| 质粒名称: | IFP2.0-N1 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 54785 |
| 存储实验室: | Michael Davidson, Xiaokun Shu |
|
载体骨架基本信息 |
|
| 载体名称: | IFP2.0-N1 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | 4989 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | NONE |
| 标签/融合蛋白: | IFP2.0 (C terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: | IFP2.0-N1 was a gift from Michael Davidson & Xiaokun Shu (Addgene plasmid # 54785 ; http://n2t.net/addgene:54785 ; RRID:Addgene_54785) |
| 参考文献部分: | An improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP, Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA, Shu X. Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626. 10.1038/ncomms4626 PubMed 24832154 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS IFP2.0-N1 4978 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: N1 Cloning Vector, Excitation: 684, Emission: 708.
ACCESSION .
VERSION .
KEYWORDS IFP2.0-N1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4978)
AUTHORS Yu D, Gustafson WC, Han C, Lafaye C, Noirclerc-Savoye M, Ge WP,
Thayer DA, Huang H, Kornberg TB, Royant A, Jan LY, Jan YN, Weiss WA,
Shu X
TITLE An improved monomeric infrared fluorescent protein for neuronal and
tumour brain imaging.
JOURNAL Nat Commun. 2014 May 15;5:3626. doi: 10.1038/ncomms4626.
PUBMED 24832154
REFERENCE 2 (bases 1 to 4978)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4978
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
misc_feature 598..678
/label=MCS
/note="multiple cloning site"
regulatory 680..689
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 686..1651
/codon_start=1
/product="bacteriophytochrome-based monomeric infrared
fluorescent protein (Yu et al., 2014)"
/label=IFP2.0
/translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
polyA_signal 1773..1894
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1810..1829)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1864..1883
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1901..2356)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2038..2059)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2250..2269
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2383..2487
/gene="bla"
/label=AmpR promoter
promoter 2489..2846
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2512..2532)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2697..2832
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2759..2778
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2881..3675
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2935..2954)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3545..3564
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3863..3882)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3907..3954
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4283..4871
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4772..4791
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
661 ggcccgggat ccaccggtcg ccaccatggc tcgggaccct caacctttct tccctcctct
721 gtatctcgga ggaccagaga tcactactga gaactgcgag cgggaaccta tacatatacc
781 aggttctatt caacctcatg gagcactgct cacagccgat ggacattctg gcgaggtgct
841 gcaagtttca ctcaacgcag ccactttcct gggccaggaa ccaactgttc tgcggggaca
901 gaccctggca gccctgctgc cagaccagtg gcctgctctg cagactgccc tgcctccagg
961 ctgccaggac gccctgcagt atcgcgccac cctggattgg cccgccgccg gacacctgag
1021 cctgaccgtg catagggtgg ccgagctgct gattctggaa ttcgagccta ctgaggcatg
1081 ggactctatt ggaccccacg ctctgagaaa tgcaatgttc gctctggaga gtgctcctaa
1141 tctccgggca ctggctgaag tcgcaaccca aacagtccgg gaactgtcag gtttcgaccg
1201 ggtgatgctg tacaagtttg caccagacgc aacaggagag gttattgccg aagcaaggcg
1261 cgagggcatg caggcttacc tcgggcatag gtttcccgca tccaccaccc ctgcacaagc
1321 tagggccctc tacacaagac acctgctccg gctgaccgca gacaccaggg ctgcagcagt
1381 gcccctcgac cccgtgctga atccccagac aaatgctcct acacctctgg gcggagctgt
1441 cctcagagct acatccccaa tgcacatgca gtacctgagg aatatgggag tgggctcctc
1501 cctgagcgtc agcgtcgtgg tcggcggcca gctgtgggga ctgattgtct gccaccatca
1561 gacaccctac gtgctgccac ccgatctgcg gaccaccctg gagtatctgg ggaggctcct
1621 gtccctgcag gtgcagagga aagaagcctg agcggccgcg actctagatc ataatcagcc
1681 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
1741 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
1801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
1861 gttgtggttt gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt
1921 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
1981 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
2041 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
2101 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
2161 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
2221 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
2281 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
2341 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
2401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
2461 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
2521 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
2581 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
2641 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
2701 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
2761 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
2821 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
2881 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
2941 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
3001 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
3061 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
3121 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
3181 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
3241 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
3301 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
3361 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
3421 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
3481 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
3541 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
3601 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
3661 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
3721 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
3781 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
3841 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
3901 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
3961 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
4021 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
4081 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
4141 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
4201 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
4261 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
4321 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
4381 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
4441 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
4501 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
4561 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
4621 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
4681 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
4741 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
4801 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
4861 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
4921 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgc
//
