pEBVHisA

LOCUS       Exported               10029 bp ds-DNA     circular SYN 13-AUG-2016
KEYWORDS    pEBVHisA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10029)
  AUTHORS   Biofeng Lab.
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10029
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    275..409
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     misc_feature    415..497
                     /note="MCS"
     CDS             complement(507..530)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /note="Xpress(TM) tag"
                     /translation="DLYDDDDK"
     CDS             complement(534..566)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /note="T7 tag (gene 10 leader)"
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(570..587)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     promoter        complement(603..864)
                     /note="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     polyA_signal    1630..1678
                     /note="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1720..2757)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     promoter        complement(2800..2945)
                     /note="HSV TK promoter"
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(3376..3964)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4135..4995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4996..5100)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 tctagagtcg acctcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga
       61 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      121 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagccgga tcataatcag
      181 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccggcctt
      421 gccggcctcg atcgagcggc cgctagcaag cttcgaattc ccatatggta ccagctgcag
      481 atctcgagct cggatcccca tcgatcctta tcgtcatcgt cgtacagatc ccgacccatt
      541 tgctgtccac cagtcatgct agccatacca tgatgatgat gatgatgaga accccccatg
      601 gaggtgcaca ccaatgtggt gaatggtcaa atggcgttta ttgtatcgag ctaggcactt
      661 aaatacaata tctctgcaat gcggaattca gtggttcgtc caatccatgt cagacccgtc
      721 tgttgccttc ctaataaggc acgatcgtac caccttactt ccaccaatcg gcatgcacgg
      781 tgctttttct ctccttgtaa ggcatgttgc taactcatcg ttaccatgtt gcaagactac
      841 aagagtattg cataagacta catttccccc tccctatgca aaagcgaaac tactatatcc
      901 tgaggggact cctaaccgcg tacaaccgaa gccccgcttt tcgcctaaac acaccctagt
      961 cccctcagat acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa cgcctaaccc
     1021 taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct taaattttgc
     1081 tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg cttaactatg
     1141 cggcatcaga gcagattgta ctgagagtgc accatacgga tctgcgatga taagctgtca
     1201 aacatgagaa ttggtcgact agcttggcac gccagaaatc cgcgcggtgg tttttggggg
     1261 tcgggggtgt ttggcagcca cagacgcccg gtgttcgtgt cgcgccagta catgcggtcc
     1321 atgcccaggc catccaaaaa ccatgggtct gtctgctcag tccagtcgtg gacctgaccc
     1381 cacgcaacgc ccaaaataat aacccccacg aaccataaac cattccccat gggggacccc
     1441 gtccctaacc cacggggcca gtggctatgg cagggcctgc cgccccgacg ttggctgcga
     1501 gccctgggcc ttcacccgaa cttggggggt ggggtgggga aaaggaagaa acgcgggcgt
     1561 attggcccca atggggtctc ggtggggtat cgacagagtg ccagccctgg gaccgaaccc
     1621 cgcgtttatg aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccgtc
     1681 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct cccccatctc
     1741 ccgatccgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
     1801 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
     1861 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
     1921 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagccgcgg
     1981 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
     2041 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
     2101 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
     2161 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
     2221 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
     2281 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
     2341 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
     2401 catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa
     2461 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga attccccaat
     2521 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
     2581 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
     2641 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
     2701 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
     2761 tcattgcccc ccgggatctg cggcacgctg ttgacgctgt taagcgggtc gctgcagggt
     2821 cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg aagtggacct cggaccgcgc
     2881 cgccccgact gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt
     2941 gtcatcatag aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc
     3001 gagcaacggg ccacggggat gaagcagggc ggcacctcgc taacggattc accactccaa
     3061 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
     3121 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg gggccgacgc
     3181 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     3241 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     3301 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3781 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     3841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     4141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4441 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4741 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     5041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     5101 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     5161 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat gtttgacagc
     5221 ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc gttgccccag
     5281 tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa tccccatccc
     5341 taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct caggacgcaa
     5401 atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat gaggacgagg
     5461 atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac catgaagggg
     5521 atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat tcactgagcg
     5581 tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga cgaggggcca
     5641 ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc agaaggctcc
     5701 ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg gggaagagga
     5761 cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg gccaagacat
     5821 agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa agggacccac
     5881 ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc aggagcagga
     5941 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg ggcaggagca
     6001 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg agcaggagga
     6061 ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc aggaggggca
     6121 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggagca
     6181 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg
     6241 gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg ggcaggaggg
     6301 gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga
     6361 gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc aggaggggca
     6421 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
     6481 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     6541 ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg agcaggaggt
     6601 ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg aggccggggt
     6661 cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg gggaagtcgt
     6721 gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag tcccagtagt
     6781 cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag gccatttttc
     6841 caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc agatggtgag
     6901 cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg agaaggccca
     6961 agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg gtggtttgga
     7021 aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga aggtttaaga
     7081 gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg ggtcgccggt
     7141 gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg aactgccctt
     7201 gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat ggcccctgga
     7261 cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat ggtcttttta
     7321 caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt tatgacaaag
     7381 cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg agtagatttg
     7441 cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga cggagatgac
     7501 ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg taacttgtta
     7561 ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga ataaatcccc
     7621 agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca ccattttcgt
     7681 cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc tccgcgctca
     7741 acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc agacatgcga
     7801 cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc aggaaaagga
     7861 caagcagcga aaattcacgc ccccttggga ggtggcggca tatgcaaagg atagcactcc
     7921 cactctacta ctgggtatca tatgctgact gtatatgcat gaggatagca tatgctaccc
     7981 ggatacagat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     8041 agatatagat taggatagcc tatgctaccc agatataaat taggatagca tatactaccc
     8101 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     8161 agatatagat taggatagca tatgctaccc agatatagat taggatagca tatgctatcc
     8221 agatatttgg gtagtatatg ctacccagat ataaattagg atagcatata ctacccagat
     8281 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
     8341 ataaattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
     8401 atagattagg atagcctatg ctacccagat atagattagg atagcatatg ctatccagat
     8461 atttgggtag tatatgctac ccatggcaac attagcccac cgtgctctca gcgacctcgt
     8521 gaatatgagg accaacaacc ctgtgcttgg cgctcaggcg caagtgtgtg taatttgtcc
     8581 tccagatcgc agcaatcgcg cccctatctt ggcccgccca cctacttatg caggtattcc
     8641 ccggggtgcc attagtggtt ttgtgggcaa gtggtttgac cgcagtggtt agcggggtta
     8701 caatcagcca agttattaca cccttatttt acagtccaaa accgcagggc ggcgtgtggg
     8761 ggctgacgcg tgcccccact ccacaatttc aaaaaaaaga gtggccactt gtctttgttt
     8821 atgggcccca ttggcgtgga gccccgttta attttcgggg gtgttagaga caaccagtgg
     8881 agtccgctgc tgtcggcgtc cactctcttt ccccttgtta caaatagagt gtaacaacat
     8941 ggttcacctg tcttggtccc tgcctgggac acatcttaat aaccccagta tcatattgca
     9001 ctaggattat gtgttgccca tagccataaa ttcgtgtgag atggacatcc agtctttacg
     9061 gcttgtcccc accccatgga tttctattgt taaagatatt cagaatgttt cattcctaca
     9121 ctagtattta ttgcccaagg ggtttgtgag ggttatattg gtgtcatagc acaatgccac
     9181 cactgaaccc cccgtccaaa ttttattctg ggggcgtcac ctgaaacctt gttttcgagc
     9241 acctcacata caccttactg ttcacaactc agcagttatt ctattagcta aacgaaggag
     9301 aatgaagaag caggcgaaga ttcaggagag ttcactgccc gctccttgat cttcagccac
     9361 tgcccttgtg actaaaatgg ttcactaccc tcgtggaatc ctgaccccat gtaaataaaa
     9421 ccgtgacagc tcatggggtg ggagatatcg ctgttcctta ggaccctttt actaacccta
     9481 attcgatagc atatgcttcc cgttgggtaa catatgctat tgaattaggg ttagtctgga
     9541 tagtatatac tactacccgg gaagcatatg ctacccgttt agggttaaca agggggcctt
     9601 ataaacacta ttgctaatgc cctcttgagg gtccgcttat cggtagctac acaggcccct
     9661 ctgattgacg ttggtgtagc ctcccgtagt cttcctgggc ccctgggagg tacatgtccc
     9721 ccagcattgg tgtaagagct tcagccaaga gttacacata aaggcaatgt tgtgttgcag
     9781 tccacagact gcaaagtctg ctccaggatg aaagccactc agtgttggca aatgtgcaca
     9841 tccatttata aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt
     9901 gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg
     9961 cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg
    10021 gatcgatcc
//