| 出品公司: | CAMBIA |
|---|---|
| 载体名称: | pCAMBIA1300 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | Lac |
| 载体大小: | 8959 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素（Hygromycin） |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pcambia1300载体质粒基本信息
pcambia1300质粒图谱载体图谱和pcambia1300载体序列质粒序列多克隆位点信息
pcambia1300质粒载体简介
pcambia1300质粒序列载体序列
LOCUS AF234296 8958 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1300, complete sequence.
ACCESSION AF234296
VERSION AF234296.1 GI:7638064
KEYWORDS .
SOURCE Binary vector pCAMBIA-1300
ORGANISM Binary vector pCAMBIA-1300
other sequences; artificial sequences; vectors.
REFERENCE 1 (sites)
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
for plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 8958)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REMARK Full description of constructs
REFERENCE 3 (bases 1 to 8958)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..8958
/organism="Binary vector pCAMBIA-1300"
/mol_type="other DNA"
/db_xref="taxon:118388"
CDS join(8944..8958,1..219)
/codon_start=1
/product="LacZ alpha fragment"
/protein_id="AAF65336.1"
/db_xref="GI:7638065"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLN
RLAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 28..78
/note="pUC18 MCS; polylinker"
misc_feature 298..323
/note="right border T-DNA repeat"
misc_feature complement(1364..2364)
/note="STA region from pVS1 plasmid"
rep_origin complement(2957..3957)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(4367..4627)
/note="bom site from pBR322"
rep_origin complement(4767..5047)
/note="pBR322 origin of replication"
CDS complement(5338..6132)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65338.1"
/db_xref="GI:7638067"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6557..6582
/note="left border repeat from C58 T-DNA"
misc_feature 6649..6857
/note="CaMV 3'UTR (polyA signal)"
CDS complement(6873..7898)
/note="hptII (hygromycin resistance) gene"
/codon_start=1
/product="hygromycin phosphotransferase"
/protein_id="AAF65337.1"
/db_xref="GI:7638066"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK"
promoter complement(7934..8714)
/note="CaMV35S; 35S promoter from CaMV"
promoter 8862..8916
/note="PlacZ; lacZ promoter"
ORIGIN
1 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
61 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
121 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
181 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
241 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
301 aacctaagag aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt
361 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
421 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
481 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
541 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
601 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
661 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
721 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
781 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
841 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
901 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
961 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
1021 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
1081 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
1141 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
1201 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
1261 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
1321 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
1381 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
1441 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
1501 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
1561 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
1621 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
1681 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
1741 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
1801 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
1861 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
1921 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
1981 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
2041 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
2101 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
2161 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
2221 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
2281 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
2341 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
2401 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
2461 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
2521 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
2581 ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc
2641 cccaagcccg aggaatcggc gtgacggtcg caaaccatcc ggcccggtac aaatcggcgc
2701 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
2761 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
2821 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
2881 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
2941 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
3001 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
3061 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
3121 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
3181 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
3241 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
3301 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
3361 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
3421 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
3481 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
3541 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
3601 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
3661 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
3721 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
3781 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
3841 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
3901 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
3961 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
4021 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
4081 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
4141 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
4201 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
4261 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
4321 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
4381 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
4441 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
4501 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
4561 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
4621 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
4681 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
4741 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
4801 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
4861 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
4921 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
4981 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
5041 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
5101 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
5161 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
5221 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
5281 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta
5341 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
5401 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
5461 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
5521 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
5581 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
5641 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
5701 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
5761 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
5821 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
5881 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
5941 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
6001 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
6061 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
6121 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
6181 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
6241 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
6301 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
6361 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
6421 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
6481 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
6541 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
6601 ttaataacac attgcggacg tttttaatgt actgaattaa cgccgaatta attcggggga
6661 tctggatttt agtactggat tttggtttta ggaattagaa attttattga tagaagtatt
6721 ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag cgaaacccta
6781 taggaaccct aattccctta tctgggaact actcacacat tattatggag aaactcgagc
6841 ttgtcgatcg acagatccgg tcggcatcta ctctatttct ttgccctcgg acgagtgctg
6901 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
6961 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
7021 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
7081 tggtcaagac caatgcggag catatacgcc cggagtcgtg gcgatcctgc aagctccgga
7141 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
7201 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
7261 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
7321 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
7381 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
7441 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
7501 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatagcctc cgcgaccggt
7561 tgtagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
7621 cgggagatgc aataggtcag gctctcgcta aactccccaa tgtcaagcac ttccggaatc
7681 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
7741 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
7801 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
7861 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatct
7921 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
7981 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
8041 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
8101 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
8161 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
8221 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
8281 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
8341 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
8401 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
8461 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
8521 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
8581 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
8641 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
8701 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
8761 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
8821 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
8881 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
8941 gctatgacca tgattacg
//
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