质粒名称: | pMSCV-Peredox-mCherry-NLS |
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出品公司: | 爱迪基因 |
目录编号: | 32385 |
存储实验室: | Gary Yellen |
载体骨架基本信息 |
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载体名称: | pMSCV |
载体出品公司: | Clontech |
载体抗性: | 氨苄霉素 |
空载体大小: | 6300 bp |
载体类型: | 哺乳细胞表达 |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 未知 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Peredox-mCherry-NLS |
插入大小: | 2800 bp |
标签/融合蛋白: | Nuclear Localization Seq(C端) |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | XhoI(未破坏) |
3' 克隆位点: | EcoRI(克隆期间被破坏) |
5' 测序引物 | CCCTTGAACCTCCTCGTTCGACC |
3' 测序引物 | GAGACGTGCTACTTCCATTTGTC |
文献引用方法 |
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材料和方法部分: | pMSCV-Peredox-mCherry-NLS was a gift from Gary Yellen (Addgene plasmid # 32385) |
参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMSCV-Peredox-mCherry-NLS 9107 bp DNA SYN DEFINITION pMSCV-Peredox-mCherry-NLS ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..9107 /organism="pMSCV-Peredox-mCherry-NLS" /mol_type="other DNA" misc_feature 654..1403 /label="psi_plus_pack" misc_feature 990..1397 /label="gag" misc_feature 1333..1355 /label="MSCV_primer" misc_feature 1333..1355 /label="pLXSN_5_primer" misc_feature 1371..1387 /label="pBABE_5_primer" CDS complement(1373..2074) /label="ORF frame 2" CDS 1433..4231 /label="ORF frame 2" CDS complement(2246..2797) /label="ORF frame 2" CDS complement(2995..3558) /label="ORF frame 3" CDS complement(3290..4357) /label="ORF frame 2" gene 3494..4198 /label="mCherry" /gene="mCherry" misc_feature 3671..3724 /label="Q66M (mCherry)" misc_feature 4199..4219 /label="SV40 NLS" misc_feature 4205..4219 /label="NLS" misc_feature complement(4285..4308) /label="MSCV_rev_primer" CDS 4530..5369 /label="ORF frame 3" misc_feature 4682..4701 /label="mPGK_F_primer" CDS complement(4695..5420) /label="ORF frame 1" gene 4770..5369 /label="puro (variant)" /gene="puro (variant)" promoter complement(6148..6166) /label="M13_reverse_primer" misc_feature complement(6165..6187) /label="M13_pUC_rev_primer" promoter complement(6201..6230) /label="lac_promoter" gene complement(7313..8173) /label="Ampicillin" /gene="Ampicillin" CDS complement(7313..8173) /label="ORF frame 2" promoter complement(8215..8243) /label="AmpR_promoter" misc_feature complement(8402..8424) /label="pGEX_3_primer" misc_feature complement(8623..8754) /label="lacZ_a" misc_feature 8737..8759 /label="M13_pUC_fwd_primer" misc_feature 8887..8906 /label="pBRrevBam_primer" ORIGIN 1 TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TGCAAGGCAT 61 GGAAAATACA TAACTGAGAA TAGAGAAGTT CAGATCAAGG TTAGGAACAG AGAGACAGCA 121 GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 181 ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC TAGAGAACCA TCAGATGTTT 241 CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT TTGAACTAAC CAATCAGTTC 301 GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC AATAAAAGAG CCCACAACCC 361 CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC GGGTACCCGT ATTCCCAATA 421 AAGCCTCTTG CTGTTTGCAT CCGAATCGTG GACTCGCTGA TCCTTGGGAG GGTCTCCTCA 481 GATTGATTGA CTGCCCACCT CGGGGGTCTT TCATTTGGAG GTTCCACCGA GATTTGGAGA 541 CCCCTGCCCA GGGACCACCG ACCCCCCCGC CGGGAGGTAA GCTGGCCAGC GGTCGTTTCG 601 TGTCTGTCTC TGTCTTTGTG CGTGTTTGTG CCGGCATCTA ATGTTTGCGC CTGCGTCTGT 661 ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG TGGAACTGAC GAGTTCTGAA 721 CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTTGG GGGCCGTTTT TGTGGCCCGA 781 CCTGAGGAAG GGAGTCGATG TGGAATCCGA CCCCGTCAGG ATATGTGGTT CTGGTAGGAG 841 ACGAGAACCT AAAACAGTTC CCGCCTCCGT CTGAATTTTT GCTTTCGGTT TGGAACCGAA 901 GCCGCGCGTC TTGTCTGCTG CAGCGCTGCA GCATCGTTCT GTGTTGTCTC TGTCTGACTG 961 TGTTTCTGTA TTTGTCTGAA AATTAGGGCC AGACTGTTAC CACTCCCTTA AGTTTGACCT 1021 TAGGTCACTG GAAAGATGTC GAGCGGATCG CTCACAACCA GTCGGTAGAT GTCAAGAAGA 1081 GACGTTGGGT TACCTTCTGC TCTGCAGAAT GGCCAACCTT TAACGTCGGA TGGCCGCGAG 1141 ACGGCACCTT TAACCGAGAC CTCATCACCC AGGTTAAGAT CAAGGTCTTT TCACCTGGCC 1201 CGCATGGACA CCCAGACCAG GTCCCCTACA TCGTGACCTG GGAAGCCTTG GCTTTTGACC 1261 CCCCTCCCTG GGTCAAGCCC TTTGTACACC CTAAGCCTCC GCCTCCTCTT CCTCCATCCG 1321 CCCCGTCTCT CCCCCTTGAA CCTCCTCGTT CGACCCCGCC TCGATCCTCC CTTTATCCAG 1381 CCCTCACTCC TTCTCTAGGC GCCGGAATTA GATCTCTCGA GTTGGGCCCA CCATGGTTGC 1441 TAGCAAAGTT CCTGAAGCAG CCATTTCCAG ACTGATTACT TACCTGAGAA TTCTGGAAGA 1501 GCTGGAAGCC CAAGGTGTAC ACCGCACCGC CTCCGAGCAA CTGGGCGAGC TGGCCCAGGT 1561 CACCGCCTTC CAGGTTGATA AGGACCTGTC CTACTTCGGC TCCTACGGCA CTGACGGCGT 1621 TGGCTACACT GTACCGGTCC TCAAGAGAGA ACTGCGCCAT ATCCTCGGTC TGAACCGCAA 1681 ATGGGGCCTG TGCATCGTGG GGATGGGCCG CCTGGGATCC GCTCTTGCTG ATTGGCCTGG 1741 TTTTGGCGAG AGCTTCGAGC TGCGCGGTTT CTTCGATGTT GACCCAGGTA TGGTTGGTCG 1801 GCCGGTTCGC GGTGGTGTTA TCGAACACGT TGATCTGCTG CCCCAGCGCG TACCTGGTCG 1861 CATCGAAATC GCTCTGCTGA CGGTTCCACG CGAGGCAGCA CAGAAGGCTG CCGACCTGCT 1921 GGTTGCCGCT GGCATCAAGG GCATCCTGAA CTTCGCTCCA GTTGTACTGG AGGTTCCCAA 1981 GGAAGTGGCT GTTGAGAACG TTGACATCCT GGCCGGTCTG ACGCGTCTGA GCTTTGCCAT 2041 TCTGAACCCC ACGTGGAGCG CAGCAGGTGG GCATGGTTTC ACTGCTCACA ACGTGTATAT 2101 CATGGCTGAC AAACAGAAGA ACGGTATCAA GGCCAACTTC AAGATCCGCC ACAACATCGA 2161 AGACGGCGGC GTTCAGCTGG CTGATCACTA CCAGCAGAAC ACCCCAATCG GCGACGGCCC 2221 TGTTCTGCTG CCTGACAACC ACTACTTAAG CATCCAGTCT AAACTGTCCA AAGACCCAAA 2281 CGAGAAGCGC GACCACATGG TTCTGCTGGA ATTTGTTACC GCCGCTGGCA TCACCCACGG 2341 CATGGACGAA CTGTACAAGG GCGGCACCGG GGGATCTATG GTTTCCAAAG GCGAGGAACT 2401 GTTCACTGGT GTTGTTCCTA TCCTGGTTGA ACTGGACGGC GACGTTAACG GCCACAAATT 2461 CAGCGTTAGC GGGGAAGGCG AAGGAGACGC TACCTACGGC AAGCTGACTC TGAAGTTCAT 2521 CTGCACCACT GGCAAGCTGC CTGTTCCTTG GCCTACCCTG GTTACTACCT TCTCCTATGG 2581 TGTTATGGTT TTCGCCAGAT ATCCTGACCA CATGAAGCAG CACGACTTCT TCAAGAGCGC 2641 CATGCCTGAG GGCTACGTTC AGGAACGCAC CATCTTTTTC AAGGACGACG GCAACTATAA 2701 GACACGCGCT GAGGTTAAGT TCGAGGGCGA CACTCTGGTT AACCGCATCG AGCTGAAGGG 2761 CATCGACTTC AAGGAGGACG GCAACATCCT GGGCCATAAG CTTGAATATA ACACCAAGGT 2821 ACCTGAAGCC GCCATCAGCC GCCTCATCAC TTACCTCCGC ATCCTGGAGG AGCTCGAAGC 2881 CCAAGGCGTT CACCGCACTG CAAGCGAGCA GCTGGGCGAA CTGGCACAGG TTACAGCATT 2941 CCAGGTTGAC GAAGATCTGT CCTATTTCGG TTCCTACGGC ACCGACGGTG TTGGCTACAC 3001 AGTCCCTGTC CTGAAGCGCG AGCTGCGCCA CATCCTGGGC CTGAACCGCA AGTGGGGCTT 3061 ATGCATCGTT GGCATGGGCC GCCTCGGTAG TGCACTGGCA GACTGGCCGG GCTTTGGTGA 3121 ATCTTTCGAA CTGCGCGGCT TCTTCGACGT TGATCCAGGC ATGGTTGGGC GCCCTGTTCG 3181 CGGTGGCGTT ATCGAGCATG TTGACCTCCT GCCACAGCGC GTCCCGGGTC GCATCGAGAT 3241 CGCCCTGCTG ACCGTTCCTC GCGAAGCTGC ACAGAAGGCA GCCGACTTAC TAGTCGCCGC 3301 TGGTATCAAG GGAATTCTGA ACTTCGCCCC TGTTGTTCTG GAGGTTCCTA AGGAAGTTGC 3361 TGTTGAAAAC GTTGATTTCC TGGCCGGCCT GACCCGCCTG AGCTTCGCAA TCCTGAATCC 3421 TAAGTGGCGC GAAGAAATGA TGGGTTCCGG AACTGGGGGC AACGCCTCTG ACGGTGGTGG 3481 GTCTGGTGGT ATGGTGAGCA AGGGCGAGGA GGATAACATG GCCATCATCA AGGAGTTCAT 3541 GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA 3601 GGGCGAGGGC CGCCCCTACG AGGGCACCCA GACCGCCAAG CTGAAGGTGA CCAAGGGTGG 3661 CCCCCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA 3721 CGTGAAGCAC CCCGCCGACA TCCCCGACTA CTTGAAGCTG TCCTTCCCCG AGGGCTTCAA 3781 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCGTGGTG ACCGTGACCC AGGACTCCTC 3841 CCTGCAGGAC GGCGAGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCTCCGA 3901 CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC TCCTCCGAGC GGATGTACCC 3961 CGAGGACGGC GCCCTGAAGG GCGAGATCAA GCAGAGGCTG AAGCTGAAGG ACGGCGGCCA 4021 CTACGACGCT GAGGTCAAGA CCACCTACAA GGCCAAGAAG CCCGTGCAGC TGCCCGGCGC 4081 CTACAACGTC AACATCAAGT TGGACATCAC CTCCCACAAC GAGGACTACA CCATCGTGGA 4141 ACAGTACGAA CGCGCCGAGG GCCGCCACTC CACCGGCGGC ATGGACGAAC TGTACAAGCC 4201 TAAAAAGAAA CGTAAAGTTG AAGATGCTTG AGTCGACTAC AATTCTACCG GGTAGGGGAG 4261 GCGCTTTTCC CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC 4321 TACACAAGTG GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGGCT 4381 CCGTTCTTTG GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC 4441 CCCGCCCCGC AGCTCGCGTC GTGCAGGACG TGACAAATGG AAGTAGCACG TCTCACTAGT 4501 CTCGTGCAGA TGGACAGCAC CGCTGAGCAA TGGAAGCGGG TAGGCCTTTG GGGCAGCGGC 4561 CAATAGCAGC TTTGCTCCTT CGCTTTCTGG GCTCAGAGGC TGGGAAGGGG TGGGTCCGGG 4621 GGCGGGCTCA GGGGCGGGCT CAGGGGCGGG GCGGGCGCCC GAAGGTCCTC CGGAGGCCCG 4681 GCATTCTGCA CGCTTCAAAA GCGCACGTCT GCCGCGCTGT TCTCCTCTTC CTCATCTCCG 4741 GGCCTTTCGA CCTGCAGCCC AAGCTTACCA TGACCGAGTA CAAGCCCACG GTGCGCCTCG 4801 CCACCCGCGA CGACGTCCCC AGGGCCGTAC GCACCCTCGC CGCCGCGTTC GCCGACTACC 4861 CCGCCACGCG CCACACCGTC GATCCGGACC GCCACATCGA GCGGGTCACC GAGCTGCAAG 4921 AACTCTTCCT CACGCGCGTC GGGCTCGACA TCGGCAAGGT GTGGGTCGCG GACGACGGCG 4981 CCGCGGTGGC GGTCTGGACC ACGCCGGAGA GCGTCGAAGC GGGGGCGGTG TTCGCCGAGA 5041 TCGGCCCGCG CATGGCCGAG TTGAGCGGTT CCCGGCTGGC CGCGCAGCAA CAGATGGAAG 5101 GCCTCCTGGC GCCGCACCGG CCCAAGGAGC CCGCGTGGTT CCTGGCCACC GTCGGCGTCT 5161 CGCCCGACCA CCAGGGCAAG GGTCTGGGCA GCGCCGTCGT GCTCCCCGGA GTGGAGGCGG 5221 CCGAGCGCGC CGGGGTGCCC GCCTTCCTGG AGACCTCCGC GCCCCGCAAC CTCCCCTTCT 5281 ACGAGCGGCT CGGCTTCACC GTCACCGCCG ACGTCGAGGT GCCCGAAGGA CCGCGCACCT 5341 GGTGCATGAC CCGCAAGCCC GGTGCCTGAC GCCCGCCCCA CGACCCGCAG CGCCCGACCG 5401 AAAGGAGCGC ACGACCCCAT GCATCGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA 5461 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT 5521 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA 5581 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA 5641 GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT 5701 CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC 5761 AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC 5821 CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG GGTACCCGTG 5881 TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG 5941 TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT CATGGGTAAC AGTTTCTTGA 6001 AGTTGGAGAA CAACATTCTG AGGGTAGGAG TCGAATATTA AGTAATCCTG ACTCAATTAG 6061 CCACTGTTTT GAATCCACAT ACTCCAATAC TCCTGAAATA GTTCATTATG GACAGCGCAG 6121 AAGAGCTGGG GAGAATTAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 6181 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 6241 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 6301 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 6361 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 6421 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 6481 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 6541 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 6601 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 6661 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 6721 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 6781 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 6841 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 6901 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 6961 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 7021 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 7081 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 7141 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 7201 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 7261 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT 7321 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 7381 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 7441 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 7501 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 7561 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 7621 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 7681 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 7741 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 7801 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 7861 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 7921 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 7981 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 8041 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 8101 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 8161 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC 8221 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA 8281 CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT 8341 ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA 8401 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 8461 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT 8521 ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA 8581 GATGCGTAAG GAGAAAATAC CGCATCAGGC GCCATTCGCC ATTCAGGCTG CGCAACTGTT 8641 GGGAAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA GCTGGCGAAA GGGGGATGTG 8701 CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA GTCACGACGT TGTAAAACGA 8761 CGGCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC 8821 TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC 8881 CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC 8941 GGCCACGATG CGTCCGGCGT AGAGGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT 9001 GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC 9061 ATAGATAAAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAA //