质粒名称: | pGBKT7-GW |
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出品公司: | Addgene |
目录编号: | 61703 |
存储实验室: | Yuhai Cui |
载体骨架基本信息 |
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载体名称: | pGBKT7 |
载体出品公司: | Clontech |
载体抗性: | 氯霉素和卡那霉素 |
空载体大小: | 7300 bp |
完整质粒大小: | -- |
载体修饰: | 网关磁带被克隆到MCS中 |
载体类型: | 酵母表达 |
筛选标记: | TRP1 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | Gateway克隆 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pGBKT7-GW was a gift from Yuhai Cui (Addgene plasmid # 61703 ; http://n2t.net/addgene:61703 ; RRID:Addgene_61703) |
参考文献部分: | Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y. Plant J. 2010 Jan;61(2):259-70. doi: 10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16. 10.1111/j.1365-313X.2009.04048.x PubMed 19843313 |
买家导航
pGBKT7GW载体基本信息
pGBKT7GW载体图谱质粒图谱和多克隆位点信息
pGBKT7GW载体简介
pGBKT7GW载体序列
LOCUS pGBKT7-GW 8981 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Gateway compatible Y2H vector - BD. ACCESSION . VERSION . KEYWORDS pGBKT7-GW SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8981) AUTHORS Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y TITLE Arabidopsis homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. JOURNAL Plant J. 2010 Jan;61(2):259-70. doi: 10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16. PUBMED 19843313 REFERENCE 2 (bases 1 to 8981) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8981 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 30..734 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 762..1202 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="DNA binding domain of the GAL4 transcriptional activator" /label=GAL4 DNA binding domain /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS" primer_bind 1213..1232 /label=T7 /note="T7 promoter, forward primer" promoter 1213..1231 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1251..1280 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind 1287..1411 /gene="mutant version of attR" /label=attR1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" promoter 1436..1466 /label=lac UV5 promoter /note="E. coli lac promoter with an ""up"" mutation" CDS 1520..2179 /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind complement(1586..1605) /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" CDS 2521..2826 /codon_start=1 /gene="ccdB" /product="CcdB, a bacterial toxin that poisons DNA gyrase" /label=ccdB /note="Plasmids containing the ccdB gene cannot be propagated in standard E. coli strains." /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" primer_bind 2705..2724 /label=ccdB-fwd /note="ccdB gene, forward primer" protein_bind complement(2867..2991) /gene="mutant version of attR" /label=attR2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" terminator 3015..3062 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 3089..3276 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(3144..3168) /label=pHybLex-R /note="ADH terminator, reverse primer" primer_bind complement(3300..3316) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3300..3316) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3313..3335) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3324..3340 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3348..3378) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3393..3414 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3531..3548) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3702..4290) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3782..3801) /label=pBR322ori-F /note="pBR322 origin, forward primer" polyA_signal 4619..4666 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 4691..4710 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" CDS complement(4899..5693) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHDDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(5010..5029) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 5620..5639 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter complement(5694..5798) /gene="bla" /label=AmpR promoter rep_origin 5825..7167 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(6788..6835) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind 7208..7226 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(7264..7286) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 7386..7405 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 7426..7707 /gene="S. cerevisiae TRP1" /label=TRP1 promoter CDS 7708..8382 /codon_start=1 /gene="S. cerevisiae TRP1" /product="phosphoribosylanthranilate isomerase, required for tryptophan biosynthesis" /label=TRP1 /note="yeast auxotrophic marker" /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA KK" rep_origin complement(8485..8940) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(8622..8643) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 8834..8853 /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1261 tgatctcaga ggaggacctg catatgacaa gtttgtacaa aaaagctgaa cgagaaacgt 1321 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat 1381 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac 1441 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc 1501 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc 1561 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa 1621 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa 1681 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg 1741 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt 1801 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg 1861 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt 1921 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac 1981 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg 2041 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc 2101 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga 2161 gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat aacagtatgc 2221 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat 2281 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat 2341 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga 2401 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg 2461 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa 2521 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta 2581 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt 2641 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc 2701 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg 2761 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga 2821 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat 2881 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt 2941 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg tctcgacctg 3001 cagcggccgc ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt 3061 tgcgcgcttg cagccaagct aattccgggc gaatttctta tgatttatga tttttattat 3121 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa 3181 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata 3241 gcatgaggtc gctcttattg accacacctc taccggcatg caagcttggc gtaatcatgg 3301 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 3361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 3421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 3481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 3541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 3601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 3661 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 3721 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 3781 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 3841 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 3901 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 3961 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 4021 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 4081 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 4141 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 4201 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 4261 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 4321 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 4381 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 4441 gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag ccctgggcct 4501 tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta ttggccccaa 4561 tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga 4621 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat agcgcgggtt 4681 ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg ggcgaagaac 4741 tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg 4801 aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc 4861 gtcgcttggt cggttcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg 4921 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg 4981 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga 5041 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc 5101 accatgatat tcggcaagca ggcatcgtca tgggtcacga cgagatcctc gccgtcgggc 5161 atgctcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc 5221 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt 5281 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca 5341 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc 5401 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct 5461 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca 5521 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc 5581 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc 5641 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catactcttc 5701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 5761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 5821 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat 5881 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct 5941 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg 6001 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga 6061 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga 6121 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat 6181 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact 6241 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt 6301 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac 6361 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg 6421 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt 6481 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc 6541 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc 6601 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa 6661 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac 6721 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa 6781 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc 6841 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt 6901 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat 6961 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta 7021 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc 7081 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa 7141 tgctatcatt tcctttgata ttggatcata ttaagaaacc attattatca tgacattaac 7201 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 7261 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 7321 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 7381 ctatgcggca tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata 7441 tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta 7501 tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg 7561 tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa 7621 agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg 7681 attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt 7741 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc 7801 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt 7861 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa 7921 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat 7981 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc 8041 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt 8101 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca 8161 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa 8221 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga 8281 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac 8341 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat 8401 ttatttaagt attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc 8461 gtaaggagaa aataccgcat caggaaattg taaacgttaa tattttgtta aaattcgcgt 8521 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 8581 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 8641 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 8701 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 8761 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 8821 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 8881 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 8941 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga t //