| 出品公司: | Clontech |
|---|---|
| 载体名称: | pRetroX-TetOne |
| 质粒类型: | 逆病毒载体；四环素调控载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TRE3GS , hPGK |
| 载体大小: | 8508 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 逆病毒载体pRetroX-TetOne是四环素调控系统载体；与其它四环素诱导系统不同，pRetroX-TetOne集调控功能与应答功能于一体。 |
| 产品目录号: | 634306 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pRetroX-TetOne载体质粒基本信息
pRetroX-TetOne质粒图谱载体图谱和pRetroX-TetOne载体序列质粒序列多克隆位点信息
pRetroX-TetOne质粒载体简介
pRetroX-TetOne质粒序列载体序列
LOCUS pRetroX-TetOne 8508 bp ds-DNA circular SYN 23-SEP-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Catalog Nos.634304, 634305, 634306
FEATURES Location/Qualifiers
LTR 376..911
/label="CMV/MLV 5'LTR"
misc_feature 974..1331
/label="Packaging signal"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
polyA_signal 2298..2432
/label="SV40 polyA signal"
misc_feature 2607..2638
/label="MCS"
promoter complement(2639..3003)
/label="TRE3GS promoter"
promoter 3023..3533
/label="hPGK promoter"
CDS 3552..4298
/label="Tet-On 3G"
misc_feature 4316..4724
/label="WPRE"
/note="Partial WPRE. Contains alpha and gamma elements but no
WHV protein X coding sequence"
LTR 4845..5092
/label="3'-LTR del in U3"
promoter 6370..6474
/label="AmpR promoter"
CDS 6475..7335
/label="AmpR"
rep_origin 7506..8094
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
61 tgacttgttc tatgcgccta ggagcttggc ccattgcata cgttgtatcc atatcataat
121 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
361 tgggtggagt atttacgcta aactgcccac ttggcagtac atcaagtgta tcatatgcca
421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcat ctagagcgct
721 tctgctcccc gagctctata aaagagccca caacccctca ctcggggcgc cagtcctccg
781 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
841 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
901 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
961 accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta
1021 tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga
1081 cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg
1141 acttcggggg ccgtttttgt ggcccgacct gagtcctaaa atcccgatcg tttaggactc
1201 tttggtgcac cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa
1261 cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt
1321 cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
1381 gaaaatgagg gcccgggcta gcctgttacc actcccttaa gtttgacctt aggtcactgg
1441 aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt
1501 accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt
1561 aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac
1621 ccagaccagg tggggtacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg
1681 gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc
1741 ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct
1801 tctctaggcg cccccatatg gccatatgag atcctatatg gggcaccccc gccccttgta
1861 aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca agctcactta
1921 caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa
1981 caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc
2041 cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc
2101 acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag
2161 gctgccgacc ccgggggtgg accatcctct agatcataat cagccatacc acatttgtag
2221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
2281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata
2341 gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca
2401 aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtct
2461 taggttggag tgatacattt ttatcacttt tacccgtctt tggattaggc agtagctctg
2521 acggccctcc tgtcttaggt tagtgaaaaa tgtcactctc ttacccgtca ttggctgtcc
2581 agcttagctc gcaggggagg tggtctggat ccgccggcac cggtgtatac gggaattctt
2641 tacgagggta ggaagtggta cggaaagttg gtataagaca aaagtgttgt ggaattgaag
2701 tttactcaaa aaatcagcac tcttttatag gcgccctggt ttacataagc aaagcttata
2761 cgttctctat cactgatagg gagtaaactg gatatacgtt ctctatcact gatagggagt
2821 aaactgtaga tacgttctct atcactgata gggagtaaac tggtcatacg ttctctatca
2881 ctgataggga gtaaactcct tatacgttct ctatcactga tagggagtaa agtctgcata
2941 cgttctctat cactgatagg gagtaaactc ttcatacgtt ctctatcact gatagggagt
3001 aaactcgagg tgataattcc acggggttgg ggttgcgcct tttccaaggc agccctgggt
3061 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
3121 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
3181 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
3241 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
3301 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
3361 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
3421 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
3481 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggatc
3541 atcgaattac catgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
3601 tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
3661 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctgccaa
3721 tcgagatgct ggacaggcat catacccact cctgccccct ggaaggcgag tcatggcaag
3781 actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat cgcgacgggg
3841 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
3901 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
3961 tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta gcaaaagagg
4021 aaagagagac acctaccacc gattctatgc ccccacttct gaaacaagca attgagctgt
4081 tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
4141 tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac gattttgact
4201 tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg cctgctgacg
4261 ctcttgacga ttttgacctt gacatgctcc ccgggtaact aagtaagatc ctgttaatca
4321 acctctggat tacaaaattt gtgaaagatt gactgatatt cttaactatg ttgctccttt
4381 tacgctgtgt ggatatgctg ctttaatgcc tctgtatcat gctattgctt cccgtacggc
4441 tttcgttttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
4501 cgttgtccgt caacgtggcg tggtgtgctc tgtgtttgct gacgcaaccc ccactggctg
4561 gggcattgcc accacctgtc aactcctttc tgggactttc gctttccccc tcccgatcgc
4621 cacggcagaa ctcatcgccg cctgccttgc ccgctgctgg acaggggcta ggttgctggg
4681 cactgataat tccgtggtgt tgtcggggaa gctgacgtcg attcctgcgg agatatcagt
4741 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
4801 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
4861 ctgtaggttt ggcaagctag cttctcgctt ctgttcgcgc gcttctgctc ccagacatca
4921 acaaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg
4981 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
5041 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cacgcgtatg
5101 ccgcatagtt aagccaccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
5161 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
5221 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
5281 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
5341 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
5401 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
5461 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac
5521 ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag ccgacccggg
5581 cggccccgcc gaccccgcac ccgcccccga ggcccaccga ctctagagga tcataatcag
5641 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
5701 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
5761 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
5821 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ggcgcgtcga attaattcac
5881 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
5941 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
6001 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
6061 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
6121 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
6181 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
6241 agaggttttc accgtcatca ccgaaacgcg cgatgacgaa agggcctcgt gatacgccta
6301 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
6361 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
6421 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
6481 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
6541 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
6601 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
6661 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
6721 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
6781 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
6841 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
6901 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
6961 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
7021 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
7081 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
7141 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
7201 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
7261 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
7321 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
7381 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
7441 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
7501 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
7561 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
7621 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
7681 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
7741 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
7801 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
7861 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc
7921 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
7981 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
8041 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
8101 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
8161 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
8221 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
8281 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
8341 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
8401 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
8461 agcggataac aatttcacac aggaaacagc tatgaccatg attacgcc
//
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