出品公司: | Agilent |
---|---|
载体名称: | pMUTIN4-GFP |
质粒类型: | ORF |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
载体大小: | 6.4kb |
载体抗性: | Amp |
克隆菌株: | DH5 alpha; 等 |
产品目录号: | P0913 |
质粒宿主: | 枯草杆菌 |
质粒用途: | 蛋白表达 |
买家导航
pMUTIN4-GFP载体质粒基本信息
pMUTIN4-GFP质粒图谱载体图谱和pMUTIN4-GFP载体序列质粒序列多克隆位点信息
pMUTIN4-GFP质粒载体简介
pMUTIN4-GFP质粒序列载体序列
LOCUS pMUTIN4-GFP 6416 bp DNA circular SYN 18-JUN-2024 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6416) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 18, 2024 from SnapGene 6.0.2 https://www.biofeng.com FEATURES Location/Qualifiers source 1..6416 /mol_type="other DNA" /organism="synthetic DNA construct" ORIGIN 1 aagaacgttg ctctagaata attctacaca gcccagtcca gactattcgg cactgaaatt 61 atgggaattc tacacagccc agtccagact attcggcact gaaattatgg gtgaagtggt 121 caagacctca ctaggcacct taaaaatagc gcaccctgaa gaagatttat ttgaggtagc 181 ccttgcctac ctagcttcca agaaagatat cctaacagca caagagcgga aagatgtttt 241 gttctacatc cagaacaacc tctgctaaaa ttcctgaaaa attttgcaaa aagttgttga 301 ctttatctac aaggtgtggc ataatgtgtg gaattgtgag cggataacaa ttaagcttaa 361 ggaggtgatc tagagtcgag ggtaccagcg ctatcgatcg gccggaagga gatatacata 421 tggctagcaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 481 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gctacatacg 541 gaaagcttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 601 ttgtcactac tttgacctat ggtgttcaat gcttttcccg ttatccggat catatgaaac 661 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt 721 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg 781 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca 841 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg 901 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag 961 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 1021 acctgtcgac acaatctgcc ctttcgaaag atcccaacga aaagcgtgac cacatggtcc 1081 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgagccc tacaaataat 1141 gaattcgagc actagtgcag cccgcctaat gagcgggctt ttttccatgc aagctaattc 1201 ggtggaaacg aggtcatcat ttccttccga aaaaacggtt gcatttaaat cttacatatg 1261 taatactttc aaagactaca tttgtaagat ttgatgtttg agtcggctga aagatcgtac 1321 gtaccaatta ttgtttcgtg attgttcaag ccataacact gtagggatag tggaaagagt 1381 gcttcatctg gttacgatca atcaaatatt caaacggagg gagacgattt tgatgaaacc 1441 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 1501 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aagtggaagc ggcgatggcg 1561 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 1621 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 1681 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1741 gtcgaagcct gtaaaacggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1801 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1861 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1921 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1981 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 2041 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 2101 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 2161 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 2221 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 2281 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 2341 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 2401 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2461 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2521 cgcaattaat gtgagttagg atcctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 2581 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 2641 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 2701 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 2761 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 2821 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 2881 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 2941 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 3001 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 3061 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 3121 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 3181 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 3241 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 3301 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 3361 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 3421 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 3481 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 3541 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 3601 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 3661 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 3721 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 3781 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 3841 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3901 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3961 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 4021 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 4081 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 4141 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 4201 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 4261 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 4321 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 4381 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 4441 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 4501 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 4561 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 4621 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 4681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 4741 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 4801 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt gatcctctag cacaaaaaga 4861 aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata agacggttcg 4921 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt 4981 aaaagaagtt atggaaataa gacttagaag caaacttaag agtgtgttga tagtgcagta 5041 tcttaaaatt ttgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa 5101 gaaggagtga ttacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa 5161 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa 5221 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt 5281 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc 5341 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata 5401 aaattgttgg gagtattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg 5461 aaagccatgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg 5521 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta 5581 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta 5641 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa 5701 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga 5761 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta 5821 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga 5881 aagttacacg ttactaaagg gaatgtagat aaattattag gtatactact gacagcttcc 5941 aaggagctaa agaggtccct agactctaga attcggatcc cgggaggaaa taattctatg 6001 agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta 6061 ggtatactac tgacagcttc caaggagcta aagaggtccc tagactctag acccggggat 6121 ctctgcagtc ggatctggta atgactctct agcttgaggc atcaaataaa acgaaaggct 6181 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt 6241 aggacaaatc cgccgctcta gctaagcaga aggccatcct gacggatggc ctttttgcgt 6301 ttctacaaac tcttgttaac tctagagctg cctgccgcgt ttcggtgatg aagatccgat 6361 gattaattaa ttcagaacgc tcggttgccg ccgggcgttt tttatgcagc aatggc //
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pMUTIN4-GFP质粒载体应用举例
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