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pTRE2pur载体质粒基本信息
pTRE2pur质粒图谱载体图谱和pTRE2pur载体序列质粒序列多克隆位点信息
pTRE2pur质粒载体简介
pTRE2pur质粒序列载体序列
LOCUS pTRE2pur 5108 bp ds-DNA circular SYN 19-OCT-2017
DEFINITION Expression vector with a Tet-responsive promoter and a puromycin
resistance marker.
ACCESSION .
VERSION .
KEYWORDS pTRE2pur
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5108)
AUTHORS Clontech (TaKaRa)
TITLE Direct Submission
JOURNAL Exported Saturday, November 30, 2019 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5108
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
protein_bind 15..285
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 318..356
/note="minimal CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 471..532
/note="MCS"
/note="multiple cloning site"
intron 554..1126
/note="beta-globin intron"
/note="intron from rabbit beta-globin gene"
polyA_site 1323..1378
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
rep_origin complement(1947..2535)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2706..3566)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3567..3671)
/gene="bla"
/note="AmpR promoter"
promoter 3776..4105
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 3956..4091
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 4198..4797
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 5027..5108
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
ORIGIN
1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc
541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca ctcgagtgtg
3781 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
3841 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3901 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
3961 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
4021 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
4081 ttttggaggc ctaggctttt gcaaaaagct tgcatgcctg caggtcggcc gccacgaccg
4141 gtgccgccac catcccctga cccacgcccc tgacccctca caaggagacg accttccatg
4201 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc
4261 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc
4321 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
4381 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
4441 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
4501 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
4561 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
4621 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
4681 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
4741 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
4801 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgg ctccgaccga
4861 agccacccgg ggcggccccg ccgaccccgc acccgccccc gaggcccacc gactctagag
4921 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
4981 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
5041 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
5101 ttcactgc
//
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