| 质粒名称: | pLV-EF1a-MinD-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 213114 |
| 存储实验室: | Scott Coyle |
|
载体骨架基本信息 |
|
| 载体名称: | pLV-EF1a |
| 载体出品公司: | Clontech |
| 空载体大小: | 8699bp |
| 完整质粒大小: | 9512bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MinD |
| 物种来源: | Escherichia coli |
| 插入大小: | 813bp |
| 启动子: | EF1a |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | EF1a_F (TCAAGCCTCAGACAGTGGTTC) |
| 3’测序引物 | EF1a_R (TAGAGGGAAACCGTTGCTAGC) |
|
文献引用方法 |
|
| 材料和方法部分: |
pLV-EF1a-MinD-IRES-Puro was a gift from Scott Coyle (Addgene plasmid # 213114 ; http://n2t.net/addgene:213114 ; RRID:Addgene_213114)
|
| 参考文献部分: | A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design. Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM. Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007. 10.1016/j.cell.2023.12.007 PubMed 38181787 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLV-EF1a-MinD-IRES-Puro 9512 bp ds-DNA circular SYN 26-JAN-2024
DEFINITION Expression of untagged MinD in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pLV-EF1a-MinD-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9512)
AUTHORS Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
TITLE A programmable reaction-diffusion system for spatiotemporal cell
signaling circuit design.
JOURNAL Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi:
10.1016/j.cell.2023.12.007.
PUBMED 38181787
REFERENCE 2 (bases 1 to 9512)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 26, 2024 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9512
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 83..294
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 295..1233
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1190..1210
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 1249..1258
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
primer_bind complement(2312..2329)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2539..2558
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2720..3319
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2720..2739)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3216..3236
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3343..3931
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3396..3416)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3814..3825)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4138..4771
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4899..4915)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4899..4915)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4912..4934)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4923..4939
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4947..4977)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4992..5013
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5130..5147)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5301..5889)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5381..5400)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6060..6920)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6683..6702
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6921..7025)
/gene="bla"
/label=AmpR promoter
polyA_signal 7073..7207
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7110..7129)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7164..7183
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 7375..8008
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 8055..8180
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8677..8910
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9095..9139
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 9402..5
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 aattttcggg tttattacag ggacagcaga gatccagttt atcgatgagg ccctttcgtc
61 ttcactcgag gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
121 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
181 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
241 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
301 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
361 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
421 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
481 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
541 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
601 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
661 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
721 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
781 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
841 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
901 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
961 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
1021 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1081 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1141 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1201 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatccgc caccatggcc
1261 cgcataatag ttgtgacttc aggtaagggc ggagttggaa aaaccacatc cagcgcagcc
1321 atagctaccg gcctcgcaca gaaaggcaag aaaactgtgg tcatagattt tgatattggg
1381 ctcaggaacc tggacctgat aatgggctgc gaacggcgcg ttgtctacga ttttgtcaat
1441 gtcatccaag gcgacgcgac tcttaatcag gcacttataa aagataaacg aaccgaaaac
1501 ttgtatattc ttcctgcctc acaaacgcgg gacaaagacg cgctcaccag ggagggtgtc
1561 gcaaaagttt tggatgactt gaaagcgatg gactttgagt tcatagtttg tgactctccc
1621 gccggtatag agacaggggc acttatggca ctgtacttcg cagatgaagc cataatcaca
1681 actaatccgg aggtgtctag tgtgcgagat tcagaccgaa tactggggat actcgccagc
1741 aaatcacgga gggcagaaaa cggggaggag ccaattaagg agcatctcct gcttacccga
1801 tataaccccg gtcgagtatc tcggggagat atgctttcaa tggaagatgt cctcgagatt
1861 ctgaggataa aactggtagg cgttatcccg gaagaccagt ccgtcctccg cgcatctaat
1921 cagggggagc ctgtcatact tgacataaat gcggatgctg ggaaagctta tgccgatacg
1981 gtagaacggc tcctcgggga agaacggccc ttccgcttca tagaggagga aaagaaggga
2041 ttccttaaac ggctgttcgg gggataagcg gccgctctag agtcgacggg ccgcggtaac
2101 aattgttaac taacttaagc tagcaacggt ttccctctag cgggatcaat tccgcccccc
2161 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
2221 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
2281 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
2341 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
2401 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
2461 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
2521 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
2581 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
2641 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
2701 tgaaaaacac gataatacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
2761 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
2821 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
2881 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
2941 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
3001 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
3061 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
3121 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
3181 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
3241 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
3301 ccgcaagccc ggtgcctgac ccgggcgggg cgcgtctgga acaatcaacc tctggattac
3361 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
3421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
3481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
3541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
3601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
3661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
3721 gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg
3781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
3841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
3901 agtcggatct ccctttgggc cgcctccccg cctggaatta attctgcagt cgagacctag
3961 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
4021 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
4081 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg
4141 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
4201 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
4261 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
4321 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
4381 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
4441 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
4501 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
4561 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
4621 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
4681 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
4741 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
4801 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt
4861 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
4921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
4981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
5041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
5101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
5161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
5221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
5281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
5341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
5401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
5461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
5521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
5581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
5641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
5701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
5761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
5821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
5881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
5941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
6001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
6061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
6121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
6181 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
6241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
6301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
6361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
6421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
6481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
6541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
6601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
6661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
6721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
6781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
6841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
6901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
6961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
7021 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
7081 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
7141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
7201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
7261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
7321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
7381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
7441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
7501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
7561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
7621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
7681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
7741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
7801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
7861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
7921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
7981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
8041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
8101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
8161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
8221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
8281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
8341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
8401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
8461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
8521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
8581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
8641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
8701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
8761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
8821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
8881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
8941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
9001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
9061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
9121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
9181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
9241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
9301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
9361 cagatccatt cgattagtga acggatctcg acggtatcgc ctttaaaaga aaagggggga
9421 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
9481 aagaattaca aaaacaaatt acaaaaattc aa
//
