| 质粒名称: | pCMV-ABEmaxAW |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 125647 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ABEmax(TadA E59A, TadA* V106W),ABEmaxAW |
| 物种来源: | H. sapiens (human |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-ABEmaxAW was a gift from David Liu (Addgene plasmid # 125647 ; http://n2t.net/addgene:125647 ; RRID:Addgene_125647)) |
| 参考文献部分: | Analysis and minimization of cellular RNA editing by DNA adenine base editors. Rees HA, Wilson C, Doman JL, Liu DR. Sci Adv. 2019 May 8;5(5):eaax5717. doi: 10.1126/sciadv.aax5717. eCollection 2019 May. 10.1126/sciadv.aax5717 PubMed 31086823 |
买家导航
pCMVABEmaxAW载体基本信息
pCMVABEmaxAW载体图谱质粒图谱和多克隆位点信息
pCMVABEmaxAW载体简介
pCMVABEmaxAW载体序列
LOCUS pCMV-ABEmaxAW 8811 bp ds-DNA circular SYN 13-MAY-2019
DEFINITION Expresses ABEmaxAW in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-ABEmaxAW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8811)
TITLE : Analysis and minimization of cellular RNA editing by DNA adenine
base editors
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8811)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8811
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 74..453
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 454..657
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 608..628
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 699..718
/label=T7
/note="T7 promoter, forward primer"
promoter 699..717
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 767..787
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1976..6076
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYYLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6119..6139
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6148..6165
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6188..6205)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6194..6418
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6489..6505)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6489..6505)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6502..6524)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6513..6529
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6537..6567)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6582..6603
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6720..6737)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6891..7479)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6971..6990)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7650..8510)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8273..8292
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8511..8615)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8694..8713)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
661 ttagtgaacc gtcagatccg ctagagatcc gcggccgcta atacgactca ctatagggag
721 agccgccacc atgaaacgga cagccgacgg aagcgagttc gagtcaccaa agaagaagcg
781 gaaagtctct gaagtcgagt ttagccacga gtattggatg aggcacgcac tgaccctggc
841 aaagcgagca tgggatgaaa gagaagtccc cgtgggcgcc gtgctggtgc acaacaatag
901 agtgatcgga gagggatgga acaggccaat cggccgccac gaccctaccg cacacgcagc
961 gatcatggca ctgaggcagg gaggcctggt catgcagaat taccgcctga tcgatgccac
1021 cctgtatgtg acactggagc catgcgtgat gtgcgcagga gcaatgatcc acagcaggat
1081 cggaagagtg gtgttcggag cacgggacgc caagaccggc gcagcaggct ccctgatgga
1141 tgtgctgcac caccccggca tgaaccaccg ggtggagatc acagagggaa tcctggcaga
1201 cgagtgcgcc gccctgctga gcgatttctt tagaatgcgg agacaggaga tcaaggccca
1261 gaagaaggca cagagctcca ccgactctgg aggatctagc ggaggatcct ctggaagcga
1321 gacaccaggc acaagcgagt ccgccacacc agagagctcc ggcggctcct ccggaggatc
1381 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
1441 ggcacgcgat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
1501 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
1561 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
1621 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
1681 cgtggtgttt ggctggagga acgcaaaaac cggcgccgca ggctccctga tggacgtgct
1741 gcactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
1801 tgccgccctg ctgtgctatt tctttcggat gcctagacag gtgttcaatg ctcagaagaa
1861 ggcccagagc tccaccgact ccggaggatc tagcggaggc tcctctggct ctgagacacc
1921 tggcacaagc gagagcgcaa cacctgaaag cagcgggggc agcagcgggg ggtcagacaa
1981 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
2041 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
2101 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
2161 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
2221 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
2281 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
2341 ggacgaggtg gcctaccacg agaagtaccc caccatctac tacctgagaa agaaactggt
2401 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
2461 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
2521 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
2581 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
2641 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
2701 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
2761 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
2821 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
2881 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
2941 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
3001 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
3061 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
3121 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
3181 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
3241 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
3301 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
3361 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
3421 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
3481 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
3541 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
3601 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
3661 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
3721 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
3781 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
3841 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
3901 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
3961 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
4021 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
4081 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
4141 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
4201 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
4261 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
4321 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
4381 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
4441 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
4501 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
4561 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
4621 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
4681 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
4741 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
4801 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
4861 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
4921 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
4981 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
5041 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
5101 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
5161 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
5221 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
5281 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
5341 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
5401 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
5461 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
5521 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
5581 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg
5641 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
5701 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
5761 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
5821 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
5881 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
5941 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
6001 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
6061 tcagctggga ggtgactctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
6121 caagaagaag aggaaagtct aaccggtcat catcaccatc accattgagt ttaaacccgc
6181 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
6241 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
6301 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
6361 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
6421 tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta gagcttggcg
6481 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
6541 atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag ctaactcaca
6601 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
6661 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
6721 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
6781 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
6841 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
7081 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
7321 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
7501 acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
7561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
7621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
7681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
7741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
7801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
7861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
7921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
7981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
8041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
8101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
8161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
8221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
8281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
8341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
8401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
8461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
8521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
8581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
8641 gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc
8701 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
8761 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga c
//
