| 出品公司: | Agilent |
|---|---|
| 载体名称: | pB-CMV-MCS-EF1a-Puro |
| 质粒类型: | 哺乳动物细胞载体；ORF |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶 |
| 启动子: | CMV |
| 载体大小: | 6.1kb |
| 载体抗性: | Amp |
| 筛选标记: | Puro |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）用途: | 蛋白表达,转座 |
| 温度: | 37度 |
| 产品目录号: | #P24628 |
买家导航
pB-CMV-MCS-EF1a-Puro载体质粒基本信息
pB-CMV-MCS-EF1a-Puro质粒图谱载体图谱和pB-CMV-MCS-EF1a-Puro载体序列质粒序列多克隆位点信息
pB-CMV-MCS-EF1a-Puro质粒载体简介
pB-CMV-MCS-EF1a-Puro质粒序列载体序列
LOCUS pB-CMV-MCS-EF1a-Puro 6142 bp DNA circular SYN 24-JUN-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6142)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 24, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6142
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(1..105)
/gene="bla"
/label=AmpR promoter
rep_origin complement(131..586)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 728..744
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1442..1645
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1785..1996
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2006..2605
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 2713..2834
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4121..4137)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4145..4161
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4169..4199)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4214..4235
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4523..5111)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5282..6142)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
901 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
1081 aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc aaagccccca
1141 gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct
1201 ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg
1261 gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag
1321 acacctgggg ggatacgggg aaaaggcctc caaggcctac tagtattatg cccagtacat
1381 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
1441 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
1501 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
1561 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
1621 gtgggaggtt tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
1681 tccacgctgt tttgacctcc atagaagatt ctagagctag cgaattcgaa tttaaatcgg
1741 atccgcggcc gcaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc
1801 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag
1861 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
1921 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
1981 tgccgccaga acacagctga agcttatgac cgagtacaag cccacggtgc gcctcgccac
2041 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
2101 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
2161 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
2221 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
2281 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
2341 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
2401 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
2461 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
2521 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
2581 catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt
2641 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
2701 tttgtatcag ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
2761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
2821 atcaatgtat cttatcatgt ctggaattga ctcaaatgat gtcaattagt ctatcagaag
2881 ctatctggtc tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt
2941 cccaaactgg gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct
3001 cacaggaacg aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg
3061 attccttttt tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag
3121 gctcaaagag cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc
3181 cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga
3241 gccccgggcg gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg
3301 ctttgggggg gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc
3361 acgtaagtag aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac
3421 gtaaaagata atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac
3481 acttaccgca ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa
3541 atgcacagcg acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc
3601 gtcaatttta cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc
3661 gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa
3721 gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac
3781 tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg
3841 gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg
3901 cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat
3961 accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg
4021 ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata
4081 ccccgtgagt tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
4141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
4201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
4261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
4321 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
4381 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
4441 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
4501 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
4561 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
4621 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
4681 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
4741 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
4801 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4861 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4921 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
4981 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
5041 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
5101 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
5161 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
5221 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
5281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
5341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
5401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
5461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
5521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
5581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
5641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
5701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
5761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
5821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
5881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
5941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
6001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
6061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
6121 cacggaaatg ttgaatactc at
//
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