pEF6/V5-His A

2500 2800 3100 3500 询价

价格：元

联系方式：I47-825O-882O

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pEF6/V5-His A载体 pEF6-V5-HisA质粒基本信息

出品公司:	Invitrigen
载体名称:	pEF6/V5-His A
质粒类型:	哺乳细胞表达载体
高拷贝/低拷贝:	高拷贝
启动子:	EF-1α
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5818 bp
5' 测序引物及序列:	T7 Forward：TAATACGACTCACTATAGGG
3' 测序引物及序列:	--
载体标签:	His Tag (6x) , V5 Epitope Tag
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	Blasticidin
备注:	高表达水平。
产品目录号:	V961-20
稳定性:	瞬表达
组成型:	组成型表达
病毒/非病毒:	非病毒

pEF6/V5-His A质粒图谱 pEF6-V5-HisA载体图谱和pEF6/V5-His A载体序列 pEF6-V5-HisA质粒序列多克隆位点信息

pEF6-V5-His A载体图谱

pEF6/V5-His A质粒 pEF6-V5-HisA载体简介

pEF6/V5-His A, B, & C Mammalian Expression Vectors

All pEF or pUB vectors contain a strong promoter for high-level expression 
in mammalian cells, a choice of selection marker for generating stable 
cell lines, and an epitope tag for easy detection with a monoclonal antibody 
and rapid purification on nickel-chelating resin. Each vector is available 
in three reading frames to simplify cloning in-frame with the fusion tag.

pEF6/V5-His A质粒序列 pEF6-V5-HisA载体序列

AATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAA
TAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACGGATCGGGAGATCTC
CCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCC
TGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACC
GACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATA
CGCGTTGACATTGATTATTGACTAGGCTTTTGCAAAAAGCTTTGCAAAGATGGATAAAGTTTTAAACAGA
GAGGAATCTTTGCAGCTAATGGACCTTCTAGGTCTTGAAAGGAGTGGGAATTGGCTCCGGTGCCCGTCAG
TGGGCAGAGCGCACATCGCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCT
AGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGG
GGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACA
CAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAA
TTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTC
GAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGC
CGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATT
TTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACAC
TGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGG
CGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGC
CTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTG
AGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAG
CGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCA
CGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTG
GGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGG
CACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGA
CAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAGGAATTAGCTTGGTACTAATACGACTCAC
TATAGGGAGACCCAAGCTGGCTAGGTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAA
TTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCTTCGAAGGTAAGCCTATCCCTA
ACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAGTTTAAACCCGCTG
ATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACC
CTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGT
GTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCA
TGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCC
CACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTG
CCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCG
TCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAA
CTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGG
AGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTC
TTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTT
AACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAG
AAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGC
AGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGC
CCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGC
CGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGC
AAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAGCACGTGTTGACAATTAATCATCGGC
ATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGCCTTTGTCTCAAGAA
GAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCG
CCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACC
TTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCG
ATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATC
CTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCC
CTCTGGTTATGTGTGGGAGGGCTAAGCACTTCGTGGCCGAGGAGCAGGACTGACACGTGCTACGAGATTT
CGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATC
CTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTT
ACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTT
GTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCA
TGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCA
TAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGC
TTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTG
CGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAGCATGTG
AGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGC
CCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCT
GTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG
TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAG
GATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACT
AGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAA
AAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGT
TAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTT
TTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACC
TATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATA
CGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATT
TATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCAT
CCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTT
GCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAAC
GATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGT
TGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTC
ATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGC
GGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGT
GCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCG
ATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAA
AAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTT
CCTTTTTC

pEF6/V5-His A载体图谱 pEF6-V5-HisA质粒图谱pdf版和pEF6/V5-His A载体序列 pEF6-V5-HisA质粒序列等相关资料下载

pEF6/V5-His A质粒 pEF6-V5-HisA载体应用举例

上一篇：pEF6/V5-His TOPO TA
下一篇：pEBVHisA

pAAV-EFS-DIO-GRAB_OT1.0	pHT01
pENTR-L1pLaclacZaL4	FUW-tetO-hOCT4
pDNAPolbeta_TA	pHIL-D2
pAAVLINK-EF1a-HA-5-RALGAPB	pAAV-CAG-NES-YTnC
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG	pS-hydro_TA
pcDNA3.1/Zeo(-)	pFN10A (ACT)
pVK101	pIZT/V5-His
pCold-SUMO	pIEx/Bac-3
pCEP4-Flag-N	pBTB-5
pHY300PLK	pAAV-TRE-CAG-Fluc
pENTR U6	pPM408
pIEX-3	pMMB66EH
pYES2-EGFP	p1JI008
pWY113 pET28a_AtmtE2	pRI101-AN
pGL4.18[luc2P/Neo]	pAX01-ComK
pL-brevisGH1-TA	pQE-16
pcDNA3.1+N-DYK-P2A	pDB262
pShuttle-CMV	pCEP4-DsRed2
pYC25	pSuper.Retro.Neo
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG	pGEX-6p-1-hsG3BP2b
pQLinkN	pMAL-p5x
pAAVLINK-EF1a-HA-5'-ITSN1	pNov.aro-bgl1-640
pcDNA3.1-mRFP	pHisB-Sumo-NeMeHg
pAAVLINK-EF1a-HA-5'-DIP2C	YEp16
YEp620	pSW510
pBARMTE1	pLenti6/V5-GW/lacZ
pBABE-HAII	pUTK21
pFastBac1-His-C	pAAVLINKv1-pCALM1-Cre-3'-RAI1-FLAG
pSP65-F1+	pCDEF-HA-Ub-K63R
pET-53-DEST	pGEX-6p-1-AT5G48650
pIRESpuro3	pAAVLINKv1-pCALM1-Cre-3'-ITSN1-FLAG
pDM4	pGL4.21[luc2P/Puro]
pUC19	p19D+
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-POGZ-FLAG	pCRE-DD-AmCyan1
pBad33	pAAVLINK-EF1a-5'-PXDN
pAAV-hSyn-Cepheid1s-WPRE	pHR31
pcDNA3.1-CNTRFv1-HA-His	pIEX-1
pEYFP-Mem	pBR327
pMCS-Green-Renilla-Luc	pBBR1MCS2-Tac-mCherry
pAP1(PMA)-TA-Luc	pRetroX-Tight-Hyg
pHBT-GFP-NOS	pET28a-mOrange(M163A)
pFR-Luc	pSEAP2-Basic
ppyrG	pSwitch
pCRII-TOPO/22-1	pLVX-AmCyan1-N1
pcDNA6.2/nTC-Tag-DEST	pDEST8
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CADPS-FLAG	Pgex-HRV3C
pENTR5'/CMVp	pHOOK1
pAMβ1	YEp24
pBluescript II KS(-)	pGFP22
pLC4-14	pcDNA4/TO/Myc-His/LacZ

pKAR2-Br512	pscAAV-CAG-GFP
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g	pLenti-MP2
pRK5-HA-SH3BP4 W92A	Fuw-dCas9-Dnmt3a_IM
pMXs-hSOX2	SIRT6 Flag
pGHYB	phage UbiC G3BP1-SNAP
pCDNA3 Flag p38 alpha	pEF1a-Usp9x_CD-mCherry
EGFP-LC3	pHIV-Luciferase
pX330_SpCas9_USP7 Exon 3 gRNA	pLIC-SM-HA
pGP-CMV-Gcamp6m	pTA-HUH
pBiFC-VN155(I152L)	pCITEp65
MG1655 ΔendA ΔrecA (DE3)	pCS2+HyPer7-NLS
AdEasier-1 cells (strain)	AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-
pCMV-mA1-BE3	pLTR-RD114A
pET28GST-LIC	pCMV-HA-JMJD3
pTM1_VP30_ZEBOV	pcDNA3.1(+) CircRNA Mini Vector
pBSK	pCMT-flp
pRK5-HA-Ubiquitin-K29R	pcDNA3 Connexin43-GFP-APEX2
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA	pAKTaq
pBK092	pAAVS1-PC-GCaMP6f
pCAG-FAST-p10	pINDUCER21-puro_Gateway-3xFLAG
pSI-Target-AID-NG	pXR002:EF1a-dCasRx-2A-EGFP
mAzurite-8xbiRhoBAST-24xMS2v6	PA-GFPmt
BB3cK_pGAP_23*_pTEF_Cas9	CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGre
pCutamp	CAMK2D gRNA (BRDN0001146112)
pcDNA3.1-TYMS	pT7-SpCas9_sgRNA-site1 (RTW443)
pCRISPR-cBEST	pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
pMIH-TOMM20-Halo	LeGO-iG
pDawn-sfGFP	pCI-6.20
pcDNA3.1_AktPH-mCherry	BCR/ABL P190 transgenic construct
pET16B.Pfu	pKO (Hyg)
pNG9	shYAP1
pMR-A-3	murine TCR OTI-2A.pMIG II
pQCXIP-GFP-LacR	Double UP mNeon to mScarlet
pAc-sgRNA-Cas9	pGEX6p-1-BCCP-PupE
pEBTet_NAD-Snifit-mito	pCDH-DCX-TurboID-V5-puro
pcDNA3.1-ERBB4	pBLO 64.1
pCI-neo.mCherry-NEDD4	PB533_15F11-sfGFP
CD109-bio-His	pEGFP-C1 F-tractin-EGFP
pDriver	LentiGuide_DKO
GW1-Peredox-mCherry-NLS	MP6
pPB-AMBRA1-3xFLAG	pAAV2/9n
pQCasTns(Vch)-entry(BsaI)	pHCMV_DUGV_GP
GAG-CRErec	p6XHis-T7(P266L)
pcDNA3.1 SIRT6_H133Y	pT7-hMLH1dn for IVT
Myc tag WT PTB	pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3
pQUAST>stop>mCD8-GFP	pRH2502
PX551	pShuttle-CMV-GFP U6promoter
pBIG1a	pcDNA5-T/O-Ds1-mCherry
pJRH-1179 U6-reci Gag-Cas9 v2	pLminP_Luc2P_RE4
GFP-MAP1B	mARG1 cassette 3

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pEF6/V5-His A

买家导航

pEF6/V5-His A载体 pEF6-V5-HisA质粒基本信息

pEF6/V5-His A质粒图谱 pEF6-V5-HisA载体图谱和pEF6/V5-His A载体序列 pEF6-V5-HisA质粒序列多克隆位点信息

pEF6/V5-His A质粒 pEF6-V5-HisA载体简介

pEF6/V5-His A质粒序列 pEF6-V5-HisA载体序列

pEF6/V5-His A载体图谱 pEF6-V5-HisA质粒图谱pdf版和pEF6/V5-His A载体序列 pEF6-V5-HisA质粒序列等相关资料下载

pEF6/V5-His A质粒 pEF6-V5-HisA载体应用举例

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