| 质粒名称: | mTagBFP2-TOMM20-N-10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55328 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mTagBFP2-N1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TOMM20,NM_014765.2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | mTagBFP2（主干上的C终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mTagBFP2-TOMM20-N-10 was a gift from Michael Davidson (Addgene plasmid # 55328 ; http://n2t.net/addgene:55328 ; RRID:Addgene_55328) |
| 参考文献部分: | An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV. PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8. 10.1371/journal.pone.0028674 PubMed 22174863 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mTagBFP2-TOMM20-N-10 5115 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: Outer Mitochondrial Membrane, Excitation: 399,
Emission: 456.
ACCESSION .
VERSION .
KEYWORDS mTagBFP2-TOMM20-N-10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5115)
AUTHORS Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
TITLE An enhanced monomeric blue fluorescent protein with the high
chemical stability of the chromophore.
JOURNAL PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
PUBMED 22174863
REFERENCE 2 (bases 1 to 5115)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5115
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 73..867
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(127..146)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 737..756
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(1055..1074)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 1099..1146
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 1475..2063
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1964..1983
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 2238..2541
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2542..2745
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2696..2716
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 2774..2783
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 3239..3248
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3245..3955
/codon_start=1
/product="enhanced monomeric blue fluorescent protein
(Subach et al., 2011)"
/label=mTagBFP2
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
ARYCDLPSKLGHKLN"
polyA_signal 4080..4201
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4117..4136)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4171..4190
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(4208..4663)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4345..4366)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4557..4576
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 4690..4794
/gene="bla"
/label=AmpR promoter
promoter 4796..38
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(4819..4839)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 5004..24
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5066..5085
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
ORIGIN
1 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
61 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
121 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
181 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
241 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
301 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
361 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
421 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
481 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
541 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
601 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
661 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
721 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
781 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
841 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
901 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
961 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
1021 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
1081 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
1141 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
1201 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
1261 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
1321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
1381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
2041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
2161 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
2221 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
2281 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
2341 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
2401 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
2461 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
2521 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
2581 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
2641 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
2701 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
2761 cagatccgct agcgccacca tggtgggtcg gaacagcgcc atcgccgccg gtgtatgcgg
2821 ggcccttttc attgggtact gcatctactt cgaccgcaaa agacgaagtg accccaactt
2881 caagaacagg cttcgagaac gaagaaagaa acagaagctt gccaaggaga gagctgggct
2941 ttccaagtta cctgacctta aagatgctga agctgttcag aagttcttcc ttgaagaaat
3001 acagcttggt gaagagttac tagctcaagg tgaatatgag aagggcgtag accatctgac
3061 aaatgcaatt gctgtgtgtg gacagccaca gcagttactg caggtcttac agcaaactct
3121 tccaccacca gtgttccaga tgcttctgac taagctccca acaattagtc agagaattgt
3181 aagtgctcag agcttggctg aagatgatgt ggaaggcggt agcggggatc caccggtcgc
3241 caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
3301 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
3361 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
3421 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
3481 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
3541 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
3601 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
3661 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
3721 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa
3781 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
3841 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
3901 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaattaagc
3961 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
4021 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
4081 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
4141 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
4201 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
4261 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
4321 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
4381 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
4441 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
4501 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
4561 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
4621 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
4681 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
4741 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
4801 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
4861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
4921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
4981 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
5041 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
5101 agctattcca gaagt
//
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