| 质粒名称: | pCMV-PE2-P2A-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 132776 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PE2-P2A-GFP,Cas9(H840A)-M-MLVrt(D200N, T306K, W313F, T330P, L603W)-P2A-GFP |
| 物种来源: | Synthetic; Cas9 is from S. pyogenes; M-MLV RT is from the Moloney murine leukemia virus |
| 插入大小: | 7170 bp |
| 基因突变: | See manuscript |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: |
SV40 NLS（主干上的N终端）
SV40 NLS（主干上的C终端）
|
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7-forward |
| 3' 测序引物 | BGH-reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-PE2-P2A-GFP was a gift from David Liu (Addgene plasmid # 132776 ; http://n2t.net/addgene:132776 ; RRID:Addgene_132776) |
| 参考文献部分: | Search-and-replace genome editing without double-strand breaks or donor DNA. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR. Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. 10.1038/s41586-019-1711-4 PubMed 31634902 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-PE2-P2A-GFP 10569 bp ds-DNA circular SYN 17-MAR-2020
DEFINITION Prime editing in mammalian cells with co-translational GFP
expression.
ACCESSION .
VERSION .
KEYWORDS pCMV-PE2-P2A-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10569)
AUTHORS Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM,
Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
TITLE Search-and-replace genome editing without double-strand breaks or
donor DNA.
JOURNAL Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi:
10.1038/s41586-019-1711-4.
PUBMED 31634902
REFERENCE 2 (bases 1 to 10569)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 17, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10569
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 282..870
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 771..790
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1024..1041
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 1158..1179
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 1194..1224
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 1232..1248
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 1237..1259
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 1256..1272
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1256..1272
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
polyA_signal 1343..1567
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 1556..1573
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(1596..1613)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS complement(1622..1642)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(1655..2371)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1697..1718)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 2046..2065
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2305..2326
/label=EGFP-N
/note="EGFP, reverse primer"
CDS complement(2372..2428)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(2438..2458)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(4631..8731)
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(H840A)
/note="generates RNA-guided single strand nicks in DNA due
to the H840A mutation in the HNH catalytic domain"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(8732..8752)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(8801..8820)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(8802..8820)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(8862..9065)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(8891..8911)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 9066..9445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 9617..9636
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 9715..9819
/gene="bla"
/label=AmpR promoter
CDS 9820..111
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(10038..10057)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
61 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
121 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
181 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
241 cactgagtgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
301 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
361 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
421 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
481 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
541 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
601 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
661 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
721 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
781 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
841 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
901 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
961 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
1021 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
1081 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
1141 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccta ggctttacac
1201 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
1261 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat cgagccccag
1321 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
1381 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
1441 ctactcagac aatgcgatgc aattttctca ttttattagg aaaggacagt gggagtggca
1501 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
1561 ggcacagtcg aggctgatca gcgggtttaa actcaatggt gatggtgatg atgaccggtt
1621 agactttcct cttcttcttg ggagaaccac cagacttgta cagctcgtcc atgccgagag
1681 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
1741 ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
1801 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
1861 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
1921 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
1981 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
2041 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
2101 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
2161 actgcactcc ataggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
2221 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
2281 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
2341 cggtgaacag ctcctcgccc ttgctcacca taggtccagg gttctcctcc acgtctccag
2401 cctgcttcag caggctgaag ttagtagctc cgcttccgac tttcctcttc ttcttgggct
2461 cgaattcgct gccgtcggcg gttctttttg agccgccaga gggtgatgaa ttttctatga
2521 ggagggtaga ggtgtctgga gtctctgtga tggctgcctt tcgggccgct tggtcagcca
2581 tccggttgcc tctagcctcg gcgctgtgtc ccttttgatg tcctggacaa tggattatgc
2641 taagtctttt gggcagaaag agggctttta gtagggccaa gatctcgtct ttatttttga
2701 tctctttgcc ttctgatgtg agccacccac gccttctgta tatttctcca tggatatggg
2761 cagtagcaaa agcataacgg ctatcagtat aaacatttag cttcttacct tctgccatct
2821 ttagggcctg ggtgagtgct atcagttcag cccgctgagc ggatgtcccg gctggcaggg
2881 ctttagccca gattacctcg gtctcggtgg tcaccgcagc tcccgcctta cgctgtccct
2941 cttgtaagag actgcttcca tccgtgtacc aggtgtggtc ggcgtctggg agcggctggt
3001 ccgttaggtc gggtcgggtt ccgtgggctt cggccaggat atcaaggcag ttgtgttgca
3061 gcccttcctc aggcagtggg agcagcgtag ccgggttcag ggctaccacc ggtccgaact
3121 ggacccggtc cgtgtccaaa agcaaggcct gatagtgagt catccgggcg ttggaaagcc
3181 agcggtcggg gggttgtttg actagtgcct ctactgcatg gggggccaga atgactagtg
3241 gctgtcccat ggttagcttg cctgcatcct ttgtcagtac ggcaatggct gctaccatcc
3301 gtaggcaagg gggccaccca gctgctactg ggtctagctt tttggacagg taggccaccg
3361 gccgacgcca aggtcccagt ttttgcgtta ggacaccttt ggcgtagccc tgcttctcgt
3421 cgacaaagag ttcaaagggc ttagtcaaat ctggcaaccc cagggctggg gcagttagaa
3481 gagcttgctt gatttcttga taggcctttt gttggtctgg gccccaatta aacagagtcc
3541 ccggtttggt gagagggtac aggggggctg ccatttctgc aaacccaggg atgaagaggc
3601 gacagaagcc tgccttccct aggaactccc ttagttgtcg aggggtcttc ggagtaggct
3661 gccccatcac agtctctttt ctggcctcag tcagccatct ctgaccctct tttagaagat
3721 accccagata cttgacctgt ttctggcaaa tttgggcttt cttggccgag gcccgatacc
3781 cgaggttccc tagggtttgt aacagggccc gagtaccttg ttggcagtct agctcagaag
3841 tggcggccag cagtaagtca tccacgtact gtagcaggat caagtctggg tgctggatcc
3901 ggaagtctgc taggtctctg tgcagtgcct cattaaacag ggtgggactg tttttgaaac
3961 cctgtgggag tctggtccag gtcaattgtc ctgagattcc catctctgga tctctccact
4021 caaaggcgaa gagaggctga ctggtggggt ggagtctcag gcagaaaaag gcatccttta
4081 aatcaagcac agtgtaccac tggtgggacg gtgggagccc gctcaagagg ttgtaagggt
4141 tgggcacggt ggggtggatg tcttccaccc gcttgttgac ttctctcaga tcctggacag
4201 gcctataatc attagtccct ggtttcttaa cgggtagcag gggcgtgttc cagggggact
4261 ggcagggtac cagtattccc tggtccaaca gtctctgtat gtggggcttg atccccagtc
4321 tggcttcttg tgacatgggg tattgtttta tggacacggg ggtagaggtt gctttcagag
4381 gtatgatcag aggagcttgg cgaactgcca gtcccatgcc cccggtttcc gcccaggcct
4441 gaggaaaatc agacagccat gtggacccta gagaaacatc tggctctttt gaggtctcat
4501 gtagccgata ctcatcttct atatttaggg tgctgctgcc gccgctgctg ccgccactgc
4561 tctctggtgt tgctgactcg cttgttcctg gtgtctcgct gccagaggat cctccgctag
4621 atcctccaga gtcacctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga
4681 tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt
4741 cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc cagattggtc agggtaaaca
4801 ggtggatgat attctcggcc tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg
4861 acagcacttt gtccagatta gcgtcggcca ggatcactct cttggagaac tcgctgatct
4921 gctcgatgat ctcgtccagg tagtgcttgt gctgttccac aaacagctgt ttctgctcat
4981 tatcctcggg ggagcccttc agcttctcat agtggctggc caggtacagg aagttcacat
5041 atttggaggg cagggccagt tcgtttccct tctgcagttc gccggcagag gccagcattc
5101 tcttccggcc gttttccagc tcgaacaggg agtacttagg cagcttgatg atcaggtcct
5161 ttttcacttc tttgtagccc ttggcttcca gaaagtcgat gggattcttc tcgaagctgc
5221 ttctttccat gatggtgatc cccagcagct ctttcacact cttcagtttc ttggacttgc
5281 ccttttccac tttggccacc accagcacag aataggccac ggtggggctg tcgaagccgc
5341 cgtacttctt agggtcccag tccttctttc tggcgatcag cttatcgctg ttcctcttgg
5401 gcaggataga ctctttgctg aagccgcctg tctgcacctc ggtctttttc acgatattca
5461 cttggggcat gctcagcact ttccgcacgg tggcaaaatc ccggccctta tcccacacga
5521 tctccccggt ttcgccgttt gtctcgatca gaggccgctt ccggatctcg ccgttggcca
5581 gggtaatctc ggtcttgaaa aagttcatga tgttgctgta gaagaagtac ttggcggtag
5641 ccttgccgat ttcctgctcg ctcttggcga tcatcttccg cacgtcgtac accttgtagt
5701 cgccgtacac gaactcgctt tccagcttag ggtacttttt gatcagggcg gttcccacga
5761 cggcgttcag gtaggcgtcg tgggcgtggt ggtagttgtt gatctcgcgc actttgtaaa
5821 actggaaatc cttccggaaa tcggacacca gcttggactt cagggtgatc actttcactt
5881 cccggatcag cttgtcattc tcgtcgtact tagtgttcat ccgggagtcc aggatctgtg
5941 ccacgtgctt tgtgatctgc cgggtttcca ccagctgtct cttgatgaag ccggccttat
6001 ccagttcgct caggccgcct ctctcggcct tggtcagatt gtcgaacttt ctctgggtaa
6061 tcagcttggc gttcagcagc tgccgccagt agttcttcat cttcttcacg acctcttcgg
6121 agggcacgtt gtcgctcttg ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga
6181 tggagtcgtc cttcagaaag ctctgaggca cgatagcgtc cacatcgtag tcggacagcc
6241 ggttgatgtc cagttcctgg tccacgtaca tatcccgccc attctgcagg tagtacaggt
6301 acagcttctc gttctgcagc tgggtgtttt ccacggggtg ttctttcagg atctggctgc
6361 ccagctcttt gatgccctct tcgatccgct tcattctctc gcggctgttc ttctgtccct
6421 tctgggtggt ctggttctct ctggccattt cgatcacgat gttctcgggc ttgtgccggc
6481 ccatcacttt cacgagctcg tccaccacct tcactgtctg caggatgccc ttcttaatgg
6541 cggggctgcc ggccagattg gcaatgtgct cgtgcaggct atcgccctgg ccggacacct
6601 gggctttctg gatgtcctct ttaaaggtca ggctgtcgtc gtggatcagc tgcatgaagt
6661 ttctgttggc gaagccgtcg gacttcagga aatccaggat tgtcttgccg gactgcttgt
6721 cccggatgcc gttgatcagc ttccggctca gcctgcccca gccggtgtat ctccgccgct
6781 tcagctgctt catcactttg tcgtcgaaca ggtgggcata ggttttcagc cgttcctcga
6841 tcatctctct gtcctcaaac agtgtcaggg tcagcacgat atcttccaga atgtcctcgt
6901 tttcctcatt gtccaggaag tccttgtcct tgataatttt cagcagatcg tggtatgtgc
6961 ccagggaggc gttgaaccga tcttccacgc cggagatttc cacggagtcg aagcactcga
7021 ttttcttgaa gtagtcctct ttcagctgct tcacggtcac tttccggttg gtcttgaaca
7081 gcaggtccac gatggccttt ttctgctcgc cgctcaggaa ggcgggcttt ctcattccct
7141 cggtcacgta tttcactttg gtcagctcgt tatacacggt gaagtactcg tacagcaggc
7201 tgtgcttggg cagcaccttc tcgttgggca ggttcttatc gaagttggtc atccgctcga
7261 tgaagctctg ggcggaagcg cccttgtcca ccacttcctc gaagttccag ggggtgatgg
7321 tttcctcgct ctttctggtc atccaggcga atctgctgtt tcccctggcc agagggccca
7381 cgtagtaggg gatgcggaag gtcaggatct tctcgatctt ttcccggttg tccttcagga
7441 atgggtaaaa atcttcctgc cgccgcagaa tggcgtgcag ctctcccagg tggatctggt
7501 gggggatgct gccgttgtcg aaggtccgct gcttccgcag caggtcctct ctgttcagct
7561 tcacgagcag ttcctcggtg ccgtccatct tttccaggat gggcttgatg aacttgtaga
7621 actcttcctg gctggctccg ccgtcaatgt agccggcgta gccgttcttg ctctggtcga
7681 agaaaatctc tttgtacttc tcaggcagct gctgccgcac gagagctttc agcagggtca
7741 ggtcctggtg gtgctcgtcg tatctcttga tcatagaggc gctcaggggg gccttggtga
7801 tctcggtgtt cactctcagg atgtcgctca gcaggatggc gtcggacagg ttcttggcgg
7861 ccagaaacag gtcggcgtac tggtcgccga tctgggccag caggttgtcc aggtcgtcgt
7921 cgtaggtgtc cttgctcagc tgcagtttgg catcctcggc caggtcgaag ttgctcttga
7981 agttgggggt caggcccagg ctcagggcaa tcaggtttcc gaacaggcca ttcttcttct
8041 cgccgggcag ctgggcgatc agattttcca gccgtctgct cttgctcagt ctggcagaca
8101 ggatggcctt ggcgtccacg ccgctggcgt tgatggggtt ttcctcgaac agctggttgt
8161 aggtctgcac cagctggatg aacagcttgt ccacgtcgct gttgtcgggg ttcaggtcgc
8221 cctcgatcag gaagtggccc cggaacttga tcatgtgggc cagggccaga tagatcagcc
8281 gcaggtcggc cttgtcggtg ctgtccacca gtttctttct caggtggtag atggtggggt
8341 acttctcgtg gtaggccacc tcgtccacga tgttgccgaa gatggggtgc cgctcgtgct
8401 tcttatcctc ttccaccagg aaggactctt ccagtctgtg gaagaagctg tcgtccacct
8461 tggccatctc gttgctgaag atctcttgca gatagcagat ccggttcttc cgtctggtgt
8521 atcttcttct ggcggttctc ttcagccggg tggcctcggc tgtttcgccg ctgtcgaaca
8581 gcagggctcc gatcaggttc ttcttgatgc tgtgccggtc ggtgttgccc agcaccttga
8641 atttcttgct gggcaccttg tactcgtcgg tgatcacggc ccagcccaca gagttggtgc
8701 cgatgtccag gccgatgctg tacttcttgt cgactttccg cttcttcttt ggtgactcga
8761 actcgcttcc gtcggctgtc cgtttcatgg tggcggctct ccctatagtg agtcgtatta
8821 gcggccgcgg atctctagcg gatctgacgg ttcactaaac cagctctgct tatatagacc
8881 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
8941 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
9001 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
9061 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
9121 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
9181 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
9241 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
9301 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
9361 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
9421 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
9481 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
9541 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
9601 tactggctta actatgcggc atcagagcag attgtactga gagtagaccc taggggatcg
9661 ggagatcgat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
9721 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
9781 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
9841 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
9901 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
9961 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
10021 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
10081 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
10141 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
10201 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
10261 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
10321 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
10381 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
10441 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
10501 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
10561 gggccagat
//
