaccctggaa
5401 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
ttgtctgagt
5461 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
ggattgggaa
5521 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
ggaaagaacc
5581 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
cgcggcgggt
5641 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
cgctcctttc
5701 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
tctaaatcgg
5761 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
aaaacttgat
5821 tagggtgatg gttcacgtac ctagaagttc ctattccgaa gttcctattc
tctagaaagt
5881 ataggaactt ccttggccaa aaagcctgaa ctcaccgcga cgtctgtcga
gaagtttctg
5941 atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
agaatctcgt
6001 gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
ctgcgccgat
6061 ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
cccgattccg
6121 gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
ccgccgtgca
6181 cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
gcagccggtc
6241 gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
gttcggccca
6301 ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
cgcgattgct
6361 gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
gtccgtcgcg
6421 caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
gcacctcgtg
6481 cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
agcggtcatt
6541 gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
cttcttctgg
6601 aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
gcatccggag
6661 cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
ccaactctat
6721 cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
atgcgacgca
6781 atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
aagcgcggcc
6841 gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
ccccagcact
6901 cgtccgaggg caaaggaata gcacgtacta cgagatttcg attccaccgc
cgccttctat
6961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
ccagcgcggg
7021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
taatggttac
7081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
gcattctagt
7141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
gacctctagc
7201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
tccgctcaca
7261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
ctaatgagtg
7321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
aaacctgtcg
7381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
tattgggcgc
7441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
gcgagcggta
7501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
cgcaggaaag
7561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
gttgctggcg
7621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
aagtcagagg
7681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
ctccctcgtg
7741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
cccttcggga
7801 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
ggtcgttcgc
7861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
cttatccggt
7921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
agcagccact
7981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
gaagtggtgg
8041 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
gaagccagtt
8101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
tggtagcggt
8161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
agaagatcct
8221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
agggattttg
8281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
atgaagtttt
8341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
cttaatcagt
8401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
actccccgtc
8461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
aatgataccg
8521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
cggaagggcc
8581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
ttgttgccgg
8641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
cattgctaca
8701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
ttcccaacga
8761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
cttcggtcct
8821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
ggcagcactg
8881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
tgagtactca
8941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
ggcgtcaata
9001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
aaaacgttct
9061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
gtaacccact
9121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
gtgagcaaaa
9181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
ttgaatactc
9241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
catgagcgga
9301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
atttccccga
9361 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
tgcactctca
9421 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
tgtgtgttgg
9481 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
ttgaccgaca
9541 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
gtacgggcca
9601 gatatacgcg ttgacattga ttattgacta ggcttttgca aaaagctttg
caaagatgga
9661 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc
ttgaaaggag
9721 tgggaatt
//
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