出品公司: | Cell Biolabs, Inc. |
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载体名称: | pBABE-Puro SV40LT, pBABEpuro SV40LT, pBABE Puro SV40 Large T |
质粒类型: | 哺乳动物细胞逆病毒基因表达载体 |
插入基因: | SV40 LT, SV40gp6 |
插入基因Genbank ID: | AF316141 |
插入位点: | BamH I (5’端)/ BamH I (3'端) |
高拷贝/低拷贝: | 低拷贝 |
启动子: | PGK |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 7302 bp |
5' 测序引物及序列: | pBABE 5‘: CTTTATCCAGCCCTCAC |
3' 测序引物及序列: | pBABE 3’: ACCCTAACTGACACACATTCC |
载体标签: | -- |
载体抗性: | 氨苄 |
筛选标记: | 嘌呤霉素(Puromycin) |
备注: | 含有嘌呤霉素筛选基因,可以用来筛选感染稳定的细胞系。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
买家导航
pBABE-Puro SV40LT载体质粒基本信息
pBABE-Puro SV40LT质粒图谱载体图谱和pBABE-Puro SV40LT载体序列质粒序列多克隆位点信息
pBABE-Puro SV40LT质粒载体简介
pBABE-Puro SV40LT质粒序列载体序列
LOCUS pBABE-puro-SV40LT 7302 bp DNA circular 21-SEP-2014 DEFINITION pBABE-puro. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|709679084| COMMENT LSOWNER| COMMENT VNTNAME|pBABE-puro-SV40LT 5169| COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers misc_feature 12..479 /vntifkey="21" /label=5_LTR2 misc_feature 18..479 /vntifkey="21" /label=3MoMuLV_LTR misc_feature 549..1350 /vntifkey="21" /label=psi_plus_pack misc_feature 1318..1334 /vntifkey="21" /label=pBABE_5_primer misc_feature complement(3541..3561) /vntifkey="21" /label=pBABE_3_primer misc_feature complement(3547..3762) /vntifkey="21" /label=SV40_enhancer promoter 3559..3827 /vntifkey="30" /label=SV40_promoter rep_origin 3726..3803 /vntifkey="33" /label=SV40_origin misc_feature 3788..3807 /vntifkey="21" /label=SV40pro_F_primer CDS complement(3861..4535) /vntifkey="4" /label=ORF\frame\2 gene 3885..4484 /gene="puro (variant)" /vntifkey="60" CDS 3885..4484 /vntifkey="4" /label=ORF\frame\3 misc_feature 4583..5176 /vntifkey="21" /label=3MoMuLV_LTR misc_feature 4625..5176 /vntifkey="21" /label=5_LTR2 gene complement(6314..7174) /gene="Ampicillin" /vntifkey="60" CDS complement(6314..7174) /vntifkey="4" /label=ORF\frame\3 promoter complement(7216..7244) /vntifkey="30" /label=AmpR_promoter misc_feature 1361..3487 /vntifkey="21" /label=SV40LT /note="SV40 Large T antigen" BASE COUNT 1862 a 1815 c 1819 g 1806 t ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc atggataaag ttttaaacag 1381 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtgcct gggggaatat 1441 tcctctgatg agaaaggcat atttaaaaaa atgcaaggag tttcatcctg ataaaggagg 1501 agatgaagaa aaaatgaaga aaatgaatac tctgtacaag aaaatggaag atggagtaaa 1561 atatgctcat caacctgact ttggaggctt ctgggatgca actgagattc caacctatgg 1621 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga 1681 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa 1741 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag 1801 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa 1861 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca 1921 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc 1981 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa 2041 taaggaatat ttgatgtata gtgccttgac tagagatcca ttttctgtta ttgaggaaag 2101 tttgccaggt gggttaaagg agcatgattt taatccagaa gaagcagagg aaactaaaca 2161 agtgtcctgg aagcttgtaa cagagtatgc aatggaaaca aaatgtgatg atgtgttgtt 2221 attgcttggg atgtacttgg aatttcagta cagttttgaa atgtgtttaa aatgtattaa 2281 aaaagaacag cccagccact ataagtacca tgaaaagcat tatgcaaatg ctgctatatt 2341 tgctgacagc aaaaaccaaa aaaccatatg ccaacaggct gttgatactg ttttagctaa 2401 aaagcgggtt gatagcctac aattaactag agaacaaatg ttaacaaaca gatttaatga 2461 tcttttggat aggatggata taatgtttgg ttctacaggc tctgctgaca tagaagaatg 2521 gatggctgga gttgcttggc tacactgttt gttgcccaaa atggattcag tggtgtatga 2581 ctttttaaaa tgcatggtgt acaacattcc taaaaaaaga tactggctgt ttaaaggacc 2641 aattgatagt ggtaaaacta cattagcagc tgctttgctt gaattatgtg gggggaaagc 2701 tttaaatgtt aatttgccct tggacaggct gaactttgag ctaggagtag ctattgacca 2761 gtttttagta gtttttgagg atgtaaaggg cactggaggg gagtccagag atttgccttc 2821 aggtcaggga attaataacc tggacaattt aagggattat ttggatggca gtgttaaggt 2881 aaacttagaa aagaaacacc taaataaaag aactcaaata tttccccctg gaatagtcac 2941 catgaatgag tacagtgtgc ctaaaacact gcaggccaga tttgtaaaac aaatagattt 3001 taggcccaaa gattatttaa agcattgcct ggaacgcagt gagtttttgt tagaaaagag 3061 aataattcaa agtggcattg ctttgcttct tatgttaatt tggtacagac ctgtggctga 3121 gtttgctcaa agtattcaga gcagaattgt ggagtggaaa gagagattgg acaaagagtt 3181 tagtttgtca gtgtatcaaa aaatgaagtt taatgtggct atgggaattg gagttttaga 3241 ttggctaaga aacagtgatg atgatgatga agacagccag gaaaatgctg ataaaaatga 3301 agatggtggg gagaagaaca tggaagactc agggcatgaa acaggcattg attcacagtc 3361 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata 3421 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 3481 aacataagga tcccagtgtg gtggtacgta ggaattcgcc agcacagtgg tcgaccctgt 3541 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 3601 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 3661 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 3721 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 3781 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 3841 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc gagtacaagc 3901 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg 3961 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg 4021 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg 4081 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg 4141 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc 4201 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg 4261 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc 4321 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc 4381 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gagtgcccga 4441 aggaccgcgc gacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc 4501 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat 4561 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt 4621 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat 4681 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca 4741 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga 4801 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc 4861 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc 4921 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc 4981 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc 5041 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat 5101 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca 5161 gcgggggtct ttcatttccg acttgtggtc tcgctgcctt gggagggtct cctctgagtg 5221 attgactacc cgtcagcggg ggtcttcaca tgcagcatgt atcaaaatta atttggtttt 5281 ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc caacgcgcgg 5341 ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 5521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 5581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 5641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 5701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 5761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 5821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 5881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 5941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6241 ccttttaaat taaaaatgaa gtttgcggcc gcaaatcaat ctaaagtata tatgagtaaa 6301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 6421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 6481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 6541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 6601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 6661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 6721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 6781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 6841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 6901 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa 6961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac //
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pBABE-Puro SV40LT质粒载体应用举例
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