| 质粒名称: | pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 85431 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR SIN |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 12457 bp |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | human Tbet |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry was a gift from Wendell Lim (Addgene plasmid # 85431 ; http://n2t.net/addgene:85431 ; RRID:Addgene_85431) |
| 参考文献部分: | Engineering T Cells with Customized Therapeutic Response Programs Using Synthetic Notch Receptors. Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, Walker WJ, McNally KA, Lim WA. Cell. 2016 Oct 6;167(2):419-432.e16. doi: 10.1016/j.cell.2016.09.011. Epub 2016 Sep 29. 10.1016/j.cell.2016.09.011 PubMed 27693353 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry 12457 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive
mCherry expression.
ACCESSION .
VERSION .
KEYWORDS pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12457)
AUTHORS Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH,
Walker WJ, McNally KA, Lim WA
TITLE Engineering T Cells with Customized Therapeutic Response Programs
Using Synthetic Notch Receptors.
JOURNAL Cell. 2016 Oct 6;167(2):419-432.e16. doi:
10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
PUBMED 27693353
REFERENCE 2 (bases 1 to 12457)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..12457
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1612..1665
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 1672..2388
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 1672..2385
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(1714..1735)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1975..1994)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2322..2343
/label=EGFP-C
/note="EGFP, forward primer"
promoter 2511..3010
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2546..2569)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2943..2962
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3031..3741
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(3176..3194)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 3444..3463
/label=mCherry-F
/note="mCherry, forward primer"
misc_feature 3818..4406
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3871..3891)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4289..4300)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4409..4425)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4410..4426)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 4516..4749
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(4774..4792)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4775..4792)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(5082..5101)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(5315..5334)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5434..5456
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5494..5512)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5580..5684
/gene="bla"
/label=AmpR promoter
CDS 5685..6545
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5903..5922)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6716..7304
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7205..7224
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7458..7475
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7545..7565)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7550..7879
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7730..7865
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7792..7811
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 9013..9078
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 9208..9228
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9653..9787
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9690..9709)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9744..9763
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 9955..10588
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 10635..10760
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 11257..11490
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 11675..11719
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 12004..12121
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 12347..12371
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 12452..4
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
ORIGIN
1 atgggcatcg tggagccggg ttgcggagac atgctgacgg gcaccgagcc gatgccgggg
61 agcgacgagg gccgggcgcc tggcgccgac ccgcagcacc gctacttcta cccggagccg
121 ggcgcgcagg acgcggacga gcgtcgcggg ggcggcagcc tggggtctcc ctacccgggg
181 ggcgccttgg tgcccgcccc gccgagccgc ttccttggag cctacgccta cccgccgcga
241 ccccaggcgg ccggcttccc cggcgcgggc gagtccttcc cgccgcccgc ggacgccgag
301 ggctaccagc cgggcgaggg ctacgccgcc ccggacccgc gcgccgggct ctacccgggg
361 ccgcgtgagg actacgcgct acccgcggga ctggaggtgt cggggaaact gagggtcgcg
421 ctcaacaacc acctgttgtg gtccaagttt aatcagcacc agacagagat gatcatcacc
481 aagcagggac ggcggatgtt cccattcctg tcatttactg tggccgggct ggagcccacc
541 agccactaca ggatgtttgt ggacgtggtc ttggtggacc agcaccactg gcggtaccag
601 agcggcaagt gggtgcagtg tggaaaggcc gagggcagca tgccaggaaa ccgcctgtac
661 gtccacccgg actcccccaa cacaggagcg cactggatgc gccaggaagt ttcatttggg
721 aaactaaagc tcacaaacaa caagggggcg tccaacaatg tgacccagat gattgtgctc
781 cagtccctcc ataagtacca gccccggctg catatcgttg aggtgaacga cggagagcca
841 gaggcagcct gcaacgcttc caacacgcat atctttactt tccaagaaac ccagttcatt
901 gccgtgactg cctaccagaa tgccgagatt actcagctga aaattgataa taaccccttt
961 gccaaaggat tccgggagaa ctttgagtcc atgtacacat ctgttgacac cagcatcccc
1021 tccccgcctg gacccaactg tcaattcctt gggggagatc actactctcc tctcctaccc
1081 aaccagtatc ctgttcccag ccgcttctac cccgaccttc ctggccaggc gaaggatgtg
1141 gttccccagg cttactggct gggggccccc cgggaccaca gctatgaggc tgagtttcga
1201 gcagtcagca tgaagcctgc attcttgccc tctgcccctg ggcccaccat gtcctactac
1261 cgaggccagg aggtcctggc acctggagct ggctggcctg tggcacccca gtaccctccc
1321 aagatgggcc cggccagctg gttccgccct atgcggactc tgcccatgga acccggccct
1381 ggaggctcag agggacgggg accagaggac cagggtcccc ccttggtgtg gactgagatt
1441 gcccccatcc ggccggaatc cagtgattca ggactgggcg aaggagactc taagaggagg
1501 cgcgtgtccc cctatccttc cagtggtgac agctcctccc ctgctggggc cccttctcct
1561 tttgataagg aagctgaagg acagttttat aactattttc ccaacaccgg tgagggcaga
1621 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gcccttccgg ggtgagcaag
1681 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
1741 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
1801 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
1861 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
1921 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
1981 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
2041 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
2101 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
2161 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
2221 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
2281 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
2341 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaga tccttgactt
2401 gcggccgcaa ctcccacctg caacatgcgt gactgactga ggccgcgact ctagagtcga
2461 cctgcatcta ggcgccggaa ttagatctct cgaggttaac gaattctacc gggtagggga
2521 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
2581 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
2641 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
2701 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
2761 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
2821 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
2881 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
2941 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
3001 gggcctttcg acctgcagcc caagcttacc atggtgagca agggcgagga ggataacatg
3061 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
3121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
3181 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
3241 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
3301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
3361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
3421 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
3481 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
3541 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
3601 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
3661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
3721 atggacgagc tgtacaagta aagcggccgc gactctagag tcgacctgca ggcatgcaag
3781 cttgcaggca tgcaagcttg atatcaagct tatcgataat caacctctgg attacaaaat
3841 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
3901 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
3961 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
4021 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
4081 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
4141 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
4201 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
4261 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
4321 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
4381 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgaggga attaattcga
4441 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
4501 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
4561 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
4621 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
4681 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
4741 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
4801 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
4861 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
4921 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
4981 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
5041 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
5101 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
5161 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
5221 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
5281 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
5341 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
5401 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
5461 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
5521 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
5581 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
5641 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
5701 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
5761 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
5821 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
5881 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
5941 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
6001 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
6061 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
6121 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
6181 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
6241 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
6301 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
6361 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
6421 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
6481 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
6541 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
6601 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
6661 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
6721 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
6781 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
6841 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
6901 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
6961 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
7021 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
7081 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
7141 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
7201 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
7261 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
7321 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
7381 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
7441 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
7501 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt
7561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
7621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
7681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
7741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
7801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
7861 aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa
7921 taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa
7981 tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca
8041 ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca
8101 cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac
8161 aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg
8221 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
8281 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
8341 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
8401 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
8461 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
8521 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
8581 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
8641 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
8701 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
8761 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg
8821 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
8881 tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc
8941 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
9001 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
9061 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
9121 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
9181 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
9241 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
9301 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
9361 gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt
9421 tttcttactc cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt
9481 acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg
9541 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
9601 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
9661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
9721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
9781 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
9841 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
9901 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
9961 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
10021 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
10081 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
10141 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
10201 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
10261 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
10321 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
10381 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
10441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
10501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
10561 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
10621 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
10681 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
10741 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
10801 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
10861 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
10921 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
10981 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
11041 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
11101 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
11161 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
11221 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
11281 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
11341 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
11401 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
11461 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
11521 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
11581 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
11641 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
11701 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
11761 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
11821 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
11881 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
11941 cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag tattcatcca
12001 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
12061 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
12121 tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg atatcgaatt
12181 cggagcactg tcctccgaac gtcggagcac tgtcctccga acgtcggagc actgtcctcc
12241 gaacgtcgga gcactgtcct ccgaacggag catgtcctcc gaacgtcgga gcactgtcct
12301 ccgaacgact agttaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
12361 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
12421 accgatccag cctctcgaca ttcgttggat cgccacc
//
