质粒名称: | pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 |
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出品公司: | 爱迪基因 |
目录编号: | 103871 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pcDNA3.1 |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | 5428 bp |
完整质粒大小: | 9525 bp |
载体修饰: | 人细胞色素c氧化酶亚基VIII(human cytochrome C oxidase subunit VIII) |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | PspCas13b |
物种来源: | -- |
插入大小: | 2949 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII(N端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | AgeI(未破坏) |
3' 克隆位点: | NotI (未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 was a gift from Feng Zhang (Addgene plasmid # 103871 ; http://n2t.net/addgene:103871 ; RRID:Addgene_103871) |
参考文献部分: | RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703 |
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 9528 bp ds-DNA circular SYN 29-AUG-2018 DEFINITION dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV. ACCESSION . VERSION . KEYWORDS pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9528) AUTHORS Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F TITLE RNA editing with CRISPR-Cas13. JOURNAL Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. PUBMED 29070703 REFERENCE 2 (bases 1 to 9528) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 29, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9528 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 27..406 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 407..610 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 561..581 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 655..674 /label=T7 /note="T7 promoter, forward primer" promoter 655..673 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 3676..3702 /codon_start=1 /product="nuclear export signal from the HIV Rev protein (Fischer et al., 1995)" /label=NES /translation="LPPLERLTL" primer_bind complement(4911..4928) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4917..5141 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5187..5615 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5274..5293) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 5484..5505 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(5624..5644) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5629..5958 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5809..5944 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5871..5890 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 6025..6819 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(6079..6098) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 6689..6708 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 6993..7114 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7030..7049) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7084..7103 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7163..7179) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7163..7179) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7176..7198) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7187..7203 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7211..7241) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7256..7277 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7394..7411) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7565..8153) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7645..7664) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8324..9184) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8947..8966 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9185..9289) /gene="bla" /label=AmpR promoter primer_bind complement(9364..9383) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 661 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatgaac 721 atccccgctc tggtggaaaa ccagaagaag tactttggca cctacagcgt gatggccatg 781 ctgaacgctc agaccgtgct ggaccacatc cagaaggtgg ccgatattga gggcgagcag 841 aacgagaaca acgagaatct gtggtttcac cccgtgatga gccacctgta caacgccaag 901 aacggctacg acaagcagcc cgagaaaacc atgttcatca tcgagcggct gcagagctac 961 ttcccattcc tgaagatcat ggccgagaac cagagagagt acagcaacgg caagtacaag 1021 cagaaccgcg tggaagtgaa cagcaacgac atcttcgagg tgctgaagcg cgccttcggc 1081 gtgctgaaga tgtacaggga cctgaccaac gcatacaaga cctacgagga aaagctgaac 1141 gacggctgcg agttcctgac cagcacagag caacctctga gcggcatgat caacaactac 1201 tacacagtgg ccctgcggaa catgaacgag agatacggct acaagacaga ggacctggcc 1261 ttcatccagg acaagcggtt caagttcgtg aaggacgcct acggcaagaa aaagtcccaa 1321 gtgaataccg gattcttcct gagcctgcag gactacaacg gcgacacaca gaagaagctg 1381 cacctgagcg gagtgggaat cgccctgctg atctgcctgt tcctggacaa gcagtacatc 1441 aacatctttc tgagcaggct gcccatcttc tccagctaca atgcccagag cgaggaacgg 1501 cggatcatca tcagatcctt cggcatcaac agcatcaagc tgcccaagga ccggatccac 1561 agcgagaagt ccaacaagag cgtggccatg gatatgctca acgaagtgaa gcggtgcccc 1621 gacgagctgt tcacaacact gtctgccgag aagcagtccc ggttcagaat catcagcgac 1681 gaccacaatg aagtgctgat gaagcggagc agcgacagat tcgtgcctct gctgctgcag 1741 tatatcgatt acggcaagct gttcgaccac atcaggttcc acgtgaacat gggcaagctg 1801 agatacctgc tgaaggccga caagacctgc atcgacggcc agaccagagt cagagtgatc 1861 gagcagcccc tgaacggctt cggcagactg gaagaggccg agacaatgcg gaagcaagag 1921 aacggcacct tcggcaacag cggcatccgg atcagagact tcgagaacat gaagcgggac 1981 gacgccaatc ctgccaacta tccctacatc gtggacacct acacacacta catcctggaa 2041 aacaacaagg tcgagatgtt tatcaacgac aaagaggaca gcgccccact gctgcccgtg 2101 atcgaggatg atagatacgt ggtcaagaca atccccagct gccggatgag caccctggaa 2161 attccagcca tggccttcca catgtttctg ttcggcagca agaaaaccga gaagctgatc 2221 gtggacgtgc acaaccggta caagagactg ttccaggcca tgcagaaaga agaagtgacc 2281 gccgagaata tcgccagctt cggaatcgcc gagagcgacc tgcctcagaa gatcctggat 2341 ctgatcagcg gcaatgccca cggcaaggat gtggacgcct tcatcagact gaccgtggac 2401 gacatgctga ccgacaccga gcggagaatc aagagattca aggacgaccg gaagtccatt 2461 cggagcgccg acaacaagat gggaaagaga ggcttcaagc agatctccac aggcaagctg 2521 gccgacttcc tggccaagga catcgtgctg tttcagccca gcgtgaacga tggcgagaac 2581 aagatcaccg gcctgaacta ccggatcatg cagagcgcca ttgccgtgta cgatagcggc 2641 gacgattacg aggccaagca gcagttcaag ctgatgttcg agaaggcccg gctgatcggc 2701 aagggcacaa cagagcctca tccatttctg tacaaggtgt tcgcccgcag catccccgcc 2761 aatgccgtcg agttctacga gcgctacctg atcgagcgga agttctacct gaccggcctg 2821 tccaacgaga tcaagaaagg caacagagtg gatgtgccct tcatccggcg ggaccagaac 2881 aagtggaaaa cacccgccat gaagaccctg ggcagaatct acagcgagga tctgcccgtg 2941 gaactgccca gacagatgtt cgacaatgag atcaagtccc acctgaagtc cctgccacag 3001 atggaaggca tcgacttcaa caatgccaac gtgacctatc tgatcgccga gtacatgaag 3061 agagtgctgg acgacgactt ccagaccttc taccagtgga accgcaacta ccggtacatg 3121 gacatgctta agggcgagta cgacagaaag ggctccctgc agcactgctt caccagcgtg 3181 gaagagagag aaggcctctg gaaagagcgg gcctccagaa cagagcggta cagaaagcag 3241 gccagcaaca agatccgcag caaccggcag atgagaaacg ccagcagcga agagatcgag 3301 acaatcctgg ataagcggct gagcaacagc cggaacgagt accagaaaag cgagaaagtg 3361 atccggcgct acagagtgca ggatgccctg ctgtttctgc tggccaaaaa gaccctgacc 3421 gaactggccg atttcgacgg cgagaggttc aaactgaaag aaatcatgcc cgacgccgag 3481 aagggaatcc tgagcgagat catgcccatg agcttcacct tcgagaaagg cggcaagaag 3541 tacaccatca ccagcgaggg catgaagctg aagaactacg gcgacttctt tgtgctggct 3601 agcgacaaga ggatcggcaa cctgctggaa ctcgtgggca gcgacatcgt gtccaaagag 3661 gatggatccc ttcaactgcc tccacttgaa agactgacac tgggatccca gctgcattta 3721 ccgcaggttt tagctgacgc tgtctcacgc ctggtcctgg gtaagtttgg tgacctgacc 3781 gacaacttct cctcccctca cgctcgcaga aaagtgctgg ctggagtcgt catgacaaca 3841 ggcacagatg ttaaagatgc caaggtgata agtgtttcta caggaggcaa atgtattaat 3901 ggtgaataca tgagtgatcg tggccttgca ttaaatgact gccatgcaga aataatatct 3961 cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa taacaaagat 4021 gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct gaaggagaat 4081 gtccagtttc atctgtacat cagcacctct ccctgtggag atgccagaat cttctcacca 4141 catgagccaa tcctggaaga accagcagat agacacccaa atcgtaaagc aagaggacag 4201 ctacggacca aaatagagtc tggtcagggg acgattccag tgcgctccaa tgcgagcatc 4261 caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg cagtgacaag 4321 attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt cgtggagccc 4381 atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct ttccagggcc 4441 atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct caacaagcct 4501 ttgctcagtg gcatcagcaa tgcagaagca cggcagccag ggaaggcccc caacttcagt 4561 gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac tgggaaggat 4621 gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg gatgcgtgtg 4681 cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa cgtgtaccat 4741 gagtccaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt cacagccttc 4801 atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca gttctcactc 4861 acgtaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt 4921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga 4981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag 5041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga 5101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac 5161 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg 5221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 5281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 5341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 5401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 5461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 5581 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta 5641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 5701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 5761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta 5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 5881 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga 5941 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa 6001 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 6061 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 6121 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 6181 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg 6241 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 6301 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 6361 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 6421 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 6481 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 6541 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 6601 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 6661 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 6721 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 6781 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa 6841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 6901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 6961 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 7021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 7081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 7141 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 7201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 7261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 7321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 7381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 7441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 7501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 7561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 7621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 7681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 7741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 7801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 7861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 7921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 7981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 8041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 8101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 8161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 8221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 8281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 8341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 8401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 8461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 8521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 8581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 8641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 8701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 8761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 8821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 8881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 8941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 9001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 9061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 9121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 9181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 9241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 9301 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct 9361 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt 9421 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga 9481 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcg //
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