| 质粒名称: | pTRKH3-ermGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27169 |
| 存储实验室: | Michela Lizier |
|
载体骨架基本信息 |
|
| 载体名称: | pTRKH3 |
| 载体出品公司: | -- |
| 空载体大小: | 7493 bp |
| 完整质粒大小: | 8961 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 红霉素，200μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 红霉素核糖体甲基化酶启动子区与修饰的绿色荧光蛋白5的融合 |
| 物种来源: | 粪肠球菌及其合成构建体 |
| 插入大小: | 1279 bp |
| 基因突变: | 在插入物的N末端存在额外的183bp区域 |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SalI (未破坏) |
| 3’克隆位点: | BamHI(未破坏) |
| 5’测序引物 | AGTGAAAAGTTCTTCTCCTT |
| 3’测序引物 | GCGGCACGACTTCTTCAAGAGC |
|
文献引用方法 |
|
| 材料和方法部分: | pTRKH3-ermGFP was a gift from Michela Lizier (Addgene plasmid # 27169 ; http://n2t.net/addgene:27169 ; RRID:Addgene_27169) |
| 参考文献部分: | Comparison of expression vectors in Lactobacillus reuteri strains. Lizier M, Sarra PG, Cauda R, Lucchini F. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15. 10.1111/j.1574-6968.2010.01978.x PubMed 20455948 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRKH3-ermGFP 8961 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTRKH3-ermGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8961)
AUTHORS Lizier M, Sarra PG, Cauda R, Lucchini F
TITLE Comparison of expression vectors in Lactobacillus reuteri strains.
JOURNAL FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
PUBMED 20455948
REFERENCE 2 (bases 1 to 8961)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8961
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 5943..5962
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(6029..6131)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(6523..6540)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6657..7202)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(6737..6756)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 7314..7342
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(7685..8401)
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/label=mgfp5
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(7728..7748)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 8343..8371
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
1 tcgacctgct gacgaccggc gtcgatattc cgtcgatctg taatatcgtg ttcctgcgta
61 aagtacgcag ccgcattctg tacgaacaga tgaaaggccg cgccacgcgc ttatgcccgg
121 aggtgaataa aaccagcttt aagatttttg actgtgtcga tatctacagc acgctggaga
181 gcgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
241 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
301 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
361 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
421 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
481 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
541 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
601 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
661 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
721 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg
781 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc
841 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
901 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg
961 gtcgagtcta gaatcgatac gattttgaag tggcaacaga taaaaaaaag cagtttaaaa
1021 ttgttgctga acttttaaaa caagcaaata caatcattgt cgcaacagat agcgacagag
1081 aaggcgaaaa cattgcctgg tcgatcattc ataaagcaaa tgccttttct aaagataaaa
1141 cgtataaaag actatggatc aatagtttag aaaaagatgt gatccgtagc ggttttcaaa
1201 atttgcaacc aggaatgaat tactatccct tttatcaaga agcgcacaaa aagaaaaacg
1261 aaatgataca ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg
1321 tgctgacttg caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga
1381 agttatggaa ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa
1441 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
1501 gtgattacat gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac
1561 tcaaccaaat aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa
1621 caggtaaagg gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg
1681 aattagacag tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca
1741 ctttaattca ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg
1801 ttgggagtat tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc
1861 atgcgtctga catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc
1921 accgaacact agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc
1981 cagcggaatg ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc
2041 ataccacaga tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg
2101 tcaatcgaga atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg
2161 ccaaagtaaa caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt
2221 atctattatt taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta
2281 cacgttacta aagggaatgt agataaatta ttaggtatac tactgacagc ttccaaggag
2341 ctaaagaggt ccctagcgct cttatcatgg ggaagctcgg atcatatgca agacaaaata
2401 aactcgcaac agcacttgga gaaatgggac gaatcgagaa aaccctcttt acgctggatt
2461 acatatctaa taaagccgta aggagacggg ttcaaaaagg tttaaataaa ggagaagcaa
2521 tcaatgcatt agctagaatt atattttttg gacaacgtgg agaatttaga gaacgtgctc
2581 tccaagacca gttacaaaga gctagtgcac taaacataat tattaacgct ataagtgtgt
2641 ggaacactgt atatatggaa aaagccgtag aagaattaaa agcaagagga gaatttagag
2701 aagatttaat gccatatgcg tggccgttag gatgggaaca tatcaatttt cttggagaat
2761 acaaatttga aggattacat gacactgggc aaatgaattt acgtccttta cgtataaaag
2821 agccgtttta ttcttaatat aacggctctt tttatagaaa aaatccttag cgtggttttt
2881 ttccgaaatg ctggcggtac cccaagaatt agaaatgagt agatcaaatt attcacgaat
2941 agaatcagga aaatcagatc caaccataaa aacactagaa caaattgcaa agttaactaa
3001 ctcaacgcta gtagtggatt taatcccaaa tgagccaaca gaaccagaac cagaaacaga
3061 atcagaacaa gtaacattgg atttagaaat ggaagaagaa aaaagcaatg acttcgtgtg
3121 aataatgcac gaaatcgttg cttatttttt ttaaaagcgg tatactagat ataacgaaac
3181 aacgaactga atagaaacga aaaaagagcc atgacacatt tataaaatgt ttgacgacat
3241 tttataaatg catagcccga taagattgcc aaaccaacgc ttatcagtta gtcagatgaa
3301 ctcttccctc gtaagaagtt atttaattaa ctttgtttga agacggtata taaccgtact
3361 atcattatat agggaaatca gagagttttc aagtatctaa gctactgaat ttaagaattg
3421 ttaagcaatc aatcggaaat cgtttgattg ctttttttgt attcatttat agaaggtgga
3481 gtttgtatga atcatgatga atgtaaaact tatataaaaa atagtttatt ggagataaga
3541 aaattagcaa atatctatac actagaaacg tttaagaaag agttagaaaa gagaaatatc
3601 tacttagaaa caaaatcaga taagtatttt tcttcggagg gggaagatta tatatataag
3661 ttaatagaaa ataacaaaat aatttattcg attagtggaa aaaaattgac ttataaagga
3721 aaaaaatctt tttcaaaaca tgcaatattg aaacagttga atgaaaaagc aaaccaagtt
3781 aattaaacaa cctattttat aggatttata ggaaaggaga acagctgaat gaatatccct
3841 tttgttgtag aaactgtgct tcatgacggc ttgttaaagt acaaatttaa aaatagtaaa
3901 attcgctcaa tcactaccaa gccaggtaaa agcaaagggg ctatttttgc gtatcgctca
3961 aaatcaagca tgattggcgg tcgtggtgtt gttctgactt ccgaggaagc gattcaagaa
4021 aatcaagata catttacaca ttggacaccc aacgtttatc gttatggaac gtatgcagac
4081 gaaaaccgtt catacacgaa aggacattct gaaaacaatt taagacaaat caataccttc
4141 tttattgatt ttgatattca cacggcaaaa gaaactattt cagcaagcga tattttaaca
4201 accgctattg atttaggttt tatgcctact atgattatca aatctgataa aggttatcaa
4261 gcatattttg ttttagaaac gccagtctat gtgacttcaa aatcagaatt taaatctgtc
4321 aaagcagcca aaataatttc gcaaaatatc cgagaatatt ttggaaagtc tttgccagtt
4381 gatctaacgt gtaatcattt tggtattgct cgcataccaa gaacggacaa tgtagaattt
4441 tttgatccta attaccgtta ttctttcaaa gaatggcaag attggtcttt caaacaaaca
4501 gataataagg gctttactcg ttcaagtcta acggttttaa gcggtacaga aggcaaaaaa
4561 caagtagatg aaccctggtt taatctctta ttgcacgaaa cgaaattttc aggagaaaag
4621 ggtttaatag ggcgtaataa cgtcatgttt accctctctt tagcctactt tagttcaggc
4681 tattcaatcg aaacgtgcga atataatatg tttgagttta ataatcgatt agatcaaccc
4741 ttagaagaaa aagaagtaat caaaattgtt agaagtgcct attcagaaaa ctatcaaggg
4801 gctaataggg aatacattac cattctttgc aaagcttggg tatcaagtga tttaaccagt
4861 aaagatttat ttgtccgtca agggtggttt aaattcaaga aaaaaagaag cgaacgtcaa
4921 cgtgttcatt tgtcagaatg gaaagaagat ttaatggctt atattagcga aaaaagcgat
4981 gtatacaagc cttatttagt gacgaccaaa aaagagatta gagaagtgct aggcattcct
5041 gaacggacat tagataaatt gctgaaggta ctgaaggcga atcaggaaat tttctttaag
5101 attaaaccag gaagaaatgg tggcattcaa cttgctagtg ttaaatcatt gttgctatcg
5161 atcattaaag taaaaaaaga agaaaaagaa agctatataa aggcgctgac aaattctttt
5221 gacttagagc atacattcat tcaagagact ttaaacaagc tagcagaacg ccctaaaacg
5281 gacacacaac tcgatttgtt tagctatgat acaggctgaa aataaaaccc gcactatgcc
5341 attacattta tatctatgat acgtgtttgt tttttctttg ctgtttagcg aatgattagc
5401 agaaatatac agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga
5461 aaacttttag ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg
5521 cagtgagagc gaagcgaaca cttgattttt taattttcta tcttttatag gtcattagag
5581 tatacttatt tgtcctataa actatttagc agcataatag atttattgaa taggtcattt
5641 aagttgagca tattagagga ggaaaatctt ggagaaatat ttgaagaacc cgattacatg
5701 gattggatta gttcttgtgg ttacgtggtt tttaactaaa agtagtgaat ttttgatttt
5761 tggtgtgtgt gtcttgttgt tagtatttgc tagtcaaagt gattaaatag aattccggat
5821 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
5881 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
5941 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
6001 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
6061 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
6121 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
6181 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
6241 aaagtgcgtc gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
6301 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
6361 cgtaacggca aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg
6421 ttggcactga tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc
6481 ggtgcgtcag cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct
6541 acgctcggtc gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct
6601 ggaagatgcc aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc
6661 cataggctcc gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga
6721 aacccgacag gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct
6781 cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat
6841 tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga
6901 accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
6961 ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag
7021 tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc
7081 ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc
7141 cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc
7201 aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt
7261 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag
7321 cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag
7381 gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat
7441 gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat
7501 tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt
7561 ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc
7621 gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg
7681 atccttattt gtatagttca tccatgccat gtgtaatccc agcagccgtt acaaactcaa
7741 gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca
7801 ggtaatggtt gtctggtaaa aggacagggt catcgccaat tggagtattt tgttgataat
7861 gatcagcgag ttgcacgccg ccgtcttcga tgttgtggcg ggtcttgaag ttggctttga
7921 tgccgttctt ttgcttgtcg gccatgatgt atacgttgtg ggagttgtag ttgtattcca
7981 acttgtggcc gaggatgttt ccgtcctcct tgaaatcgat tcccttaagc tcgatcctgt
8041 tgacgagggt gtctccctca aacttgactt cagcacgtgt cttgtagttc ccgtcgtcct
8101 tgaagaagat ggtcctctcc tgcacgtatc cctcaggcat ggcgctcttg aagaagtcgt
8161 gccgcttcat atgatctgga tatcttgaaa agcattgaac accataagtg agagtagtga
8221 caagtgttgg ccatggaaca ggtagttttc cagtagtgca aataaattta agggtaagtt
8281 ttccgtatgt tgcatcacct tcaccctctc cactgacaga aaatttgtac ccattaacat
8341 caccatctaa ttcaacaaga attgggacaa ctccagtgaa aagttcttct cctttactca
8401 tgaattcact ccttcttaat tacaaatttt tagcatctaa tttaacttca attcctatta
8461 tacaaaattt taagatactg cactatcaac acactcttaa gtttgcttct aagtcttatt
8521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
8581 aagtcagcac gaacacgaac cgtcttatct cccattatat ctttttttgc actgattggt
8641 gtatcatttc gtttttcttt ttgtgcgctt cttgataaaa gggatagtaa ttcattcctg
8701 gttgcaaatt ttgaaaaccg ctacggatca catctttttc taaactattg atccatagtc
8761 ttttatacgt tttatcttta gaaaaggcat ttgctttatg aatgatcgac caggcaatgt
8821 tttcgccttc tctgtcgcta tctgttgcga caatgattgt atttgcttgt tttaaaagtt
8881 cagcaacaat tttaaactgc ttttttttat ctgttgccac ttcaaaatcg tatcgattct
8941 agactaagct tatcgatacc g
//
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