| 出品公司: | Agilent |
|---|---|
| 载体名称: | pCFB |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 5292 bp |
| 5' 测序引物及序列: | CMV Forward： CGCAAATGGGCGGTAGGCGTG |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | DH5α 等通用菌株 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pCFB载体限制性酶切位点
pCFB, 5292 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 6 328, 381, 464, 650, 1153
5218
Acc65I 3 822, 2021, 2818
AccI 3 2142, 2232, 3178
AflII 3 202, 1440, 2373
AflIII 2 164, 3407
AgeI 1 2015
AhdI 6 862, 908, 1449, 2858, 2904
4295
Alw44I 4 1229, 3223, 3721, 4967
AlwNI 3 2494, 2569, 3818
ApaI 1 1413
ApoI 3 87, 1303, 2238
AvaI 8 785, 818, 1416, 2193, 2249
2748, 2781, 2814
BamHI 1 2244
BanII 5 939, 1413, 2751, 2764, 2935
BbeI 3 790, 1831, 2786
BbsI 1 5285
BbvCI 1 2624
BciVI 5 831, 2161, 2827, 3616, 5143
BglI 4 291, 413, 484, 4414
BglII 1 1853
BpmI 3 1974, 2321, 4385
Bpu10I 4 1723, 2508, 2583, 2624
BsaAI 4 545, 1991, 2175, 3159
BsaHI 10 328, 381, 464, 650, 790
1153, 1831, 2786, 4836, 5218
BsaI 9 869, 890, 957, 1589, 1977
2865, 2886, 2953, 4367
BsaWI 5 138, 2015, 3613, 3760, 4591
BseRI 5 893, 1243, 1736, 1775, 2889
BsiEI 8 1136, 1209, 2013, 2255, 3320
3744, 4667, 4816
BsiHKAI 6 1229, 2751, 3223, 3721, 4882
4967
BsmBI 6 1151, 1268, 1512, 1571, 1757
3056
Bsp120I 1 1413
BspHI 3 4127, 5135, 5240
BspLU11I 2 164, 3407
BsrBI 5 1474, 2252, 2277, 3338, 5139
BsrDI 2 4354, 4536
BsrGI 1 1716
BssHII 1 2734
BssSI 3 3580, 4964, 5271
Bst1107I 1 3178
BstAPI 3 2493, 2568, 3225
BstEII 1 1521
BstF5I 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
Bsu36I 1 1451
Cfr10I 2 2015, 4380
DraI 3 4164, 4183, 4875
DraIII 1 2048
DrdI 2 3096, 3509
DsaI 3 151, 567, 1106
EaeI 7 1002, 1136, 1543, 1564, 1843
2255, 4688
EarI 4 1509, 1739, 3285, 5089
Ecl136II 1 2751
Eco52I 2 1136, 2255
Eco57I 2 3934, 4982
Eco72I 1 2175
EcoNI 1 1822
EcoO109I 4 1652, 2099, 2670, 5275
EcoRI 1 2238
EcoRV 2 2479, 2554
EheI 3 790, 1831, 2786
FokI 10 777, 857, 1561, 1748, 2206
2853, 3082, 4280, 4461, 4748
FspI 1 4520
HaeII 5 790, 1831, 2786, 3281, 3651
HgaI 7 724, 1064, 1341, 3113, 3509
4087, 4837
HincII 2 2232, 4839
KasI 3 790, 1831, 2786
KpnI 3 822, 2021, 2818
MboII 10 1509, 1740, 2271, 3286, 4057
4148, 4903, 4981, 5090, 5286
MscI 3 1002, 1543, 1843
MslI 5 159, 568, 4548, 4707, 5066
MspA1I 9 151, 918, 2457, 2532, 2914
3117, 3747, 3992, 4933
MunI 2 11, 21
NarI 3 790, 1831, 2786
NcoI 1 567
NdeI 4 440, 1839, 1847, 3229
NheI 5 6, 16, 26, 197, 2368
NotI 1 2254
NsiI 1 774
NspI 4 164, 244, 3042, 3407
PleI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
Ppu10I 1 774
PpuMI 3 1652, 2099, 2670
PshAI 1 1190
Psp1406I 2 4525, 4898
PstI 3 1353, 1535, 4541
PvuI 2 1209, 4667
PvuII 2 2457, 2532
SacI 1 2751
SacII 1 151
SalI 1 2232
SapI 1 3284
ScaI 1 4778
SchI 10 617, 812, 1195, 1220, 2038
2283, 2808, 3307, 3778, 4295
SexAI 1 1649
SfcI 7 182, 1353, 1535, 2353, 3672
3863, 4541
SmaI 4 818, 1416, 2193, 2814
SmlI 8 202, 1440, 2249, 2373, 3513
3775, 4052, 4920
SnaBI 1 545
SpeI 1 1072
SphI 1 244
SrfI 1 1415
SspI 1 5102
StyI 5 567, 881, 1679, 2667, 2877
TaqI 9 1472, 1783, 1794, 2225, 2233
2250, 2294, 3507, 4951
TatI 7 424, 504, 537, 588, 1716
3213, 4778
TfiI 1 3382
Tsp45I 6 1457, 1666, 3062, 3157, 4557
4768
TspRI 10 1459, 2050, 3303, 3809, 3822
4093, 4242, 4347, 4694, 4721
Tth111I 4 808, 1648, 2804, 3151
VspI 1 4471
XbaI 2 2211, 2635
XhoI 1 2249
XhoII 8 1853, 2244, 4048, 4059, 4145
4157, 4925, 4942
XmaI 4 818, 1416, 2193, 2814
XmnI 1 4895
Enzymes that do NOT cut molecule:
AccIII AscI BclI BlnI
Bpu1102I BsaBI BsgI BsiWI
BsmI BspMI BstBI BstXI
ClaI Eco47III FseI HindIII
HpaI MluI NaeI NgoMIV
NruI PacI PmeI RsrII
SanDI SbfI SfiI SgfI
SgrAI StuI SwaI Van91I
XcmI
载体序列
pCFB, 5292 bp version 013001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG 51 AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT 101 CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG 151 CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA 201 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACGCATGCC 251 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG 301 CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 351 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT 401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG 451 TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG 501 CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA 551 TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG 601 GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 651 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA 701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG 751 GTGGGAGGTC TATATAAGCA GAGATGCATC CTCACTCGGG GCGCCAGTCC 801 TCCGATTGAC TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG 851 CAGTTGCATC CGACTTGTGG TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG 901 AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCATTTGGGG GCTCGTCCGG 951 GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC GGGAGGTAAG 1001 CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG 1051 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG 1101 CGGACCCGTG GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG 1151 GAGACGTCCC AGGGACTTCG GGGGCCGTTT TTGTGGCCCG ACCTGAGTCC 1201 AAAAATCCCG ATCGTTTTGG ACTCTTTGGT GCACCCCCCT TAGAGGAGGG 1251 ATATGTGGTT CTGGTAGGAG ACGAGAACCT AAAACAGTTC CCGCCTCCGT 1301 CTGAATTTTT GCTTTCGGTT TGGGACCGAA GCCGCGCCGC GCGTCTTGTC 1351 TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT CTGACTGTGT TTCTGTATTT 1401 GTCTGAAAAT ATGGGCCCGG GCCAGACTGT TACCACTCCC TTAAGTTTGA 1451 CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA 1501 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC 1551 CTTTAACGTC GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA 1601 CCCAGGTTAA GATCAAGGTC TTTTCACCTG GCCCGCATGG ACACCCAGAC 1651 CAGGTCCCCT ACATCGTGAC CTGGGAAGCC TTGGCTTTTG ACCCCCCTCC 1701 CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT CTTCCTCCAT 1751 CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC 1801 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCCCCA TATGGCCATA 1851 TGAGATCTTA TATGGGGCAC CCCCGCCCCT TGTAAACTTC CCTGACCCTG 1901 ACATGACAAG AGTTACTAAC AGCCCCTCTC TCCAAGCTCA CTTACAGGCT 1951 CTCTACTTAG TCCAGCACGA AGTCTGGAGA CCTCTGGCGG CACGTACCAA 2001 GAACAACTGG ACCGACCGGT GGTACCTCAC CCTTACCGAG TCGGCGACAC 2051 AGTGTGGGTC CGCCGACACC AGACTAAGAA CCTAGAACCT CGCTGGAAAG 2101 GACCTTACAC AGTCCTGCTG ACCACCCCCA CCGCCCTCAA AGTAGACGGC 2151 ATCGCAGCTT GGATACACGC CGCCCACGTG AAGGCTGCCG ACCCCGGGGG 2201 TGGACCATCC TCTAGACTGC CGGATCGAAT TGTCGACGAA TTCGGATCCT 2251 CGAGCGGCCG CGATCCGAGT TCTTCTGAGC GGGACTCTGG GGTTCGATAA 2301 AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC 2351 ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG 2401 GAAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TCAGGAACAG 2451 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC 2501 TGCCCCGGCT CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA 2551 CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG 2601 ATGGTCCCCA GATGCGGTCC AGCCCTCAGC AGTTTCTAGA GAACCATCAG 2651 ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG CCTTATTTGA 2701 ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC 2751 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG 2801 ATTGACTGAG TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT 2851 TGCATCCGAC TTGTGGTCTC GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG 2901 ATTGACTACC CGTCAGCGGG GGTCTTTCAT TTGGGGGCTC GTCCGGGATC 2951 GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA GGTAAGCTGG 3001 CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 3051 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA 3101 GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT 3151 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC 3201 ATCAGAGCAG ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC 3251 ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG 3301 CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 3351 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 3401 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 3451 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 3501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 3551 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 3601 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 3651 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 3701 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 3751 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 3801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 3851 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 3901 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 3951 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 4001 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 4051 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 4101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4151 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 4201 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 4251 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC 4301 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 4351 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC 4401 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 4451 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT 4501 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGGCAT 4551 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC 4601 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 4651 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT 4701 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 4751 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA 4801 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAACACGGGA 4851 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC 4901 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 4951 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT 5001 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 5051 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT 5101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 5151 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC 5201 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 5251 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AA
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载体质粒应用举例
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