出品公司: | Addgene |
---|---|
载体名称: | pX603 或 pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;哺乳动物细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | CMV |
载体大小: | 7101 bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | BGH Reverse:TAGAAGGCACAGTCGAGG |
载体标签: | 3xHA (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞(系): | 哺乳动物细胞 |
备注: | -- |
产品目录号: | #61594 |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pX603载体质粒基本信息
pX603质粒图谱载体图谱和pX603载体序列质粒序列多克隆位点信息
pX603质粒载体简介
pX603质粒序列载体序列
LOCUS pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA 7101 bp DNA SYN DEFINITION pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7101 /organism="pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA" /mol_type="other DNA" rep_origin complement(47..666) /label="pBR322_origin" gene complement(821..1681) /label="Ampicillin" /gene="Ampicillin" CDS complement(821..1681) /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(1723..1751) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature complement(1910..1932) /label="pGEX_3_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin complement(2200..2506) /label="f1_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter 2759..3335 /label="CMV_immearly_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 2838..3125 /label="CAG_enhancer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 3292..3312 /label="CMV_fwd_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" CDS 3297..6710 /label="ORF frame 3" /translation="MGGRRVRWEVYISRALWLTTGATMAPKKKRKVGIHGVPAAKRNY ILGLAIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEGRRSKRGARRLKRRRRHR IQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEFSAALLHLAKRRGVHNV NEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEVRGSINRFKTSDYVKE AKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGWKDIKEWYEMLMGHC TYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQIIENVFKQKKKPT LKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEIIENAELLDQIAK ILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAINLILDELWHTN DNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIKVINAIIKKYG LPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAKYLIEKIKLH DMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQEEASKKGN RTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFSVQKDFIN RNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERNKGYKHH AEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIFITPHQ IKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDNDKLK KLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKDNGP VIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNLDV IKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLNR IEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI IKKGKRPAATKKAGQAKKKKGSYPYDVPDYAYPYDVPDYAYPYDVPDYA*" CDS complement(3348..6083) /label="ORF frame 2" /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*" misc_feature 3372..3392 /label="SV40 NLS" /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*" misc_feature 3372..3395 /label="SV40 NLS" /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*" misc_feature 3378..3392 /label="NLS" /translation="MGVQFVAVILNLLDHGAVVLFGVLGQVVPGFLVVLVQGILLVAV LFHNQLQFLVGLGVVVVHQQLFGALVDQLFQLVIVLVVQAVQIVHDQGVALVVLPGGV QGVVNQLSIRLLVHPVAVLVVLEVLNVLDLVGGDEDLFVLLLGFDLGHALGLLFLEHL VFHHFFGLVQFLPLFEDEIGVGNDQGVLGVVLVPLVPLFLKLPLPPQKAGEAAIDGLH FHVQVVHSEVAPQQVHQASGGVSGIHQVPVDEVFLHGEPVDVPFFQQILFLGLADSAL ALGQIQDVLLEGFVADLAVAAAQVLEWGPVALLAGFFLLHEHLVVEAVVEGHASGDDV VHLIVEGVVQQIFQRDGFQAVQALAFLHVVQLDLLDQVLGVLFAGGPDDFLDPLVGLP VPLLHLVDHFLGVLGVLLAGQLDNDVVGQAVLLDDGVDHFDALDEASLHDGAQDEVVH QGGGDLFLLGQVHLLGHQLQPVEDSDLVVVGVPQLVQDQVDGLQAQVVGAGIALQIRD LLDLFLGQLGVQIGQFFLDVLAALVDGQDLGNLIQQLGVLNNLFPGGNVLDVVVHLQV GELGLAGAGHSVALNILFVHEDFFGDLLQGGLLLLLEHVLDDLELLVIFQLLVLVPGD HEIVQVVQGVVQVGVVGVLHAPQFLGEVGAVAHQHLVPFFDVLPAEGAALARSLIVGP PGFQQVDVGVDEALVQLVVGLLHLQQLFGFFHVVAGLESVDAAPHFAVFLQPFQLQFG HVFLFQGLAVPADLLFGGQLVAGVLFHLVHVVHASSLGQVQQGGRELFLAQLLAQALH SGLVGVDAAQLAVVGQQVVVEQQLLHSLDSMPPPPLQPSGASLAPPALVVFHVGLFEQ PHAGIDHVPCLVVDDAVAHAGDADGQAQDVVPLGCWDSVDTDLPLLLWGHGGTGS*" CDS complement(4577..5035) /label="ORF frame 3" /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*" misc_feature 6627..6653 /label="HA" /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*" misc_feature complement(6735..6752) /label="BGH_rev_primer" /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*" terminator 6738..6941 /label="bGH_PA_terminator" /translation="MASRLYRHLPSCMSCSLIFSIRYLAFSLPVVRMISSIRSLVCRF RFCISLIIFWASLEFFSRASSIMMSLGRPYFLMMALITLMLWMKLLFTTGLRMKSSTR VVGISFCWDRSTFLGTSFSRLKIAIWLSLVCHSSSRIRLMAFRLRLWVPV*" ORIGIN 1 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 61 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 121 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 181 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 241 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 301 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 361 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 421 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 481 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 541 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 601 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 661 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 721 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 781 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 841 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 901 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 961 ggcttccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 1021 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 1081 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 1141 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 1201 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 1261 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 1321 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 1381 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 1441 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 1501 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 1561 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 1621 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 1681 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 1741 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 1801 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 1861 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 1921 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 1981 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 2041 agcagattgt actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg 2101 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 2161 cttataaatc aaaagaatag cccgagatag ggttgagtgt tgttccagtt tggaacaaga 2221 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 2281 atggcccact acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag 2341 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 2401 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 2461 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 2521 cgtactatgg ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa 2581 ataccgcatc aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg 2641 ggcaaagccc gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg 2701 cagagaggga gtggccaact ccatcactag gggttcctgc ggccgcctcg aggcgttgac 2761 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 2821 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 2881 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 2941 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 3001 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 3061 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 3121 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 3181 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 3241 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 3301 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actaccggtg 3361 ccaccATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA GCAGCCAAGC 3421 GGAACTACAT CCTGGGCCTG GcCATCGGCA TCACCAGCGT GGGCTACGGC ATCATCGACT 3481 ACGAGACACG GGACGTGATC GATGCCGGCG TGCGGCTGTT CAAAGAGGCC AACGTGGAAA 3541 ACAACGAGGG CAGGCGGAGC AAGAGAGGCG CCAGAAGGCT GAAGCGGCGG AGGCGGCATA 3601 GAATCCAGAG AGTGAAGAAG CTGCTGTTCG ACTACAACCT GCTGACCGAC CACAGCGAGC 3661 TGAGCGGCAT CAACCCCTAC GAGGCCAGAG TGAAGGGCCT GAGCCAGAAG CTGAGCGAGG 3721 AAGAGTTCTC TGCCGCCCTG CTGCACCTGG CCAAGAGAAG AGGCGTGCAC AACGTGAACG 3781 AGGTGGAAGA GGACACCGGC AACGAGCTGT CCACCAAAGA GCAGATCAGC CGGAACAGCA 3841 AGGCCCTGGA AGAGAAATAC GTGGCCGAAC TGCAGCTGGA ACGGCTGAAG AAAGACGGCG 3901 AAGTGCGGGG CAGCATCAAC AGATTCAAGA CCAGCGACTA CGTGAAAGAA GCCAAACAGC 3961 TGCTGAAGGT GCAGAAGGCC TACCACCAGC TGGACCAGAG CTTCATCGAC ACCTACATCG 4021 ACCTGCTGGA AACCCGGCGG ACCTACTATG AGGGACCTGG CGAGGGCAGC CCCTTCGGCT 4081 GGAAGGACAT CAAAGAATGG TACGAGATGC TGATGGGCCA CTGCACCTAC TTCCCCGAGG 4141 AACTGCGGAG CGTGAAGTAC GCCTACAACG CCGACCTGTA CAACGCCCTG AACGACCTGA 4201 ACAATCTCGT GATCACCAGG GACGAGAACG AGAAGCTGGA ATATTACGAG AAGTTCCAGA 4261 TCATCGAGAA CGTGTTCAAG CAGAAGAAGA AGCCCACCCT GAAGCAGATC GCCAAAGAAA 4321 TCCTCGTGAA CGAAGAGGAT ATTAAGGGCT ACAGAGTGAC CAGCACCGGC AAGCCCGAGT 4381 TCACCAACCT GAAGGTGTAC CACGACATCA AGGACATTAC CGCCCGGAAA GAGATTATTG 4441 AGAACGCCGA GCTGCTGGAT CAGATTGCCA AGATCCTGAC CATCTACCAG AGCAGCGAGG 4501 ACATCCAGGA AGAACTGACC AATCTGAACT CCGAGCTGAC CCAGGAAGAG ATCGAGCAGA 4561 TCTCTAATCT GAAGGGCTAT ACCGGCACCC ACAACCTGAG CCTGAAGGCC ATCAACCTGA 4621 TCCTGGACGA GCTGTGGCAC ACCAACGACA ACCAGATCGC TATCTTCAAC CGGCTGAAGC 4681 TGGTGCCCAA GAAGGTGGAC CTGTCCCAGC AGAAAGAGAT CCCCACCACC CTGGTGGACG 4741 ACTTCATCCT GAGCCCCGTC GTGAAGAGAA GCTTCATCCA GAGCATCAAA GTGATCAACG 4801 CCATCATCAA GAAGTACGGC CTGCCCAACG ACATCATTAT CGAGCTGGCC CGCGAGAAGA 4861 ACTCCAAGGA CGCCCAGAAA ATGATCAACG AGATGCAGAA GCGGAACCGG CAGACCAACG 4921 AGCGGATCGA GGAAATCATC CGGACCACCG GCAAAGAGAA CGCCAAGTAC CTGATCGAGA 4981 AGATCAAGCT GCACGACATG CAGGAAGGCA AGTGCCTGTA CAGCCTGGAA GCCATCCCTC 5041 TGGAAGATCT GCTGAACAAC CCCTTCAACT ATGAGGTGGA CCACATCATC CCCAGAAGCG 5101 TGTCCTTCGA CAACAGCTTC AACAACAAGG TGCTCGTGAA GCAGGAAGAA gccagCAAGA 5161 AGGGCAACCG GACCCCATTC CAGTACCTGA GCAGCAGCGA CAGCAAGATC AGCTACGAAA 5221 CCTTCAAGAA GCACATCCTG AATCTGGCCA AGGGCAAGGG CAGAATCAGC AAGACCAAGA 5281 AAGAGTATCT GCTGGAAGAA CGGGACATCA ACAGGTTCTC CGTGCAGAAA GACTTCATCA 5341 ACCGGAACCT GGTGGATACC AGATACGCCA CCAGAGGCCT GATGAACCTG CTGCGGAGCT 5401 ACTTCAGAGT GAACAACCTG GACGTGAAAG TGAAGTCCAT CAATGGCGGC TTCACCAGCT 5461 TTCTGCGGCG GAAGTGGAAG TTTAAGAAAG AGCGGAACAA GGGGTACAAG CACCACGCCG 5521 AGGACGCCCT GATCATTGCC AACGCCGATT TCATCTTCAA AGAGTGGAAG AAACTGGACA 5581 AGGCCAAAAA AGTGATGGAA AACCAGATGT TCGAGGAAAA GCAGGCCGAG AGCATGCCCG 5641 AGATCGAAAC CGAGCAGGAG TACAAAGAGA TCTTCATCAC CCCCCACCAG ATCAAGCACA 5701 TTAAGGACTT CAAGGACTAC AAGTACAGCC ACCGGGTGGA CAAGAAGCCT AATAGAGAGC 5761 TGATTAACGA CACCCTGTAC TCCACCCGGA AGGACGACAA GGGCAACACC CTGATCGTGA 5821 ACAATCTGAA CGGCCTGTAC GACAAGGACA ATGACAAGCT GAAAAAGCTG ATCAACAAGA 5881 GCCCCGAAAA GCTGCTGATG TACCACCACG ACCCCCAGAC CTACCAGAAA CTGAAGCTGA 5941 TTATGGAACA GTACGGCGAC GAGAAGAATC CCCTGTACAA GTACTACGAG GAAACCGGGA 6001 ACTACCTGAC CAAGTACTCC AAAAAGGACA ACGGCCCCGT GATCAAGAAG ATTAAGTATT 6061 ACGGCAACAA ACTGAACGCC CATCTGGACA TCACCGACGA CTACCCCAAC AGCAGAAACA 6121 AGGTCGTGAA GCTGTCCCTG AAGCCCTACA GATTCGACGT GTACCTGGAC AATGGCGTGT 6181 ACAAGTTCGT GACCGTGAAG AATCTGGATG TGATCAAAAA AGAAAACTAC TACGAAGTGA 6241 ATAGCAAGTG CTATGAGGAA GCTAAGAAGC TGAAGAAGAT CAGCAACCAG GCCGAGTTTA 6301 TCGCCTCCTT CTACAACAAC GATCTGATCA AGATCAACGG CGAGCTGTAT AGAGTGATCG 6361 GCGTGAACAA CGACCTGCTG AACCGGATCG AAGTGAACAT GATCGACATC ACCTACCGCG 6421 AGTACCTGGA AAACATGAAC GACAAGAGGC CCCCCAGGAT CATTAAGACA ATCGCCTCCA 6481 AGACCCAGAG CATTAAGAAG TACAGCACAG ACATTCTGGG CAACCTGTAT GAAGTGAAAT 6541 CTAAGAAGCA CCCTCAGATC ATCAAAAAGG GCAAAAGGCC GGCGGCCACG AAAAAGGCCG 6601 GCCAGGCAAA AAAGAAAAAG ggatccTACC CATACGATGT TCCAGATTAC GCTTATCCCT 6661 ACGACGTGCC TGATTATGCA TACCCATATG ATGTCCCCGA CTATGCCTAA gaattcctag 6721 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc 6781 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga 6841 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca 6901 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggagC TAGAggccgc 6961 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 7021 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 7081 gagcgcgcag ctgcctgcag g //
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pX603质粒载体应用举例
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