买家导航
pcDNA3.1+/C-HA载体质粒基本信息
pcDNA3.1+/C-HA质粒图谱载体图谱和pcDNA3.1+/C-HA载体序列质粒序列多克隆位点信息
pcDNA3.1+/C-HA质粒载体简介
pcDNA3.1+/C-HA质粒序列载体序列
LOCUS pcDNA3.1+ C-HA 5441 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian expression vector with a CMV promoter for expressing
C-terminally HA-tagged proteins.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ C-HA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5441)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5441
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 895..985
/note="MCS"
/note="multiple cloning site"
CDS 986..1012
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
polyA_signal 1038..1262
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1308..1736
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1750..2079
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1930..2065
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2146..2940
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3114..3235
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3686..4274)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4445..5305)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5306..5410)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
961 gcggccgctc gagtctagag ggccctaccc atacgatgtt ccagattacg cttgataaac
1021 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
1081 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
1141 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
1201 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
1261 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
1321 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
1381 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
1441 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
1501 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
1561 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
1621 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
1681 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
1741 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
1801 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
1861 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
1921 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1981 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
2041 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
2101 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
2161 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
2221 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
2281 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
2341 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
2401 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
2461 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
2521 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
2581 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
2641 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
2701 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
2761 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
2821 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
2881 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
2941 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
3001 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
3061 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
3121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
3181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
3241 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
3301 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
3361 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
3421 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
3481 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
3541 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
3601 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
3661 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
3721 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
3781 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
3841 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
3901 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3961 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
4021 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
4081 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
4141 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4201 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4261 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4321 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
4381 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
4441 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
4501 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
4561 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
4621 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
4681 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
4741 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
4801 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
4861 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
4921 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
4981 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
5041 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
5101 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
5161 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
5221 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
5281 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
5341 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5401 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
pcDNA3.1+/C-HA载体图谱质粒图谱pdf版和pcDNA3.1+/C-HA载体序列质粒序列等相关资料下载
pcDNA3.1+/C-HA质粒载体应用举例
- 上一篇：pcDNA3.1+/C-DYK
- 下一篇：pcDNA3.1+/C-Myc
