| 质粒名称: | pLenti-DsRed_IRES_EGFP |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 92194 |
| 存储实验室: | Huda Zoghbi |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-GPS-GAW-IRES |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 9942 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | DsRed-IRES-EGFP |
| 物种来源: | Synthetic |
| 插入大小: | 2000 bp |
|
文献引用方法 |
|
| 材料和方法部分: | pLenti-DsRed_IRES_EGFP was a gift from Huda Zoghbi (Addgene plasmid # 92194 ; http://n2t.net/addgene:92194 ; RRID:Addgene_92194) |
| 参考文献部分: | TRIM28 regulates the nuclear accumulation and toxicity of both alpha-synuclein and tau. Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L, Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY. Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809. 10.7554/eLife.19809 PubMed 27779468 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti-DsRed_IRES_EGFP 9953 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral overexpression vector to make stable bicistronic cell
line for control (EGFP) screen.
ACCESSION .
VERSION .
KEYWORDS pLenti-DsRed_IRES_EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9953)
AUTHORS Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J,
Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
TITLE TRIM28 regulates the nuclear accumulation and toxicity of both
alpha-synuclein and tau.
JOURNAL Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
PUBMED 27779468
REFERENCE 2 (bases 1 to 9953)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9953
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 2..635
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 682..807
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1304..1537
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1722..1766
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2059..2176
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2228..2531
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2532..2735
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2686..2706
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2732..2756
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 2839..2848
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2845..3522
/codon_start=1
/product="rapidly maturing tetrameric variant of DsRed
fluorescent protein (Bevis and Glick, 2002)"
/label=DsRed-Express
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
LFL"
primer_bind complement(2975..2993)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(3025..3045)
/label=DsRed1-N
/note="DsRed1, reverse primer"
primer_bind 3243..3262
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 3437..3460
/label=DsRed1-C
/note="DsRed1, forward primer"
misc_feature 3565..4151
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3732..3749)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3959..3978
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4152..4871
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 4152..4868
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4197..4218)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4458..4477)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4805..4826
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(4891..4910)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4892..4910)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4955..4978)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 5348..5367
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 5436..6230
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5490..5509)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6100..6119
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 6344..6932
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6397..6417)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6815..6826)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7007..7240
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(7359..7377)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7445..7549
/gene="bla"
/label=AmpR promoter
CDS 7550..8410
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7768..7787)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8581..9169
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9070..9089
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9323..9340
/label=L4440
/note="L4440 vector, forward primer"
primer_bind 9432..9451
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
protein_bind 9580..9601
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9616..9646
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9654..9670
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9659..9681
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9678..9694
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9678..9694
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9744..9763
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
2041 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
2101 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
2161 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtgg
2221 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
2281 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
2341 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
2401 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
2461 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
2521 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
2581 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
2641 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
2701 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
2761 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccggcggcct agcgctaccg
2821 gactcagatc caccggtcgc caccatggcc tcctccgagg acgtcatcaa ggagttcatg
2881 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
2941 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
3001 cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc caaggtgtac
3061 gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga gggcttcaag
3121 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
3181 ctgcaggacg gctccttcat ctacaaggtg aagttcatcg gcgtgaactt cccctccgac
3241 ggccccgtaa tgcagaagaa gactatgggc tgggaggcct ccaccgagcg cctgtacccc
3301 cgcgacggcg tgctgaaggg cgagatccac aaggccctga agctgaagga cggcggccac
3361 tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac
3421 tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac catcgtggag
3481 cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt agcggccaat tctgcagtcg
3541 acggtaccgc gggcccggga tccgcccctc tccctccccc ccccctaacg ttactggccg
3601 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
3661 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
3721 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
3781 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
3841 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
3901 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
3961 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
4021 gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa
4081 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
4141 tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
4201 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
4261 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
4321 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
4381 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
4441 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
4501 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
4561 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
4621 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
4681 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
4741 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
4801 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
4861 tgtacaagta atctagataa ctgatcctct ccctatagtg agtcgtatta aattctaccg
4921 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
4981 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
5041 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
5101 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
5161 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
5221 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
5281 tgggtccggg ggcgggctca ggggcgggcg ggcggggcgg gcgcccgaag gtcctccgga
5341 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
5401 tctccgggcc tttcgacctg cagcccaagc ttaccatgat tgaacaagat ggattgcacg
5461 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacga
5521 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
5581 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
5641 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
5701 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
5761 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
5821 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
5881 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
5941 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
6001 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
6061 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
6121 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
6181 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga cctgaggatc
6241 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
6301 cccctgaacc tgaaacataa aatgaatgca attgatagat cctaatcaac ctctggatta
6361 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
6421 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
6481 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
6541 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
6601 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
6661 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
6721 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
6781 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
6841 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
6901 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac
6961 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
7021 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
7081 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
7141 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
7201 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
7261 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta
7321 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
7381 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
7441 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
7501 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
7561 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
7621 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
7681 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
7741 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
7801 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
7861 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
7921 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
7981 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
8041 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
8101 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
8161 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
8221 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
8281 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
8341 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
8401 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
8461 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
8521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
8581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
8641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
8701 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
8761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
8821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
8881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
8941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
9001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
9061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
9121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
9181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
9241 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
9301 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
9361 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
9421 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
9481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
9541 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
9601 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
9661 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
9721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
9781 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
9841 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
9901 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tag
//
