| 质粒名称: | pAC-LYC |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 53270 |
| 存储实验室: | Francis X Cunningham Jr |
|
载体骨架基本信息 |
|
| 载体名称: | pAC-EHER |
| 载体出品公司: | Francis X. Cunningham, Jr. |
| 空载体大小: | 13581bp |
| 完整质粒大小: | 9476 bp |
| 载体类型: | low copy number bacterial cloning vector |
| 载体抗性: | Chloramphenicol |
| 生长温度: | 30 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | crtE, crtI, crtB |
| 物种来源: | Erwinia herbicola Eho10 |
| GenBank编号 | DM87280.1 |
| 启动子: | endogenous promoters |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | Restriction Enzyme |
| 5’克隆位点: | NA (unknown if destroyed) |
| 3’克隆位点: | NA (unknown if destroyed) |
| 5’测序引物 | 无 |
| 3’测序引物 | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pAC-LYC was a gift from Francis X Cunningham Jr (Addgene plasmid # 53270 ; http://n2t.net/addgene:53270 ; RRID:Addgene_53270) |
| 参考文献部分: | Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E. Plant Cell. 1994 Aug;6(8):1107-21. 10.1105/tpc.6.8.1107 PubMed 7919981 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAC-LYC 9476 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway
gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and
thereby produces lycopene in Escherichia coli.
ACCESSION .
VERSION .
KEYWORDS pAC-LYC
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9476)
AUTHORS Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E
TITLE Molecular structure and enzymatic function of lycopene cyclase from
the cyanobacterium Synechococcus sp strain PCC7942.
JOURNAL Plant Cell. 1994 Aug;6(8):1107-21.
PUBMED 7919981
REFERENCE 2 (bases 1 to 9476)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9476
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 134..153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(220..322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(714..731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(848..1393)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(928..947)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 1505..1533
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(7164..7183)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(9036..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
3781 cccttattgc ctcgggaatg cgggctgggc tatttcaccc taccactggc tattcgctgc
3841 cgctggcggt ggcccttgcc gacgcgattg ccgacagccc gcggctgggc agcgttccgc
3901 tctatcagct cacccggcag tttgccgaac gccactggcg caggcaggga ttcttccgcc
3961 tgctgaaccg gatgcttttc ctggccgggc gcgaggagaa ccgctggcgg gtgatgcagc
4021 gcttttatgg gctgccggag cccaccgtag agcgctttta cgccggtcgg ctctctctct
4081 ttgataaggc ccgcattttg acgggcaagc caccggttcc gctgggcgaa gcctggcggg
4141 cggcgctgaa ccattttcct gacagacgag ataaaggatg aaaaaaaccg ttgtgattgg
4201 cgcaggcttt ggtggcctgg cgctggcgat tcgcctgcag gcggcaggga tcccaaccgt
4261 actgctggag cagcgggaca agcccggcgg tcgggcctac gtctggcatg accagggctt
4321 tacctttgac gccgggccga cggtgatcac cgatcctacc gcgcttgagg cgctgttcac
4381 cctggccggc aggcgcatgg aggattacgt caggctgctg ccggtaaaac ccttctaccg
4441 actctgctgg gagtccggga agaccctcga ctatgctaac gacagcgccg agcttgaggc
4501 gcagattacc cagttcaacc cccgcgacgt cgagggctac cggcgctttc tggcttactc
4561 ccaggcggta ttccaggagg gatatttgcg cctcggcagc gtgccgttcc tctcttttcg
4621 cgacatgctg cgcgccgggc cgcagctgct taagctccag gcgtggcaga gcgtctacca
4681 gtcggtttcg cgctttattg aggatgagca tctgcggcag gccttctcgt tccactccct
4741 gctggtaggc ggcaacccct tcaccacctc gtccatctac accctgatcc acgcccttga
4801 gcgggagtgg ggggtctggt tccctgaggg cggcaccggg gcgctggtga acggcatggt
4861 gaagctgttt accgatctgg gcggggagat cgaactcaac gcccgggtcg aagagctggt
4921 ggtggccgat aaccgcgtaa gccaggtccg gctggcggat ggtcggatct ttgacaccga
4981 cgccgtagcc tcgaacgctg acgtggtgaa cacctataaa aagctgctcg gccaccatcc
5041 ggtggggcag aagcgggcgg cagcgctgga gcgcaagagc atgagcaact cgctgtttgt
5101 gctctacttc ggcctgaacc agcctcattc ccagctggcg caccatacca tctgttttgg
5161 tccccgctac cgggagctga tcgacgagat ctttaccggc agcgcgctgg cggatgactt
5221 ctcgctctac ctgcactcgc cctgcgtgac cgatccctcg ctcgcgcctc ccggctgcgc
5281 cagcttctac gtgctggccc cggtgccgca tcttggcaac gcgccgctgg actgggcgca
5341 ggaggggccg aagctgcgcg accgcatctt tgactacctt gaagagcgct atatgcccgg
5401 cctgcgtagc cagctggtga cccagcggat ctttaccccg gcagacttcc acgacacgct
5461 ggatgcgcat ctgggatcgg ccttctccat cgagccgctg ctgacccaaa gcgcctggtt
5521 ccgcccgcac aaccgcgaca gcgacattgc caacctctac ctggtgggcg caggtactca
5581 ccctggggcg ggcattcctg gcgtagtggc ctcggcgaaa gccaccgcca gcctgatgat
5641 tgaggatctg caatgagcca accgccgctg cttgaccacg ccacgcagac catggccaac
5701 ggctcgaaaa gttttgccac cgctgcgaag ctgttcgacc cggccacccg ccgtagcgtg
5761 ctgatgctct acacctggtg ccgccactgc gatgacgtca ttgacgacca gacccacggc
5821 ttcgccagcg aggccgcggc ggaggaggag gccacccagc gcctggcccg gctgcgcacg
5881 ctgaccctgg cggcgtttga aggggccgag atgcaggatc cggccttcgc tgcctttcag
5941 gaggtggcgc tgacccacgg tattacgccc cgcatggcgc tcgatcacct cgacggcttt
6001 gcgatggacg tggctcagac ccgctatgtc acctttgagg atacgctgcg ctactgctat
6061 cacgtggcgg gcgtggtggg tctgatgatg gccagggtga tgggcgtgcg ggatgagcgg
6121 gtgctggatc gcgcctgcga tctggggctg gccttccagc tgacgaatat cgcccgggat
6181 attattgacg atgcggctat tgaccgctgc tatctgcccg ccgagtggct gcaggatgcc
6241 gggctgaccc cggagaacta tgccgcgcgg gagaatcggg ccgcgctggc gcgggtggcg
6301 gagcggctta ttgatgccgc agagccgtac tacatctcct cccaggccgg gctacacgat
6361 ctgccgccgc gctgcgcctg ggcgatcgcc accgcccgca gcgtctaccg ggagatcggt
6421 attaaggtaa aagcggcggg aggcagcgcc tgggatcgcc gccagcacac cagcaaaggt
6481 gaaaaaattg ccatgctgat ggcggcaccg gggcaggtta ttcgggcgaa gacgacgagg
6541 gtgacgccgc gtccggccgg tctttggcag cgtcccgttt aggcgggcgg ccatgacgtt
6601 cacgcaggat cgcctgtagg tcggcaggct tgcgggcgta aataaaaccg aaggagacgc
6661 agccctcccg gccgcgcacc gcgtggtgca ggcggtgggc gacgtagagc cgcttcaggt
6721 agccccggcg cgggatcccg cctcgccgtg ctgtccggtc tcaacctgat ccgccagaat
6781 cgagccaacg ggatcggcca gcacgaattc ggtatgcgga gagtgtttgg caaaccatgc
6841 ctgcaagcca cccagcgtgc cgccggagcc aacgcctact accacggcat caaccctgcc
6901 tgccagctgg tcgaacagct ccggtgcggt ggtggtggcg tgcgccagcg ggttggccgg
6961 gttagagaac tgatcaatat agtaagcacc cggcgtctcc tctgccaggc ggcgggcgta
7021 gtcctgatag tactccgggt ggccctttgt cacgtcggag cgggtcaggc gcacatcaac
7081 acccagcgca cgcaggtggt agatcctcta cgccggacgc atcgtggccg gcatcaccgg
7141 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
7201 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
7261 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
7321 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
7381 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
7441 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
7501 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
7561 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
7621 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
7681 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
7741 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
7801 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
7861 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
7921 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
7981 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
8041 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
8101 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
8161 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
8221 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
8281 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
8341 gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct gctgaagttg cccgcaacag
8401 agagtggaac caaccggtga taccacgata ctatgactga gagtcaacgc catgagcggc
8461 ctcatttctt attctgagtt acaacagtcc gcaccgctgt ccggtagctc cttccggtgg
8521 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
8581 ggacaggtgc cggcagcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
8641 ccgaaacaag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
8701 ccctgtggaa cacctacatc tgtattaacg aagcgctaac cgtttttatc aggctctggg
8761 aggcagaata aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact
8821 ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa
8881 accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga
8941 atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca
9001 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
9061 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga
9121 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
9181 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
9241 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
9301 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
9361 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
9421 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacg
//
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