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P416GPD载体p416 GPD质粒基本信息
P416GPD质粒图谱p416 GPD载体图谱和P416GPD载体序列p416 GPD质粒序列多克隆位点信息
P416GPD质粒p416 GPD载体简介
P416GPD质粒序列p416 GPD载体序列
LOCUS P416GPD 5778 bp DNA circular SYN 10-JUL-2023 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5778) AUTHORS Biofeng TITLE Direct Submission JOURNAL Exported Jul 10, 2023 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..5778 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 70..573 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 610..714 /gene="bla" /label=AmpR promoter CDS 715..1575 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1746..2334 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2658..2688 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2696..2712 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2720..2736 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2757..2775 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter 2800..3443 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase; also known as the TDH3 promoter" primer_bind 3449..3465 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3499..3515) /label=KS primer /note="common sequencing primer, one of multiple similar variants" terminator 3518..3765 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" promoter complement(3784..3802) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3812..3828) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" rep_origin 3973..4428 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" CDS complement(4559..5362) /codon_start=1 /gene="S. cerevisiae URA3" /product="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /label=URA3 /note="yeast auxotrophic marker, counterselectable with 5-fluoroorotic acid (5-FOA)" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" promoter complement(5363..5578) /gene="S. cerevisiae URA3" /label=URA3 promoter ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt 2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat 2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa 2941 tatataacat cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg 3001 gagcccgctt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt 3061 ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg 3121 cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa 3181 tgatgacaca aggcaattga cccacgcatg tatctatctc attttcttac accttctatt 3241 accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga 3301 aattattccc ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg 3361 taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt 3421 aaaacaccag aacttagttt cgacggattc tagaactagt ggatcccccg ggctgcagga 3481 attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc 3541 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct 3601 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata 3661 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa 3721 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt 3781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact 3841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 3901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 3961 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 4021 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 4081 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 4141 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 4201 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 4261 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 4321 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 4381 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc tgatgcggta 4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt 4501 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt 4561 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac 4621 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag 4681 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt 4741 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca 4801 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag 4861 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca 4921 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac 4981 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac 5041 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga 5101 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa 5161 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa 5221 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct 5281 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt 5341 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca 5401 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata 5461 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa 5521 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt 5581 gaaaagctgt ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc 5641 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat 5701 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 5761 catcaccgaa acgcgcga //