质粒名称: | pNH8 |
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出品公司: | 爱迪基因 |
目录编号: | 40859 |
存储实验室: | Adam Bogdanove |
载体骨架基本信息 |
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载体名称: | pTC14 |
载体抗性: | 四环素 |
空载体大小: | 3013 bp |
完整质粒大小: | 3113 bp |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | repeat NH8 |
物种来源: | Xanthomonas oryzae |
插入大小: | 100 bp |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | XbaI(未破坏) |
3' 克隆位点: | XhoI (未破坏) |
5' 测序引物 | pTC14_F2 (caagcctgattgggagaaaa) |
文献引用方法 |
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材料和方法部分: | pNH8 was a gift from Adam Bogdanove (Addgene plasmid # 40859 ; http://n2t.net/addgene:40859 ; RRID:Addgene_40859) |
参考文献部分: | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNH8 3113 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pNH8 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3113) AUTHORS Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF TITLE Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. JOURNAL Nucleic Acids Res. 2011 Apr 14. ():. PUBMED 21493687 REFERENCE 2 (bases 1 to 3113) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3113 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 196..224 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 272..1462 /codon_start=1 /gene="tet" /product="tetracycline efflux protein" /label=TcR /note="confers resistance to tetracycline" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST" primer_bind complement(624..643) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(1636..1655) /label=T7 /note="T7 promoter, forward primer" promoter complement(1637..1655) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1660..1676) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1660..1676) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" rep_origin 1962..2550 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2451..2470 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 2880..2907 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 2999..3085 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca 241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg 361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc 421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt 481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat 541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg 601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa 781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg 841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc 1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg 1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct 1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga 1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat 1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact 1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga 1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg 1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag 1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca 1741 tgcctcctct agaggtctcc gaaacggtgc agcggctgtt gccggtgctg tgccaggacc 1801 atggcctgac cccggaccaa gtggtggcta tcgccagcaa tcacggcggc aagcaagcgc 1861 tcgaagagac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc 1921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 1981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc 2761 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc 2821 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa 2881 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc 2941 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat 3001 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt 3061 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gtt //