质粒名称: | pAAVS1-P-CAG-DEST |
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出品公司: | Addgene |
目录编号: | 80490 |
存储实验室: | Knut Woltjen |
载体骨架基本信息 |
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载体名称: | pBluescript |
载体出品公司: | -- |
空载体大小: | 3000 bp |
完整质粒大小: | -- |
载体修饰: | AAVS1的同源臂;无启动子SA-T2A-puro选择盒;CAG启动子;网关目的磁带 |
载体类型: | 哺乳动物表达;供体载体(人类) |
筛选标记: | 嘌呤霉素 |
载体抗性: | 氯霉素和氨苄青霉素 |
生长温度: | 37 ℃ |
宿主菌: | ccdB Survival |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | -- |
标签/融合蛋白: | -- |
克隆方法: | Gateway克隆 |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pAAVS1-P-CAG-DEST was a gift from Knut Woltjen (Addgene plasmid # 80490 ; http://n2t.net/addgene:80490 ; RRID:Addgene_80490) |
参考文献部分: | Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K. Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. 10.1016/j.ymeth.2015.12.012 PubMed 26707206 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAVS1-P-CAG-DEST 9502 bp ds-DNA circular SYN 09-SEP-2021 DEFINITION Gateway Destination donor vector for AAVS1 targeting (puromycin selection). ACCESSION . VERSION . KEYWORDS pAAVS1-P-CAG-DEST SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9502) AUTHORS Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K TITLE Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives. JOURNAL Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 10.1016/j.ymeth.2015.12.012. PUBMED 26707206 REFERENCE 2 (bases 1 to 9502) AUTHORS . TITLE Direct Submission JOURNAL Exported Sep 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9502 /organism="synthetic DNA construct" /mol_type="other DNA" intron 105..1121 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1045..1067 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1129..1148 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" protein_bind 1215..1339 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1364..1394 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1448..2107 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1514..1533) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2449..2754 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2633..2652 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2795..2919) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(3015..3034) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3080..3135 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3134..3153) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" misc_feature 3691..4220 /label=HA-R /note="right homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" primer_bind complement(4366..4385) /label=T7 /note="T7 promoter, forward primer" promoter complement(4367..4385) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(4392..4409) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(4392..4408) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4401..4423) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 4550..5005 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4637..4656) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4847..4868 /label=F1ori-F /note="F1 origin, forward primer" promoter 5031..5135 /gene="bla" /label=AmpR promoter CDS 5136..5996 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(5354..5373) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 6167..6755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6656..6675 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6909..6926 /label=L4440 /note="L4440 vector, forward primer" protein_bind 7043..7064 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 7079..7109 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7117..7133 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 7122..7144 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 7141..7157 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 7141..7157 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 7178..7196 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" misc_feature 7209..8012 /label=HA-L /note="left homology arm from the adeno-associated virus integration site (AAVS1) within intron 1 of the human PPP1R12C gene" misc_feature 8019..8044 /label=SA /note="splice acceptor site" CDS 8068..8121 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 8131..8730 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(8131..8150) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 8627..8647 /label=Puro-F /note="Puromycin resistance gene, forward primer" primer_bind complement(8762..8779) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8768..8992 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" enhancer 9011..9390 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc 61 ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct 121 gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga 181 ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc 241 gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc 301 gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg 361 ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg 421 ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt 481 gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag 541 cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct ccccgagttg 601 ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg 661 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg 721 gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg 781 cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt 841 cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg 901 gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc 961 cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg gacggctgcc 1021 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag 1081 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg 1141 ttattgtgct gtctcatcat tttggcaaag aattcgcggc cgcggtaccg gcgcgccgga 1201 tcctgcaggt tatcacaagt ttgtacaaaa aagctgaacg agaaacgtaa aatgatataa 1261 atatcaatat attaaattag attttgcata aaaaacagac tacataatac tgtaaaacac 1321 aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac tttatgcttc 1381 cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga 1441 agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg 1501 taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca 1561 gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc 1621 ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa 1681 agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca 1741 aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca 1801 catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt 1861 tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt 1921 aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac 1981 gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg 2041 cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg 2101 ggcgtaaacg cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc 2161 tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg 2221 tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag 2281 gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc 2341 gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt 2401 ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag 2461 gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt 2521 attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat 2581 aaagtctccc gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg 2641 accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc 2701 caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca 2761 ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat gttgtgtttt 2821 acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc 2881 attttacgtt tctcgttcag ctttcttgta caaagtggtg ataactctag agaattcact 2941 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 3001 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 3061 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 3121 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 3181 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 3241 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 3301 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 3361 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 3421 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 3481 cttggatccc tcgagttaat taaatctaga agtcgacagt actaagcttt gacagaaaag 3541 ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct 3601 gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg 3661 ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg 3721 gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg 3781 cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat 3841 cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc 3901 agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata 3961 ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg 4021 cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg 4081 tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt 4141 tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc 4201 ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg 4261 ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc 4321 ttccctacag gggttcctgg ctctgctcta gcgatcgcca attcgcccta tagtgagtcg 4381 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc 4441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc 4501 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt 4561 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 4621 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 4681 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 4741 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 4801 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 4861 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4921 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 4981 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc 5041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 5101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 5161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 5221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 5281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 5341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 5401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 5461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 5521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 5581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 5641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 5701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 5761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 5821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 5881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 5941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 6001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 6061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 6121 cgttccactg agcgtcaaac cccgtagaaa agatcaaagg atcttcttga gatccttttt 6181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 6241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 6301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 6361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 6421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 6481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 6541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 6601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 6661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 6721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 6781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 6841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 6901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 6961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 7021 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 7081 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 7141 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac 7201 aaaagctgtg ctttctctga ccagcattct ctcccctggg cctgtgccgc tttctgtctg 7261 cagcttgtgg cctgggtcac ctctacggct ggcccagatc cttccctgcc gcctccttca 7321 ggttccgtct tcctccactc cctcttcccc ttgctctctg ctgtgttgct gcccaaggat 7381 gctctttccg gagcacttcc ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc 7441 tccccgtgtc tgggtcctct ccgggcatct ctcctccctc acccaacccc atgccgtctt 7501 cactcgctgg gttccctttt ccttctcctt ctggggcctg tgccatctct cgtttcttag 7561 gatggccttc tccgacggat gtctcccttg cgtcccgcct ccccttcttg taggcctgca 7621 tcatcaccgt ttttctggac aaccccaaag taccccgtct ccctggcttt agccacctct 7681 ccatcctctt gctttctttg cctggacacc ccgttctcct gtggattcgg gtcacctctc 7741 actcctttca tttgggcagc tcccctaccc cccttacctc tctagtctgt gctagctctt 7801 ccagccccct gtcatggcat cttccagggg tccgagagct cagctagtct tcttcctcca 7861 acccgggccc ctatgtccac ttcaggacag catgtttgct gcctccaggg atcctgtgtc 7921 cccgagctgg gaccacctta tattcccagg gccggttaat gtggctctgg ttctgggtac 7981 ttttatctgt cccctccacc ccacagtggg gcaagcttct gacctcttct cttcctccca 8041 cagggcctcg agagatctgg cagcggagag ggcagaggaa gtcttctaac atgcggtgac 8101 gtggaggaga atcccggccc taggctcgag atgaccgagt acaagcccac ggtgcgcctc 8161 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac 8221 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa 8281 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc 8341 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag 8401 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa 8461 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc 8521 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg 8581 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc 8641 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc 8701 tggtgcatga cccgcaagcc cggtgcctga tctagagggc ccgtttaaac ccgctgatca 8761 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc 8821 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg 8881 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg 8941 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggactagcgg 9001 tggcggcctc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta 9061 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 9121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 9181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg 9241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 9301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 9361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact 9421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt 9481 tgtgcagcga tgggggcggg gg //
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