| 质粒名称: | aMHC-mCherry-Rex-Blasticidin |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 21228 |
| 存储实验室: | Mark Mercola |
|
载体骨架基本信息 |
|
| 载体名称: | SMPU |
| 空载体大小: | 7338 bp |
| 完整质粒大小: | 12807 bp |
| 载体类型: | 慢病毒Lentiviral |
| 筛选标记: | Blasticidin resistanct marker driven by Rex-1 promoter |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | alpha myosin heavy chain promoter/aMHC promoter |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 5740 bp |
| 标签/融合蛋白: | mCherry (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | AgeI (未破坏) |
| 5’测序引物 | CTT GAA GCG CAT GAA CTC - 3' Sequencing only |
|
文献引用方法 |
|
| 材料和方法部分: | aMHC-mCherry-Rex-Blasticidin was a gift from Mark Mercola (Addgene plasmid # 21228 ; http://n2t.net/addgene:21228 ; RRID:Addgene_21228) |
| 参考文献部分: | Lentiviral vectors and protocols for creation of stable hESC lines for fluorescent tracking and drug resistance selection of cardiomyocytes. Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R, Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen HS, Mercola M. PLoS ONE. 2009 . 4(4):e5046. 10.1371/journal.pone.0005046 PubMed 19352491 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS aMHC-mCherry-Rex-Blasticidin 12807 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS aMHC-mCherry-Rex-Blasticidin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12807)
AUTHORS Kita-Matsuo H, Barcova M, Prigozhina N, Salomonis N, Wei K, Jacot
JG, Nelson B, Spiering S, Haverslag R, Kim C, Talantova M, Bajpai R,
Calzolari D, Terskikh A, McCulloch AD, Price JH, Conklin BR, Chen
HS, Mercola M
TITLE Lentiviral vectors and protocols for creation of stable hESC lines
for fluorescent tracking and drug resistance selection of
cardiomyocytes.
JOURNAL PLoS ONE. 2009 . 4(4):e5046.
PUBMED 19352491
REFERENCE 2 (bases 1 to 12807)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12807
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 50..353
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 354..557
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 508..528
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 571..751
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 798..923
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 974..1089
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
primer_bind 1165..1181
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1165..1181
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 6639..7349
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(6784..6802)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 7052..7071
/label=mCherry-F
/note="mCherry, forward primer"
CDS 8089..8487
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 8493..9081
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8546..8566)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8964..8975)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 9440..9473
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 9544..9724
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(9774..9793)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(10007..10026)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 10126..10148
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(10186..10204)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 10272..10376
/gene="bla"
/label=AmpR promoter
CDS 10377..11237
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10595..10614)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 11408..11996
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11897..11916
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 12150..12167
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(12237..12257)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 12255..12552
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
promoter 12353..12563
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 12422..12557
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 12484..12503
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 12673..12807
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 12764..12783
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 gggccccaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
541 tctatataag cagagctggt ttagtgaacc gggtctctct ggttagacca gatctgagcc
601 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
661 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
721 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ctgaaagcga
781 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
841 gaggcgaggg gcggcgactg cagagtacgc caaaaatttt gactagcgga ggctagaagg
901 agagagatgg gtgcgagagc gtcagtatta agcgggggaa aataagcccg ggatggcagt
961 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
1021 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
1081 tcaaattttc gggtttatta cagggacagc ggcctatggg aatgaaagac cccacctgtc
1141 gagctccacc gcggtggcgg ccgctctaga actagtggat cctgcaaggt cacacaaggg
1201 tctccaccca ccaggtgccc tagtctcaat ttcagtttcc atgccttgtt ctcacaatgc
1261 tggcctcccc agagctaatt tggactttgt ttttatttca aaagggcctg aatgaggagt
1321 agatcttgtg ctacccagct ctaagggtgc ccgtgaagcc ctcagacctg gagcctttgc
1381 aacagccctt taggtggaag cagaataaag caattttcct taaagccaaa atcctgcctc
1441 tagactcttc ttctctgacc tcggtccctg ggctctaggg tggggaggtg gggcttggaa
1501 gaagaaggtg gggaagtggc aaaagccgat ccctagggcc ctgtgaagtt cggagccttc
1561 cctgtacagc actggctcat agatcctcct ccagccaaac atagcaagaa gtgatacctc
1621 ctttgtgact tccccaggcc cagtacctgt caggttgaaa caggatttag agaagcctct
1681 gaactcacct gaactctgaa gctcatccac caagcaagca cctaggtgcc actgctagtt
1741 agtatcctac gctgataata tgcagagctg ggccacagaa gtcctggggt gtaggaactg
1801 accagtgact tttcagtcgg caaaggtatg accccctcag cagatgtagt aatgtcccct
1861 tagatcccat cccaggcagg tctctaagag gacatgggat gagagatgta gtcatgtggc
1921 attccaaaca cagctatcca cagtgtccct tgccccttcc acttagccag gaggacagta
1981 accttagcct atctttcttc ctccccatcc tcccaggaca caccccctgg tctgcagtat
2041 tcatttcttc cttcacgtcc cctctgtgac ttccatttgc aaggcttttg acctctgcag
2101 ctgctggaag atagagtttg gccctaggtg tggcaagcca tctcaagaga aagcagacaa
2161 cagggggacc agattttgga aggatcagga actaaatcac tggcgggcct gggggtagaa
2221 aaaagagtga gtgagtccgc tccagctaag ccaagctagt ccccgagata ctctgccaca
2281 gctgggctgc tcggggtagc tttaggaatg tgggtctgaa agacaatggg attggaagac
2341 atctctttga gtctcccctc aaccccacct acagacacac tcgtgtgtgg ccagactcct
2401 gttcaacagc cctctgtgtt ctgaccactg agctaggcaa ccagagcatg ggccctgtgc
2461 tgaggatgaa gagttggtta ccaatagcaa aaacagcagg ggagggagaa cagagaacga
2521 aataaggaag gaagaaggaa aggccagtca atcagatgca gtcagaagag atgggaagcc
2581 aacacacagc ttgagcagag gaaacagaaa agggagagat tctgggcata aggaggccac
2641 agaaagaaga gcccaggccc cccaagtctc ctctttatac cctcatcccg tctcccaatt
2701 aagcccactc ttcttcctag atcagacctg agctgcagcg aagagacccg tagggaggat
2761 cacactggat gaaggagatg tgtggagaag tccagggaac ctaagagcca gagcctaaaa
2821 gagcaagaga taaaggtgct tcaaaggtgg ccaggctgtg cacacagagg gtcgaggact
2881 ggtggtagag cctcaagata aggatgatgc tcagaatggg cggggggggg gattctgggg
2941 gggggagaga gaaggtgaga aggagcctgg aacagagaat ctggaagcgc tggaaacgat
3001 accataaagg gaagaaccca ggctaccttt agatgtaaat catgaaagac agggagaagg
3061 gaagctggag agagtagaag gaccccgggg caagacatgg aagcaaggac aagccaggtt
3121 gagcgctccg tgaaatcagc ctgctgaagg cagagccctg gtatgagcac cagaacagca
3181 gaggctaggg ttaatgtcga gacagggaac agaaggtaga cacaggaaca gacagagacg
3241 ggggagccag gtaacaaagg aatggtcctt ctcacctgtg gccagagcgt ccatctgtgt
3301 ccacatactc tagaatgttc atcagactgc agggctggct tgggaggcag ctggaaagag
3361 tatgtgagag ccaggggaga caagggggcc taggaaagga agaagagggc aaaccaggcc
3421 acacaagagg gcagagccca gaactgagtt aactccttcc ttgttgcatc ttccatagga
3481 ggcagtggga actctgtgac caccatcccc catgagcccc cactacccat accaagtttg
3541 gcctgagtgg cattctaggt tccctgagga cagagcctgg cctttgtctc ttggacctga
3601 cccaagctga cccaatgttc tcagtacctt atcatgccct caagagcttg agaaccaggc
3661 agtgacatat taggccatgg gctaaccctg gagcttgcac acaggagcct caagtgacct
3721 ccagggacac agctgcagac aggtggcctt tatccccaaa gagcaaccat ttggcatagg
3781 tggctgcaaa tgggaatgca aggttgaatc aggtcccttc aagaatactg catgcaagac
3841 ctaagacccc tggagagagg ggtatgctcc tgcccccacc caccataagg ggagtgaact
3901 atcctagggg gctggcgacc ttggggagac accacattac tgagagtgct gagcccagaa
3961 aaactgaccg ccctgtgtcc tgcccacctc cacactctag agctatattg agaggtgaca
4021 gtagataggg tgggagctgg tagcagggag agtgttcctg ggtgtgaggg tgtaggggaa
4081 agccagagca ggggagtctg gctttgtctc ctgaacacaa tgtctactta gttataacag
4141 gcatgacctg ctaaagaccc aacatctacg acctctgaaa agacagcagc cctggaggac
4201 aggggttgtc tctgagcctt gggtgcttga tggtgccaca aaggagggca tgagtgtgag
4261 tataaggccc caggagcgtt agagaagggc acttgggaag gggtcagtct gcagagcccc
4321 tatccatgga atctggagcc tggggccaac tggtgtaaat ctctgggcct gccaggcatt
4381 caaagcagca cctgcatcct ctggcagcct ggggaggcgg aagggagcaa ccccccactt
4441 ataccctttc tccctcagcc ccaggattaa cacctctggc cttccccctt cccacctccc
4501 atcaggagtg gagggttgca gagggagggt aaaaacctac atgtccaaac atcatggtgc
4561 acgatatatg gatcagtatg tgtagaggca agaaaggaaa tctgcaggct taactgggtt
4621 aatgtgtaaa gtctgtgtgc atgtgtgtgt gtctgactga aaacgggcat ggctgtgcag
4681 ctgttcagtt ctgtgcgtga ggttaccaga ctgcaggttt gtgtgtaaat tgcccaaggc
4741 aaagtgggtg aatcccttcc atggtttaaa gagattggat gatggcctgc atctcaagga
4801 ccatggaaaa tagaatggac actctatatg tgtctctaag ctaaggtagc aaggtctttg
4861 gaggacacct gtctagagat gtgggcaaca gagactacag acagtatctg tacagagtaa
4921 ggagagagag gagggggtgt agaattctct tactatcaaa gggaaactga gtcgtgcacc
4981 tgcaaagtgg atgctctccc tagacatcat gactttgtct ctggggagcc agcactgtgg
5041 aacttcaggt ctgagagagt aggaggctcc cctcagcctg aagctatgca gatagccagg
5101 gttgaaaggg ggaagggaga gcctgggatg ggagcttgtg tgttggaggc aggggacaga
5161 tattaagcct ggaagagaag gtgaccctta cccagttgtt caactcaccc ttcagattaa
5221 aaataactga ggtaagggcc tgggtagggg aggtggtgtg agacgctcct gtctctcctc
5281 tatctgccca tcggcccttt ggggaggagg aatgtgccca aggactaaaa aaaggccatg
5341 gagccagagg ggcgagggca acagaccttt catgggcaaa ccttggggcc ctgctgtcct
5401 cctgtcacct ccagagccaa gggatcaaag gaggaggagc caggacagga gggaagtggg
5461 agggagggtc ccagcagagg actccaaatt taggcagcag gcatatggga tgggatataa
5521 aggggctgga gcactgagag ctgtcagaga tttctccaac ccaggtaaga gggagtttcg
5581 ggtgggggct cttcacccac accagacctc tccccaccta gaaggaaact gcctttcctg
5641 gaagtggggt tcaggccggt cagagatctg acagggtggc cttccaccag cctgggaagt
5701 tctcagtggc aggaggtttc cacaagaaac actggatgcc ccttccctta cgctgtcttc
5761 tccatcttcc tcctggggat gctcctcccc gtcttggttt atcttggctc ttcgtcttca
5821 gcaagatttg ccctgtgctg tccactccat ctttctctac tgtctccgtg ccttgccttg
5881 ccttcttgcg tgtccttcct ttccacccat ttctcacttc accttttctc cccttctcat
5941 ttgtattcat ccttccttcc ttccttcctt ccttccttcc ttccttcctt ccttcctttc
6001 tcccttcctt ccttccttcc ttccttcctt ccttccttcc ttcctgtgtc agagtgctga
6061 gaatcacacc tggggttccc acccttatgt aaacaatctt ccagtgagcc acagcttcag
6121 tgctgctggg tgctctctta ccttcctcac cccctggctt gtcctgttcc atcctggtca
6181 ggatctctag attggtctcc cagcctctgc tactcctctt cctgcctgtt cctctctctg
6241 tccagctgcg ccactgtggt gcctcgttcc agctgtggtc cacattcttc aggattctct
6301 gaaaagttaa ccaggtgaga atgtttcccc tgtagacagc agatcacgat tctcccggaa
6361 gtcaggcttc cagccctctc tttctctgcc cagctgcccg gcactcttag caaacctcag
6421 gcacccttac cccacataga cctctgacag agaagcaggc actttacatg gagtcctggt
6481 gggagagcca taggctacgg tgtaaaagag gcagggaagt ggtggtgtag gaaagtcagg
6541 acttcacata gaagcctagc ccacaccaga aatgacagac agatccctcc tatctccccc
6601 ataagagttt gagtcgactg atcaaaaacc ggtccaccat ggtgagcaag ggcgaggagg
6661 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
6721 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
6781 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
6841 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
6901 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
6961 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
7021 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
7081 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
7141 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
7201 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
7261 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
7321 ccggcggcat ggacgagctg tacaagtaag gcgcgccacc gattcctccc gataagtaaa
7381 ttacagaaaa gcttcaggtt aaatgtaaga ggatcttaaa tattgatttg gtttttgtct
7441 taatggtgcc cggcgtattt ataagaacca ttaaatttca ctagaccgtt tgtagtcagg
7501 ggtgaacgct gcatgaccca cgctctcaaa gtgagttgtg ttgctttgcg ggaatccagc
7561 agtcacatta atgctggaaa aagttcaggc aactagtgta ctttgtagcg gggtccggga
7621 gaggctgggg tctagagtgg cgatgggacg aaagggtaaa gaaagttttc ggtgtgtgtg
7681 tgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgtgtagg taggtaggta ggtagaaact
7741 gcatcctctg cttgtgtaaa tccggttaca tgtgtgtaac agaggtactg agatgtgact
7801 gagtctcaag gccaggcgat cgggattcag aagaggcatt tgcataactg agcaagagcc
7861 tttgccccac ccttccacgc ggacccaaga caagcggaca gctctgggtg ggtcaccttg
7921 aagccagggg cccgcccaca tccccgccca cacccacctt gagcgcttct cattgggcat
7981 tccagcctac ccagctcgga gttagttact ccgtaagtgt ggccggaaca gagttcgtcc
8041 atctaaaaag ggaggggacc ggcgaacaca tccggtagga gggccatcat ggccaagcct
8101 ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc
8161 atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact
8221 ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact
8281 gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg
8341 ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc
8401 aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg
8461 ccctctggtt atgtgtggga gggctaacta gaaatcaacc tctggattac aaaatttgtg
8521 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
8581 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
8641 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
8701 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
8761 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
8821 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
8881 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
8941 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
9001 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
9061 ccctttgggc cgcctccccg cctggtcgac aggagctttg ttccttgggt tcttgggagc
9121 agcaggaagc actatgggct gcacgtcaat gacgctgacg gtacaggcca gacaattatt
9181 gtctgatata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
9241 gttgcaactc acagtctggg gcatcaaaca gctccaggca agaatcctgg ctgtggaaag
9301 atacctaaag gatcaacagc tcctggtacc tttaagacca atgacttaca aggcagctgt
9361 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
9421 aagacaagat atcctcgaga taacttcgta tagcatacat tatacgaagt tatcagtcga
9481 gtttatttgt gaaatttgtg atgctattgc tttatttgta agcagctgct ttttgcctgt
9541 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
9601 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
9661 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
9721 agcagccaaa cgcgtccgcg gtgtacccgg cgccacaggt gcggttgctg gcgcctatat
9781 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
9841 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
9901 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
9961 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
10021 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
10081 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
10141 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
10201 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
10261 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
10321 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
10381 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
10441 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
10501 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
10561 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
10621 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
10681 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
10741 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
10801 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
10861 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
10921 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
10981 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
11041 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
11101 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
11161 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
11221 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
11281 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
11341 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
11401 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
11461 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
11521 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
11581 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
11641 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
11701 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
11761 gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc
11821 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
11881 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
11941 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
12001 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
12061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
12121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
12181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
12241 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
12301 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
12361 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
12421 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
12481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
12541 aggctttttt ggaggcctag gctagagatc ataatcagcc ataccacatt tgtagaggtt
12601 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
12661 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
12721 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
12781 atcaatgtat cttatcatgt ctggatc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：IFP2.0-N1
- 下一篇：pLenti-GFP1-9
