| 质粒名称: | pX037 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 167156 |
| 存储实验室: | Karen Sarkisyan |
|
载体骨架基本信息 |
|
| 载体名称: | Level2-like |
| 载体出品公司: | -- |
| 空载体大小: | 4839 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | caffeic acid cycle genes |
| 物种来源: | Escherichia coli, Neonothopanus nambi |
| 插入大小: | 15128 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | pNOS, p35s |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BbsI（克隆期间销毁） |
| 3’克隆位点: | BbsI（克隆期间销毁） |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pX037 was a gift from Karen Sarkisyan (Addgene plasmid # 167156 ; http://n2t.net/addgene:167156 ; RRID:Addgene_167156) |
| 参考文献部分: | Plants with genetically encoded autoluminescence. Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, Sarkisyan KS. Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi: 10.1038/s41587-020-0500-9. 10.1038/s41587-020-0500-9 PubMed 32341562 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX037 19895 bp ds-DNA circular SYN 17-JUN-2021
DEFINITION MoClo-compatible Level2 vector for autonomous bioluminescence in
plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz
and nnCPH. Does not contain NpgA..
ACCESSION .
VERSION .
KEYWORDS pX037
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 19895)
AUTHORS Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
Sarkisyan KS
TITLE Plants with genetically encoded autoluminescence.
JOURNAL Nat Biotechnol. 2020 Apr 27. pii: 10.1038/s41587-020-0500-9. doi:
10.1038/s41587-020-0500-9.
PUBMED 32341562
REFERENCE 2 (bases 1 to 19895)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 17, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..19895
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 314..333
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 749..1897
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
misc_feature 2043..2067
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
promoter 2230..2409
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 2440..2494
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 2496..3290
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MVEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2550..2569)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3160..3179
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
terminator 3317..4024
/label=OCS terminator
/note="octopine synthase terminator"
promoter 4124..4469
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 9663..10370
/label=OCS terminator
/note="octopine synthase terminator"
promoter 10475..10820
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 12186..12893
/label=OCS terminator
/note="octopine synthase terminator"
promoter 12998..13343
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS complement(13904..13915)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
CDS 14241..14258
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 14286..14993
/label=OCS terminator
/note="octopine synthase terminator"
promoter 15098..15443
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
terminator 16461..17168
/label=OCS terminator
/note="octopine synthase terminator"
protein_bind 17242..17263
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 17278..17308
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 17316..17332
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 17321..17343
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 17340..17356
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 17340..17356
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 17352..17675
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
misc_feature 17369..17425
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(17426..17443)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(17426..17442)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(17435..17457)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(17651..17670)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
misc_feature 17807..17831
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
rep_origin 17919..18629
/label=oriV
/note="incP origin of replication"
promoter 18650..18754
/gene="bla"
/label=AmpR promoter
CDS 18755..19549
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 19720..413
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
61 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
121 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
181 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
241 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
301 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
361 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
421 gcaacgcggc ctttttacgg ttcctggcag atcctagatg tggcgcaacg atgccggcga
481 caagcaggag cgcaccgact tcttccgcat caagtgtttt ggctctcagg ccgaggccca
541 cggcaagtat ttgggcaagg ggtcgctggt attcgtgcag ggcaagattc ggaataccaa
601 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
661 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
721 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
781 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
841 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
901 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
961 cgccgtggag cgttcgcgtc gtcttgaaca ggaggcggca ggtttggcga agtcgatgac
1021 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
1081 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
1141 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
1201 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
1261 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
1321 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
1381 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
1441 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
1501 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
1561 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
1621 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
1681 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
1741 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
1801 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
1861 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
1921 tgggggttca gcagcccctg ctcggatctg ttggaccgga cagtagtcat ggttgatggg
1981 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
2041 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
2101 cggacgtttt taatgtactg gggttgaaca ctctgtgccg aattcggatc cggagcggag
2161 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
2221 gaactgacag aaccgcaacg ttgaaggagc cactgagccg cgggtttctg gagtttaatg
2281 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat
2341 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt
2401 atccaattag agtctcatat tcactctcct atttttacaa caattaccaa caacaacaaa
2461 caacaaacaa cattacaatt acatttacaa ttaccatggt tgaacaagat ggattgcacg
2521 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
2581 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
2641 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
2701 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
2761 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
2821 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
2881 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
2941 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
3001 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgactcatg
3061 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
3121 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
3181 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
3241 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
3301 ggggttcgct agagtcctgc tttaatgaga tatgcgagac gcctatgatc gcatgatatt
3361 tgctttcaat tctgttgtgc acgttgtaaa aaacctgagc atgtgtagct cagatcctta
3421 ccgccggttt cggttcattc taatgaatat atcacccgtt actatcgtat ttttatgaat
3481 aatattctcc gttcaattta ctgattgtac cctactactt atatgtacaa tattaaaatg
3541 aaaacaatat attgtgctga ataggtttat agcgacatct atgatagagc gccacaataa
3601 caaacaattg cgttttatta ttacaaatcc aattttaaaa aaagcggcag aaccggtcaa
3661 acctaaaaga ctgattacat aaatcttatt caaatttcaa aagtgcccca ggggctagta
3721 tctacgacac accgagcggc gaactaataa cgctcactga agggaactcc ggttccccgc
3781 cggcgcgcat gggtgagatt ccttgaagtt gagtattggc cgtccgctct accgaaagtt
3841 acgggcacca ttcaacccgg tccagcacgg cggccgggta accgacttgc tgccccgaga
3901 attatgcagc atttttttgg tgtatgtggg ccccaaatga agtgcaggtc aaaccttgac
3961 agtgacgaca aatcgttggg cgggtccagg gcgaattttg cgacaacatg tcgaggctca
4021 gccgctgcaa gaattcaagc ttggaggtca acatggtgga gcacgacact ctggtctact
4081 ccaaaaatgt caaagataca gtctcagaag atcaaagggc tattgagact tttcaacaaa
4141 ggataatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
4201 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
4261 tcattcaaga tctctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
4321 tcgtggaaaa agaagaggtt ccaaccacgt ctacaaagca agtggattga tgtgacatct
4381 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
4441 aaggaagttc atttcatttg gagaggacac gctcgagtat aagagctcat ttttacaaca
4501 attaccaaca acaacaaaca acaaacaaca ttacaattac atttacaatt atcgatacaa
4561 tgaactcaag taagaatccg ccctccacat tgttggatgt atttctcgac acggctagaa
4621 atcttgatac cgctagtagg aatgtcctgg aatgcgggga acatcgttgg tcatatcgtg
4681 aattggatac ggtaagctct gccctggccc agcaccttcg atataccgtc ggcttgagtc
4741 ctacagtagc cgtgatcagt gagaaccacc cttacatatt ggccttgatg ttggccgttt
4801 ggaaattggg gggtaccttc gctccaatcg acgtacatag tcccgccgag ctcgtggctg
4861 gcatgttgaa tatagttagc cccagctgtc tggttatacc ttctagtgac gttaccaacc
4921 agacattggc ctgcgatctc aatataccag tagtagcttt ccaccctcat caatcaacga
4981 taccggaact gaacaaaaag tacttgactg acagtcaaat atcacctgac ttgcctttcc
5041 ccgaccccaa caggccggct ctttatctgt ttaccagcag cgctacttct cgttctaatc
5101 tgaaatgcgt ccccttgacg catacattta tccttagaaa ctctctttca aaaagggcct
5161 ggtgcaaacg tatgaggccc gaaaccgatt ttgatggtat tcgtgtactc ggttgggctc
5221 cttggtccca cgtgcttgca catatgcagg acattggacc attgacgctt ttgaacgcag
5281 ggtgctacgt ttttgctacg actccttcaa catatccgac agagctgaag gacgatcgag
5341 atgtgatctc ctgtgccgcc aacgcagtca tgtataaggg agttaagtca tttgcctgtc
5401 taccttttgt gttgggcgga cttaaagcac tctgcgaaag cgagccatct gtaaaagctc
5461 agttgcaagt ggaagagcga gcacaattgc tcaaatccct gcagcatatg aagatcctgg
5521 agtgcggtgg tgccatgttg gaagtctccg ttgcatcctg ggcaatagag aacagaatcc
5581 ccataagcat tgggataggc atgactgaaa ctggtggagc cctcttcgct ggaccagttc
5641 aggcaataca aacaggattt tcatcagagg acaagttcat agaggacgcc acttatcttt
5701 tggtgaagga tgactatgag tcccacgccg aggaagatat taacgaaggt gaactcgtcg
5761 tcaagtccag gatgctgcct cgtgggtatc tggggtataa tgatccatca ttcagcgttg
5821 atgatgcagg ctgggtgacg ttcaaaacgg gagataggta cagtgttaca cccgacggca
5881 aatttagctg gctggggaga aataccgact tcattcagat gacatctggt gaaaccttgg
5941 accctcgtcc gatagaaagt ctcctctgtg agtcctcact tatttctagg gcctgcgtaa
6001 ttggcgataa gtttcttaac gggccagcaa ctgctgtttg tgccataatc gaactggaac
6061 caaccaccgt tgagaagggt caagctcatt ccagagacat agctaggata ttcgccccca
6121 tcaacaggga tttgcccccc cctttgcgta tcgcatggtc acatgtcctg gttttgcagc
6181 ctagtgagaa gatacctatg acaaagaagg ggacgatttt tcgaaaaaag atcgaacaag
6241 tctttgggtc cgcattgggt ggatcatccg gcgacaatag ccaagctact actgacgcta
6301 gcgtggtaag acgtgacgaa ctcagtaaca cagtgaagca cattatatcc cgagtgttgg
6361 gggtgtctga tgacgaactc ttgtggaccc tctctttcgc cgagttgggt atgacttctg
6421 ctttggccac caggattgca aatgagttga acgaagtcct tgtgggagtg aacttgccta
6481 taaacgcctg ctatattcat gtggatcttc cgagcctcag taatgcagtc tatgccaaac
6541 tcgcacactt gaagttgcca gaccgtactc cggaaccacg aaaggcacca gttgaaaatc
6601 ccggaggtaa agagatcgtc attgtaggcc aggccttccg tttgcccggg agtatcaacg
6661 acgtcgcctc tcttagggat gcctttttgg ctaggcaggc cagctcaatt attaccgaga
6721 tacctccgga caggtgggac cacgcatcct tctacccaaa agatattcgt ttcaataagg
6781 ctgggctggt ggacatcgcc aactacgatc attccttctt tgggctgacc gccacggaag
6841 cactctatct cagccctacg atgcgactcg ccttggaggt gtcatttgaa gcattggaga
6901 atgccaacat tccagtttct caactgaagg gctcccagac agcagtgtat gttgcaacaa
6961 cagacgatgg ttttgaaacg ttgctcaacg ccgaagcagg ttacgatgcc tacaccagat
7021 tttacgggac tgggagggct gcctctacgg catcaggccg tataagttat ctcctcgatg
7081 tacatggacc atccatcact gtggacacag cctgcagtgg aggagcagtg tgcatagatc
7141 aagccattga ttatctccag tcctcaagtg ctgctgatac cgctattatt tgtgctagta
7201 atacccattg ctggccgggg tcatttatgt ttctcagcgc tcaaggtatg gtttcatctg
7261 ggggtagatg tgctacattc acgactgatg cagacggata tgttccatcc gaaggagctg
7321 tagctttcat acttaagaca agagaggctg ctatgagaga taaagatacc atattggcaa
7381 caataaaggc tacgcaaatt tcccacaacg gacgatccca aggactcgtg gctcctaacg
7441 ttaatagtca agccgatctg catcgtagcc tccttcagaa agccgggctt tcccctgccg
7501 atatacattt catagaagca cacggcactg ggacaagtct cggagacttg tctgaaatcc
7561 aagccattaa cgacgcttat acctctagtc aacccagaac agccggacca ttgattgtgt
7621 ccgcctccaa aactgtgatc gggcacactg aacctgctgg accattggtc ggtatgctct
7681 ctgtactcaa ttcctttaag gaaggggctg tcccggggct tgcccacctt accgccgaca
7741 acttgaatcc ggctttggat tgctctagtg tccctttgtt gattccgtat caaccggtac
7801 atttggctgc acccaagcca catagggccg ctgttatgag ttatgggttc tccggtacac
7861 tgggagggat cgtgttggaa gctccggatg aagagcgtct tgaagaagaa cccccaaacg
7921 acaagccaat gttgtttgta gttagtgcta agacccatac cgccctgatc gagtatttgg
7981 gtagatattt ggagtttttg cttcaagcca acccgcaaga tttctgcgac atatgttaca
8041 catcatgcgt cggccgtgaa cattataggt accgtttcgc ttgcgtcgcc aacgacatgg
8101 aggacctgat cggccaactt caaaaaaggc tggggtccaa ggtaccgcca aaaccgagct
8161 ataagcgagg cgcacttgct tttgcattct ccggtcaagg tactcagttt agaggtatgg
8221 caactgaact ggcaaaagcc tactcaggtt ttaggaagat agtcagtgac ttggctaaga
8281 gggcctccga actctccggc catgctattg ataggttctt gttggcttat gatattggcg
8341 ctgaaaatgt ggcaccagac tcagaagcag accaaatatg catattcgtg taccaatgca
8401 gcgtgctcag atggcttcag accatgggca ttcgtcctag tgctgtaata ggtcactcac
8461 tgggagaaat ctctgcttca gttgcagcag gggcactcag tctcgacagc gccctggatc
8521 tcgtgataag cagggctaga ttgttgagat cttctactaa tgcccccgct ggtatggctg
8581 ccatgtctgc tagccaggat gaggtcgtgg aactcatcgg aaaattggac cttgataaag
8641 ctaactcctt gtcagtctca gtcatcaatg gcccccagaa caccgttgtg tccggtagct
8701 cagcagccat tgaatcaata gtagcattgg ccaagggtag gaaaattaaa gcaagcgctc
8761 ttaatattaa tcaggcattt cactccccat atgttgattc tgcagtaccg ggcttgcgag
8821 cctggtcaga aaagcatatc tcatccgcaa ggccgctcca gataccgttg tattcaacct
8881 tgcttggtgc tcaagtcagc gaagggcaaa tgcttaatcc ggatcactgg gtagatcatg
8941 cccgtaagcc tgttcagttt gctcaagccg caacaatcat gaaagagtcc tttactgggg
9001 tgatcatcga catcggtccc caggtggttg cctggtcact gctcttgagc aacggtttga
9061 catccgtcac tgcactcgcc gctaagcgtg ggcgaagcca acaggtcgct ttcctcagcg
9121 ccttggccga tctgtatcaa gactatggtg tagtacctga ctttgtcggg ttgtacgccc
9181 agcaggagga cgctagcagg cttaaaaaga cagatatttt gacctacccg tttcaaaggg
9241 tcagacgata tccgtctttc attccgtcac gtcgtgcacc gacacatgcc cacgttcagg
9301 acgaggagac tctgtcatcc gggagcagta cgcccacatt ggaaaatacc gaccttgatt
9361 ctggtaagga gagtctcatg ggaccgacaa gagggttgtt gcgtgtcgac gacttgaggg
9421 actccatcgt tagttctgtg aaggacgtac tcgaactcaa atccaacgag gacctcgatt
9481 tgtcagaatc attgaacgct cttgggatgg actccatcat gttcgcacag ttgagaaaga
9541 ggataggcga agggcttgga ttgagtgttc ctatggtttt tctgtcagac gctttctcca
9601 taggtgaaat ggttagcaac ctggtggaac aggctgaggc ttccgaggac aactagcttg
9661 tcctgcttta atgagatatg cgagaagcct atgatcgcat gatatttgct ttcaattctg
9721 ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga tccttaccgc cggtttcggt
9781 tcattctaat gaatatatca cccgttacta tcgtattttt atgaataata ttctccgttc
9841 aatttactga ttgtacccta ctacttatat gtacaatatt aaaatgaaaa caatatattg
9901 tgctgaatag gtttatagcg acatctatga tagagcgcca caataacaaa caattgcgtt
9961 ttattattac aaatccaatt ttaaaaaaag cggcagaacc ggtcaaacct aaaagactga
10021 ttacataaat cttattcaaa tttcaaaagt gccccagggg ctagtatcta cgacacaccg
10081 agcggcgaac taataacgct cactgaaggg aactccggtt ccccgccggc gcgcatgggt
10141 gagattcctt gaagttgagt attggccgtc cgctctaccg aaagttacgg gcaccattca
10201 acccggtcca gcacggcggc cgggtaaccg acttgctgcc ccgagaatta tgcagcattt
10261 ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac cttgacagtg acgacaaatc
10321 gttgggcggg tccagggcga attttgcgac aacatgtcga ggctcagcag gaccgctact
10381 agaattcgag ctcggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg
10441 tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt
10501 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
10561 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
10621 atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
10681 aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc tccactgacg
10741 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
10801 catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac aattaccaac
10861 aacaacaaac aacaaacaac attacaatta catttacaat tatcgataca atggcctctt
10921 ttgaaaattc actctctgtg ttgatcgtgg gagccggttt gggtggtcta gcagctgcta
10981 tcgctctccg acgacagggc cacgtggtta agatctacga ctcatctagc tttaaggccg
11041 agctcggcgc aggcttggca gttccaccga atacactgcg tagtctacag caattgggtt
11101 gtaacactga aaaccttaac ggcgttgata atctctgttt tactgccatg ggatatgatg
11161 ggtcagtggg catgatgaac aatatgactg attaccgtga agcatatggc actagttgga
11221 tcatggttca tcgtgtcgat ttgcataacg agttgatgag agttgctttg gacccagggg
11281 gattgggccc tcccgcaacg cttcacctga atcatcgagt cacgttttgt gatgtagatg
11341 cttgtacggt gacatttacc aacggtacaa ctcaaagtgc agatcttatc gttggggctg
11401 acgggatacg ttcaactatc cgtagatttg tcttggagga ggacgtgacc gttcccgcaa
11461 gcggaattgt ggggtttcga tggttggtac aggctgatgc cctcgatcca tatcctgaac
11521 tcgattggat agtcaaaaag ccaccgctcg gagctaggtt gatttctacg ccccagaatc
11581 cacaatctgg agtgggactg gcagatcgta gaactattat tatatacgct tgtcgtgggg
11641 ggacaatggt aaatgtgctg gccgtacacg atgacgagcg agatcagaat actgcagatt
11701 ggagcgttcc agcatcaaag gatgacttgt ttcgagtatt ccatgactac catccgagat
11761 tcagacggct cttggaattg gctcaagata taaatctctg gcagatgagg gttgtgccgg
11821 tgctcaagaa atgggtgaac aaacgagtgt gcctgcttgg agatgccgct catgcctctc
11881 ttccgactct cggccagggt ttcgggatgg gattggagga cgccgtagcc cttggtactc
11941 tcttgccaaa aggtacgact gctagtcaaa tagagacaag actggctgtc tacgaacagc
12001 ttagaaagga ccgagcagag tttgtagccg ctgaaagtta tgaagagcag tatgtccccg
12061 agatgagggg cttgtacttg cgtagcaagg aacttagaga tagagtcatg gggtatgaca
12121 taaaagtgga gtctgagaag gtactggaaa ccttgcttag gagctctaac tcagcttagc
12181 ttgtcctgct ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt
12241 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
12301 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
12361 ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata
12421 ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc
12481 gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac
12541 tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca
12601 ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg
12661 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
12721 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
12781 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
12841 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaccgct
12901 ttacgaattc ccatggggag gtcaacatgg tggagcacga cactctggtc tactccaaaa
12961 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
13021 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
13081 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
13141 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
13201 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
13261 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
13321 gttcatttca tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc
13381 aacaacaaca aacaacaaac aacattacaa ttacatttac aattatcgat acaatgagga
13441 taaatatctc tttgtctagt ctctttgaga gactgagcaa attgtcatcc cgatccatcg
13501 caattacctg cggcgtagtc ttggcaagtg caatagcatt cccaattatc cgaagagact
13561 atcaaacgtt ccttgaagtc ggtccgagct atgccccaca gaacttccga ggctatatca
13621 tcgtttgtgt cttgtcactt tttaggcaag agcagaaagg gttggcaatc tatgacaggc
13681 ttccagaaaa gaggcgttgg cttgccgact tgccgtttcg tgaagggacg aggccatcca
13741 taacctccca cattatacag cgtcagcgta ctcagctggt agatcaagag tttgcaacca
13801 gagaacttat cgataaggtg atcccacgtg tgcaagcaag acacacagac aaaacttttc
13861 tcagtacgtc aaaatttgag tttcatgcaa aggccatctt ccttctcccc tctatcccta
13921 ttaatgatcc tcttaacatt ccctcacacg atacggtaag aagaaccaag cgtgagatcg
13981 ctcacatgca tgattaccat gattgcactc tgcacttggc tcttgctgct caggatggta
14041 aggaagtttt gaagaagggg tggggccagc gtcacccact ggccggacca ggagtcccag
14101 gccctcctac tgagtggacc ttcctttacg caccaaggaa cgaagaggag gccagagttg
14161 tcgagatgat agtcgaagct agtattggct acatgactaa cgatcctgct ggtaagattg
14221 tcgaaaatgc caagtcaggt caccatcacc atcaccatta gtgcgctacg ctacatcagc
14281 ttgtcctgct ttaatgagat atgcgagaag cctatgatcg catgatattt gctttcaatt
14341 ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac cgccggtttc
14401 ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata atattctccg
14461 ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga aaacaatata
14521 ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac aaacaattgc
14581 gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa cctaaaagac
14641 tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat ctacgacaca
14701 ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc ggcgcgcatg
14761 ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta cgggcaccat
14821 tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa ttatgcagca
14881 tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca gtgacgacaa
14941 atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag caggaccgct
15001 cagagaattc gcatgcggag gtcaacatgg tggagcacga cactctggtc tactccaaaa
15061 atgtcaaaga tacagtctca gaagatcaaa gggctattga gacttttcaa caaaggataa
15121 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
15181 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
15241 aagatctctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
15301 aaaaagaaga ggttccaacc acgtctacaa agcaagtgga ttgatgtgac atctccactg
15361 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
15421 gttcatttca tttggagagg acacgctcga gtataagagc tcatttttac aacaattacc
15481 aacaacaaca aacaacaaac aacattacaa ttacatttac aattatcgat acaatggcac
15541 ctatttcatc cacgtggagc aggttgataa ggttcgtggc tgtagaaact tccctggttc
15601 atattggtga gccaattgat gcaacgatgg acgttggcct cgcccgtaga gaaggtaaga
15661 caattcaagc atacgaaata atcggaagcg gcagtgccct cgatctttct gcacaagtct
15721 ctaagaacgt actcacagtt agggaattgt tgatgccgct cagtcgagaa gagattaaga
15781 ccgttcgatg cctcggcttg aactatccgg tccacgccac agaagctaac gtggccgtgc
15841 cgaagtttcc taacctgttc tataagcccg taacatcatt gattggtcca ggcggtctta
15901 ttactattcc ctccgtcgtt caacccccca aggagcacca gtcagattac gaggctgagc
15961 tggttatagt aataggtaag gcagcaaaga acgtatcaga ggatgaggct ttggattatg
16021 tattgggcta taccgctgca aacgatatat ctttccgtaa gcatcaattg gctgtcagtc
16081 aatggagctt cagcaagggc tttgatggaa ctaatccact tggtccctgc ttggtttccg
16141 cctccagtat ccctgaccca caggatattc caatacagtg caaattgaat ggtggagtcg
16201 tccaaaatgg caacaccaga gatcaaatct tcaacgttaa gaaaaccata tcatttcttt
16261 cccaggggac tactttggag cccggttcca ttatccttac cgggacccca gatggggtgg
16321 ggttcgtacg aaacccgccc ctgtatttga aggatggcga tgaggttatg acttggatcg
16381 gtagtggaat cggaactctc gcaaatacag ttcgagaaga acaaacgtgc tttgcatcag
16441 gcggccacga atagcttgtc ctgctttaat gagatatgcg agaagcctat gatcgcatga
16501 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
16561 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
16621 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
16681 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
16741 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
16801 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
16861 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
16921 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
16981 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
17041 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
17101 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
17161 ctcagcagga ccgcttgtgt gagaccgcag ctggcacgac aggtttcccg actggaaagc
17221 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
17281 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
17341 aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg actctagagg
17401 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
17461 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
17521 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
17581 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
17641 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
17701 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
17761 tgacggtctc agggacacga agtgatccgt ttaaactatc agtgtttgac aggatatatt
17821 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
17881 gaaaaggttt atccgttcgt ccatttgtat gtgccagccg cctttgcgac gctcaccggg
17941 ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg
18001 ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac
18061 ttggccctca ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc
18121 gcggcgttga cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc
18181 tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc
18241 ctggggataa gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg
18301 gcgcgatcct tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca
18361 tttgaggggc tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc
18421 ggccaccgct aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt
18481 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
18541 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
18601 cgcgaacggc ctcaccccaa aaatggcagc gctggccaat tcccgagtgc gcggaacccc
18661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
18721 ataaatgctt caataatatt gaaaaaggaa gagtatggct aaaatgagaa tatcaccgga
18781 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
18841 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
18901 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
18961 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
19021 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
19081 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
19141 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
19201 cgatctggcc gatgtggatt gcgaaaactg ggaagaggac actccattta aagatccgcg
19261 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
19321 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
19381 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgctcag
19441 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
19501 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagc tgtcagacca
19561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
19621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
19681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
19741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
19801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
19861 tactgttctt ctagtgtagc cgtagttagg ccacc
//
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