| 质粒名称: | pMx-puro-MGT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 111809 |
| 存储实验室: | Li Qian |
|
载体骨架基本信息 |
|
| 载体名称: | pMx-puromycin |
| 载体出品公司: | Cell biolabs |
| 空载体大小: | 5900bp |
| 完整质粒大小: | 9974bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Mef2c, Tbx5 and Gata4 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 4347bp |
| 启动子: | viral LTR |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI (not destroyed) |
| 3’克隆位点: | XhoI (destroyed during cloning) |
| 5’测序引物 | GACGGCATCGCAGCTTGGATACAC |
|
文献引用方法 |
|
| 材料和方法部分: |
pMx-puro-MGT was a gift from Li Qian (Addgene plasmid # 111809 ; http://n2t.net/addgene:111809 ; RRID:Addgene_111809)
|
| 参考文献部分: | Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency and quality of induced cardiac myocyte reprogramming. Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian L. Circ Res. 2015 Jan 16;116(2):237-44. doi: 10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21. 10.1161/CIRCRESAHA.116.305547 PubMed 25416133 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMx-puro-MGT 9953 bp ds-DNA circular SYN 23-FEB-2024
DEFINITION Retroviral expression vector for direct cardiac reprogramming.
ACCESSION .
VERSION .
KEYWORDS pMx-puro-MGT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9953)
AUTHORS Wang L, Liu Z, Yin C, Asfour H, Chen O, Li Y, Bursac N, Liu J, Qian
L
TITLE Stoichiometry of Gata4, Mef2c, and Tbx5 influences the efficiency
and quality of induced cardiac myocyte reprogramming.
JOURNAL Circ Res. 2015 Jan 16;116(2):237-44. doi:
10.1161/CIRCRESAHA.116.305547. Epub 2014 Nov 21.
PUBMED 25416133
REFERENCE 2 (bases 1 to 9953)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 23, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9953
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..594
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 657..1014
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1073..1489
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1419..1441
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1457..1473
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 1499..1873
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind 1811..1827
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS 3216..3272
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 4617..4670
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
primer_bind complement(6267..6287)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6272..6601
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6452..6587
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6514..6533
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6611..7210
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6611..6630)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7107..7127
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 7437..7906
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(8036..8053)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8207..8795)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8287..8306)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8959..9819)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9582..9601
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9820..9924)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
1861 atcctctaga ctgccggatc tagctagtta attaaggatc cgccatgggg agaaaaaaga
1921 ttcagattac gaggataatg gatgagcgta acagacaggt gacttttacg aagaggaaat
1981 ttggattgat gaagaaggct tatgagctga gcgtgctgtg cgactgtgag attgcactga
2041 tcatcttcaa cagcaccaac aagctgttcc agtacgccag cactgacatg gataaggtgt
2101 tgctcaagta caccgagtac aacgagccgc acgagagccg gacaaactca gacattgtgg
2161 aggcattgaa caagaaagaa aacaaaggct ctgaaagccc cgatcctgac tcctcttatg
2221 cactcacccc acgcactgaa gaaaaataca aaaaaattaa tgaagaattt gataatatga
2281 tcaagagtca taaaattcct gctgttccac ctcccagctt tgagatgcca gttaccatcc
2341 cagtgtccag ccataacagt ttggtgtaca gcaatcctgt cagcacactg ggaaacccca
2401 atcttctgcc actggcccac ccgtctctgc agaggaatag tatgtctcct ggtgtaacac
2461 atagacctcc aagtgcaggt aacacaggcg gtctgatggg cggagatctg acatccggtg
2521 caggcaccag cgcagggaat ggatacggca acccccggaa ctcaccaggc ctgctggtct
2581 cacctggtaa cctgaacaag aatatacaag ccaaatctcc tccccctatg aatctaggaa
2641 tgaataatcg taagccagat ctccgcgttc ttatcccacc tggcagcaag aacacgatgc
2701 catcagtgaa tcaaaggata aataactccc agtcggctca gtcattggct accccggtgg
2761 tttccgtagc aactcctact ttaccaggac aaggaatggg aggatatcca tcagccattt
2821 caacaacata tggtactgag tactctctga gtagcgcaga tctgtcatct ctgtctggct
2881 tcaacactgc cagtgcgctc cacctcggct ctgtaactgg ctggcagcag cagcacctac
2941 ataacatgcc gccatctgcc ctcagtcagt tgggagaccg taccaccacc ccttcgagat
3001 acccacaaca caccacgcgc cacgaggcgg ggaggtctcc tgttgacagc ttgagcagct
3061 gtagcagttc ctacgatggg agcgaccgag aggatcaccg gaacgaattc cactccccca
3121 ttggactcac cagaccttcg ccggacgaaa gggaaagtcc ttcagtcaag cgcatgcgac
3181 tctctgaagg atgggcaaca gctagcggca gcggcgccac gaacttctct ctgttaaagc
3241 aagcaggaga tgttgaagaa aaccccgggc ctgcatgcat gtaccaaagc ctggccatgg
3301 ccgccaacca cggccccccg cccggcgcct acgaagcagg tggccctggc gccttcatgc
3361 acagcgcggg cgccgcgtcc tcgcccgtct acgtgcccac tccgcgggtg ccgtcctctg
3421 tgctgggcct gtcctacctg cagggcggtg gcagtgccgc tgcagctgga accacctcgg
3481 gtggcagctc cggggccggc ccgtcgggtg cagggcctgg gacccagcag ggtagccctg
3541 gctggagcca agctggagcc gagggagccg cctacacccc gccgcccgtg tccccgcgct
3601 tctctttccc ggggactact gggtccctgg cggccgctgc cgccgctgcc gcagcccggg
3661 aagctgcagc ctacggcagt ggcggcgggg cggcgggcgc tggtctggct ggccgagagc
3721 agtacgggcg tccgggcttc gccggctcct actccagccc ctacccagcc tacatggccg
3781 acgtgggagc atcctgggcc gcagccgctg ccgcctctgc cggccccttc gacagcccag
3841 tcctgcacag cctgcctgga cgggccaacc ctggaagaca ccccaatctc gatatgtttg
3901 atgacttctc agaaggcaga gagtgtgtca attgtggggc catgtccacc ccactctgga
3961 ggcgagatgg gacgggacac tacctgtgca atgcctgtgg cctctatcac aagatgaacg
4021 gcatcaaccg gcccctcatt aagcctcagc gccgcctgtc cgcttcccgc cgggtaggcc
4081 tctcctgtgc caactgccag actaccacca ccacgctgtg gcgtcgtaat gccgagggtg
4141 agcctgtatg taatgcctgc ggcctctaca tgaagctcca tggggttccc aggcctcttg
4201 caatgcggaa ggaggggatt caaaccagaa aacggaagcc caagaacctg aataaatcta
4261 agacgccagc aggtcctgct ggtgagaccc tccctccctc cagtggtgcc tccagcggta
4321 actccagcaa tgccactagc agcagcagca gcagtgaaga gatgcgcccc atcaagacag
4381 agcccgggct gtcatctcac tatgggcaca gcagctccat gtcccagaca ttcagtactg
4441 tgtccggcca cgggccctcc atccatccag tgctgtctgc tctgaagctg tccccacaag
4501 gctatgcatc tcctgtcact cagacatcgc aggccagctc caagcaggac tcttggaaca
4561 gcctggtcct ggctgacagt catggggaca taatcaccgc ggcatgcggc tccggcgagg
4621 gcaggggaag tcttctaaca tgcggggacg tggaggaaaa tcccggccca ctcgagatgg
4681 ccgatacaga tgagggcttt ggcctggcgc gcacgcctct ggagcctgat tccaaagaca
4741 ggtcttgcga ttcgaaacct gagagtgctc tgggggctcc cagcaagtct ccatcatccc
4801 cgcaggctgc cttcacccag cagggcatgg aaggaatcaa ggtgtttctt catgaacgtg
4861 aactgtggct gaagttccac gaagtgggca cagagatgat catcaccaag gcagggagga
4921 gaatgtttcc tagttacaaa gtgaaggtga ctggccttaa tcccaaaacg aagtatattc
4981 ttctcatgga tattgttccc gcagacgacc acagatataa atttgctgat aacaaatggt
5041 ccgtaactgg caaagcagag cctgccatgc cggggcgcct ttacgtgcac ccggactccc
5101 cagcaaccgg agcccactgg atgcgacaac ttgtctcctt ccagaagctc aaactcacca
5161 acaaccacct ggacccgttt ggacacatta tcctgaactc catgcacaaa taccagcccc
5221 gattacacat cgtgaaagca gacgaaaata atgggttcgg ttcaaagaac actgcgtttt
5281 gcacccacgt cttcccggag acagctttta tcgctgtgac ttcgtaccag aatcacaaga
5341 tcacacagct gaaaattgag aacaacccct tcgccaaagg ctttcggggc agtgatgacc
5401 tggagttaca caggatgtct cggatgcaaa gtaaagagta tcctgtggtt cccaggagca
5461 cagtgaggca caaagtcacc tccaaccaca gccccttcag cagcgagacc cgagctctct
5521 ccacctcatc caatttaggg tcccagtacc agtgtgagaa tggtgtctct ggcccctccc
5581 aggaccttct gcccccacct aacccatacc cactggccca ggagcacagc caaatttacc
5641 actgtaccaa gaggaaagat gaggaatgtt ccagcacgga gcacccctat aagaagccgt
5701 acatggagac atcccccagc gaggaagaca ccttctatcg ctcgggctac ccccagcagc
5761 agggcctgag tacctcttac aggacagagt cggcccagcg gcaggcctgc atgtatgcca
5821 gctccgctcc ccccagcgag cccgtgccta gcctggagga catcagctgt aacacatggc
5881 ccagcatgcc ctcctatagc agctgtaccg tcaccaccgt gcagcccatg gaccgtcttc
5941 cctaccagca cttctccgct catttcacct cggggcccct ggtccctcgg ttggctggca
6001 tggccaacca tggttctccc cagctcggcg aagggatgtt tcagcaccag acctcagtgg
6061 cccatcagcc tgtggtcagg cagtgcgggc ctcagactgg ccttcagtct ccgggcggcc
6121 tccagccccc agagtttctc tacactcacg gcgtgcccag gaccctgtcc ccccatcagt
6181 atcactcggt acacggcgtc ggcatggtgc cagagtggag tgagaatagc taagtcgagc
6241 ggccgccagc acagtggtcg acctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
6301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
6361 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
6421 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
6481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
6541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
6601 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
6661 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
6721 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
6781 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
6841 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
6901 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
6961 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
7021 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
7081 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
7141 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
7201 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
7261 tgcatcgtcg acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
7321 agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa
7381 aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga
7441 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
7501 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
7561 ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa
7621 ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact
7681 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa
7741 gagcccacaa cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc
7801 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
7861 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
7921 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
7981 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
8041 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
8101 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
8161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
8221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
8281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
8341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
8401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
8461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
8521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
8581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
8641 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
8701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
8761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
8821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
8881 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
8941 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
9001 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
9061 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
9121 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
9181 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
9241 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
9301 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
9361 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
9421 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
9481 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
9541 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
9601 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
9661 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
9721 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
9781 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
9841 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
9901 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cat
//
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