| 质粒名称: | pOpen-ECOpolA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165532 |
| 存储实验室: | Drew Endy, Jennifer Molloy, FreeGenes Project |
|
载体骨架基本信息 |
|
| 载体名称: | pBuild |
| 完整质粒大小: | 4910 bp |
| 载体类型: | Synthetic Biology |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | E. coli DNA Polymerase I |
| 物种来源: | 其他 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AATG (unknown if destroyed) |
| 3’克隆位点: | GCTT (unknown if destroyed) |
| 5’测序引物 | M13 forward |
| 3’测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pOpen-ECOpolA was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165532 ; http://n2t.net/addgene:165532 ; RRID:Addgene_165532) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pOpen-ECOpolA 4910 bp ds-DNA circular SYN 05-AUG-2021
DEFINITION DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease
activity. .
ACCESSION .
VERSION .
KEYWORDS pOpen-ECOpolA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4910)
TITLE Open Enzyme Collection
REFERENCE 2 (bases 1 to 4910)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 5, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4910
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2933..2951)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2947..2994
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2999..3015)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2999..3015)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin complement(3082..3670)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3162..3181)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3861..4721)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4484..4503
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4722..4826)
/gene="bla"
/label=AmpR promoter
primer_bind 4842..4858
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 4859..4890
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 aaaggggccg tcaatatcag ggtctctaat ggtgcagatc ccgcaaaacc cgctgatctt
61 ggtggatggc tcatcctacc tgtaccgtgc ttatcacgcg ttcccgccac tgacaaactc
121 tgcgggtgaa ccgaccggcg ctatgtacgg tgtgctgaac atgctgcgct ccctgatcat
181 gcagtacaaa ccgacacatg cggctgtggt tttcgacgcc aagggtaaaa cctttcgtga
241 cgaactgttt gaacattaca aatcccaccg tccgccgatg ccggatgatc tgcgcgctca
301 gatcgaaccg ctgcacgcca tggttaaagc tatgggcctg ccgctgctgg ctgtttccgg
361 tgtagaggcg gacgatgtga tcggcaccct ggcccgcgaa gcggagaagg ctggtcgtcc
421 ggtactgatc tccaccggtg ataaagatat ggcgcagctg gtcaccccga acatcacctt
481 aatcaacacc atgacaaaca cgatcttggg gccggaagaa gttgtgaata aatatggcgt
541 acctccggaa ctgatcatcg actttctggc ccttatgggt gactcctccg acaacattcc
601 gggtgtacct ggcgtggggg aaaagactgc acaggctttg ctgcaaggcc tgggtggcct
661 ggacacgttg tatgcggaac cggaaaaaat cgcgggcctg agcttccgtg gcgcgaaaac
721 tatggcggct aaacttgaac agaacaaaga agtcgcttat ctgtcttacc agctggctac
781 catcaaaacc gacgtggaac tggagcttac ttgcgaacag cttgaagtgc agcagccggc
841 agcggaagaa ctgctgggac tgttcaaaaa atacgaattt aaacgctgga ccgcggacgt
901 ggaggcgggt aaatggctgc aagctaaagg tgcaaagccg gccgcgaaac cgcaggaaac
961 gtctgtagct gatgaggcgc cggaagtgac cgccaccgtt atctcctacg ataattacgt
1021 caccatcctg gacgaggaaa cgctgaaagc atggattgct aaactggaaa aagcgccggt
1081 ttttgcgttc gataccgaaa ctgattctct ggacaacatc agcgcaaacc tggtcggctt
1141 atcttttgct attgaaccgg gtgtggctgc gtatattccg gtggcccacg attatctgga
1201 tgccccggat cagatttccc gtgaacgtgc tctggaactg ctgaaaccgc tgctggagga
1261 tgagaaggca ctgaaagtgg gccagaacct gaaatacgat cgtggtatcc tggcgaacta
1321 cggcatcgag ctgcgtggca ttgcctttga taccatgctg gaatcctaca ttctgaactc
1381 cgtggcaggt cgtcacgata tggattctct ggcggaacgt tggctgaaac acaaaaccat
1441 cacctttgag gaaatcgccg gtaaaggcaa aaaccagctc accttcaacc agatcgcgct
1501 ggaagaggcg ggtcgctatg ccgcagagga cgccgacgtt actctgcaac tgcacctgaa
1561 aatgtggccg gaccttcaga aacacaaagg tccgctgaac gtgttcgaaa atatcgaaat
1621 gccgctggta ccggtgttat cccgtatcga acgtaatggt gttaaaatcg acccgaaagt
1681 tctgcataac cactctgaag aacttactct gcgtctggcg gagctggaaa agaaggctca
1741 cgaaatcgca ggtgaagaat ttaacctttc tagcaccaaa cagctgcaaa ctattctgtt
1801 cgaaaaacag ggtatcaagc cgctgaagaa aaccccgggt ggtgcgccgt ccacctctga
1861 agaagttctg gaagaactgg cactggatta tccactgccg aaagttattc tggaataccg
1921 tggcctggca aagctgaaat cgacttacac ggacaaactg ccgctgatga tcaacccgaa
1981 aaccggccgt gttcacacct cttatcacca ggctgttact gcgactggcc gtctgagctc
2041 taccgacccg aacctgcaaa acatcccggt gcgtaatgaa gaaggtcgtc gtatccgtca
2101 ggccttcatc gcaccggagg attacgtaat cgtttctgcg gactactccc agattgagct
2161 gcgcatcatg gctcatctgt cccgtgataa gggtctgctg actgcgtttg ctgaaggtaa
2221 agacattcac cgtgctaccg cggcggaagt atttggcctg ccgctggaaa cggttactag
2281 cgaacagcgt cgcagcgcta aagcgatcaa cttcggtctg atctacggca tgtccgcatt
2341 tggcctggca cgtcaactga acattccgcg taaagaggcg cagaagtaca tggatctgta
2401 cttcgaacgt tacccgggcg tgcttgaata catggagcgt acccgtgccc aagccaaaga
2461 gcagggttac gttgaaaccc tggatggccg tcgtttatac ctgccggata ttaaatcctc
2521 caacggcgcc cgtcgtgcag cggcagagcg tgccgccatc aacgcaccga tgcagggcac
2581 cgcagcggac attattaaac gtgcaatgat tgcggtagac gcttggctgc aagcggaaca
2641 gccgcgtgtt cgtatgatca tgcaagtgca tgacgaactg gttttcgaag tacataaaga
2701 cgatgtcgat gctgtagcca aacagattca tcaactgatg gaaaactgca cccgcctgga
2761 cgtgcctctg ctggttgaag tgggctccgg cgaaaactgg gaccaagcac attgaagagc
2821 tttgagacca cataagcgat cccaaggtcc ccaggttaag gcatgcatat tagggataac
2881 agggtaatcc agcacaaacg atgttgaatc gctgaggtgt caatcgtcgg agccgctgag
2941 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgcatggt
3001 catagctgtt tcctgagagc ttggcaggtg atgacacaca ttaacaaatt tcgtgaggag
3061 tctccagaag aatgccatta atttccatag gctccgcccc cctgacgagc atcacaaaaa
3121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
3181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
3241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
3301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
3361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
3421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
3481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
3541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacac gcagaaaaaa
3661 aggatctcaa gaaggcctac tattagcaac aacgatcctt tgatcttttc tacggggtct
3721 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
3781 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
3841 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
3901 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
3961 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
4021 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
4081 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
4141 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
4201 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
4261 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
4321 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
4381 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
4441 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
4501 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
4561 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
4621 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
4681 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
4741 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
4801 aaaaataaac aaataggggt tccgcgcacc tgcaccagtc agtaaaacga cggccagtag
4861 tcaaaagcct ccgaccggag gcttttgact tggttcaggt ggagtgggag
//
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