| 质粒名称: | BC-GPR56 |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 44197 |
| 存储实验室: | Lei Xu |
|
载体骨架基本信息 |
|
| 载体名称: | Barcode |
| 载体出品公司: | 未知 |
| 空载体大小: | 7500bp |
| 完整质粒大小: | 9435 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达、Retroviral |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | human GPR56 / GPR56 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2500 bp |
| 基因突变: | none |
| GenBank编号 | NM_001145770.1 NM_001145771.1 |
| 启动子: | LTR |
| 标签/融合蛋白: | none |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | n/a |
| 3’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | BC-GPR56 was a gift from Lei Xu (Addgene plasmid # 44197 ; http://n2t.net/addgene:44197 ; RRID:Addgene_44197) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS BC-GPR56 9435 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS BC-GPR56
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9435)
TITLE BC-GPR56
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9435)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9435
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 580..921
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 988..1404
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1334..1356
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1372..1388
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 3214..3223
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind 3656..3673
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3657..3673
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3680..3699
/label=T7
/note="T7 promoter, forward primer"
promoter 3680..3698
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 3749..4210
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3805..3822)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4032..4051
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4251..4847
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4251..4270)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4747..4767
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 5748..6263
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(6432..6448)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6432..6448)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6445..6467)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6456..6472
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6480..6510)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6525..6546
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6663..6680)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6834..7422)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6914..6933)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7593..8453)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8216..8235
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8454..8558)
/gene="bla"
/label=AmpR promoter
primer_bind 8626..8644
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8682..8704)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 8804..8823
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 9215..9234
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 9434..516
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
361 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat
421 aaagcctctt gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc
481 agattgattg actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag
541 acccctgcct agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc
601 gtgtctgtct ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg
661 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
721 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
781 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
841 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
901 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
961 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
1021 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
1081 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
1141 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
1201 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
1261 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
1321 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
1381 gccctcactc cttctctagg cgccggaaat tagatctacc ggtaagagtg actccgtcgg
1441 aggaaaatga ctccccagtc gctgctgcag acgacactgt tcctgctgag tctgctcttc
1501 ctggtccaag gtgcccacgg caggggccac agggaagact ttcgcttctg cagccagcgg
1561 aaccagacac acaggagcag cctccactac aaacccacac cagacctgcg catctccatc
1621 gagaactccg aagaggccct cacagtccat gcccctttcc ctgcagccca ccctgcttcc
1681 cgatccttcc ctgaccccag gggcctctac cacttctgcc tctactggaa ccgacatgct
1741 gggagattac atcttctcta tggcaagcgt gacttcttgc tgagtgacaa agcctctagc
1801 ctcctctgct tccagcacca ggaggagagc ctggctcagg gccccccgct gttagccact
1861 tctgtcacct cctggtggag ccctcagaac atcagcctgc ccagtgccgc cagcttcacc
1921 ttctccttcc acagtcctcc ccacacggcc gctcacaatg cctcggtgga catgtgcgag
1981 ctcaaaaggg acctccagct gctcagccag ttcctgaagc atccccagaa ggcctcaagg
2041 aggccctcgg ctgcccccgc cagccagcag ttgcagagcc tggagtcgaa actgacctct
2101 gtgagattca tgggggacat ggtgtccttc gaggaggacc ggatcaacgc cacggtgtgg
2161 aagctccagc ccacagccgg cctccaggac ctgcacatcc actcccggca ggaggaggag
2221 cagagcgaga tcatggagta ctcggtgctg ctgcctcgaa cactcttcca gaggacgaaa
2281 ggccggagcg gggaggctga gaagagactc ctcctggtgg acttcagcag ccaagccctg
2341 ttccaggaca agaattccag ccacgtcctg ggtgagaagg tcttggggat tgtggtacag
2401 aacaccaaag tagccaacct cacggagccc gtggtgctca ccttccagca ccagctacag
2461 ccgaagaatg tgactctgca atgtgtgttc tgggttgaag accccacatt gagcagcccg
2521 gggcattgga gcagtgctgg gtgtgagacc gtcaggagag aaacccaaac atcctgcttc
2581 tgcaaccact tgacctactt tgcagtgctg atggtctcct cggtggaggt ggacgccgtg
2641 cacaagcact acctgagcct cctctcctac gtgggctgtg tcgtctctgc cctggcctgc
2701 cttgtcacca ttgccgccta cctctgctcc agggtgcccc tgccgtgcag gaggaaacct
2761 cgggactaca ccatcaaggt gcacatgaac ctgctgctgg ccgtcttcct gctggacacg
2821 agcttcctgc tcagcgagcc ggtggccctg acaggctctg aggctggctg ccgagccagt
2881 gccatcttcc tgcacttctc cctgctcacc tgcctttcct ggatgggcct cgaggggtac
2941 aacctctacc gactcgtggt ggaggtcttt ggcacctatg tccctggcta cctactcaag
3001 ctgagcgcca tgggctgggg cttccccatc tttctggtga cgctggtggc cctggtggat
3061 gtggacaact atggccccat catcttggct gtgcatagga ctccagaggg cgtcatctac
3121 ccttccatgt gctggatccg ggactccctg gtcagctaca tcaccaacct gggcctcttc
3181 agcctggtgt ttctgttcaa catggccatg ctagccacca tggtggtgca gatcctgcgg
3241 ctgcgccccc acacccaaaa gtggtcacat gtgctgacac tgctgggcct cagcctggtc
3301 cttggcctgc cctgggcctt gatcttcttc tcctttgctt ctggcacctt ccagcttgtc
3361 gtcctctacc ttttcagcat catcacctcc ttccaaggct tcctcatctt catctggtac
3421 tggtccatgc ggctgcaggc ccggggtggc ccctcccctc tgaagagcaa ctcagacagc
3481 gccaggctcc ccatcagctc gggcagcacc tcgtccagcc gcatctagaa gggcgaattc
3541 tgcagatatc catcacactg gcggccgcag cacagtggtc gaaattctgg cgaaaggggg
3601 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
3661 aacgacggcc agtgaattgt aatacgactc actatagggc gaattaattc cggttatttt
3721 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
3781 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
3841 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
3901 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
3961 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
4021 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
4081 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
4141 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
4201 acgatgataa tatggccacc acccatatga aagaaaccgc tgctgctaaa atgaccgagt
4261 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
4321 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
4381 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
4441 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
4501 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
4561 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
4621 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
4681 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
4741 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
4801 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctgg tcgacaagca
4861 tggggtaaag tactgttctc atcacatcat atcaaggtta tataccatca atattgccac
4921 agatgttact tagcctttta atatttctct aatttagtgt atatgcaatg atagttctct
4981 gatttctgag attgagtttc tcatgtgtaa tgattattta gagtttctct ttcatctgtt
5041 caaatttttg tctagtttta ttttttactg atttgtaaga cttcttttta taatctgcat
5101 attacaattc tctttactgg ggtgttgcaa atattttctg tcattctatg gcctgacttt
5161 tcttaatggt tttttaattt taaaaataag tcttaatatt catgcaatct aattaacaat
5221 cttttctttg tggttaggac tttgagtcat aagaaatttt tctctacact gaagtcatga
5281 tggcatgctt ctatattatt ttctaaaaga tttaaagttt tgccttctcc atttagactt
5341 ataattcact ggaatttttt tgtgtgtatg gtatgacata tgggttccct tttatttttt
5401 acatataaat atatttccct gtttttctaa aaaagaaaaa gatcatcatt ttcccattgt
5461 aaaatgccat atttttttca taggtcactt acatatatca atgggtctgt ttctgagctc
5521 tactctattt tatcagcctc actgtctatc cccacacatc tcatgctttg ctctaaatct
5581 tgatatttag tggaacattc tttcccattt tgttctacaa gaatattttt gttattgtct
5641 tttgggcttc tatatacatt ttagaatgag gttggcaagt tctcgacctg cagccaagct
5701 tatcgataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca
5761 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaatacata
5821 actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga atatgggcca
5881 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
5941 cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
6001 aaggacctga aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
6061 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
6121 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
6181 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
6241 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
6301 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
6361 actccaatac tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat
6421 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
6481 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
6541 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
6601 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
6661 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
6721 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
6781 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
6841 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
6901 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
6961 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
7021 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
7081 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
7141 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
7201 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
7261 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
7321 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
7381 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
7441 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
7501 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
7561 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
7621 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
7681 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
7741 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
7801 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
7861 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
7921 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
7981 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
8041 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
8101 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
8161 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
8221 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
8281 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
8341 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
8401 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
8461 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
8521 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
8581 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
8641 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
8701 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
8761 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
8821 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
8881 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
8941 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
9001 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aagcagccca
9061 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
9121 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
9181 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
9241 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
9301 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
9361 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
9421 agaaaaaggg gggaa
//
