| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pVL1393 |
| 质粒类型: | 昆虫表达载体；杆状病毒载体 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Polyhedrin |
| 载体大小: | 9632 bp |
| 5' 测序引物及序列: | Polyhedrin Forward：5' d(AAA TGA TAA CCA TCT CGC) 3' |
| 3' 测序引物及序列: | Polyhedrin Reverse：5' d(GTC CAA GTT TCC CTG) 3' |
| 载体标签: | 无标签 |
| 载体抗性: | 氨苄青霉素 |
| 真核筛选标记: | 无 |
| 克隆菌株: | 推荐E.coli TOP10 |
| 宿主细胞（系）: | Sf9、Sf21、High Five细胞系 |
| 备注: | 相关载体有pAC360, pVL1392 |
| 产品目录号: | 12249-025 |
| 瞬表达/稳表达: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 杆状病毒 |
买家导航
pVL1393载体质粒基本信息
pVL1393质粒图谱载体图谱和pVL1393载体序列质粒序列多克隆位点信息
pVL1393质粒载体简介
pVL1393载体和pVL1392载体简介
pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.
Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.
These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.
载体特征
• Multiple cloning site polylinker, positioned in opposite orientations
• Recombination sequences for insertion into the baculovirus genome
• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein
Features for growth and maintenance in E. coli:
• Ampicillin gene for selection
• pMB1 origin of replication from pUC19 for high copy number growth
载体复制
The TOP10 cells are provided as a stab.
TOP10 is provided for growth and maintenance of this plasmid. TOP10 is a recombination-negative strain designed for stable replication of high copy number plasmids. This strain is provided as a convenience for those who do not have access to other E. coli strains. Many E. coli strains are suitable for the growth of this vector including DH5α, DH10, and INVαF'. In general, it is best to grow vectors containing inserts in E. coli strains which are recombination-deficient (recA-).
Genotype of TOP10
F-, mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG
pVL1393质粒序列载体序列
LOCUS pVL1393 9632 bp DNA SYN
DEFINITION pVL1393
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9632
/organism="pVL1393"
/mol_type="other DNA"
CDS 76..582
/label="ORF frame 1"
CDS complement(614..1600)
/label="ORF frame 2"
CDS complement(1868..2323)
/label="ORF frame 2"
CDS 2662..3294
/label="ORF frame 1"
misc_feature 2844..3990
/label="Ac_5_flank"
CDS complement(3332..3937)
/label="ORF frame 2"
promoter 3991..4091
/label="PH_promoter"
misc_feature 4017..4034
/label="polyhedrin_fwd_primer"
CDS 4250..4741
/label="ORF frame 2"
misc_feature complement(4288..4302)
/label="polyhedrinn_rev_primer"
misc_feature 4640..4741
/label="3_AcPH"
misc_feature complement(4657..4674)
/label="Baculovirs_rev_primer"
misc_feature 4755..6180
/label="Ac_3_flank"
CDS complement(4771..6024)
/label="ORF frame 3"
misc_feature complement(4853..5651)
/label="r_ORF1629"
CDS 6397..6987
/label="ORF frame 1"
promoter complement(7009..7027)
/label="M13_reverse_primer"
misc_feature complement(7026..7048)
/label="M13_pUC_rev_primer"
promoter complement(7062..7091)
/label="lac_promoter"
gene complement(8174..9034)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(8174..9034)
/label="ORF frame 2"
promoter complement(9076..9104)
/label="AmpR_promoter"
misc_feature complement(9263..9285)
/label="pGEX_3_primer"
misc_feature complement(9484..9627)
/label="lacZ_a"
misc_feature 9598..9620
/label="M13_pUC_fwd_primer"
promoter 9613..9629
/label="M13_forward20_primer"
ORIGIN
1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt
241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccgggta
4141 ccttctagaa ttccggagcg gccgctgcag atctgatcct ttcctgggac ccggcaagaa
4201 ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt
4261 cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag
4321 cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc
4381 cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga
4441 ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga
4501 gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga
4561 gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc
4621 catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct
4681 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta
4741 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt
4801 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta
4861 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc
4921 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc
4981 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag
5041 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc
5101 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact
5161 cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt
5221 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt
5281 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt
5341 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac
5401 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat
5461 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg
5521 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga
5581 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact
5641 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct
5701 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg
5761 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg
5821 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt
5881 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg
5941 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa
6001 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa
6061 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg ccgcacgccg
6121 ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg
6181 acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga
6241 acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc
6301 gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct attattgaat
6361 aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa tgctacgctg
6421 caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta taaaataatc
6481 aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa attgtatttg
6541 cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca ccagttaatg
6601 agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa caaccaagtg
6661 atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat taaaggcgag
6721 ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct caacgatttg
6781 cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata tttcgaagca
6841 cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc acttagcgtg
6901 cacgacggca cgttggagta ttttagtccg gaaaaaattc gacacacaac tatgcacgtt
6961 tcgtttgact ggtacgcggc gtgttaacat acaagttgct aacgtaatca tggtcatagc
7021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
7081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
7141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
9181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
9241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
9301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
9361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
9421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
9481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
9541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
9601 agtcacgacg ttgtaaaacg acggccagtg cc
//
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pVL1393质粒载体应用举例
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