| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141374 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 8825 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | NEB Stable |
| 物种来源: | SARS-CoV-2 |
| 插入大小: | 951 bp |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141374 ; http://n2t.net/addgene:141374 ; RRID:Addgene_141374)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro 9510 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9510)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9510)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9510
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2028..2143
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
promoter 2338..3519
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2568..3510
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3467..3487
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3544..3553
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 4165..4188
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 4225..4248
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 4260..4833
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4427..4444)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4654..4673
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4866..5465
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(4866..4885)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 5362..5382
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 5479..6067
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5532..5552)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5950..5961)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6274..6907
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7035..7051)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7035..7051)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7048..7070)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7059..7075
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7083..7113)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7128..7149
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7266..7283)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7437..8025)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7517..7536)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8196..9056)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8819..8838
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9057..9161)
/gene="bla"
/label=AmpR promoter
polyA_signal 9209..9343
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9246..9265)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9300..9319
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
3541 gccgccacca tggcaatcgc atctgaattt tctagtctcc cctcctatgc agcgtttgca
3601 actgcccagg aggcatacga gcaggctgtt gcgaacggcg actccgaagt ggtgctgaag
3661 aagctgaaaa agtctctgaa cgtcgcaaaa tccgagtttg atagagatgc agcaatgcaa
3721 agaaagctgg agaaaatggc agatcaagct atgacacaaa tgtacaagca ggcccgctca
3781 gaggataaac gagctaaagt tacctccgca atgcaaacta tgctgttcac catgctgaga
3841 aagctggaca acgacgcgct gaacaatata attaataatg cgcgggacgg gtgcgtaccc
3901 ttgaatatta ttccccttac gactgccgcg aagctcatgg ttgtcatacc agattataac
3961 acttacaaga atacgtgtga tggtacgacg ttcacttatg cgtctgcatt gtgggaaatt
4021 cagcaagtgg tagacgctga ttccaagata gtacagttgt ccgagattag catggacaac
4081 agtcccaatc tcgcttggcc tctgatcgtt accgcattga gggctaacag cgcagtcaaa
4141 ctgcagctcg aaggcggcgg gggatggagc catccacaat tcgagaaagg cggtggttca
4201 ggaggaggta gcgggggtgg atcatggtca catccgcagt ttgaaaagta aggatcccgc
4261 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
4321 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
4381 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
4441 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
4501 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
4561 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
4621 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
4681 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
4741 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
4801 gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccaca aggagacgac
4861 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
4921 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
4981 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
5041 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
5101 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
5161 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
5221 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
5281 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
5341 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
5401 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
5461 cctagacgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
5521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
5581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
5641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
5701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
5761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
5821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
5881 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
5941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
6001 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
6061 tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca atcacaagta
6121 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
6181 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
6241 atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac tcccaacgaa
6301 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
6361 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
6421 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
6481 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
6541 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
6601 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
6661 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
6721 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
6781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
6841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
6901 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
6961 atatcagaga gtgagaggcc ttgacattgc tagcgtttac cgtcgacctc tagctagagc
7021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
7081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
7141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
7201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
7261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
7381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
7441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
7501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
7561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
7621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
7681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
7741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
7921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
7981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
8041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
8101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
8161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
8221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
8281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
8341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
8401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
8461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
8521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
8581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
8641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
8701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
8761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
8821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
8881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
8941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
9001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
9061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
9121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
9181 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa
9241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
9301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
9361 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
9421 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
9481 ttgttaaata tgtactacaa acttagtagt
//
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