pHT304

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pHT304
质粒类型: 枯草芽孢杆菌表达载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: --
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄和红霉素
筛选标记: --
备注: --
产品目录号: --
稳定性: 稳定
组成型: 非组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pET47b载体图谱

载体简介

载体序列

LOCUS       pHT304                  6530 bp    DNA     circular      8-FEB-2014
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
BASE COUNT     1954 a      1323 c      1404 g      1849 t 
ORIGIN
        1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc 
       61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag 
      121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga 
      181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt 
      241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac 
      301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg 
      361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc 
      421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat 
      481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg 
      541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat 
      601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact 
      661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac 
      721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg 
      781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat 
      841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc 
      901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt 
      961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga 
     1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg 
     1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat 
     1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg 
     1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg 
     1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca 
     1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag 
     1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc 
     1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa 
     1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa 
     1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta 
     1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg 
     1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta 
     1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag 
     1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt 
     1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat 
     1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg 
     1981 cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat 
     2041 accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga 
     2101 agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg 
     2161 tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata 
     2221 tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga 
     2281 tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga 
     2341 attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc 
     2401 aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt 
     2461 aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg 
     2521 atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat 
     2581 aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg 
     2641 ttgatcccct tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt 
     2701 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt 
     2761 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac 
     2821 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat 
     2881 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta 
     2941 tttacagatg taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa 
     3001 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa 
     3061 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa 
     3121 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgacagaaa 
     3181 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc 
     3241 ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta 
     3301 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat 
     3361 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct 
     3421 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca 
     3481 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat 
     3541 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc 
     3601 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc 
     3661 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt 
     3721 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat 
     3781 atactactga cagcttccaa gaaggtaaag aggtccctag cgcctacggg gaatttgtat 
     3841 cgggattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 
     3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 
     3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 
     4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 
     4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 
     4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 
     4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 
     4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 
     4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 
     4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 
     4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 
     4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 
     4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc 
     4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 
     4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 
     4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 
     4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 
     4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 
     4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 
     4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 
     5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 
     5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 
     5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 
     5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 
     5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 
     5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 
     5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 
     5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 
     5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 
     5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 
     5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg 
     5701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 
     5761 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt 
     5821 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat 
     5881 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc 
     5941 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 
     6001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 
     6061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg 
     6121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac 
     6181 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 
     6241 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 
     6301 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 
     6361 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 
     6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 
     6481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 
//

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