| 出品公司: | |
|---|---|
| 载体名称: | pRARE |
| 质粒类型: | 稀有密码子表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | -- |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRARE 3301 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3301)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3301
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 274..302
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
tRNA 546..622
/gene="argU"
/product="E. coli tRNA-Arg recognizing AGA and AGG"
/label=argU
CDS complement(1867..2526)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter complement(2527..2629)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
ORIGIN
1 gggtgggtgc tactgaagga cagttttggt gactgcgctc ctccaagcca gttacctcgg
61 ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg
121 ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat
181 cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
241 agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg ataagcttta
301 atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa
361 caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtacaat tgacgacgac
421 aagggcccgg gcaaactagt aatcagacgc ggtcgttcac ttgttcagca accagatcac
481 aagccattga ctcagcaagg gttgaccgta taattcacgc gattacaccg cattgcggta
541 tcaacgcgcc cttagctcag ttggatagag caacgacctt ctaagtcgtg ggccgcaggt
601 tcgaatcctg cagggcgcgc cattacaatt caatcagtta cgccttcttt atatcctcca
661 ccatgggtgc tgacagacga gaagcgtttt atcgctaact gattaattat aaatcagtta
721 gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt attagcagcc acgagtcggc
781 acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg gggtcggagg ttcaaatcct
841 ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta cggttggttt ttttatatct
901 gcaattaatt cgataaacag accgtgacac atcacagcct gtttattttc tgttatcaga
961 acgtccagac cacaccctcg agtcgggcag cgttgggtcc tggccacggg tgcgcatgat
1021 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
1081 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
1141 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
1201 ccctacgtgc tgctgaagtt gcccgcaaca gagagtggaa ccaaccggtg ataccacgat
1261 actatgactg agagtcaacg ccatgagcgg cctcatttct tattctgagt tacaacagtc
1321 cgcaccgctg ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
1381 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc
1441 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc
1501 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
1561 aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta
1621 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca
1681 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
1741 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
1801 cttattatca cttattcagg cgtagcaacc aggcgtttaa gggcaccaat aactgcctta
1861 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
1921 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
1981 cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga agttgtccat
2041 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
2101 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
2161 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
2221 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
2281 cagctcaccg tctttcattg ccatacggaa ttccggatga gcattcatca ggcgggcaag
2341 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
2401 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
2461 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
2521 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
2581 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
2641 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
2701 tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc
2761 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
2821 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
2881 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
2941 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
3001 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
3061 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
3121 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
3181 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
3241 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgtctgcctt aacccggccc
3301 a
//
