| 质粒名称: | pDY0216 PASTEv1 PE2-P2A-Bxb1 |
|---|---|
| 目录编号: | 179104 |
| 存储实验室: | Omar Abudayyeh, Jonathan Gootenberg |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体抗性: | Ampicillin, 100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | pCMV-PE2-P2A-Bxb1 |
|
文献引用方法 |
|
| 材料和方法部分: | pDY0216 PASTEv1 PE2-P2A-Bxb1 was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 179104 ; http://n2t.net/addgene:179104 ; RRID:Addgene_179104) |
| 参考文献部分: | Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases. Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS. Nat Biotechnol. 2022 Nov 24. doi: 10.1038/s41587-022-01527-4. 10.1038/s41587-022-01527-4 PubMed 36424489 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pDY0216 PASTEv1 PE2-P2A-Bxb1 11344 bp ds-DNA circular SYN 10-FEB-2022
DEFINITION PASTEv1 PE2-P2A-Bxb1.
ACCESSION .
VERSION .
KEYWORDS pDY0216 PASTEv1 PE2-P2A-Bxb1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11344)
AUTHORS Ioannidi EI, Yarnall MTN, Schmitt-Ulms C, Krajeski RN, Lim J,
Villiger L, Zhou W, Jiang K, Roberts N, Zhang L, Vakulskas CA,
Walker II JA, Kadina AP, Zepeda AE, Holden K, Gootenberg JS,
Abudayyeh OO
TITLE Drag-and-drop genome insertion without DNA cleavage with
CRISPR-directed integrases
JOURNAL bioRxiv 2021.11.01.466786
REFERENCE 2 (bases 1 to 11344)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 10, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..11344
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(70..89)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 261..640
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 641..844
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 795..815
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 886..905
/label=T7
/note="T7 promoter, forward primer"
promoter 886..904
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 954..974
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 975..5075
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(H840A)
/note="generates RNA-guided single strand nicks in DNA due
to the H840A mutation in the HNH catalytic domain"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 7248..7268
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7278..7334
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 7335..7355
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7356..7382
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(8908..8925)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8914..9138
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(9209..9225)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9209..9225)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9222..9244)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9233..9249
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9257..9287)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9302..9323
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9440..9457)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9611..10199)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9691..9710)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10370..11230)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10993..11012
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11231..11335)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ccgaaaagtg ccacctgacg tcgacggatc gggagatcga tctcccgatc ccctagggtc
61 tactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
121 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
181 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
241 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
301 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
361 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
421 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
481 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
541 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
601 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
661 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
721 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
781 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
841 agctggttta gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta
901 tagggagagc cgccaccatg aaacggacag ccgacggaag cgagttcgag tcaccaaaga
961 agaagcggaa agtcgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
1021 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
1081 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
1141 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
1201 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
1261 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
1321 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
1381 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
1441 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
1501 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
1561 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
1621 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
1681 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
1741 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
1801 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
1861 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
1921 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
1981 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
2041 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
2101 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
2161 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
2221 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
2281 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
2341 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
2401 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
2461 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
2521 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
2581 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
2641 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
2701 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
2761 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
2821 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
2881 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
2941 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
3001 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
3061 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
3121 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
3181 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
3241 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
3301 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
3361 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
3421 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
3481 atgtggacgc tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
3541 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
3601 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
3661 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
3721 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
3781 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
3841 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
3901 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
3961 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
4021 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
4081 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
4141 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
4201 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
4261 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
4321 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
4381 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
4441 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
4501 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
4561 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
4621 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
4681 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
4741 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
4801 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
4861 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
4921 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
4981 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
5041 agacacggat cgacctgtct cagctgggag gtgactctgg aggatctagc ggaggatcct
5101 ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca
5161 gcggcggcag cagcacccta aatatagaag atgagtatcg gctacatgag acctcaaaag
5221 agccagatgt ttctctaggg tccacatggc tgtctgattt tcctcaggcc tgggcggaaa
5281 ccgggggcat gggactggca gttcgccaag ctcctctgat catacctctg aaagcaacct
5341 ctacccccgt gtccataaaa caatacccca tgtcacaaga agccagactg gggatcaagc
5401 cccacataca gagactgttg gaccagggaa tactggtacc ctgccagtcc ccctggaaca
5461 cgcccctgct acccgttaag aaaccaggga ctaatgatta taggcctgtc caggatctga
5521 gagaagtcaa caagcgggtg gaagacatcc accccaccgt gcccaaccct tacaacctct
5581 tgagcgggct cccaccgtcc caccagtggt acactgtgct tgatttaaag gatgcctttt
5641 tctgcctgag actccacccc accagtcagc ctctcttcgc ctttgagtgg agagatccag
5701 agatgggaat ctcaggacaa ttgacctgga ccagactccc acagggtttc aaaaacagtc
5761 ccaccctgtt taatgaggca ctgcacagag acctagcaga cttccggatc cagcacccag
5821 acttgatcct gctacagtac gtggatgact tactgctggc cgccacttct gagctagact
5881 gccaacaagg tactcgggcc ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg
5941 ccaagaaagc ccaaatttgc cagaaacagg tcaagtatct ggggtatctt ctaaaagagg
6001 gtcagagatg gctgactgag gccagaaaag agactgtgat ggggcagcct actccgaaga
6061 cccctcgaca actaagggag ttcctaggga aggcaggctt ctgtcgcctc ttcatccctg
6121 ggtttgcaga aatggcagcc cccctgtacc ctctcaccaa accggggact ctgtttaatt
6181 ggggcccaga ccaacaaaag gcctatcaag aaatcaagca agctcttcta actgccccag
6241 ccctggggtt gccagatttg actaagccct ttgaactctt tgtcgacgag aagcagggct
6301 acgccaaagg tgtcctaacg caaaaactgg gaccttggcg tcggccggtg gcctacctgt
6361 ccaaaaagct agacccagta gcagctgggt ggcccccttg cctacggatg gtagcagcca
6421 ttgccgtact gacaaaggat gcaggcaagc taaccatggg acagccacta gtcattctgg
6481 ccccccatgc agtagaggca ctagtcaaac aaccccccga ccgctggctt tccaacgccc
6541 ggatgactca ctatcaggcc ttgcttttgg acacggaccg ggtccagttc ggaccggtgg
6601 tagccctgaa cccggctacg ctgctcccac tgcctgagga agggctgcaa cacaactgcc
6661 ttgatatcct ggccgaagcc cacggaaccc gacccgacct aacggaccag ccgctcccag
6721 acgccgacca cacctggtac acggatggaa gcagtctctt acaagaggga cagcgtaagg
6781 cgggagctgc ggtgaccacc gagaccgagg taatctgggc taaagccctg ccagccggga
6841 catccgctca gcgggctgaa ctgatagcac tcacccaggc cctaaagatg gcagaaggta
6901 agaagctaaa tgtttatact gatagccgtt atgcttttgc tactgcccat atccatggag
6961 aaatatacag aaggcgtggg tggctcacat cagaaggcaa agagatcaaa aataaagacg
7021 agatcttggc cctactaaaa gccctctttc tgcccaaaag acttagcata atccattgtc
7081 caggacatca aaagggacac agcgccgagg ctagaggcaa ccggatggct gaccaagcgg
7141 cccgaaaggc agccatcaca gagactccag acacctctac cctcctcata gaaaattcat
7201 caccctctgg cggctcaaaa agaaccgccg acggcagcga attcgagccc aagaagaaga
7261 ggaaagtcgg aagcggagct actaacttca gcctgctgaa gcaggctggc gacgtggagg
7321 agaaccctgg acctccaaaa aagaaaagaa aagtgtatcc ctatgatgtc cccgattatg
7381 ccggttcaag agccctggtc gtgattagac tgagccgagt gacagacgcc accacaagtc
7441 ccgagagaca gctggaatca tgccagcagc tctgtgctca gcggggttgg gatgtggtcg
7501 gcgtggcaga ggatctggac gtgagcgggg ccgtcgatcc attcgacaga aagaggaggc
7561 ccaacctggc aagatggctc gctttcgagg aacagccctt tgatgtgatc gtcgcctaca
7621 gagtggaccg gctgacccgc tcaattcgac atctccagca gctggtgcat tgggctgagg
7681 accacaagaa actggtggtc agcgcaacag aagcccactt cgatactacc acaccttttg
7741 ccgctgtggt catcgcactg atgggcactg tggcccagat ggagctcgaa gctatcaagg
7801 agcgaaacag gagcgcagcc catttcaata ttagggccgg taaatacaga ggctccctgc
7861 ccccttgggg atatctccct accagggtgg atggggagtg gagactggtg ccagaccccg
7921 tccagagaga gcggattctg gaagtgtacc acagagtggt cgataaccac gaaccactcc
7981 atctggtggc acacgacctg aatagacgcg gcgtgctctc tccaaaggat tattttgctc
8041 agctgcaggg aagagagcca cagggaagag aatggagtgc tactgcactg aagagatcta
8101 tgatcagtga ggctatgctg ggttacgcaa cactcaatgg caaaactgtc cgggacgatg
8161 acggagcccc tctggtgagg gctgagccta ttctcaccag agagcagctc gaagctctgc
8221 gggcagaact ggtcaagact agtcgcgcca aacctgccgt gagcacccca agcctgctcc
8281 tgagggtgct gttctgcgcc gtctgtggag agccagcata caagtttgcc ggcggagggc
8341 gcaaacatcc ccgctatcga tgcaggagca tggggttccc taagcactgt ggaaacggga
8401 cagtggccat ggctgagtgg gacgcctttt gcgaggaaca ggtgctggat ctcctgggtg
8461 acgctgagcg gctggaaaaa gtgtgggtgg caggatctga ctccgctgtg gagctggcag
8521 aagtcaatgc cgagctcgtg gatctgactt ccctcatcgg atctcctgca tatagagctg
8581 ggtccccaca gagagaagct ctggacgcac gaattgctgc actcgctgct agacaggagg
8641 aactggaggg cctggaggcc aggccctctg gatgggagtg gcgagaaacc ggacagaggt
8701 ttggggattg gtggagggag caggacaccg cagccaagaa cacatggctg agatccatga
8761 atgtccggct cacattcgac gtgcgcggtg gcctgactcg aaccatcgat tttggcgacc
8821 tgcaggagta tgaacagcac ctgagactgg ggtccgtggt cgaaagactg cacactggga
8881 tgtcctaggt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
8941 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
9001 cctaataaaa tgagaaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
9061 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
9121 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga
9181 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
9241 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct
9301 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
9361 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
9421 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
9481 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
9541 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
9601 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
9661 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
9721 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
9781 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
9841 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
9901 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
9961 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
10021 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
10081 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
10141 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
10201 aagatccttt gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag
10261 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
10321 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
10381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
10441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
10501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
10561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
10621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
10681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
10741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
10801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
10861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
10921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
10981 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
11041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
11101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
11161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
11221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
11281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
11341 ttcc
//
