| 出品公司: | Clontech |
|---|---|
| 载体名称: | pRetroX-TetOne-luc |
| 质粒类型: | 逆病毒载体；四环素调控载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV IE |
| 载体大小: | 10144 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | DH5α |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | 逆病毒载体pRetroX-TetOne-luc是四环素诱导载体，作为pRetroX-TetOne载体的阳性参照。 |
| 产品目录号: | 634306 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pRetroX-TetOne-luc载体质粒基本信息
pRetroX-TetOne-luc质粒图谱载体图谱和pRetroX-TetOne-luc载体序列质粒序列多克隆位点信息
pRetroX-TetOne-luc质粒载体简介
pRetroX-TetOne-luc质粒序列载体序列
LOCUS pRetroX-TetOne-Luc 10144 bp ds-DNA circular SYN 23-SEP-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Catalog Nos. 634304, 634305, 634306
FEATURES Location/Qualifiers
LTR 376..911
/label="CMV/MLV 5'LTR"
misc_feature 974..1331
/label="Packaging Signal"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
polyA_signal 2298..2432
/label="SV40 polyA signal"
CDS complement(2613..4265)
/label="luciferase"
/note="firefly luciferase"
promoter complement(4275..4639)
/label="TRE3GS Promoter"
promoter 4659..5169
/label="hPGK promoter"
CDS 5188..5934
/label="Tet-On 3G"
misc_feature 5952..6360
/label="WPRE"
/note="Partial WPRE. Contains alpha and gamma elements but
no WHV protein X coding sequence"
LTR 6481..6728
/label="3'-LTR del in U3"
promoter 8006..8110
/label="AmpR promoter"
CDS 8111..8971
/label="AmpR"
rep_origin 9142..9730
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 aagctagctt tgctcttagg agtttcctaa tacatcccaa actcaaatat ataaagcatt
61 tgacttgttc tatgcgccta ggagcttggc ccattgcata cgttgtatcc atatcataat
121 atgtacattt atattggctc atgtccaaca ttaccgccat gttgacattg attattgact
181 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
241 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
301 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
361 tgggtggagt atttacgcta aactgcccac ttggcagtac atcaagtgta tcatatgcca
421 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
481 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
541 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
601 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
661 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcat ctagagcgct
721 tctgctcccc gagctctata aaagagccca caacccctca ctcggggcgc cagtcctccg
781 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac
841 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg
901 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc
961 accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt ctagtgtcta
1021 tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt atctggcgga
1081 cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga cgtcccaggg
1141 acttcggggg ccgtttttgt ggcccgacct gagtcctaaa atcccgatcg tttaggactc
1201 tttggtgcac cccccttaga ggagggatat gtggttctgg taggagacga gaacctaaaa
1261 cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg cgccgcgcgt
1321 cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct
1381 gaaaatgagg gcccgggcta gcctgttacc actcccttaa gtttgacctt aggtcactgg
1441 aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt
1501 accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt
1561 aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac
1621 ccagaccagg tggggtacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg
1681 gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc
1741 ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct
1801 tctctaggcg cccccatatg gccatatgag atcctatatg gggcaccccc gccccttgta
1861 aacttccctg accctgacat gacaagagtt actaacagcc cctctctcca agctcactta
1921 caggctctct acttagtcca gcacgaagtc tggagacctc tggcggcagc ctaccaagaa
1981 caactggacc gaccggtggt acctcaccct taccgagtcg gcgacacagt gtgggtccgc
2041 cgacaccaga ctaagaacct agaacctcgc tggaaaggac cttacacagt cctgctgacc
2101 acccccaccg ccctcaaagt agacggcatc gcagcttgga tacacgccgc ccacgtgaag
2161 gctgccgacc ccgggggtgg accatcctct agatcataat cagccatacc acatttgtag
2221 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
2281 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaggcaata
2341 gcatcacaaa tttcacaaat aaggcatttt tttcactgca ttctagtttt ggtttgtcca
2401 aactcatcaa tgtatcttat catgtctgga tctcaaatcc ctcggaagct gcgcctgtct
2461 taggttggag tgatacattt ttatcacttt tacccgtctt tggattaggc agtagctctg
2521 acggccctcc tgtcttaggt tagtgaaaaa tgtcactctc ttacccgtca ttggctgtcc
2581 agcttagctc gcaggggagg tggtctggat ccttacaatt tggactttcc gcccttcttg
2641 gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag acctttcggt
2701 acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac ttgactggcg
2761 acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac aacaacggcg
2821 gcgggaagtt caccggcgtc atcgtcggga agacctgcca cgcccgcgtc gaagatgttg
2881 gggtgttgta acaatatcga ctccaattca gcgggggcca cctggtatcc tttgtattta
2941 attaaagact tcaagcggtc aactatgaag aagtgttcgt cttcgtccca gtaagctatg
3001 tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc cggattgttt
3061 acataaccgg acataatcat aggtcctctg acacataatt cgcctctctg attaacgccc
3121 agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac aactttaccg
3181 accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt agtctcagtg
3241 agcccatatc cttgtcgtat ccctggaaga tggaagcgtt ttgcaaccgc ttccccgact
3301 tctttcgaaa gaggtgcgcc cccagaagca atttcgtgta aattagataa atcgtatttg
3361 tcaatcagag tgcttttggc gaagaatgaa aatagggttg gtactagcaa cgcactttga
3421 attttgtaat cctgaaggga tcgtaaaaac agctcttctt caaatctata cattaagacg
3481 actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc gtgatggaat
3541 ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat aggatctctg
3601 gcatgcgaga atctgacgca ggcagttcta tgcggaaggg ccacaccctt aggtaaccca
3661 gtagatccag aggagttcat tatcagtgca attgttttgt cacgatcaaa ggactctggt
3721 acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt gtacatcgac
3781 tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttctggat tattggtaat
3841 tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacaaacac tacggtaggc
3901 tgcgaaatgt tcatactgtt gagcaattca cgttcattat aaatgtcgtt cgcgggcgca
3961 actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag agagttttca
4021 ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc ttctgccaac
4081 cgaacggaca tttcgaagta ttccgcgtac gtgatgttca cctcgatatg tgcatctgta
4141 aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag ttgctctcca
4201 gcggttccat cctcaagagg atagaatggc gccgggcctt tctttatgtt tttggcgtct
4261 tccatggtga attctttacg agggtaggaa gtggtacgga aagttggtat aagacaaaag
4321 tgttgtggaa ttgaagttta ctcaaaaaat cagcactctt ttataggcgc cctggtttac
4381 ataagcaaag cttatacgtt ctctatcact gatagggagt aaactggata tacgttctct
4441 atcactgata gggagtaaac tgtagatacg ttctctatca ctgataggga gtaaactggt
4501 catacgttct ctatcactga tagggagtaa actccttata cgttctctat cactgatagg
4561 gagtaaagtc tgcatacgtt ctctatcact gatagggagt aaactcttca tacgttctct
4621 atcactgata gggagtaaac tcgaggtgat aattccacgg ggttggggtt gcgccttttc
4681 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
4741 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
4801 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
4861 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
4921 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
4981 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
5041 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
5101 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
5161 tctccccagg gggatcatcg aattaccatg tctagactgg acaagagcaa agtcataaac
5221 tctgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
5281 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
5341 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccactcctg ccccctggaa
5401 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
5461 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
5521 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
5581 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
5641 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaaa
5701 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
5761 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
5821 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
5881 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactaagt
5941 aagatcctgt taatcaacct ctggattaca aaatttgtga aagattgact gatattctta
6001 actatgttgc tccttttacg ctgtgtggat atgctgcttt aatgcctctg tatcatgcta
6061 ttgcttcccg tacggctttc gttttctcct ccttgtataa atcctggttg ctgtctcttt
6121 atgaggagtt gtggcccgtt gtccgtcaac gtggcgtggt gtgctctgtg tttgctgacg
6181 caacccccac tggctggggc attgccacca cctgtcaact cctttctggg actttcgctt
6241 tccccctccc gatcgccacg gcagaactca tcgccgcctg ccttgcccgc tgctggacag
6301 gggctaggtt gctgggcact gataattccg tggtgttgtc ggggaagctg acgtcgattc
6361 ctgcggagat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
6421 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
6481 gaatgaaaga ccccacctgt aggtttggca agctagcttc tcgcttctgt tcgcgcgctt
6541 ctgctcccag acatcaacaa aagagcccac aacccctcac tcggggcgcc agtcctccga
6601 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
6661 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
6721 gtctttcacg cgtatgccgc atagttaagc caccgcggtg gcggtctgga ccacgccgga
6781 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
6841 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
6901 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
6961 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
7021 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
7081 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
7141 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atggctccga
7201 ccgaagccga cccgggcggc cccgccgacc ccgcacccgc ccccgaggcc caccgactct
7261 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
7321 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
7381 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
7441 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatggcg
7501 cgtcgaatta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
7561 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
7621 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
7681 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
7741 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
7801 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
7861 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgat gacgaaaggg
7921 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
7981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
8041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
8101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
8161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
8221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
8281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
8341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
8401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
8461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
8521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
8581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
8641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
8701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
8761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
8821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
8881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
8941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
9001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
9061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
9121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
9181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
9241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
9301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
9361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
9421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
9481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
9541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
9601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
9661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
9721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
9781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
9841 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
9901 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
9961 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
10021 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
10081 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
10141 cgcc
//
