质粒名称: | pNI4 |
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出品公司: | 爱迪基因 |
目录编号: | 30999 |
存储实验室: | Adam Bogdanove, Daniel Voytas |
载体骨架基本信息 |
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载体名称: | pTC14 |
载体抗性: | 四环素 |
空载体大小: | 2989 bp |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | repeat NI4 |
物种来源: | Xanthamonas oryzae |
插入大小: | 104 bp |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | XbaI(未破坏) |
3' 克隆位点: | XhoI (未破坏) |
5' 测序引物 | pTC14_F2 (caagcctgattgggagaaaa) |
文献引用方法 |
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材料和方法部分: | pNI4 was a gift from Adam Bogdanove & Daniel Voytas (Addgene plasmid # 30999 ; http://n2t.net/addgene:30999 ; RRID:Addgene_30999) |
参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNI4 3099 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pNI4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3099) AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF TITLE Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. JOURNAL Nucleic Acids Res. 2011 Apr 14. ():. PUBMED 21493687 REFERENCE 2 (bases 1 to 3099) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3099 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 196..224 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 272..1462 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" primer_bind complement(624..643) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(1636..1655) /label=T7 /note="T7 promoter, forward primer" promoter complement(1637..1655) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1660..1676) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1660..1676) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" rep_origin 1948..2536 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2437..2456 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2866..2893 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2985..3071 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca 241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg 601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa 781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg 841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga 1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1741 tgcctcctct agaggtctcg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc 1801 gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt ggagaccctc 1861 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga 1921 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1981 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2041 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 2101 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2161 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2221 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2281 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2341 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2401 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2461 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2521 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2581 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 2641 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2701 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 2761 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 2821 aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc 2881 aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac 2941 cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt cctactcagg 3001 agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga ctgagccttt 3061 cgttttattt gatgcctggc agttccctac tctcgcgtt //