出品公司: | -- |
---|---|
载体名称: | pHT304 |
质粒类型: | 枯草芽孢杆菌表达载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | -- |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | -- |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄和红霉素 |
筛选标记: | -- |
备注: | -- |
产品目录号: | -- |
稳定性: | 稳定 |
组成型: | 非组成型 |
病毒/非病毒: | 非病毒 |
买家导航
pHT304载体质粒基本信息
pHT304质粒图谱载体图谱和pHT304载体序列质粒序列多克隆位点信息
pHT304质粒载体简介
pHT304质粒序列载体序列
LOCUS pHT304 6530 bp DNA circular 8-FEB-2014 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| BASE COUNT 1954 a 1323 c 1404 g 1849 t ORIGIN 1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc 61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag 121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga 181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt 241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac 301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg 361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc 421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat 481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg 541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat 601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact 661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac 721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg 781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat 841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc 901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt 961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga 1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg 1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat 1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg 1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg 1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca 1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag 1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc 1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa 1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa 1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta 1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg 1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta 1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag 1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt 1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat 1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg 1981 cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat 2041 accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga 2101 agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg 2161 tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata 2221 tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga 2281 tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga 2341 attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc 2401 aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt 2461 aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg 2521 atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat 2581 aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg 2641 ttgatcccct tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt 2701 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt 2761 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac 2821 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat 2881 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta 2941 tttacagatg taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa 3001 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa 3061 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa 3121 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgacagaaa 3181 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc 3241 ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta 3301 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat 3361 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct 3421 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca 3481 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat 3541 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc 3601 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc 3661 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt 3721 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat 3781 atactactga cagcttccaa gaaggtaaag aggtccctag cgcctacggg gaatttgtat 3841 cgggattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 5701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 5761 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 5821 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 5881 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc 5941 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 6001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 6061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg 6121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac 6181 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 6241 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 6301 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 6361 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 6481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat //