质粒名称: | pSBY2_ligD(FnCpf1) |
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出品公司: | Addgene |
目录编号: | 104623 |
存储实验室: | Sheng Yang |
载体骨架基本信息 |
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载体名称: | pMN016 |
完整质粒大小: | 6043 bp |
载体类型: | Bacterial Expression, CRISPR |
载体抗性: | Hygromycin |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | crRNA to ligD |
物种来源: | Synthetic |
启动子: | Pj23119 |
克隆方法: | Gibson Cloning |
文献引用方法 |
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材料和方法部分: | pSBY2_ligD(FnCpf1) was a gift from Sheng Yang (Addgene plasmid # 104623 ; http://n2t.net/addgene:104623 ; RRID:Addgene_104623) |
参考文献部分: |
pSBY2_ligD(FnCpf1) was a gift from Sheng Yang (Addgene plasmid # 104623 ; http://n2t.net/addgene:104623 ; RRID:Addgene_104623)
For your References section:
A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y. Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6. 10.1002/biot.201700588 PubMed 30039929
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSBY2_ligD(FnCpf1) 6043 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION CRISPR system used to genome editing in Mycobacterium smegmatis, expresses crRNA to ligD. ACCESSION . VERSION . KEYWORDS pSBY2_ligD(FnCpf1) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6043) AUTHORS Sun B, Yang J, Yang S, Ye RD, Chen D, Jiang Y TITLE A CRISPR-Cpf1-Assisted Non-Homologous End Joining Genome Editing System of Mycobacterium smegmatis. JOURNAL Biotechnol J. 2018 Sep;13(9):e1700588. doi: 10.1002/biot.201700588. Epub 2018 Aug 6. PUBMED 30039929 REFERENCE 2 (bases 1 to 6043) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..6043 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(727..743) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(728..744) /label=pBluescriptKS /note="For pBluescript vector" rep_origin 4818..5406 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5307..5326 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5560..5577 /label=L4440 /note="L4440 vector, forward primer" terminator 5667..5694 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 5814..5899 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 5991..6018 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" ORIGIN 1 attaagggag aacatgaagg caatcagtcg ggtgctgatc gcgatggttg cagccatcgc 61 ggcgcttttc acgagcacag gcacctctca cgcaggcctg gacaacgagc tgagcctcgt 121 tgatggccag gaccgcaccc tcaccgtgca gcagtgggac accttcctca atggtgtgtt 181 ccccctggac cgcaaccgtc ttacccgtga gtggttccac tccggtcgcg ccaagtacat 241 cgtggccggc cccggtgccg acgagttcga gggcacgctg gaactcggct accagatcgg 301 cttcccgtgg tcgctgggtg tgggcatcaa cttcagctac accaccccga acatcctgat 361 cgacgacggt gacatcatcg ctccgccgtt cggcctgaac tcggtcatca ccccgaacct 421 gttccccggt gtgtcgatct cggcagatct gggcaacggc cccggcatcc aggaagtcgc 481 aacgttctcg gtcgacgtct ccggcgccga gggtggcgtg gccgtgtcga acgcccacgg 541 caccgtgacc ggtgcggccg gcggtgtgct gctgcgtccg ttcgcccgcc tgatcgcctc 601 gaccggtgac tcggtcacca cctacggcga accctggaac atgaactgat tcctggaccg 661 ccgttcgatt taaataagct ttagctaatt aattggggac cctagaggtc ccctttttta 721 ttttatcgat accgtcgacc tcgagggggg gcccccgggg atcatcgagc cgagaacgtt 781 atcgaagttg gtcatgtgta atcccctcgt ttgaactttg gattaagcgt agatacaccc 841 ttggacaagc cagttggatt cggagacaag caaattcagc cttaaaaagg gcgaggccct 901 gcggtggtgg aacaccgcag ggcctctaac cgctcgacgc gctgcaccaa ccagcccgcg 961 aacggctggc agccagcgta aggcgcggct catcgggcgg cgttcgccac gatgtcctgc 1021 acttcgagcc aagcctcgaa cacctgctgg tgtgcacgac tcacccggtt gttgacaccg 1081 cgcgcggccg tgcgggctcg gtggggcggc tctgtcgccc ttgccagcgt gagtagcgcg 1141 tacctcacct cgcccaacag gtcgcacaca gccgattcgt acgccataaa gccaggtgag 1201 cccaccagct ccgtaagttc gggcgctgtg tggctcgtac ccgcgcattc aggcggcagg 1261 gggtctaacg ggtctaaggc ggcgtgtacg gccgccacag cggctctcag cggcccggaa 1321 acgtcctcga aacgacgcat gtgttcctcc tggttggtac aggtggttgg gggtgctcgg 1381 ctgtcgctgg tgttccacca ccagggctcg acgggagagc gggggagtgt gcagttgtgg 1441 ggtggcccct cagcgaaata tctgacttgg agctcgtgtc ggaccataca ccggtgatta 1501 atcgtggtct actaccaagc gtgagccacg tcgccgacga atttgagcag ctctggctgc 1561 cgtactggcc gctggcaagc gacgatctgc tcgaggggat ctaccgccaa agccgcgcgt 1621 cggccctagg ccgccggtac atcgaggcga acccaacagc gctggcaaac ctgctggtcg 1681 tggacgtaga ccatccagac gcagcgctcc gagcgctcag cgcccggggg tcccatccgc 1741 tgcccaacgc gatcgtgggc aatcgcgcca acggccacgc acacgcagtg tgggcactca 1801 acgcccctgt tccacgcacc gaatacgcgc ggcgtaagcc gctcgcatac atggcggcgt 1861 gcgccgaagg ccttcggcgc gccgtcgacg gcgaccgcag ttactcaggc ctcatgacca 1921 aaaaccccgg ccacatcgcc tgggaaacgg aatggctcca ctcagatctc tacacactca 1981 gccacatcga ggccgagctc ggcgcgaaca tgccaccgcc gcgctggcgt cagcagacca 2041 cgtacaaagc ggctccgacg ccgctagggc ggaattgcgc actgttcgat tccgtcaggt 2101 tgtgggccta tcgtcccgcc ctcatgcgga tctacctgcc gacccggaac gtggacggac 2161 tcggccgcgc gatctatgcc gagtgccacg cgcgaaacgc cgaattcccg tgcaacgacg 2221 tgtgtcccgg accgctaccg gacagcgagg tccgcgccat cgccaacagc atttggcgtt 2281 ggatcacaac caagtcgcgc atttgggcgg acgggatcgt ggtctacgag gccacactca 2341 gtgcgcgcca gtcggccatc tcgcggaagg gcgcagcagc gcgcacggcg gcgagcacag 2401 ttgcgcggcg cgcaaagtcc gcgtcagcca tggaggcatt gctatgagcg acggctacag 2461 cgacggctac aaccggcagc cgactgtccg caaaaagcgg cgcgtgaccg ccgccgaagg 2521 cgctcgaatc accggactat ccgaacgcca cgtcgtccgg ctcgtggcgc aggaacgcag 2581 cgagtggctc gccgagcagg ctgcacgccg cgaacgcatc cgcgcctatc acgacgacga 2641 gggccactct tggccgcaaa cggccaaaca tttcgggctg catctggaca ccgttaagcg 2701 actcggctat cgggcgagga aagagcgtgc ggcagaacag gaagcggctc aaaaggccca 2761 caacgaagcc gacaatccac cgctgttcta acgcaattgg ggagcgggtg tcgcgggggt 2821 tccgtggggg gttccgttgc aacgggtcgg acaggtaaaa gtcctggtag acgctagttt 2881 tctggtttgg gccatgcctg tctcgttgcg tgtttcgttg cgcccgtttt gaataccagc 2941 cagacgagac ggggttctac gaatcttggt cgataccaag ccatttccgc tgaatatcgg 3001 ggagctcacc gccagaatcg gtggttgtgg tgatgtacgt ggcgaactcc gttgtagtgc 3061 ctgtggtggc atccgtggcc actctcgttg cacggttcgt tgtgccgtta caggccccgt 3121 tgacagctca ccgaacgtag ttaaaacatg ctggtcaaac taggtttacc aacgatacga 3181 gtcagctcat ctagggccag ttctaggcgt tgttcgttgc gcggttcgtt gcgcatgttt 3241 cgtgtggttg ctagatggct ccgcaaccac acgcttcgag gttgagtgct tccagcacgg 3301 gcgcgatcca gaagaacttc gtcgtgcgac tgtcctcgtt gatccttgcc gagctgggat 3361 ggaagcccgg ccgacccacc ctggaggaga tgatcgagga tgccagggcc tttcacgccc 3421 gccgctgctg agcgtccgcc gccgggcggc ccggggcgtc aggcgccggg ggcggtgtcc 3481 ggcggccccc agaggaactg cgccagttcc tccggatcgg tgaagccgga gagatccagc 3541 ggggtctcct cgaacacctc gaagtcgtgc aggaaggtga aggcgagcag ttcgcgggcg 3601 aagtcctcgg tccgcttcca ctgcgccccg tcgagcagcg cggccaggat ctcgcggtcg 3661 ccccggaagg cgttgagatg cagttgcacc aggctgtagc gggagtctcc cgcatagacg 3721 tcggtgaagt cgacgatccc ggtgacctcg gtcgcggcca ggtccacgaa gatgttggtc 3781 ccgtgcaggt cgccgtggac gaaccggggt tcgcggccgg ccagcagcgt gtccacgtcc 3841 ggcagccagt cctccaggcg gtccagcagc cggggcgaga ggtagcccca cccgcggtgg 3901 tcctcgacgg tcgccgcgcg gcgttcccgc agcagttccg ggaagacctc ggaatggggg 3961 gtgagcacgg tgttcccggt cagcggcacc ctgtgcagcc ggccgagcac ccggccgagt 4021 tcgcgggcca gggcgagcag cgcgttccgg tcggtcgtgc cgtccatcgc ggaccgccag 4081 gtggtgccgg tcatccggct catcaccagg tagggccacg gccaggctcc ggtgccgggc 4141 cgcagctcgc cgcggccgag gaggcggggc accggcaccg gggcgtccgc caggaccgcg 4201 tacgcctccg actccgacgc gaggctctcc ggaccgcacc agtgctcgcc gaacagcttg 4261 atcaccgggc cgggctcgcc gaccagtacg gggttggtgc tctcgccggg cacccgcagc 4321 accggcggca ccggcagccc gagctcctcc agggctcggc gggccagcgg ctcccagaat 4381 tcctggtcgt tccgcaggct cgcgtaggaa tcatccgaat caatacggtc gagaagtaac 4441 agggattctt gtgtcacagc ggacctctat tcacagggta cgggccggct taattccgca 4501 cggccggtcg cgacacggcc tgtccgcacc gcggatcagg cgttgacgat gacgggctgg 4561 tcggccacgt cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 4621 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 4681 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 4741 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 4801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 4861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 4921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg 4981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 5041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 5101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 5161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 5221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 5281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 5341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 5401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 5461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 5521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa 5581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 5641 agctagaact agttgattag ctaagcagaa ggccatcctg acggatggcc tttttgcgtt 5701 taatactgtt taaactctag actcgagtct agattgacag ctagctcagt cctaggtata 5761 atggatccga atttctactg ttgtagatag cacgtcacac gcaacggtga tttaaataaa 5821 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 5881 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg 5941 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat 6001 cctgacggat ggcctttttg cgtttctaca aactcttaag ctt //
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