| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pMET B, pMETB |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7779 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 利用甲醇诱导蛋白在methanoloica酵母中表达。 |
| 产品目录号: | K1780-01 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMET B载体 pMETB质粒基本信息
pMET B质粒图谱 pMETB载体图谱和pMET B载体序列 pMETB质粒序列多克隆位点信息
pMET B质粒 pMETB载体简介
pMET B质粒序列 pMETB载体序列
LOCUS pMET B 7779 bp DNA SYN
DEFINITION pMET B
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..7779
/organism="pMET B"
/mol_type="other DNA"
misc_feature 1106..1130
/label="AUG1_fwd_primer"
misc_feature complement(1398..1419)
/label="AUG1_rev_primer"
CDS 2821..3396
/label="ORF frame 1"
CDS 3402..4535
/label="ORF frame 3"
CDS 4699..5697
/label="ORF frame 1"
gene complement(6510..7370)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(6510..7370)
/label="ORF frame 2"
promoter complement(7412..7440)
/label="AmpR_promoter"
ORIGIN
1 GGCGCGCCTT AATTAAGGCC GGCCCCTGCA GGGGGCCCCG AGATGGTACA TACTTAAAAG
61 CTGCCATATT GAGGAACTTC AAAGTTTTAT CTGTTTTTAG AATTAAAAGA CGATTGTTGT
121 AACAAAACGT TGTGCCTACA TAAACTCAAA TTAATGGAAA TAGCCTGTTT TGAAAAATAC
181 ACCTTCTTAA GTACTGACAA AGTTTTGTTA AATGACTATC GAACAAGCCA TGAAATAGCA
241 CATTTCTGCC AGTCACTTTT AACACTTTCC TGCTTGCTGG TTGACTCTCC TCATACAAAC
301 ACCCAAAAGG GAAACTTTCA GTGTGGGGAC ACTTGACATC TCACATGCAC CCCAGATTAA
361 TTTCCCCAGA CGATGCGGAG ACAAGACAAA ACAACCCTTT GTCCTGCTCT TTTCTTTCTC
421 ACACCGCGTG GGTGTGTGCG CAGGCAGGCA GGCAGGCAGC GGGCTGCCTG CCATCTCTAA
481 TCGCTGCTCC TCCCCCCTGG CTTCAAATAA CAGCCTGCTG CTATCTGTGA CCAGATTGGG
541 ACACCCCCCT CCCCTCCGAA TGATCCATCA CCTTTTGTCG TACTCCGACA ATGATCCTTC
601 CCTGTCATCT TCTGGCAATC AGCTCCTTCA ATAATTAAAT CAAATAAGCA TAAATAGTAA
661 AATCGCATAC AAACGTCATG AAAAGTTTTA TCTCTATGGC CAACGGATAG TCTATCTGCT
721 TAATTCCATC CACTTTGGGA ACCGTTCTCT CTTTACCCCA GATTCTCAAA GCTAATATCT
781 GCCCCTTGTC TATTGTCCTT TCTCCGTGTA CAAGCGGAGC TTTTGCCTCC CATCCTCTTG
841 CTTTGTTTCG GTTATTTTTT TTTTCTTTTG AAACTCTTGG TCAAATCAAA TCAAACAAAA
901 CCAAACCTTC TATTCCATCA GATCAACCTT GTTCAACATT CTATAAATCG ATATAAATAT
961 AACCTTATCC CTCCCTTGTT TTTTACCAAT TAATCAATCT TCAAATTTCA AATATTTTCT
1021 ACTTGCTTTA TTACTCAGTA TTAACATTTG TTTAAACCAA CTATAACTTT TAACTGGCTT
1081 TAGAAGTTTT ATTTAACATC AGTTTCAATT TACATCTTTA TTTATTAACG AAATCTTTAC
1141 GAATTAACTC AATCAAAACT TTTACGAAAA AAAAATCTTA CTATTAAGAG CTCGAGAGGA
1201 ATTCACGTGG CCCAGCCGGC CGTGGATCCA CGCGTCGTCG ACCCGCGGCG GCCGCCAGCT
1261 TACTAGTAGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG CGTACCGGTC
1321 ATCATCACCA TCACCATTGA TCTAGTATAC AATTCTAGGG CTGCCTGTTT GGATATTTTT
1381 ATAATTTTTG AGAGTTTGCC AACTAATGTT TTTCTCTTCT ATGATATTTA TCATGTAGTT
1441 GGATATCTTT TTTTCTTTGA AAAGTTTTTA TTTAGTTCTT TCTCTCTTTT TTATAATGTT
1501 TTCAGTTTCT TTCCTTTATA TTGAAAATAC GATGTTCTTT GTCTTTCTTT TTTAAATCCT
1561 CAATTATAAT GTGTACCGTT TGATCAACTT GTAAAATTGT TTGTAGTCCT GTAATCTTAA
1621 TTGACGATTG GTTGATAGTT TACCCCAGTA TATTGTGCGG CTGAGATATT ACCCGATTCG
1681 GAAGATTGAT TTTTTCAACA GACATAAACC CATAGGAGAT TTAGTACAGC GACATATGCT
1741 AATTGCCTGA TAATGCTCAC TAACATTTTC TCCTTTTAAT CTGGTATATG CATACAGGTA
1801 TGTTGATATG GCCTTGTTGA TCAAAGCTAC GAAAGTGTAG ACTATTTCCC AAACAGCCCC
1861 CAACCACCAC CTCACACGCG CACACTGCGA AGTGAAAGCG CTCCCTTATT TTCTTCTCCG
1921 CTGGTTCAAA ACAAAAAAGA ATCTAATCTG TAGACACACG CCCCGTAAAA CCCAATCTTC
1981 ATCATCGTAA TTTTCAGTGA CGGACGATCG ATCAACCATA TAGTTTGTTA GTGACTGAGC
2041 TTCAGTAGTT GACCTCTTGA AGAAATAACG TCTTGAACAA CATACTTTAT ACCTACCAAC
2101 ATTCGCCATC AACAACAGTG TGACACTGAC ATAACTTTAC ATCCATTCAT CGACAAAAAA
2161 ATGTCTCTAG TTCGGATTCA TGCTTATGGG TTAGCTATTT CGCCCAATGT GTCCATCTGA
2221 CATTACTATT TTGCATTTTA ATTTAATTAG AACTTGACTA GCGCACTACC AGCATATCAT
2281 CTCATTTCCG TAAATACCAA ATGTATTATA TATTGAAAGC TTTTGACCAG GTTATTATAA
2341 AAGAAACTTC ATGCTCGAAA AAGATCATTT CGAAAAGTTG CCTAGTTTCA TGAAATTTTA
2401 AAGCAGTTTA TATAAATTTT ACCTTTTGAT GCGGAATTGA CTTTTTCTTG AATAATACAT
2461 AACTTTTCTT AAAAGAATCA AAGACAGATA AAATTTAAGA GATATTAAAT ATTAGTGAGA
2521 AGCCGAGAAT TTTGTAACAC CAACATAACA CTGACATCTT TAACAACTTT TAATTATGAT
2581 ACATTTCTTA CGTCATGATT GATTATTACA GCTATGCTGA CAAATGACTC TTGTTGCATG
2641 GCTACGAACC GGGTAATACT AAGTGATTGA CTCTTGCTGA CCTTTTATTA AGAACTAAAT
2701 GGACAATATT ATGGAGCATT TCATGTATAA ATTGGTGCGT AAAATCGTTG GATCTCTCTT
2761 CTAAGTACAT CCTACTATAA CAATCAAGAA AAACAAGAAA ATCGGACAAA ACAATCAAGT
2821 ATGGATTCTA GAACAGTTGG TATATTAGGA GGGGGACAAT TGGGACGTAT GATTGTTGAG
2881 GCAGCAAACA GGCTCAACAT TAAGACGGTA ATACTAGATG CTGAAAATTC TCCTGCCAAA
2941 CAAATAAGCA ACTCCAATGA CCACGTTAAT GGCTCCTTTT CCAATCCTCT TGATATCGAA
3001 AAACTAGCTG AAAAATGTGA TGTGCTAACG ATTGAGATTG AGCATGTTGA TGTTCCTACA
3061 CTAAAGAATC TTCAAGTAAA ACATCCCAAA TTAAAAATTT ACCCTTCTCC AGAAACAATC
3121 AGATTGATAC AAGACAAATA TATTCAAAAA GAGCATTTAA TCAAAAATGG TATAGCAGTT
3181 ACCCAAAGTG TTCCTGTGGA ACAAGCCAGT GAGACGTCCC TATTGAATGT TGGAAGAGAT
3241 TTGGGTTTTC CATTCGTCTT GAAGTCGAGG ACTTTGGCAT ACGATGGAAG AGGTAACTTC
3301 GTTGTAAAGA ATAAGGAAAT GATTCCGGAA GCTTTGGAAG TACTGAAGGA TCGTCCTTTG
3361 TCGCCGAAAA ATGGGCACCA TTTACTAAAG AATTAGCAGT CATGATTGTG AGATCTGTTA
3421 ACGGTTTAGT GTTTTCTTAC CCAATTGTAG AGACTATCCA CAAGGACAAT ATTTGTGACT
3481 TATGTTATGC GCCTGCTAGA GTTCCGGACT CCGTTCAACT TAAGGCGAAG TTGTTGGCAG
3541 AAAATGCAAT CAAATCTTTT CCCGGTTGTG GTATATTTGG TGTGGAAATG TTCTATTTAG
3601 AAACAGGGGA ATTGCTTATT AACGAAATTG CCCCAAGGCC TCACAACTCT GGACATTATA
3661 CCATTGATGC TTGCGTCACT TCTCAATTTG AAGCTCATTT GAGATCAATA TTGGATTTGC
3721 CAATGCCAAA GAATTTCACA TCTTTCTCCA CCATTACAAC GAACGCCATT ATGCTAAATG
3781 TTCTTGGAGA CAAACATACA AAAGATAAAG AGCTAGAAAC TTGCGAAAGA GCATTGGCGA
3841 CTCCAGGTTC CTCAGTGTAC TTATATGGAA AAGAGTCTAG ACCTAACAGA AAAGTAGGTC
3901 ACATAAATAT TATTGCCTCC AGTATGGCGG AATGTGAACA AAGGCTGAAC TACATTACAG
3961 GTAGAACTGA TATTCCAATC AAAATCTCTG TCGCTCAAAA GTTGGACTTG GAAGCAATGG
4021 TCAAACCATT GGTTGGAATC ATCATGGGAT CAGACTCTGA CTTGCCGGTA ATGTCTGCCG
4081 CATGTGCGGT TTTAAAAGAT TTTGGCGTTC CATTTGAAGT GACAATAGTC TCTGCTCATA
4141 GAACTCCACA TAGGATGTCA GCATATGCTA TTTCCGCAAG CAAGCGTGGA ATTAAAACAA
4201 TTATCGCTGG AGCTGGTGGG GCTGCTCACT TGCCAGGTAT GGTGGCTGCA ATGACACCAC
4261 TTCCTGTCAT CGGTGTGCCC GTAAAAGGTT CTTGTCTAGA TGGAGTAGAT TCTTTACATT
4321 CAATTGTGCA AATGCCTAGA GGTGTTCCAG TAGCTACCGT CGCTATTAAT AATAGTACGA
4381 ACGCTGCGCT GTTGGCTGTC AGACTGCTTG GCGCTTATGA TTCAAGTTAT ACAACGAAAA
4441 TGGAACAGTT TTTATTAAAG CAAGAAGAAG AAGTTCTTGT CAAAGCACAA AAGTTAGAAA
4501 CTGTCGGTTA CGAAGCTTAT CTAGAAAACA AGTAATATAT AAGTTTATTG ATATACTTGT
4561 ACAGCAAATA ATTATAAAAT GATATACCTA TTTTTTAGGC TTTGTTATGA TTACATCAAA
4621 TGTGGACTTC ATACATAGAA ATCAACGCTT ACAGGTGTCC TTTTTTAAGA ATTTCATACA
4681 TAAGATCTAC TAGAATCAAT GTTAAACCTG TTATCATTAG AACAGCCATC TCCAGAAGGT
4741 TCTATCCAAG TGAAAACTTT GGTCTTCAAG CCAAAGACTG CTAAAACACA AACCCCAGTC
4801 CCAATTATTG TTGTTGCTGA AACTTCTACT TCTACTCCGG GTGGTGTTAT AGCCAAAGCT
4861 TCGGGCACTA AAGAGCCAAG ATTAGCAAAC GATGAATTGA CCAACCAATT CTTTGGTTCT
4921 CCTGCTAAAG AGTTTTCTAT TGCCAGCTTA TCAAACGAAC ACAAAGACGG TAAAGTCAAG
4981 ATCATTATTG ATGCTGGGAT CAAAGATGAT GACTCGACTA ATTACGTTAT TTCCAACACT
5041 GTTGCTAAGT TAGTTTTAAC CGGTAAGCAA TTATTAGGTT TCTTATCCAA CTACGAACCA
5101 ACCATCGTTG ATTTTACTGC TGAAGCTGCA ACCGCTGCCC CAACTCCAGT TGTTAAGAAA
5161 GAACCTGCTG CATCTGGTAA GCTTGATGAT GCCAAATTAA TTGGTGTTAC TGCTGACAAG
5221 GAAAAAGATT TCTCCAACTG GTACTCCCAA ATTCTAATGA AGGGTGAAAT GTTAGACTAC
5281 TACGATGTCT CTGGTTGTTA CATTATGAGA CCAGCTTCTT ACACAATTTG GGAAAAGATT
5341 CAATCTTGGT TCGATTCGAA GATTAAAGAA GATGGTGTTA CTAATGCTTA TTTCCCAATG
5401 TTTGTCTCAT CCAAGGTTTT AGAAAAGGAA AAGGACCACA TCGATGGTTT CGCTCCTGAA
5461 GTTGCTTGGG TTACCAGAGC TGGTAAATCT GAATTAGAAG AACCAATTGC TATTAGACCA
5521 ACTTCTGAAA CTGTTATGGG TACCCTGCAG GGGCCGGCCT TAATTAAGGC GCGCCCTTCC
5581 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA
5641 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA
5701 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG
5761 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
5821 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT
5881 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT
5941 TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC
6001 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
6061 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
6121 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC
6181 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA
6241 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT
6301 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT
6361 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
6421 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA
6481 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC
6541 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT
6601 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC
6661 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
6721 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA
6781 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG
6841 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT
6901 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC
6961 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT
7021 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC
7081 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC
7141 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA
7201 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC
7261 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA
7321 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT
7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
7501 GTATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC AGGAAATTGT
7561 AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA
7621 CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT
7681 GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA
7741 AGGGCGAAAA ACCGTCTATC AAGGGCGATG GCCCACTAC
//
