pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

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联系方式:I47-825O-882O

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载体基本信息

质粒名称: pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 
出品公司: 爱迪基因
目录编号: 103871
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: pcDNA3.1
载体出品公司: --
载体抗性: 氨苄青霉素
空载体大小: 5428 bp
完整质粒大小: 9525 bp
载体修饰: 人细胞色素c氧化酶亚基VIII(human cytochrome C oxidase subunit VIII)
载体类型: 哺乳细胞表达
筛选标记: 新霉素G418
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: PspCas13b
物种来源: --
插入大小: 2949 bp
基因突变: --
GenBank编号 --
启动子 --
标签/融合蛋白: 人细胞色素c氧化酶亚基VIII(N端)
克隆方法: 限制性内切酶
5' 克隆位点: AgeI(未破坏)
3' 克隆位点: NotI (未破坏)
5' 测序引物 CMV-F
3' 测序引物 SV40pA-R

文献引用方法
材料和方法部分: pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090 was a gift from Feng Zhang (Addgene plasmid # 103871 ; http://n2t.net/addgene:103871 ; RRID:Addgene_103871)
参考文献部分: RNA editing with CRISPR-Cas13. Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F. Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180. 10.1126/science.aaq0180 PubMed 29070703

载体图谱质粒图谱和多克隆位点信息

pC0055-CMV-dPspCas13b-GS质粒图谱

载体简介

载体序列

LOCUS       pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090                9528 bp ds-DNA     circular SYN 29-AUG-2018
DEFINITION  dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to 
            selectively edit adenosine to inosine in RNA when used in conjuction
            with a guide RNA. Cas13b is truncated to allow for expression by 
            AAV.
ACCESSION   .
VERSION     .
KEYWORDS    pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung 
            J, Zhang F
  TITLE     RNA editing with CRISPR-Cas13.
  JOURNAL   Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.
  PUBMED    29070703
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 29, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        407..610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     561..581
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     655..674
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        655..673
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3676..3702
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(4911..4928)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4917..5141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5187..5615
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5274..5293)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5484..5505
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5624..5644)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5629..5958
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5809..5944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5871..5890
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6025..6819
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6079..6098)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6689..6708
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6993..7114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7030..7049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7084..7103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7163..7179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7163..7179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7176..7198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7187..7203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7211..7241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7256..7277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7394..7411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7565..8153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7645..7664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8324..9184)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8947..8966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9185..9289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9364..9383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      661 gactcactat agggagaccc aagctggcta gcgtttaaac ttaagcttgc caccatgaac
      721 atccccgctc tggtggaaaa ccagaagaag tactttggca cctacagcgt gatggccatg
      781 ctgaacgctc agaccgtgct ggaccacatc cagaaggtgg ccgatattga gggcgagcag
      841 aacgagaaca acgagaatct gtggtttcac cccgtgatga gccacctgta caacgccaag
      901 aacggctacg acaagcagcc cgagaaaacc atgttcatca tcgagcggct gcagagctac
      961 ttcccattcc tgaagatcat ggccgagaac cagagagagt acagcaacgg caagtacaag
     1021 cagaaccgcg tggaagtgaa cagcaacgac atcttcgagg tgctgaagcg cgccttcggc
     1081 gtgctgaaga tgtacaggga cctgaccaac gcatacaaga cctacgagga aaagctgaac
     1141 gacggctgcg agttcctgac cagcacagag caacctctga gcggcatgat caacaactac
     1201 tacacagtgg ccctgcggaa catgaacgag agatacggct acaagacaga ggacctggcc
     1261 ttcatccagg acaagcggtt caagttcgtg aaggacgcct acggcaagaa aaagtcccaa
     1321 gtgaataccg gattcttcct gagcctgcag gactacaacg gcgacacaca gaagaagctg
     1381 cacctgagcg gagtgggaat cgccctgctg atctgcctgt tcctggacaa gcagtacatc
     1441 aacatctttc tgagcaggct gcccatcttc tccagctaca atgcccagag cgaggaacgg
     1501 cggatcatca tcagatcctt cggcatcaac agcatcaagc tgcccaagga ccggatccac
     1561 agcgagaagt ccaacaagag cgtggccatg gatatgctca acgaagtgaa gcggtgcccc
     1621 gacgagctgt tcacaacact gtctgccgag aagcagtccc ggttcagaat catcagcgac
     1681 gaccacaatg aagtgctgat gaagcggagc agcgacagat tcgtgcctct gctgctgcag
     1741 tatatcgatt acggcaagct gttcgaccac atcaggttcc acgtgaacat gggcaagctg
     1801 agatacctgc tgaaggccga caagacctgc atcgacggcc agaccagagt cagagtgatc
     1861 gagcagcccc tgaacggctt cggcagactg gaagaggccg agacaatgcg gaagcaagag
     1921 aacggcacct tcggcaacag cggcatccgg atcagagact tcgagaacat gaagcgggac
     1981 gacgccaatc ctgccaacta tccctacatc gtggacacct acacacacta catcctggaa
     2041 aacaacaagg tcgagatgtt tatcaacgac aaagaggaca gcgccccact gctgcccgtg
     2101 atcgaggatg atagatacgt ggtcaagaca atccccagct gccggatgag caccctggaa
     2161 attccagcca tggccttcca catgtttctg ttcggcagca agaaaaccga gaagctgatc
     2221 gtggacgtgc acaaccggta caagagactg ttccaggcca tgcagaaaga agaagtgacc
     2281 gccgagaata tcgccagctt cggaatcgcc gagagcgacc tgcctcagaa gatcctggat
     2341 ctgatcagcg gcaatgccca cggcaaggat gtggacgcct tcatcagact gaccgtggac
     2401 gacatgctga ccgacaccga gcggagaatc aagagattca aggacgaccg gaagtccatt
     2461 cggagcgccg acaacaagat gggaaagaga ggcttcaagc agatctccac aggcaagctg
     2521 gccgacttcc tggccaagga catcgtgctg tttcagccca gcgtgaacga tggcgagaac
     2581 aagatcaccg gcctgaacta ccggatcatg cagagcgcca ttgccgtgta cgatagcggc
     2641 gacgattacg aggccaagca gcagttcaag ctgatgttcg agaaggcccg gctgatcggc
     2701 aagggcacaa cagagcctca tccatttctg tacaaggtgt tcgcccgcag catccccgcc
     2761 aatgccgtcg agttctacga gcgctacctg atcgagcgga agttctacct gaccggcctg
     2821 tccaacgaga tcaagaaagg caacagagtg gatgtgccct tcatccggcg ggaccagaac
     2881 aagtggaaaa cacccgccat gaagaccctg ggcagaatct acagcgagga tctgcccgtg
     2941 gaactgccca gacagatgtt cgacaatgag atcaagtccc acctgaagtc cctgccacag
     3001 atggaaggca tcgacttcaa caatgccaac gtgacctatc tgatcgccga gtacatgaag
     3061 agagtgctgg acgacgactt ccagaccttc taccagtgga accgcaacta ccggtacatg
     3121 gacatgctta agggcgagta cgacagaaag ggctccctgc agcactgctt caccagcgtg
     3181 gaagagagag aaggcctctg gaaagagcgg gcctccagaa cagagcggta cagaaagcag
     3241 gccagcaaca agatccgcag caaccggcag atgagaaacg ccagcagcga agagatcgag
     3301 acaatcctgg ataagcggct gagcaacagc cggaacgagt accagaaaag cgagaaagtg
     3361 atccggcgct acagagtgca ggatgccctg ctgtttctgc tggccaaaaa gaccctgacc
     3421 gaactggccg atttcgacgg cgagaggttc aaactgaaag aaatcatgcc cgacgccgag
     3481 aagggaatcc tgagcgagat catgcccatg agcttcacct tcgagaaagg cggcaagaag
     3541 tacaccatca ccagcgaggg catgaagctg aagaactacg gcgacttctt tgtgctggct
     3601 agcgacaaga ggatcggcaa cctgctggaa ctcgtgggca gcgacatcgt gtccaaagag
     3661 gatggatccc ttcaactgcc tccacttgaa agactgacac tgggatccca gctgcattta
     3721 ccgcaggttt tagctgacgc tgtctcacgc ctggtcctgg gtaagtttgg tgacctgacc
     3781 gacaacttct cctcccctca cgctcgcaga aaagtgctgg ctggagtcgt catgacaaca
     3841 ggcacagatg ttaaagatgc caaggtgata agtgtttcta caggaggcaa atgtattaat
     3901 ggtgaataca tgagtgatcg tggccttgca ttaaatgact gccatgcaga aataatatct
     3961 cggagatcct tgctcagatt tctttataca caacttgagc tttacttaaa taacaaagat
     4021 gatcaaaaaa gatccatctt tcagaaatca gagcgagggg ggtttaggct gaaggagaat
     4081 gtccagtttc atctgtacat cagcacctct ccctgtggag atgccagaat cttctcacca
     4141 catgagccaa tcctggaaga accagcagat agacacccaa atcgtaaagc aagaggacag
     4201 ctacggacca aaatagagtc tggtcagggg acgattccag tgcgctccaa tgcgagcatc
     4261 caaacgtggg acggggtgct gcaaggggag cggctgctca ccatgtcctg cagtgacaag
     4321 attgcacgct ggaacgtggt gggcatccag ggatcactgc tcagcatttt cgtggagccc
     4381 atttacttct cgagcatcat cctgggcagc ctttaccacg gggaccacct ttccagggcc
     4441 atgtaccagc ggatctccaa catagaggac ctgccacctc tctacaccct caacaagcct
     4501 ttgctcagtg gcatcagcaa tgcagaagca cggcagccag ggaaggcccc caacttcagt
     4561 gtcaactgga cggtaggcga ctccgctatt gaggtcatca acgccacgac tgggaaggat
     4621 gagctgggcc gcgcgtcccg cctgtgtaag cacgcgttgt actgtcgctg gatgcgtgtg
     4681 cacggcaagg ttccctccca cttactacgc tccaagatta ccaagcccaa cgtgtaccat
     4741 gagtccaagc tggcggcaaa ggagtaccag gccgccaagg cgcgtctgtt cacagccttc
     4801 atcaaggcgg ggctgggggc ctgggtggag aagcccaccg agcaggacca gttctcactc
     4861 acgtaagcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
     4921 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     4981 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5041 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5101 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5161 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     5221 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     5281 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     5341 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     5401 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     5461 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     5521 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     5581 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     5641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5881 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5941 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     6001 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     6061 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     6121 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     6181 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     6241 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     6301 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     6361 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     6421 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     6481 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     6541 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     6601 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     6661 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     6721 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     6781 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     6841 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
     6901 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     6961 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     7021 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7081 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     7141 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7201 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7261 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7321 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7381 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7441 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7501 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7561 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7621 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7681 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7741 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7801 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7861 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7921 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7981 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8041 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8101 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8161 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8221 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8281 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8341 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8401 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8461 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8521 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8581 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8641 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8701 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8761 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8821 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8881 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8941 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9001 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9061 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9301 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     9361 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     9421 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     9481 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcg
//

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