| 产品公司： | ATCC |
|---|---|
| 载体名称: | pK19mobsacB、pK19mobsacB载体、pK19mobsacB质粒 |
| 产品编号： | 87098 |
| 生长条件： | 37°C 1 day(s) |
| 生物安全等级: | 1级 |
| 骨架载体名称： | |
| 原核抗性: | 卡那抗性 |
| 真核抗性: | SacB |
| DNA： | genomic |
| 建议宿主菌: | |
| 插入基因名： | |
| 插入基因源： | |
| 载体大小（kb）: | 5.7 |
| 插入基因大小： | 1.9 |
| 载体序列: | Genbank: LC257601.1 |
| 说明: | pK19mobsacB载体是谷氨酸棒杆菌基因敲除载体，Corynebacterium glutamicum gene Knockout vector。 |
| 参考文献: |
P446
1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145(1): 69-73.
|
买家导航
ATCC87098载体基本信息
ATCC87098载体图谱质粒图谱和多克隆位点信息
ATCC87098载体简介
ATCC87098载体序列
LOCUS pK19mobsacB 5719 bp ds-DNA circular SYN 18-SEP-2018
DEFINITION synthetic circular DNA
KEYWORDS pK19mobsacB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5719)
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..5719
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 346..1140
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="Kan"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter 1186..1631
/gene="sacR"
/note="sacB promoter"
/note="sacB promoter and control region"
CDS 1632..3053
/codon_start=1
/gene="Bacillus subtilis sacB"
/product="secreted levansucrase that renders bacterial
growth sensitive to sucrose"
/note="SacB"
/note="negative selection marker"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
oriT 3407..3516
/note="incP origin of transfer"
rep_origin 4485..5073
/direction=RIGHT
/note="oriV"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5487..5543
/note="MCS"
/note="pUC18/19 multiple cloning site"
ORIGIN
1 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
61 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
121 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
181 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
241 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
301 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
361 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
421 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
481 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg
541 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
601 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
661 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
721 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
781 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
841 gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc
901 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
961 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
1021 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
1081 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
1141 gcgggactct ggggttcgct agaggatcga tcctttttaa cccatcacat atacctgccg
1201 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
1261 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
1321 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
1381 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
1441 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
1501 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
1561 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
1621 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
1681 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
1741 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
1801 agcaaaaaaa tgaaaaatat caagtttctg aatttgattc gtccacaatt aaaaatatct
1861 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
1921 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
1981 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
2041 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
2101 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
2161 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
2221 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
2281 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
2341 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
2401 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
2461 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
2521 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
2581 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
2641 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
2701 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
2761 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
2821 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
2881 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
2941 cagacaaaca atcaacgttt gcgccgagct tcctgctgaa catcaaaggc aagaaaacat
3001 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaaaacgca
3061 aaagaaaatg ccgatgggta ccgagcgaaa tgaccgacca agcgacgccc aacctgccat
3121 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
3181 gggacgccct cgcggacgtg ctcatagtcc acgacgcccg tgattttgta gccctggccg
3241 acggccagca ggtaggccga caggctcatg ccggccgccg ccgccttttc ctcaatcgct
3301 cttcgttcgt ctggaaggca gtacaccttg ataggtgggc tgcccttcct ggttggcttg
3361 gtttcatcag ccatccgctt gccctcatct gttacgccgg cggtagccgg ccagcctcgc
3421 agagcaggat tcccgttgag caccgccagg tgcgaataag ggacagtgaa gaaggaacac
3481 ccgctcgcgg gtgggcctac ttcacctatc ctgcccggct gacgccgttg gatacaccaa
3541 ggaaagtcta cacgaaccct ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat
3601 accgaaaaaa tcgctataat gaccccgaag cagggttatg cagcggaaaa gcgctgcttc
3661 cctgctgttt tgtggaatat ctaccgactg gaaacaggca aatgcaggaa attactgaac
3721 tgaggggaca ggcgagagac gatgccaaag agctcctgaa aatctcgata actcaaaaaa
3781 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
3841 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
3901 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
3961 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
4021 cttctgtttc tatcagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
4081 cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc
4141 caaaagttgg cccagggctt cccggtatca acagggacac caggatttat ttattctgcg
4201 aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac
4261 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
4321 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaaaagg
4381 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
4441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
4501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
4621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
4681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
4741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
4801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
4861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
4921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
4981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
5041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
5101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
5161 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
5221 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
5281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
5341 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
5401 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
5461 ggaaacagct atgacatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat
5521 ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
5581 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
5641 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
5701 cgataagcta gcttcacgc
//
