| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.84 ; pGL4.84[hRlucCP/Puro] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；海肾荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4866 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | hRlucCP |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.84[hRlucCP/Puro]载体是信号通路报告载体；无启动子；含海肾荧光素酶报告基因hRlucCP。 |
| 产品目录号: | E7521 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.84[hRlucCP/Puro]载体pGL4.84质粒基本信息
pGL4.84[hRlucCP/Puro]质粒图谱pGL4.84载体图谱和pGL4.84[hRlucCP/Puro]载体序列pGL4.84质粒序列多克隆位点信息
pGL4.84[hRlucCP/Puro]质粒pGL4.84载体简介
pGL4.84载体简介 The pGL4.84[hRlucCP/Puro] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. It has also been engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.84[hRlucCP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
pGL4.84[hRlucCP/Puro]质粒序列pGL4.84载体序列
LOCUS DQ188848 4866 bp DNA circular SYN 16-NOV-2005
DEFINITION Synthetic Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro],
complete sequence.
ACCESSION DQ188848
VERSION DQ188848.1 GI:76364322
KEYWORDS .
SOURCE Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro]
ORGANISM Renilla luciferase reporter vector pGL4.84[hRlucCP/Puro]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4866)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4866)
AUTHORS Stecha,P., Paguio,A. and Chauvin,F.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2005) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..4866
/organism="Renilla luciferase reporter vector
pGL4.84[hRlucCP/Puro]"
/mol_type="other DNA"
/db_xref="taxon:346095"
misc_feature 1..70
/note="multiple cloning region"
CDS 100..1212
/note="synthetic Renilla luciferase; contains hPEST and
hCL1 destabilization sequences"
/codon_start=1
/product="hRlucCP"
/protein_id="ABA41686.1"
/db_xref="GI:76364323"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSACKNWFSSLSHFVIHLNSHGF
PPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
regulatory 1249..1470
/regulatory_class="other"
/note="SV40 late poly(A) signal"
regulatory 1518..1936
/regulatory_class="promoter"
/note="SV40 early enhancer/promoter"
CDS 1961..2560
/note="puromycin resistance"
/codon_start=1
/product="puromycin-N-acetyltransferase"
/protein_id="ABA41687.1"
/db_xref="GI:76364324"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
regulatory 2585..2633
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal"
primer_bind 2700..2719
/note="Reporter Vector primer 4 (RVprimer4) binding
region"
rep_origin 2957
/note="ColEI-derived plasmid replication origin"
gene complement(3748..4608)
/gene="bla"
CDS complement(3748..4608)
/gene="bla"
/note="ampicillin resistance"
/codon_start=1
/product="beta-lactamase"
/protein_id="ABA41688.1"
/db_xref="GI:76364325"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4713..4866
/regulatory_class="polyA_signal_sequence"
/note="synthetic poly(A) signal/transcriptional pause
site"
primer_bind 4815..4834
/note="Reporter Vector primer 3 (RVprimer3) binding
region"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
1021 aagaacgagc agaattctgc ttgcaagaac tggttcagta gcttaagcca ctttgtgatc
1081 caccttaaca gccacggctt ccctcccgag gtggaggagc aggccgccgg caccctgccc
1141 atgagctgcg cccaggagag cggcatggat agacaccctg ctgcttgcgc cagcgccagg
1201 atcaacgtct aactgcagtc tagagtcggg gcggccggcc gcttcgagca gacatgataa
1261 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1321 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
1381 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
1441 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgttt gcgtattggg
1501 cgctcttccg ctgatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
1561 gttagctacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
1621 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
1681 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
1741 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
1801 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
1861 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
1921 cttttgcaaa aagctcgatt cttctgacac tagcgccacc atgaccgagt acaagcctac
1981 cgtgcgcctg gccactcgcg atgatgtgcc ccgcgccgtc cgcactctgg ccgccgcttt
2041 cgccgactac cccgctaccc ggcacaccgt ggaccccgac cggcacatcg agcgtgtgac
2101 agagttgcag gagctgttcc tgacccgcgt cgggctggac atcggcaagg tgtgggtagc
2161 cgacgacggc gcggccgtgg ccgtgtggac tacccccgag agcgttgagg ccggcgccgt
2221 gttcgccgag atcggccccc gaatggccga gctgagcggc agccgcctgg ccgcccagca
2281 gcaaatggag ggcctgcttg ccccccatcg tcccaaggag cctgcctggt ttctggccac
2341 tgtaggagtg agccccgacc accagggcaa gggcttgggc agcgccgtcg tgttgcccgg
2401 cgtagaggcc gccgaacgcg ccggtgtgcc cgcctttctc gaaacaagcg caccaagaaa
2461 ccttccattc tacgagcgcc tgggcttcac cgtgaccgcc gatgtcgagg tgcccgaggg
2521 acctaggacc tggtgtatga cacgaaaacc tggcgcctaa tgatctagaa ccggtcatgg
2581 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac
2641 tagatgctgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
2701 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
2761 acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
2821 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
2881 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
2941 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
3001 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
3061 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
3121 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
3181 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
3241 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
3301 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
3361 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
3421 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
3481 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
3541 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
3601 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
3661 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagcg
3721 gccgcaaatg ctaaaccact gcagtggtta ccagtgcttg atcagtgagg caccgatctc
3781 agcgatctgc ctatttcgtt cgtccatagt ggcctgactc cccgtcgtgt agatcactac
3841 gattcgtgag ggcttaccat caggccccag cgcagcaatg atgccgcgag agccgcgttc
3901 accggccccc gatttgtcag caatgaacca gccagcaggg agggccgagc gaagaagtgg
3961 tcctgctact ttgtccgcct ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag
4021 aagttcgcca gtgagtagtt tccgaagagt tgtggccatt gctactggca tcgtggtatc
4081 acgctcgtcg ttcggtatgg cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac
4141 atgatcaccc atattatgaa gaaatgcagt cagctcctta gggcctccga tcgttgtcag
4201 aagtaagttg gccgcggtgt tgtcgctcat ggtaatggca gcactacaca attctcttac
4261 cgtcatgcca tccgtaagat gcttttccgt gaccggcgag tactcaacca agtcgttttg
4321 tgagtagtgt atacggcgac caagctgctc ttgcccggcg tctatacggg acaacaccgc
4381 gccacatagc agtactttga aagtgctcat catcgggaat cgttcttcgg ggcggaaaga
4441 ctcaaggatc ttgccgctat tgagatccag ttcgatatag cccactcttg cacccagttg
4501 atcttcagca tcttttactt tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa
4561 tgccgcaaag aagggaatga gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt
4621 tcaatattat tgaagcattt atcagggtta ctagtacgtc tctcaaggat aagtaagtaa
4681 tattaaggta cgggaggtat tggacaggcc gcaataaaat atctttattt tcattacatc
4741 tgtgtgttgg ttttttgtgt gaatcgatag tactaacata cgctctccat caaaacaaaa
4801 cgaaacaaaa caaactagca aaataggctg tccccagtgc aagtgcaggt gccagaacat
4861 ttctct
//
