| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pEBVHisB |
| 质粒类型: | 哺乳动物表达载体；附加体载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | RSV |
| 载体大小: | 10019 bp |
| 5' 测序引物及序列: | pREP Forward 5’ GCTCGATACAATAAACGCC 3’ |
| 3' 测序引物及序列: | SV40pA rev 5'GAAATTTGTGATGCTATTGC' |
| 载体标签: | His tag (N-term) |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Hygromycin |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 人类细胞；灵长类细胞；犬类细胞 |
| 备注: |
pEBVHis-lacZ载体是附加体型载体，可以在基因组外自主复制，不需要整合和转座到基因组上，无需包装成病毒，即可实现稳定表达。其它附加体载体有： pREP4 PCEP4 pREP7 pREP10 pEBNA-DEST pEBVHisA pEBVHisB pEBVHisC pREP4/CAT pEBVHis-lacZ |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pEBVHisB载体 pEBVHisB质粒基本信息
pEBVHisB质粒图谱 pEBVHisB载体图谱和pEBVHisB载体序列 pEBVHisB质粒序列多克隆位点信息
pEBVHisB质粒 pEBVHisB载体简介
索取pEBVHis A,B,C载体说明书及蛋白表达与纯化的操作手册请E.Mail我们，或者在线留言。
pEBVHisB质粒序列 pEBVHisB载体序列
LOCUS pEBVHisB 10307 bp DNA SYN
DEFINITION pEBVHisB
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..10307
/organism="pEBVHisB"
/mol_type="other DNA"
misc_feature 112..134
/label="pGEX_3_primer"
terminator 279..410
/label="SV40_PA_terminator"
misc_feature 367..386
/label="EBV_rev_primer"
misc_feature complement(498..521)
/label="Xpress_EK"
misc_feature complement(525..557)
/label="T7_leader"
misc_feature complement(540..558)
/label="Xpress_fwd_primer"
misc_feature complement(624..642)
/label="pREP_fwd_primer"
CDS 1203..1940
/label="ORF frame 3"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
terminator complement(1461..1731)
/label="TK_PA_terminator"
/translation="MRIGRPLGARNPRGGFWGSGVFGSHRRPVFVSRQYMRSMPRPSK
NHGSVCSVQSWTRPHATPKIITPTNHKPFPMGDPVPNPRGQWLWQGLPPRRWLRALGL
HPNLGGGVGKRKKRGRIGPNGVSVGYRQSASPGTEPRVYEQTTQHPCVLFCLFIAVIA
RVPSGIVSFRVSVSLPHLPYSFALGRVLGRRFPLSASTSTQPSVQTAALLRAICVRPT
VPAPDRTIASHRPCAQAASSKLPSTKL*"
CDS complement(1739..2764)
/label="ORF frame 2"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
gene complement(1742..2749)
/label="hygroB"
/gene="hygroB"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKE*"
CDS 2006..3376
/label="ORF frame 2"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
promoter 2883..3031
/label="TATA_promoter"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
gene 3038..3376
/label="tet (852 - 1190)"
/gene="tet (852 - 1190)"
/translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
AGDAIGQALAEFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPGICGTLLTLLSGSLQGRSV
FEATRVTLICEVDLGPRRPDCICVFEFANDKTLGGVCVIIELKTCKYISSGDTASKRE
QRATGMKQGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDH
QGQLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAW
SRATST*"
CDS 3280..3891
/label="ORF frame 1"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
gene complement(4325..5185)
/label="Ampicillin"
/gene="Ampicillin"
/translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFT
TPRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRSKRPGTVKRPRCWRFSIGSAP
LTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDTCPPFSLREAWRFL
IAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*"
CDS complement(4325..5185)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5227..5255)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS complement(5811..7025)
/label="ORF frame 1"
/translation="MAFYLEGACAVETRMMMTDYWDSWASFLHVHDLSPWLFHDFPPW
LFHVLYPGGLHYLLDPGLHYLLDPGLHCLLDPGLHLLLLPLLLLPLLLLLPLLPLLLL
PLLPLLLLPLLPLLLLPLLPLLLLLPLLPLLLLLPLLPLLLLPLLPLLLLPLLPLLLL
PLLLLPLLLLPLLLLPLLPLLPLLLLLPLLLLPLLPLLPLLLLPLLLLLPLLPLLPLL
LLLPLLPLLLLLPLLLLLPLLPLLPLLLLLPLLPLLLLLPLLLLLPLLPLLPLLLLLP
LLLLLPLLPLLPLLPLLLLLPLLLLLPLLLLPLPLLLLLLFHRGSLCSQCNLDVFGVS
GHHLYVLALILSRPGLLVFRLLVLVLFPVLVHGYHPLFFEVHCRRSLLVQMCLPSLLG
HFQVLYLAPRQT*"
CDS 5813..7738
/label="ORF frame 2"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRG
RGRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGG
AGAGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAG
GGAGGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGA
GAGGGAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAG
GAGAGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGS
GGRGRGGSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSG
SPPRRPPPGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGP
RGQGDGGRRKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVF
VYGGSKTSLYNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFL
QTHIFAEVLKDAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDG
DDGDEGGDGDEGEEGQE*"
CDS 5928..7040
/label="ORF frame 3"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
rep_origin 8042..10212
/label="EBV_origin"
/translation="MDEDGEEDEDEEAEDQEPRAAQDQGQDIEMVSGDPKNVQVALAA
KGPTVEQEQEQEREGQEQEGQEQEEGQEQEEGQEGQEGQEGQEQEEGQEQEEGQEGQE
GQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQEEGQEQEEGQEGQEQEEGQEGQEGQEQ
EEGQEQEGQEGQEGQEQEGQEQEEGQEGQEGQEQEGQEQEGQEQEGQEQEGQEGQEQE
GQEGQEQEGQEGQEQEEGQEGQEQEEGQEGQEQEGQEGQEQEGQEGQEQEGQEGQEQE
EGQEQEGQEQEVEAGVEEAVEAGVEEVVEAGVEEVVEAAGVEDVKEPGGEVVKEPGGE
VVDVEKRGPGVPVVSHHHPGLHRAGPLQVEGHFSTL*"
ORIGIN
1 tctagagtcg accggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
61 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
121 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagccg gatcataatc
181 agccatacca catttgtaga ggttttactt gctttaaaaa acctccccac ctccccctga
241 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
301 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
361 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatccggcct
421 tgccggcctc gatcgagcgg ccgctagcaa gcttcgaatt cccatatggt accagctgca
481 gatctcgagc tcggatcctt atcgtcatcg tcgtacagat cccgacccat ttgctgtcca
541 ccagtcatgc tagccatacc atgatgatga tgatgatgag aaccccccat ggaggtgcac
601 accaatgtgg tgaatggtca aatggcgttt attgtatcga gctaggcact taaatacaat
661 tatctctgca atgcggaatt cagtggttcg tccaatccat gtcagacctg tctgttgcct
721 tcctaataag gcacgatcgt accaccttac ttccaccaat cggcatgcac ggtgcttttt
781 ctctccttgt aaggcatgtt gctaactcat cgttaccatg ttgcaagact acaagtgtat
841 tgcataagac tacatttccc cctccctatg caaaagcgaa actactatat cctgagggga
901 ctcctaaccg cgtacaaccg aagccccgct gggcgcctaa acacacccta gtcccctcag
961 atacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
1021 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
1081 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcagca
1141 gagcagattg tactgagagt gcaccataca agctcaggat ctgcgatgat aagctgtcaa
1201 acatgagaat tggtcgacca ctgggcgcca gaaatccgcg cggtggtttt tgggggtcgg
1261 gggtgtttgg cagccacaga cgcccggtgt tcgtgtcgcg ccagtacatg cggtccatgc
1321 ccaggccatc caaaaaccat gggtctgtct gctcagtcca gtcgtggacc agaccccacg
1381 caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
1441 ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
1501 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
1561 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
1621 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
1681 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccct
1741 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
1801 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
1861 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
1921 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
1981 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
2041 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
2101 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
2161 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
2221 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
2281 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
2341 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
2401 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
2461 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
2521 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
2581 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
2641 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
2701 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
2761 tcatatctca ttgcccggga tctgcggcac gctgttgacg ctgttaagcg ggtcgctgca
2821 gggtcgctcg gtgttcgagg ccacacgcgt caccttaata tgcgaagtgg acctgggacc
2881 gcgccgcccc gactgcatct gcgtgttcga attcgccaat gacaagacgc tgggcggggt
2941 ttgtgtcatc atagaactaa agacatgcaa atatatttct tccggggaca ccgccagcaa
3001 acgcgagcaa cgggccacgg ggatgaagca gggcatggcg gccgacgcgc tgggctacgt
3061 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
3121 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
3181 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
3241 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
3301 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
3361 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
3421 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
3481 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcagcaaaag gccaggaacc
3541 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
3601 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
3661 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
3721 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
3781 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
3841 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
3901 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
3961 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
4021 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
4081 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
4141 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
4201 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
4261 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
4321 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
4381 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
4441 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
4501 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
4561 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
4621 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
4681 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
4741 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
4801 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
4861 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
4921 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
4981 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
5041 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
5101 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
5161 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
5221 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
5281 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
5341 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattctcat
5401 gtttgacagc ttatcatcgc gataagctga tcctcacagg ccgcacccag cttttcttcc
5461 gttgccccag tagcatctct gtctggtgac cttgaagagg aagaggaggg gtcccgagaa
5521 tccccatccc taccgtccag caaaaagggg gacgaggaat ttgaggcctg gcttgaggct
5581 caggacgcaa atcttgagga tgttcagcgg gagttttccg ggctgcgagt aattggtgat
5641 gaggacgagg atggttcgga ggatggggaa ttttcagacc tggatctgtc tgacagcgac
5701 catgaagggg atgagggtgg gggggctgtt ggagggggca ggagtctgca ctccctgtat
5761 tcactgagcg tcgtctaata aagatgtcta ttgatctctt ttagtgtgaa tcatgtctga
5821 cgaggggcca ggtacaggac ctggaaatgg cctaggagag aagggagaca catctggacc
5881 agaaggctcc ggcggcagtg gacctcaaag aagagggggt gataaccatg gacgaggacg
5941 gggaagagga cgaggacgag gaggcggaag accaggagcc ccgggcggct caggatcagg
6001 gccaagacat agagatggtg tccggagacc ccaaaaacgt ccaagttgca ttggctgcaa
6061 agggacccac ggtggaacag gagcaggagc aggagcggga ggggcaggag caggaggggc
6121 aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagg
6181 ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg
6241 agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag gaggaggggc
6301 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
6361 ggcaggagca ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg
6421 agcaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggagg
6481 ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggagggg caggagcagg
6541 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggcaggagc
6601 aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg
6661 ggcaggaggg gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc
6721 aggagcagga ggggcaggag gggcaggagc aggaggaggg gcaggagcag gaggggcagg
6781 agcaggaggt ggaggccggg gtcgaggagg cagtggaggc cggggtcgag gaggtagtgg
6841 aggccggggt cgaggaggta gtggaggccg ccggggtaga ggacgtgaaa gagccagggg
6901 gggaagtcgt gaaagagcca gggggagagg tcgtggacgt ggagaaaaga ggcccaggag
6961 tcccagtagt cagtcatcat catccgggtc tccaccgcgc aggccccctc caggtagaag
7021 gccatttttc caccctgtag gggaagccga ttattttgaa taccaccaag aaggtggccc
7081 agatggtgag cctgacgtgc ccccgggagc gatagagcag ggccccgcag atgacccagg
7141 agaaggccca agcactggac cccggggtca gggtgatgga ggcaggcgca aaaaaggagg
7201 gtggtttgga aagcatcgtg gtcaaggagg ttccaacccg aaatttgaga acattgcaga
7261 aggtttaaga gctctcctgg ctaggagtca cgtagaaagg actaccgacg aaggaacttg
7321 ggtcgccggt gtgttcgtat atggaggtag taagacctcc ctttacaacc taaggcgagg
7381 aactgccctt gctattccac aatgtcgtct tacaccattg agtcgtctcc cctttggaat
7441 ggcccctgga cccggcccac aacctggccc gctaagggag tccattgtct gttatttcat
7501 ggtcttttta caaactcata tatttgctga ggttttgaag gatgcgatta aggaccttgt
7561 tatgacaaag cccgctccta cctgcaatat cagggtgact gtgtgcagct ttgacgatgg
7621 agtagatttg cctccctggt ttccacctat ggtggaaggg gctgccgcgg agggtgatga
7681 cggagatgac ggagatgaag gaggtgatgg agatgagggt gaggaagggc aggagtgatg
7741 taacttgtta ggagacgccc tcaatcgtat taaaagccgt gtattccccc gcactaaaga
7801 ataaatcccc agtagacatc atgcgtgctg ttggtgtatt tctggccatc tgtcttgtca
7861 ccattttcgt cctcccaaca tggggcaatt gggcataccc atgttgtcac gtcactcagc
7921 tccgcgctca acaccttctc gcgttggaaa acattagcga catttacctg gtgagcaatc
7981 agacatgcga cggctttagc ctggcctcct taaattcacc taagaatggg agcaaccagc
8041 atgcaggaaa aggacaagca gcgaaaattc acgccccctt gggaggtggc ggcatatgca
8101 aaggatagca ctcccactct actactgggt atcatatgct gactgtatat gcatgaggat
8161 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
8221 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
8281 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
8341 agcctatgct acccagatat agattaggat agcatatgct acccagatat agattaggat
8401 agcatatgct atccagatat ttgggtagta tatgctaccc agatataaat taggatagca
8461 tatactaccc taatctctat taggatagca tatgctaccc ggatacagat taggatagca
8521 tatactaccc agatatagat taggatagca tatgctaccc agatatagat taggatagcc
8581 tatgctaccc agatataaat taggatagca tatactaccc agatatagat taggatagca
8641 tatgctaccc agatatagat taggatagcc tatgctaccc agatatagat taggatagca
8701 tatgctatcc agatatttgg gtagtatatg ctacccatgg caacattagc ccaccgtgct
8761 ctcagcgacc tcgtgaatat gaggaccaac aaccctgtgc ttggcgctca ggcgcaagtg
8821 tgtgtaattt gtcctccaga tcgcagcaat cgcgccccta tcttggcccg cccacctact
8881 tatgcaggta ttccccgggg tgccattagt ggttttgtgg gcaagtggtt tgaccgcagt
8941 ggttagcggg gttacaatca gccaagttat tacaccctta ttttacagtc caaaaccgca
9001 gggcggcgtg tgggggctga cgcgtgcccc cactccacaa tttcaaaaaa aagagtggcc
9061 acttgtcttt gtttatgggc cccattggcg tggagccccg tttaattttc gggggtgtta
9121 gagacaacca gtggagtccg ctgctgtcgg cgtccactct ctttcccctt gttacaaata
9181 gagtgtaaca acatggttca cctgtcttgg tccctgcctg ggacacatct taataacccc
9241 agtatcatat tgcactagga ttatgtgttg cccatagcca taaattcgtg tgagatggac
9301 atccagtctt tacggcttgt ccccacccca tggatttcta ttgttaaaga tattcagaat
9361 gtttcattcc tacactagta tttattgccc aaggggtttg tgagggttat attggtgtca
9421 tagcacaatg ccaccactga accccccgtc caaattttat tctgggggcg tcacctgaaa
9481 ccttgttttc gagcacctca catacacctt actgttcaca actcagcagt tattctatta
9541 gctaaacgaa ggagaatgaa gaagcaggcg aagattcagg agagttcact gcccgctcct
9601 tgatcttcag ccactgccct tgtgactaaa atggttcact accctcgtgg aatcctgacc
9661 ccatgtaaat aaaaccgtga cagctcatgg ggtgggagat atcgctgttc cttaggaccc
9721 ttttactaac cctaattcga tagcatatgc ttcccgttgg gtaacatatg ctattgaatt
9781 agggttagtc tggatagtat atactactac ccgggaagca tatgctaccc gtttagggtt
9841 aacaaggggg ccttataaac actattgcta atgccctctt gagggtccgc ttatcggtag
9901 ctacacaggc ccctctgatt gacgttggtg tagcctcccg tagtcttcct gggcccctgg
9961 gaggtacatg tcccccagca ttggtgtaag agcttcagcc aagagttaca cataaaggca
10021 atgttgtgtt gcagtccaca gactgcaaag tctgctccag gatgaaagcc actcagtgtt
10081 ggcaaatgtg cacatccatt tataaggatg tcaactacag tcagagaacc cctttgtgtt
10141 tggtcccccc ccgtgtcaca tgtggaacag ggcccagttg gcaagttgta ccaaccaact
10201 gaagggatta catgcactgc cccgaataca aaacaaaagc gctcctcgta ccagcgaaga
10261 aggggcagag atgccgtagt caggtttagt tcgtccggcg gcggggc
//
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