| 质粒名称: | pET42b-ABE7.10 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 120398 |
| 存储实验室: | Junjiu Huang |
|
载体骨架基本信息 |
|
| 载体名称: | pET42b |
| 空载体大小: | 5248 bp |
| 完整质粒大小: | 10507 bp |
| 载体类型: | 细菌细胞表达 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ABE7.10/His6-TadA-SGGSSGGS-XTEN-SGGSSGGS-TadA*-SGGSSGGS-XTEN-SGGSSGGS-nCas9-SGGS-NLS |
| 物种来源: | Synthetic |
| 插入大小: | 5322bp |
| 启动子: | T7 |
| 标签/融合蛋白: | His (N terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | 未知 |
| 3’克隆位点: | 未知 |
| 5’测序引物 | TAATACGACTCACTATAGGG |
| 3’测序引物 | GCTAGTTATTGCTCAGCGG |
|
文献引用方法 |
|
| 材料和方法部分: | pET42b-ABE7.10 was a gift from Junjiu Huang (Addgene plasmid # 120398 ; http://n2t.net/addgene:120398 ; RRID:Addgene_120398) |
| 参考文献部分: | Genome-wide profiling of adenine base editor specificity by EndoV-seq. Liang P, Xie X, Zhi S, Sun H, Zhang X, Chen Y, Chen Y, Xiong Y, Ma W, Liu D, Huang J, Songyang Z. Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z. 10.1038/s41467-018-07988-z PubMed 30622278 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pET42b-ABE7.10 10570 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses ABE7.10-NLS with an N-terminal His Tag (His6) for
bacterial expression.
ACCESSION .
VERSION .
KEYWORDS pET42b-ABE7.10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10570)
AUTHORS Liang P, Xie X, Zhi S, Sun H, Zhang X, Chen Y, Chen Y, Xiong Y, Ma
W, Liu D, Huang J, Songyang Z
TITLE Genome-wide profiling of adenine base editor specificity by
EndoV-seq.
JOURNAL Nat Commun. 2019 Jan 8;10(1):67. doi: 10.1038/s41467-018-07988-z.
PUBMED 30622278
REFERENCE 2 (bases 1 to 10570)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10570
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 61..80
/label=T7
/note="T7 promoter, forward primer"
promoter 61..79
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 80..104
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 119..141
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 160..177
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 1366..5466
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5479..5499
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(5565..5583)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 5579..5626
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 5663..6118
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5750..5769)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5960..5981
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(6210..7025)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 6933..6952
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 7017..7036
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 7147..7735
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7636..7655
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7889..7906
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 7921..8060
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(7986..8005)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8146..8168
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(8162..8353)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
YELYRSCLARFGDDGENL"
CDS complement(9162..10244)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNHASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQLLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESWQ"
primer_bind 10206..10225
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(10245..10322)
/gene="lacI"
/label=lacI promoter
primer_bind 10528..10547
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
121 tgtttaactt taagaaggag atataccatg ggcagcagtc atcatcatca ccatcactcg
181 gaggttgaat tctcccacga gtattggatg cggcacgctc ttacgttagc aaaacgcgcg
241 tgggacgagc gtgaagtacc ggtaggcgcc gtgttagtgc ataataaccg ggtcattggt
301 gaaggatgga atcggccgat cgggagacac gatccgacag cacatgctga gattatggct
361 ttacggcaag gaggactggt tatgcagaac taccggttga ttgatgctac actgtacgta
421 accttagaac catgtgtgat gtgtgctgga gccatgatac attcccgcat cggaagagtg
481 gtttttgggg ctcgtgatgc aaaaactggc gccgccggaa gtcttatgga cgtgttacat
541 catccaggca tgaatcatcg ggtcgagatt acagagggca ttttggcaga tgaatgtgct
601 gcattgctta gtgatttctt ccgcatgcgg agacaggaaa tcaaagccca aaaaaaagct
661 caaagtagta ctgatagtgg tggatccagt ggaggctcgt caggctctga aacgcctggc
721 acatcagaat cggcaacgcc agagtcgtca ggaggttcct caggtggatc ttcggaggtc
781 gagttttcac atgagtattg gatgcgtcat gccttgacgt tggcgaaacg ggcgcgcgat
841 gagcgtgagg tgcccgtggg agcggtgttg gtactgaata accgggttat aggggaagga
901 tggaaccggg ctattgggtt acacgaccca acggcgcacg ccgagataat ggcactgcgc
961 caagggggct tagttatgca gaattatcgc cttatcgatg ctacactgta tgtaaccttt
1021 gaaccctgcg taatgtgtgc gggggctatg atccactcga gaatagggcg cgtggtattc
1081 ggcgtacgca acgctaaaac cggggctgcg ggctcgttga tggatgttct gcactacccc
1141 ggaatgaatc acagagtaga gatcacggag ggaattttgg ccgacgaatg tgcagcttta
1201 ctgtgctact tttttcggat gccgcggcaa gtcttcaacg cacagaagaa ggctcaatct
1261 tccactgact caggtggctc gagtggtggg agtagcggat ctgagacgcc aggcacatca
1321 gagagtgcaa cccccgagtc atcgggtggg agttccggcg gatctgataa gaaatactca
1381 ataggcttag ctatcggcac aaatagcgtc ggatgggcgg tgatcactga tgaatataag
1441 gttccgtcta aaaagttcaa ggttctggga aatacagacc gccacagtat caaaaaaaat
1501 cttatagggg ctcttttatt tgacagtgga gagacagcgg aagcgactcg tctcaaacgg
1561 acagctcgta gaaggtatac acgtcggaag aatcgtattt gttatctaca ggagattttt
1621 tcaaatgaga tggcgaaagt agatgatagt ttctttcatc gacttgaaga gtcttttttg
1681 gtggaagaag acaagaagca tgaacgtcat cctatttttg gaaatatagt agatgaagtt
1741 gcttatcatg agaaatatcc aactatctat catctgcgaa aaaaattggt agattctact
1801 gataaagcgg atttgcgctt aatctatttg gccttagcgc atatgattaa gtttcgtggt
1861 cattttttga ttgagggaga tttaaatcct gataatagtg atgtggacaa actatttatc
1921 cagttggtac aaacctacaa tcaattattt gaagaaaacc ctattaacgc aagtggagta
1981 gatgctaaag cgattctttc tgcacgattg agtaaatcaa gacgattaga aaatctcatt
2041 gctcagctcc ccggtgagaa gaaaaatggc ttatttggga atctcattgc tttgtcattg
2101 ggtttgaccc ctaattttaa atcaaatttt gatttggcag aagatgctaa attacagctt
2161 tcaaaagata cttacgatga tgatttagat aatttattgg cgcaaattgg agatcaatat
2221 gctgatttgt ttttggcagc taagaattta tcagatgcta ttttactttc agatatccta
2281 agagtaaata ctgaaataac taaggctccc ctatcagctt caatgattaa acgctacgat
2341 gaacatcatc aagacttgac tcttttaaaa gctttagttc gacaacaact tccagaaaag
2401 tataaagaaa tcttttttga tcaatcaaaa aacggatatg caggttatat tgatggggga
2461 gctagccaag aagaatttta taaatttatc aaaccaattt tagaaaaaat ggatggtact
2521 gaggaattat tggtgaaact aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac
2581 aacggctcta ttccccatca aattcacttg ggtgagctgc atgctatttt gagaagacaa
2641 gaagactttt atccattttt aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt
2701 cgaattcctt attatgttgg tccattggcg cgtggcaata gtcgttttgc atggatgact
2761 cggaagtctg aagaaacaat taccccatgg aattttgaag aagttgtcga taaaggtgct
2821 tcagctcaat catttattga acgcatgaca aactttgata aaaatcttcc aaatgaaaaa
2881 gtactaccaa aacatagttt gctttatgag tattttacgg tttataacga attgacaaag
2941 gtcaaatatg ttactgaagg aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa
3001 gccattgttg atttactctt caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa
3061 gattatttca aaaaaataga atgttttgat agtgttgaaa tttcaggagt tgaagataga
3121 tttaatgctt cattaggtac ctaccatgat ttgctaaaaa ttattaaaga taaagatttt
3181 ttggataatg aagaaaatga agatatctta gaggatattg ttttaacatt gaccttattt
3241 gaagataggg agatgattga ggaaagactt aaaacatatg ctcacctctt tgatgataag
3301 gtgatgaaac agcttaaacg tcgccgttat actggttggg gacgtttgtc tcgaaaattg
3361 attaatggta ttagggataa gcaatctggc aaaacaatat tagatttttt gaaatcagat
3421 ggttttgcca atcgcaattt tatgcagctg atccatgatg atagtttgac atttaaagaa
3481 gacattcaaa aagcacaagt gtctggacaa ggcgatagtt tacatgaaca tattgcaaat
3541 ttagctggta gccctgctat taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa
3601 ttggtcaaag taatggggcg gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa
3661 aatcagacaa ctcaaaaggg ccagaaaaat tcgcgagagc gtatgaaacg aatcgaagaa
3721 ggtatcaaag aattaggaag tcagattctt aaagagcatc ctgttgaaaa tactcaattg
3781 caaaatgaaa agctctatct ctattatctc caaaatggaa gagacatgta tgtggaccaa
3841 gaattagata ttaatcgttt aagtgattat gatgtcgatc acattgttcc acaaagtttc
3901 cttaaagacg attcaataga caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa
3961 tcggataacg ttccaagtga agaagtagtc aaaaagatga aaaactattg gagacaactt
4021 ctaaacgcca agttaatcac tcaacgtaag tttgataatt taacgaaagc tgaacgtgga
4081 ggtttgagtg aacttgataa agctggtttt atcaaacgcc aattggttga aactcgccaa
4141 atcactaagc atgtggcaca aattttggat agtcgcatga atactaaata cgatgaaaat
4201 gataaactta ttcgagaggt taaagtgatt accttaaaat ctaaattagt ttctgacttc
4261 cgaaaagatt tccaattcta taaagtacgt gagattaaca attaccatca tgcccatgat
4321 gcgtatctaa atgccgtcgt tggaactgct ttgattaaga aatatccaaa acttgaatcg
4381 gagtttgtct atggtgatta taaagtttat gatgttcgta aaatgattgc taagtctgag
4441 caagaaatag gcaaagcaac cgcaaaatat ttcttttact ctaatatcat gaacttcttc
4501 aaaacagaaa ttacacttgc aaatggagag attcgcaaac gccctctaat cgaaactaat
4561 ggggaaactg gagaaattgt ctgggataaa gggcgagatt ttgccacagt gcgcaaagta
4621 ttgtccatgc cccaagtcaa tattgtcaag aaaacagaag tacagacagg cggattctcc
4681 aaggagtcaa ttttaccaaa aagaaattcg gacaagctta ttgctcgtaa aaaagactgg
4741 gatccaaaaa aatatggtgg ttttgatagt ccaacggtag cttattcagt cctagttgtt
4801 gctaaggtgg aaaaagggaa atcgaagaag ttaaaatccg ttaaagagtt actagggatc
4861 acaattatgg aaagaagttc ctttgaaaaa aatccgattg actttttaga agctaaagga
4921 tataaggaag ttaaaaaaga cttaatcatt aaactaccta aatatagtct ttttgagtta
4981 gaaaacggtc gtaaacggat gctggctagt gccggagaat tacaaaaagg aaatgagctg
5041 gctctgccaa gcaaatatgt gaatttttta tatttagcta gtcattatga aaagttgaag
5101 ggtagtccag aagataacga acaaaaacaa ttgtttgtgg agcagcataa gcattattta
5161 gatgagatta ttgagcaaat cagtgaattt tctaagcgtg ttattttagc agatgccaat
5221 ttagataaag ttcttagtgc atataacaaa catagagaca aaccaatacg tgaacaagca
5281 gaaaatatta ttcatttatt tacgttgacg aatcttggag ctcccgctgc ttttaaatat
5341 tttgatacaa caattgatcg taaacgatat acgtctacaa aagaagtttt agatgccact
5401 cttatccatc aatccatcac tggtctttat gaaacacgca ttgatttgag tcagctagga
5461 ggtgactctg gcgggtctcc caagaagaag aggaaagtct aataattgat taatacctag
5521 gctgctaaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
5581 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
5641 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
5701 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
5761 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
5821 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
5881 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
5941 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccatatc
6001 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
6061 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
6121 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
6181 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
6241 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
6301 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
6361 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
6421 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
6481 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
6541 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
6601 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
6661 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
6721 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
6781 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
6841 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
6901 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
6961 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
7021 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
7141 atcttcttga gatccttttt ttctgcgcgt aatctgctga ttgcaaacaa aaaaaccacc
7201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
7261 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
7321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatca tgttaccagt
7381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
7441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccaa gcttggagcg
7501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
7561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
7621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
7681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
7741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
7801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
7861 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
7921 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
7981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
8041 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
8101 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
8161 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcataag
8221 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
8281 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
8341 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
8401 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
8461 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
8521 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
8581 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
8641 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
8701 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
8761 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
8821 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
8881 tgacgaaggc ttgagcgagg gagtgcaaga ttccgaatac cgcaagcgac aggccgatca
8941 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
9001 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
9061 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
9121 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgcca gctttccagt
9181 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
9241 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg
9301 cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc
9361 aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta
9421 tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg
9481 cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc
9541 tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt
9601 tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga
9661 cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg
9721 accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg
9781 ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca
9841 gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg
9901 agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc
9961 accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc
10021 gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acaactgttt gcccgccagt
10081 tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc
10141 cgcgttttcg cagaaacgtg gctggcatgg ttcaccacgc gggaaacggt ctgataagag
10201 acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat
10261 tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg
10321 tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag
10381 gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa
10441 cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc
10501 aaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc
10561 acctgtggcg
//
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