| 质粒名称: | pLXSN18E6E7 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 53459 |
| 存储实验室: | Denise Galloway |
|
载体骨架基本信息 |
|
| 载体名称: | pLXSN |
| 载体出品公司: | Clontech |
| 空载体大小: | 5884bp |
| 完整质粒大小: | 6688bp |
| 载体类型: | Retroviral |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | HPV18E6E7 |
| 插入大小: | 804bp |
| GenBank编号 | AY262282 X05015 |
| 启动子: | CMV |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI (not destroyed) |
| 3’克隆位点: | XhoI (not destroyed) |
| 5’测序引物 | pLSXNf |
| 3’测序引物 | pLXSNr |
|
文献引用方法 |
|
| 材料和方法部分: | pLXSN18E6E7 was a gift from Denise Galloway (Addgene plasmid # 53459 ; http://n2t.net/addgene:53459 ; RRID:Addgene_53459) |
| 参考文献部分: | The E7 gene of human papillomavirus type 16 is sufficient for immortalization of human epithelial cells. Halbert CL, Demers GW, Galloway DA. J Virol. 1991 Jan;65(1):473-8. PubMed 1845902 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLXSN18E6E7 6688 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant
retrovirus providing efficient gene transfers and expression of the
HPV18 E6 and E7 genes..
ACCESSION .
VERSION .
KEYWORDS pLXSN18E6E7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6688)
AUTHORS Halbert CL, Demers GW, Galloway DA
TITLE The E7 gene of human papillomavirus type 16 is sufficient for
immortalization of human epithelial cells.
JOURNAL J Virol. 1991 Jan;65(1):473-8.
PUBMED 1845902
REFERENCE 2 (bases 1 to 6688)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6688
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 588..763
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 826..1025
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1226..1642
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1572..1594
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1610..1626
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2486..2506)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2491..2820
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2671..2806
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2733..2752
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2880..3674
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2934..2953)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3544..3563
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
LTR 3734..4327
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(4430..4452)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 4538..4678
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4593..4612
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4693..4710)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4864..5452)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4944..4963)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5623..6483)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6246..6265
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6484..6588)
/gene="bla"
/label=AmpR promoter
primer_bind 6656..6674
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
121 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgcttttgaa
181 agaccccacc cgtaggtggc aagctagctt aagtaacgcc actttgcaag gcatggaaaa
241 atacataact gagaatagaa aagttcagat caaggtcagg aacaaagaaa cagctgaata
301 ccaaacagga tatctgtggt aagcggttcc tgccccggct cagggccaag aacagatgag
361 acagctgagt gatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcggg
421 gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag tgaatcatca
481 gatgtttcca gggtgcccca aggacctgaa aatgaccctg taccttattt gaactaacca
541 atcagttcgc ttctcgcttc tgttcgcgcg cttccgctct ccgagctcaa taaaagagcc
601 cacaacccct cactcggcgc gccagtcttc cgatagactg cgtcgcccgg gtacccgtat
661 tcccaataaa gcctcttgct gtttgcatcc gaatcgtggt ctcgctgttc cttgggaggg
721 tctcctctga gtgattgact acccacgacg ggggtctttc atttgggggc tcgtccggga
781 tttggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac
841 ttatctgtgt ctgtccgatt gtctagtgtc tatgtttgat gttatgcgcc tgcgtctgta
901 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
961 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
1021 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
1081 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
1141 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
1201 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
1261 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
1321 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
1381 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
1441 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
1501 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
1561 cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc tttatccagc
1621 cctcactcct tctctaggcg ccggaattca acacaccaca atactatggc gcgctttgag
1681 gatccaacac ggcgacccta caagctacct gatctgtgca cggaactgaa cacttcactg
1741 caagacatag aaataacctg tgtatattgc aagacagtat tggaacttac agaggtattt
1801 gaatttgcat ttaaagattt atttgtggtg tatagagaca gtataccgca tgctgcatgt
1861 cataaatgta tagattttta ttctagaatt agagaattaa gacattattc agactctgtg
1921 tatggagaca cattggaaaa actaactaac actgggttat acaatttatt aataaggtgc
1981 ctgcggtgcc agaaaccgtt gaatccagca gaaaaactta gacaccttaa tgaaaaacga
2041 cgatttcaca acatagctgg gcactataga ggccagtgcc attcgtgctg caaccgagca
2101 cgacaggaac gactccaacg acgcagagaa acacaagtat aatattaagt atgcatggac
2161 ctaaggcaac attgcaagac attgtattgc atttagagcc ccaaaatgaa attccggttg
2221 accttctatg tcacgagcaa ttaagcgact cagaggaaga aaacgatgaa atagatggag
2281 ttaatcatca acatttacca gcccgacgag ccgaaccaca acgtcacaca atgttgtgta
2341 tgtgttgtaa gtgtgaagcc agaattgagc tagtagtaga aagctcagca gacgaccttc
2401 gagcattcca gcagctgttt ctgaacaccc tgtcctttgt gtgtccgtgg tgtgcatccc
2461 agcagtaact cgaggatccg gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
2521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
2581 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
2641 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
2701 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
2761 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
2821 aagcttgggc tgcaggtcga ggcggatctg atcaagagac aggatgagga tcgtttcgca
2881 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
2941 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
3001 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
3061 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
3121 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
3181 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
3241 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
3301 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
3361 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
3421 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
3481 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
3541 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
3601 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
3661 acgagttctt ctgagcggga ctctggggtt cgataaaata aaagatttta tttagtctcc
3721 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
3781 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
3841 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
3901 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
3961 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
4021 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
4081 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
4141 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
4201 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
4261 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
4321 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
4381 ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
4441 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
4501 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
4561 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
4621 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
4681 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
4741 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
4801 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
4861 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
4921 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
4981 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
5041 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
5101 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
5161 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
5221 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
5281 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
5341 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
5401 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
5461 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
5521 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
5581 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
5641 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
5701 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
5761 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
5821 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
5881 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc
5941 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
6001 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
6061 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
6121 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
6181 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac
6241 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
6301 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
6361 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
6421 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
6481 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
6541 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
6601 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
6661 gcgtatcacg aggccctttc gtcttcaa
//
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