| 出品公司: | -- |
|---|---|
| 载体名称: | pAAV-CMV-Rluc |
| 质粒类型: | 腺病毒载体；绿色海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | CMV |
| 载体大小: | 6824 bp |
| 5' 测序引物及序列: | pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 绿色海肾荧光素酶 |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS KC152484 6824 bp DNA circular SYN 03-JUN-2013
DEFINITION Expression vector pAAV-CMV-Rluc, complete sequence.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pAAV-CMV-Rluc|
AUTHORS Chen,Y., Cao,L., Luo,C., Ditzel,D.A., Peter,J. and Sprengel,R.
TITLE RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
JOURNAL Submitted (08-NOV-2012) Molecular Neurobiology, Max Planck
institute for medical research, Jahnstr. 29, Heidelberg, BW 69120,
Germany
FEATURES Location/Qualifiers
source 1..6824
/organism="Expression vector pAAV-CMV-Rluc"
/mol_type="other DNA"
/db_xref="taxon:1337867"
repeat_region 19..162
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
regulatory 429..955
/regulatory_class="promoter"
/note="CMV promoter"
intron 1069..1205
/note="chimeric intron"
misc_feature 1235..2167
/note="Renilla luciferase"
misc_feature 2171..2224
/note="2A peptide from Thosea asigna virus"
CDS 2240..2959
/note="fluorescent protein"
/codon_start=1
/transl_table=11
/product="Venus"
/protein_id="AGM53879.1"
/db_xref="GI:507593978"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKN
GIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYK"
ncRNA 2979..3575
/ncRNA_class="other"
/note="post-transcriptional regulatory element: WPRE"
misc_feature 3608..3818
/note="bovine growth hormone polyA signal: bGH pA"
repeat_region complement(3828..3971)
/note="AAV2 ITR"
/rpt_type=inverted
/rpt_type=terminal
gene 5160..5822
/gene="Bla"
CDS 5160..5822
/gene="Bla"
/note="ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AGM53880.1"
/db_xref="GI:507593979"
/translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKH
LTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELN
EAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAG
WFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIK
HW"
ORIGIN
1 gggggggggg ggggggggtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
61 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
121 gcgcgcagag agggagtggc caactccatc actaggggtt cctagatcta ggatcacgcg
181 ttctagacct tttgctcaca tggctcgaca gatcttcaat attggccatt agccatatta
241 ttcattggtt atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta
301 tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg ttggcattga
361 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
421 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
481 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
541 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
601 catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
661 gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc
721 gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac
781 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
841 aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt
901 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcact
961 agaagcttta ttgcggtagt ttatcacagt taaattgcta acgcagtcag tgcttctgac
1021 acaacagtct cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc
1081 aaggttacaa gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga
1141 ctcttgcgtt tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc
1201 acaggtgtcc actcccagtt cagaattcgg atccatgact tcgaaagttt atgatccaga
1261 acaaaggaaa cggatgataa ctggtccgca gtggtgggcc agatgtaaac aaatgaatgt
1321 tcttgattca tttattaatt attatgattc agaaaaacat gcagaaaatg ctgttatttt
1381 tttacatggt aacgcggcct cttcttattt atggcgacat gttgtgccac atattgagcc
1441 agtagcgcgg tgtattatac cagaccttat tggtatgggc aaatcaggca aatctggtaa
1501 tggttcttat aggttacttg atcattacaa atatcttact gcatggtttg aacttcttaa
1561 tttaccaaag aagatcattt ttgtcggcca tgattggggt gcttgtttgg catttcatta
1621 tagctatgag catcaagata agatcaaagc aatagttcac gctgaaagtg tagtagatgt
1681 gattgaatca tgggatgaat ggcctgatat tgaagaagat attgcgttga tcaaatctga
1741 agaaggagaa aaaatggttt tggagaataa cttcttcgtg gaaaccatgt tgccatcaaa
1801 aatcatgaga aagttagaac cagaagaatt tgcagcatat cttgaaccat tcaaagagaa
1861 aggtgaagtt cgtcgtccaa cattatcatg gcctcgtgaa atcccgttag taaaaggtgg
1921 taaacctgac gttgtacaaa ttgttaggaa ttataatgct tatctacgtg caagtgatga
1981 tttaccaaaa atgtttattg aatcggaccc aggattcttt tccaatgcta ttgttgaagg
2041 tgccaagaag tttcctaata ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga
2101 agatgcacct gatgaaatgg gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa
2161 tgaacaactc gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg
2221 ccctgcaccg ggatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
2281 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
2341 cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct
2401 gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg
2461 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
2521 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
2581 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
2641 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcac
2701 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
2761 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
2821 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga
2881 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
2941 ggacgagctg tacaagtaaa gcggccgcaa gcttatcgat aatcaacctc tggattacaa
3001 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
3061 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
3121 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
3181 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
3241 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
3301 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
3361 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
3421 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
3481 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
3541 tcggatctcc ctttgggccg cctccccgcc tgatcgatac cgtcgactag agctcgctga
3601 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
3661 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
3721 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
3781 ggggaggatt gggaagacaa tagcaggcat gctggggaga gatctgagga acccctagtg
3841 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
3901 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
3961 ggagtggcca accccccccc ccccccccct gcagcctggc gtaatagcga agaggcccgc
4021 accgatcgcc cttcccaaca gttgcgtagc ctgaatggcg aatggcgcga cgcgccctgt
4081 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4141 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4201 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4261 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
4321 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4381 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
4441 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
4501 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg
4561 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
4621 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
4681 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
4741 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
4801 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
4861 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
4921 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
4981 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5041 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5101 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5161 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5221 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5281 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5341 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5401 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5461 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
5521 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
5581 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
5641 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
5701 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
5761 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
5821 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
5881 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
5941 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6001 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6061 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6121 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
6181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6361 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
6421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
6601 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
6661 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
6721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
6781 cgcctctccc cgcgcgttgg ccgattcatt aatgcaggct gcag
//
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