出品公司: | 爱迪基因 |
---|---|
载体名称: | pLL3.7 |
质粒类型: | 慢病毒包装载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | mouse U6 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7650 bp |
5' 测序引物及序列: | U6 : 5'-cagtgcaggggaaagaatagtagac-3' |
3' 测序引物及序列: | -- |
载体标签: | GFP |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型: | -- |
病毒/非病毒: | 慢病毒 |
买家导航
pLL3.7载体质粒基本信息
pLL3.7质粒图谱载体图谱和pLL3.7载体序列质粒序列多克隆位点信息
pLL3.7质粒载体简介
pLL3.7质粒序列载体序列
LOCUS pLL3.7 7650 bp DNA SYN DEFINITION pLL3.7 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7650 /organism="pLL3.7" /mol_type="other DNA" promoter 239..815 /label="CMV_immearly_promoter" misc_feature 318..605 /label="CAG_enhancer" misc_feature 772..792 /label="CMV_fwd_primer" misc_feature 835..1015 /label="truncHIV-1_3_LTR" misc_feature 835..1015 /label="HIV-1_5_LTR" misc_feature 1126..1170 /label="HIV-1_psi_pack" CDS 1564..2451 /label="ORF frame 1" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 1686..1919 /label="RRE" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 2450..2465 /label="cPPT" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 2951..2967 /label="pBluescriptKS_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 3010..3043 /label="loxP" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" promoter 3108..3660 /label="CMV_immearly_promoter" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 3163..3450 /label="CAG_enhancer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" misc_feature 3617..3637 /label="CMV_fwd_primer" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" promoter 3618..3687 /label="CMV_promoter" /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN GSALRAPILQTNGSIHPQF*" CDS complement(3696..4466) /label="ORF frame 2" /translation="MLYEVIRSLDEFLLVQLVHAESDPGGGHELQQDHVIALLVGVFA QGGLGAQVVVVGQQHGAVADGGVLLVVVGELHAAVLDVVADLEVHLDAVLLLVGHDID VVAVVVVLQLVPQDVAVLLEVDALQLDAVHQGVALELHLGAGLVVAVVLEEDGALLDV AFGHGGLEEVVLLHVVGVAAEALHAVGQGGHEGGPGHGQLAGGADELQGQLAVGGIAL ALAGHAELVAVYVAVQLDQDGHHPGEQLLALAHHGGDR*" CDS 3711..4430 /label="ORF frame 3" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" gene 3714..4427 /label="EGFP" /gene="EGFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature complement(3756..3777) /label="EGFP_N_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 3894..3923 /label="Y66 (EGFP)" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 4364..4385 /label="EGFP_C_primer" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 4450..4483 /label="loxP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" misc_feature 4541..5128 /label="WPRE" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYK*" CDS 4629..5183 /label="ORF frame 3" /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS LWAASPHRYRRPRSRPRKTWSNHK*" CDS 4642..5682 /label="ORF frame 1" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" misc_feature complement(5131..5147) /label="pBluescriptKS_primer" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" misc_feature 5319..5334 /label="cPPT" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" misc_feature 5319..5340 /label="U3PPT" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" misc_feature 5656..5836 /label="HIV-1_5_LTR" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" misc_feature 5656..5836 /label="truncHIV-1_3_LTR" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" gene complement(6657..7517) /label="Ampicillin" /gene="Ampicillin" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTVDLDRDLEKHGAITSSNTAATNADCAWLEAQEEEEVGFPVTPQVPLRPMTYKA AVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGIRYPLTFGW CYKLVPVEQEKVEEANEGENTRLLHPVSLHGMDDPEREVLEWRFDSRLAFHHMARELH PDCTGSLWLDQI*" CDS complement(6657..7517) /label="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(7559..7587) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 2581 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg ccgccatctc 2641 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc 2701 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac 2761 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa 2821 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact 2881 gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgttaacgc 2941 gcggtgaccc tcgaggtcga cggtatcgat aagctcgctt cacgagattc cagcaggtcg 3001 agggacctaa taacttcgta tagcatacat tatacgaagt tatattaagg gttccaagct 3061 taagcggccg cgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt 3121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat 3181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 3241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 3301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 3361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 3421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag 3481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt 3541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac 3601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc 3661 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc atggtgagca 3721 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa 3781 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga 3841 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca 3901 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact 3961 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg 4021 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca 4081 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt 4141 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg 4201 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc 4261 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca 4321 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt 4381 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtag gaattcgtcg 4441 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc 4501 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa 4561 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 4621 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4681 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4741 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 4801 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 4861 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 4921 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc 4981 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 5041 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc 5101 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa 5161 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta 5221 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca 5281 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 5341 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa 5401 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc 5461 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa 5521 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga 5581 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg 5641 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 5701 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 5761 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 5821 tggaaaatct ctagcagcat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 5881 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 5941 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 6001 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 6061 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 6121 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 6181 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 6241 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6301 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 6361 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6421 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 6481 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6541 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6601 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6661 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6721 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6781 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6841 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6901 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6961 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 7021 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 7081 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 7141 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 7201 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 7261 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 7321 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 7381 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 7441 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 7501 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7561 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7621 cgcacatttc cccgaaaagt gccacctgac //
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